Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplificationJan P Schouten
MRC Holland, Hudsonstraat 68, 1057SN Amsterdam, The Netherlands
Nucleic Acids Res 30:e57. 2002
..Probe target sequences are small (50-70 nt). The prerequisite of a ligation reaction provides the opportunity to discriminate single nucleotide differences...
Deletion and duplication screening in the DMD gene using MLPATanja Lalic
Department of Medical Genetics, Mother and Child Health Institute of Serbia, Radoja Dakic, Belgrade, Serbia and Montenegro
Eur J Hum Genet 13:1231-4. 2005
..The assay outperforms the Beggs and Chamberlain multiplex-PCR test, and should be considered as the method of choice for an initial DNA analysis of DMD/BMD patients...
A novel approach to simultaneously scan genes at fragile sitesPascale Willem
Department of Hematology and Molecular Medicine University of the Witwatersrand and the National Health Laboratory Services, Wits Medical School, 8 York road, 2193 Parktown, South Africa
BMC Cancer 6:205. 2006
..The loci for FHIT and WWOX both span over a megabase but the genes encode for small transcripts. Thus the screening of intragenic deletion can be difficult and has relied on loss of heterozygosity LOH assays, or genomic arrays...
- Large genomic deletions and duplications in the BRCA1 gene identified by a novel quantitative method
Frans B L Hogervorst
Family Cancer Clinic, Netherlands Cancer Institute, Amsterdam, The Netherlands
Cancer Res 63:1449-53. 2003
..The MLPA test for BRCA1 ensures a sensitive and comprehensive high-throughput screening test for genomic rearrangement and can easily be implemented in the molecular analysis of BRCA1...
- Delineating genetic pathways of disease progression in head and neck squamous cell carcinoma
Maria J Worsham
Department of Otolaryngology Head and Neck Surgery, Research Division, Henry Ford Health System, Detroit, MI 48202, USA
Arch Otolaryngol Head Neck Surg 129:702-8. 2003
..Identification of key molecular events may be useful for more accurate distinction of prognostic groups for selection and targeting of therapy...
- Epigenetic events of disease progression in head and neck squamous cell carcinoma
Maria J Worsham
Department of Otolaryngology Head and Neck Surgery and Research Division, Henry Ford Health System, Detroit, Mich, USA
Arch Otolaryngol Head Neck Surg 132:668-77. 2006
..To examine the promoter methylation status of the 22 cancer genes and their contribution to disease progression in 6 head and neck squamous cell carcinoma (HNSCC) cell lines...
- Rapid and quantitative detection of homologous and non-homologous recombination events using three oligonucleotide MLPA
Petra Langerak
Division of Immunology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
Nucleic Acids Res 33:e188. 2005
..By using MLPA probes consisting of three oligonucleotides we were able to simultaneously detect and quantify both wild-type and mutant alleles...
- Methylation-specific MLPA (MS-MLPA): simultaneous detection of CpG methylation and copy number changes of up to 40 sequences
Anders O H Nygren
MRC-Holland bv Amsterdam, The Netherlands
Nucleic Acids Res 33:e128. 2005
..To validate this novel method, we used MS-MLPA to detect aberrant methylation in DNA samples of patients with Prader-Willy syndrome, Angelman syndrome or acute myeloid leukemia...
- A disintegrin and metalloprotease 33 polymorphisms and lung function decline in the general population
Cleo C van Diemen
Department of Epidemiology and Bioinformatics, University of Groningen, The Netherlands
Am J Respir Crit Care Med 172:329-33. 2005
....
- Pharmacogenomics and outcome of asthma: no clinical application for long-term steroid effects by CRHR1 polymorphisms
Antoon Dijkstra
J Allergy Clin Immunol 121:1510-3. 2008
