Natarajan Sethuraman

Summary

Publications

  1. ncbi request reprint Challenges in therapeutic glycoprotein production
    Natarajan Sethuraman
    GlycoFi, Inc, Lebanon, NH 03766, USA
    Curr Opin Biotechnol 17:341-6. 2006
  2. doi request reprint Optimization of erythropoietin production with controlled glycosylation-PEGylated erythropoietin produced in glycoengineered Pichia pastoris
    Juergen H Nett
    GlycoFi, Biologics Discovery, Merck and Co Inc, USA
    J Biotechnol 157:198-206. 2012
  3. ncbi request reprint Humanization of yeast to produce complex terminally sialylated glycoproteins
    Stephen R Hamilton
    GlycoFi Inc, 21 Lafayette Street, Suite 200, Lebanon, NH 03766, USA
    Science 313:1441-3. 2006
  4. doi request reprint High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies
    Gavin C Barnard
    Adimab Inc, 16 Cavendish Court, Lebanon, NH 03766, USA
    J Ind Microbiol Biotechnol 37:961-71. 2010
  5. doi request reprint Improvement of N-glycan site occupancy of therapeutic glycoproteins produced in Pichia pastoris
    Byung Kwon Choi
    GlycoFi, Biologics Discovery, Merck and Co, Inc, 21 Lafayette St, Suite 200, Lebanon, NH 03766, USA
    Appl Microbiol Biotechnol 95:671-82. 2012
  6. doi request reprint Structural elucidation of an α-1,2-mannosidase resistant oligosaccharide produced in Pichia pastoris
    Sujatha Gomathinayagam
    GlycoFi Inc, a Wholly Owned Subsidiary of Merck and Co Inc, 21 Lafayette Street, Suite 200, Lebanon, NH 03766, USA
    Glycobiology 21:1606-15. 2011
  7. doi request reprint Purification process development of a recombinant monoclonal antibody expressed in glycoengineered Pichia pastoris
    Youwei Jiang
    GlycoFi Inc, a Wholly Owned Subsidiary of Merck and Co Inc, 21 Lafayette Street, Suite 200, Lebanon, NH 03766, USA
    Protein Expr Purif 76:7-14. 2011
  8. ncbi request reprint Optimization of humanized IgGs in glycoengineered Pichia pastoris
    Huijuan Li
    GlycoFi Inc 21 Lafayette Street, Lebanon, New Hampshire 03766, USA
    Nat Biotechnol 24:210-5. 2006

Collaborators

  • Huijuan Li
  • Fang Li
  • Thomas I Potgieter
  • Bing Gong
  • Tillman U Gerngross
  • Yan Li
  • Liming Liu
  • Juergen H Nett
  • Christian Heiss
  • Parastoo Azadi
  • Terrance A Stadheim
  • Youwei Jiang
  • Teresa Mitchell
  • Dongxing Zha
  • Byung Kwon Choi
  • Marc D'anjou
  • Sujatha Gomathinayagam
  • Nga Rewa Houston-Cummings
  • Adam Nylen
  • Gavin C Barnard
  • Piotr Bobrowicz
  • Stephen R Hamilton
  • Meehl Meehl
  • Michael Meehl
  • Liza Pon
  • Rohan Patel
  • Istvan Boldogh
  • Shannon Warburton
  • Heping Lin
  • Michael Cukan
  • Erin Giaccone
  • Edward R Zartler
  • Troy W McKelvey
  • Richard J Porambo
  • Carrie L Anderson
  • James E Drummond
  • Renee Moore
  • Heather Lynaugh
  • Alissa M Rittenhour
  • Sandra E Rios
  • Nathan S Sharkey
  • Bianka Prinz
  • Irina Burnina
  • Angela R Kull
  • Seemab S Shaikh
  • Rendall R Strawbridge
  • Harry Wischnewski
  • Jessica Roser
  • Daniel Hopkins
  • Jack Hoopes
  • Robert C Davidson
  • Sandra Rios
  • Stefan Wildt

Detail Information

Publications8

  1. ncbi request reprint Challenges in therapeutic glycoprotein production
    Natarajan Sethuraman
    GlycoFi, Inc, Lebanon, NH 03766, USA
    Curr Opin Biotechnol 17:341-6. 2006
    ..Each of these has different merits for the production of therapeutic glycoproteins and can lead to enhanced therapeutic efficiency...
  2. doi request reprint Optimization of erythropoietin production with controlled glycosylation-PEGylated erythropoietin produced in glycoengineered Pichia pastoris
    Juergen H Nett
    GlycoFi, Biologics Discovery, Merck and Co Inc, USA
    J Biotechnol 157:198-206. 2012
    ..Taken together, our results show that glycoengineered P. pastoris is a suitable production host for rhEPO, yielding an active biologic that is comparable to those produced in current mammalian host systems...
  3. ncbi request reprint Humanization of yeast to produce complex terminally sialylated glycoproteins
    Stephen R Hamilton
    GlycoFi Inc, 21 Lafayette Street, Suite 200, Lebanon, NH 03766, USA
    Science 313:1441-3. 2006
    ..The reported cell lines produce complex glycoproteins with greater than 90% terminal sialylation. Finally, to demonstrate the utility of these yeast strains, functional recombinant erythropoietin was produced...
  4. doi request reprint High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies
    Gavin C Barnard
    Adimab Inc, 16 Cavendish Court, Lebanon, NH 03766, USA
    J Ind Microbiol Biotechnol 37:961-71. 2010
    ..Using this selection process, we were able to isolate a mAb-expressing strain yielding a titer (after protein A purification) in excess of 1 g/l in 0.5-l bioreactors...
  5. doi request reprint Improvement of N-glycan site occupancy of therapeutic glycoproteins produced in Pichia pastoris
    Byung Kwon Choi
    GlycoFi, Biologics Discovery, Merck and Co, Inc, 21 Lafayette St, Suite 200, Lebanon, NH 03766, USA
    Appl Microbiol Biotechnol 95:671-82. 2012
    ..These results suggest that the glycosylation status of recombinant proteins can be improved by heterologous STT3 expression, which will allow for the customization of therapeutic protein profiles...
  6. doi request reprint Structural elucidation of an α-1,2-mannosidase resistant oligosaccharide produced in Pichia pastoris
    Sujatha Gomathinayagam
    GlycoFi Inc, a Wholly Owned Subsidiary of Merck and Co Inc, 21 Lafayette Street, Suite 200, Lebanon, NH 03766, USA
    Glycobiology 21:1606-15. 2011
    ..The difference in enzymatic reactivity can be attributed to multiple β-linkages on the α-1,3 arm of the Man(9)GlcNAc(2) oligosaccharide...
  7. doi request reprint Purification process development of a recombinant monoclonal antibody expressed in glycoengineered Pichia pastoris
    Youwei Jiang
    GlycoFi Inc, a Wholly Owned Subsidiary of Merck and Co Inc, 21 Lafayette Street, Suite 200, Lebanon, NH 03766, USA
    Protein Expr Purif 76:7-14. 2011
    ..5-6.0 efficiently removed these product and process related impurities. The antibody from glycoengineered P. pastoris was comparable to its commercial counterpart in heterotetramer folding, physical stability and binding affinity...
  8. ncbi request reprint Optimization of humanized IgGs in glycoengineered Pichia pastoris
    Huijuan Li
    GlycoFi Inc 21 Lafayette Street, Lebanon, New Hampshire 03766, USA
    Nat Biotechnol 24:210-5. 2006
    ..Glycoengineered P. pastoris provides a general platform for producing recombinant antibodies with human N-glycosylation...