John D Scott



  1. Aggarwal S, Gabrovsek L, Langeberg L, Golkowski M, Ong S, Smith F, et al. Depletion of dAKAP1-protein kinase A signaling islands from the outer mitochondrial membrane alters breast cancer cell metabolism and motility. J Biol Chem. 2019;294:3152-3168 pubmed publisher
    ..We therefore propose that depletion of dAKAP1-PKA "signaling islands" from the outer mitochondrial membrane augments progression toward metastatic breast cancer. ..
  2. Smith F, Omar M, Nygren P, Soughayer J, Hoshi N, Lau H, et al. Single nucleotide polymorphisms alter kinase anchoring and the subcellular targeting of A-kinase anchoring proteins. Proc Natl Acad Sci U S A. 2018;115:E11465-E11474 pubmed publisher
    ..Thus, naturally occurring PKA-anchoring-defective AKAP variants not only perturb dissemination of local second-messenger responses, but also may influence the intracellular distribution of certain AKAP18 isoforms. ..
  3. Scott J, Dessauer C, Tasken K. Creating order from chaos: cellular regulation by kinase anchoring. Annu Rev Pharmacol Toxicol. 2013;53:187-210 pubmed publisher
  4. Smith F, Scott J. Protein kinase A activation: Something new under the sun?. J Cell Biol. 2018;217:1895-1897 pubmed publisher
    ..2018. J. Cell Biol. use antibody tools that selectively recognize phosphorylated RII and the catalytic subunit active site to reexamine PKA holoenzyme activation mechanisms in neurons. ..
  5. Turnham R, Scott J. Protein kinase A catalytic subunit isoform PRKACA; History, function and physiology. Gene. 2016;577:101-8 pubmed publisher
    ..We also explore the potential role of this essential gene as a molecular mediator of certain disease states. ..
  6. Samelson B, Gore B, Whiting J, Nygren P, Purkey A, Colledge M, et al. A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors. J Biol Chem. 2015;290:14107-19 pubmed publisher
    ..1 receptor subtype on serine 1330. These findings imply that anchored PKC locally modulates the phosphorylation status of Robo3.1 in brain regions governing learning and memory and reward. ..
  7. Whiting J, Nygren P, Tunquist B, Langeberg L, Seternes O, Scott J. Protein Kinase A Opposes the Phosphorylation-dependent Recruitment of Glycogen Synthase Kinase 3β to A-kinase Anchoring Protein 220. J Biol Chem. 2015;290:19445-57 pubmed publisher
    ..Occupancy of both R subunit binding sites on AKAP220 could provide a mechanism to amplify local cAMP responses and enable cross-talk between PKA and GSK3β. ..
  8. Nieves Cintrón M, Hirenallur Shanthappa D, Nygren P, Hinke S, Dell Acqua M, Langeberg L, et al. AKAP150 participates in calcineurin/NFAT activation during the down-regulation of voltage-gated K(+) currents in ventricular myocytes following myocardial infarction. Cell Signal. 2016;28:733-40 pubmed publisher
    ..Thus AKAP150-mediated targeting of calcineurin to sarcolemmal micro-domains in ventricular myocytes contributes to the local and acute gene remodeling events that lead to the down-regulation of Kv currents. ..
  9. Whiting J, Ogier L, Forbush K, Bucko P, Gopalan J, Seternes O, et al. AKAP220 manages apical actin networks that coordinate aquaporin-2 location and renal water reabsorption. Proc Natl Acad Sci U S A. 2016;113:E4328-37 pubmed publisher
    ..We propose that membrane-proximal signaling complexes constrained by AKAP220 impact the actin barrier dynamics and AQP2 trafficking to ensure water homeostasis. ..

More Information


  1. Smith F, Esseltine J, Nygren P, Veesler D, Byrne D, Vonderach M, et al. Local protein kinase A action proceeds through intact holoenzymes. Science. 2017;356:1288-1293 pubmed publisher
    ..These findings indicate that the parameters of anchored PKA holoenzyme action are much more restricted than originally anticipated. ..