D A Schofield

Summary

Publications

  1. doi request reprint Bacillus anthracis diagnostic detection and rapid antibiotic susceptibility determination using 'bioluminescent' reporter phage
    David A Schofield
    Guild Associates, Inc, Charleston, SC 29407, United States Electronic address
    J Microbiol Methods 95:156-61. 2013
  2. pmc "Light-tagged" bacteriophage as a diagnostic tool for the detection of phytopathogens
    David Schofield
    Guild Associates, Inc, Charleston, SC USA
    Bioengineered 4:50-4. 2013
  3. pmc Rapid identification and antibiotic susceptibility testing of Yersinia pestis using bioluminescent reporter phage
    David A Schofield
    Guild Associates, Inc, Charleston, SC 29407, USA
    J Microbiol Methods 90:80-2. 2012
  4. pmc Development of an engineered bioluminescent reporter phage for detection of bacterial blight of crucifers
    David A Schofield
    Guild Associates, Inc, Charleston, South Carolina, USA
    Appl Environ Microbiol 78:3592-8. 2012
  5. doi request reprint Generation of a mutagenized organophosphorus hydrolase for the biodegradation of the organophosphate pesticides malathion and demeton-S
    D A Schofield
    Guild Associates Inc, Charleston, SC 29407, USA
    J Appl Microbiol 109:548-57. 2010
  6. pmc Diagnostic bioluminescent phage for detection of Yersinia pestis
    David A Schofield
    Guild Associates Inc, Charleston, South Carolina 29407, USA
    J Clin Microbiol 47:3887-94. 2009
  7. doi request reprint Phage-mediated bioluminescent detection of Bacillus anthracis
    D A Schofield
    Guild Associates Inc, Charleston, South Carolina 29407, USA
    J Appl Microbiol 107:1468-78. 2009
  8. ncbi request reprint Development of a yeast biosensor-biocatalyst for the detection and biodegradation of the organophosphate paraoxon
    David A Schofield
    Guild Associates Inc, 1313B Ashley River Road, Charleston, SC 29407, USA
    Appl Microbiol Biotechnol 76:1383-94. 2007
  9. ncbi request reprint Hydrolytic gene expression during oroesophageal and gastric candidiasis in immunocompetent and immunodeficient gnotobiotic mice
    David A Schofield
    Division of Mycology, Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29403, USA
    J Infect Dis 188:591-9. 2003
  10. pmc Use of genetically engineered phage to deliver antimicrobial agents to bacteria: an alternative therapy for treatment of bacterial infections
    Caroline Westwater
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29403, USA
    Antimicrob Agents Chemother 47:1301-7. 2003

Research Grants

  1. Identifying targets for diagnosing oral candidiasis
    David Schofield; Fiscal Year: 2005

Detail Information

Publications14

  1. doi request reprint Bacillus anthracis diagnostic detection and rapid antibiotic susceptibility determination using 'bioluminescent' reporter phage
    David A Schofield
    Guild Associates, Inc, Charleston, SC 29407, United States Electronic address
    J Microbiol Methods 95:156-61. 2013
    ..Wß::luxAB rapidly detects B. anthracis and determines antibiotic efficacy, properties that will help patient outcome...
  2. pmc "Light-tagged" bacteriophage as a diagnostic tool for the detection of phytopathogens
    David Schofield
    Guild Associates, Inc, Charleston, SC USA
    Bioengineered 4:50-4. 2013
    ..The reporter phage displays promise for the rapid and specific diagnostic detection of cultivated isolates, and infected plant specimens...
  3. pmc Rapid identification and antibiotic susceptibility testing of Yersinia pestis using bioluminescent reporter phage
    David A Schofield
    Guild Associates, Inc, Charleston, SC 29407, USA
    J Microbiol Methods 90:80-2. 2012
    ..The phage-generated susceptibility profiles, however, were obtained within hours in contrast to days with the conventional method...
  4. pmc Development of an engineered bioluminescent reporter phage for detection of bacterial blight of crucifers
    David A Schofield
    Guild Associates, Inc, Charleston, South Carolina, USA
    Appl Environ Microbiol 78:3592-8. 2012
    ..Importantly, the reporter phage detects P. cannabina pv. alisalensis on diseased plant specimens, indicating its potential for disease diagnosis...
  5. doi request reprint Generation of a mutagenized organophosphorus hydrolase for the biodegradation of the organophosphate pesticides malathion and demeton-S
    D A Schofield
    Guild Associates Inc, Charleston, SC 29407, USA
    J Appl Microbiol 109:548-57. 2010
    ..The aim of this study was to generate a variant OPH enzyme with improved hydrolytic efficiency against the poorly hydrolysed P-S class of organophosphates...
  6. pmc Diagnostic bioluminescent phage for detection of Yersinia pestis
    David A Schofield
    Guild Associates Inc, Charleston, South Carolina 29407, USA
    J Clin Microbiol 47:3887-94. 2009
    ..Collectively, the results indicate that the reporter phage displays promise for the rapid and specific diagnostic detection of cultivated Y. pestis isolates or infected clinical specimens...
  7. doi request reprint Phage-mediated bioluminescent detection of Bacillus anthracis
    D A Schofield
    Guild Associates Inc, Charleston, South Carolina 29407, USA
    J Appl Microbiol 107:1468-78. 2009
    ..The aim of this study was to provide the proof of principle results for the development of a 'bioluminescent' reporter bacteriophage that was capable of specifically detecting B. anthracis...
  8. ncbi request reprint Development of a yeast biosensor-biocatalyst for the detection and biodegradation of the organophosphate paraoxon
    David A Schofield
    Guild Associates Inc, 1313B Ashley River Road, Charleston, SC 29407, USA
    Appl Microbiol Biotechnol 76:1383-94. 2007
    ..Together, these results indicate that the yeast biocatalyst-biosensor can detect and degrade paraoxon and potentially also monitor the decontamination process...
  9. ncbi request reprint Hydrolytic gene expression during oroesophageal and gastric candidiasis in immunocompetent and immunodeficient gnotobiotic mice
    David A Schofield
    Division of Mycology, Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29403, USA
    J Infect Dis 188:591-9. 2003
    ....
  10. pmc Use of genetically engineered phage to deliver antimicrobial agents to bacteria: an alternative therapy for treatment of bacterial infections
    Caroline Westwater
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29403, USA
    Antimicrob Agents Chemother 47:1301-7. 2003
    ..Given the powerful genetic engineering tools available and the present knowledge in phage biology, this technology may have potential use in antimicrobial therapies and DNA vaccine development...
  11. pmc Candida albicans-conditioned medium protects yeast cells from oxidative stress: a possible link between quorum sensing and oxidative stress resistance
    Caroline Westwater
    Center for Oral Health Research, Department of Stomatology, Medical University of South Carolina, Charleston, USA
    Eukaryot Cell 4:1654-61. 2005
    ..Together, these data suggest a link between the quorum-sensing molecule farnesol and the oxidative stress response in C. albicans...
  12. ncbi request reprint Differential Candida albicans lipase gene expression during alimentary tract colonization and infection
    David A Schofield
    Department of Microbiology, Medical University of South Carolina, Charleston, SC, USA
    FEMS Microbiol Lett 244:359-65. 2005
    ..Therefore, differential LIP gene expression was observed during colonization, infection and at different infected mucosal sites...
  13. ncbi request reprint Development of a P1 phagemid system for the delivery of DNA into Gram-negative bacteria
    Caroline Westwater
    Department of Microbiology and Immunology, Medical University of South Carolina, 173 Ashley Avenue BSB 201, Charleston, SC 29403, USA
    Microbiology 148:943-50. 2002
    ..This ability to deliver genetic information to wild-type strains raises the potential for use in antimicrobial therapies and DNA vaccine development...

Research Grants1

  1. Identifying targets for diagnosing oral candidiasis
    David Schofield; Fiscal Year: 2005
    ..Since Candida species are the most important fungal pathogen of the oral cavity, the rapid and accurate diagnosis of oral candidiasis using novel disease biomarkers will significantly improve oral health. ..