gamma-tubulin complexes: binding to the centrosome, regulation and microtubule nucleationE Schiebel
Beatson Laboratories, The Beatson Institute for Cancer Research, Cancer Research Campaign, Glasgow, G61 1BD, UK UK
Curr Opin Cell Biol 12:113-8. 2000
..Furthermore, it was found that the small GTPase Ran in its GDP-bound state may promote spindle assembly. In addition, an essential function of gamma-tubulin in basal body duplication has been demonstrated in Paramecium...
- Regulating microtubule properties by modifying their organizing minus ends
T Usui
The Beatson Institute for Cancer Research, CRC Beatson Laboratories, G61 1BD, Glasgow, United Kingdom
Mol Cell 8:931-2. 2001
..describe that the budding yeast gamma-tubulin, Tub4p, is phosphorylated at a conserved tyrosine in G1 phase of the cell cycle. The results suggest that gamma-tubulin phosphorylation regulates the number and dynamics of microtubules...
- The Bub2p spindle checkpoint links nuclear migration with mitotic exit
G Pereira
The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow, United Kingdom
Mol Cell 6:1-10. 2000
..Since components of the Bub2p checkpoint are conserved in other organisms, we propose that the position of the SPB or mammalian centrosome controls the timing of mitotic exit...
Interaction of the yeast gamma-tubulin complex-binding protein Spc72p with Kar1p is essential for microtubule function during karyogamyG Pereira
The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, Scotland, UK
EMBO J 18:4180-95. 1999
..Taken together, these observations suggest that the relocalization of Spc72p within the spindle pole body is the 'landmark' event in the pheromone-induced reorganization of the cytoplasmic microtubules...
- Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation
M Knop
The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
EMBO J 17:3952-67. 1998
..These results suggest that different components of MTOCs act as receptors for gamma-tubulin complexes and that they are essential for the function of MTOCs...
- Spc98p directs the yeast gamma-tubulin complex into the nucleus and is subject to cell cycle-dependent phosphorylation on the nuclear side of the spindle pole body
G Pereira
Max Planck Institut fur Biochemie, 82152 Martinsried, Germany
Mol Biol Cell 9:775-93. 1998
..The kinase Mps1p, which functions in SPB duplication and mitotic checkpoint control, seems to be involved in Spc98p phosphorylation. Our results also suggest that the nuclear and cytoplasmic Tub4p complexes are regulated differently...
- Spc98p and Spc97p of the yeast gamma-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p
M Knop
Max Planck Institut fur Biochemie, Genzentrum, Am Klopferspitz 18a, 82152 Martinsried, Germany
EMBO J 16:6985-95. 1997
..We discuss in a model, how the N-terminus of Spc110p anchors the Tub4p complex to the SPB and how Spc110p itself is embedded in the SPB...
- The spindle pole body component Spc97p interacts with the gamma-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication
M Knop
Max Planck Institut fur Biochemie, Martinsried, Germany
EMBO J 16:1550-64. 1997
..We discuss a model in which the Tub4p-Spc98p-Spc97p complex is part of the microtubule attachment site at the SPB...
- Nud1p links astral microtubule organization and the control of exit from mitosis
U Gruneberg
The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, UK
EMBO J 19:6475-88. 2000
..Thus, in nud1-2 cells the failure of Tem1p to interact with Cdc15p at the SPB probably prevents mitotic exit...
- gamma-Tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation
A Spang
Max Planck Institut fur Biochemie, Genzentrum, Martinsried, Germany
J Cell Biol 134:429-41. 1996
..In addition, cell cycle arrest and survival of tub4-1 cells is dependent on the mitotic checkpoint control gene BUB2 (Hoyt, M.A., L. Totis, B.T. Roberts. 1991. Cell. 66:507-517), one of the cell's monitors of spindle integrity...
- The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control
C Janke
The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, UK
EMBO J 20:777-91. 2001
..Since Ndc80p, Nuf2p and Spc24p are conserved proteins, it is likely that similar complexes are part of the kinetochore in other organisms...
- The role of the yeast spindle pole body and the mammalian centrosome in regulating late mitotic events
G Pereira
The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, UK
Curr Opin Cell Biol 13:762-9. 2001
..Elucidation of the molecular roles played by these regulators is helping to clarify the function of the SPB in controlling progression though mitosis...
- Modes of spindle pole body inheritance and segregation of the Bfa1p-Bub2p checkpoint protein complex
G Pereira
The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, UK
EMBO J 20:6359-70. 2001
..This may provide a mechanism to delay cell cycle progression when cytoplasmic microtubules fail to orient the spindle. Thus, SPBs are able to sense cytoplasmic microtubule properties and regulate the Bfa1p-Bub2p GAP accordingly...
- The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication
C Schramm
The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, UK
EMBO J 19:421-33. 2000
..In addition, we found that Bbp1p interacts with Spc29p and the half-bridge component Kar1p. These interactions are likely to play a role in connecting the SPB with the nuclear envelope and the central plaque with the half-bridge...
- MtGimC, a novel archaeal chaperone related to the eukaryotic chaperonin cofactor GimC/prefoldin
M R Leroux
Max Planck Institut fur Biochemie, Department of Cellular Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
EMBO J 18:6730-43. 1999
..In light of the absence of Hsp70 chaperones in many archaea, GimC may fulfil an ATP-independent, Hsp70-like function in archaeal de novo protein folding...
- Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication
S Elliott
Beatson Institute for Cancer Research, Cancer Research Campaign Beatson Laboratories, Glasgow G61 1BD, United Kingdom
Proc Natl Acad Sci U S A 96:6205-10. 1999
....
- Binding of centrins and yeast calmodulin to synthetic peptides corresponding to binding sites in the spindle pole body components Kar1p and Spc110p
B M Geier
Max Planck Institut fur Biochemie, Genzentrum, Am Klopferspitz 18a, D 82152 Martinsried, Germany
J Biol Chem 271:28366-74. 1996
..Our results suggest that centrins are involved in Ca2+ signaling, likely by influencing the properties of target proteins in response to changes in [Ca2+]...
- The spacer protein Spc110p targets calmodulin to the central plaque of the yeast spindle pole body
A Spang
Max Planck Institut fur Biochemie, Genzentrum, Martinsried, Germany
J Cell Sci 109:2229-37. 1996
..In addition, synthetic peptides corresponding to the calmodulin-binding site of Spc110p bound to calmodulin with a Kd in the nanomolar range and nearly independent of Ca2+...
- Characterization of green alga, yeast, and human centrins. Specific subdomain features determine functional diversity
H Wiech
Max Planck Institut fur Biochemie, Genzentrum, Am Klopferspitz 18a, 82152 Martinsried, Federal Republic of Germany
J Biol Chem 271:22453-61. 1996
..Only hybrid proteins containing the amino-terminal subdomain or the third EF-hand of SdCen and the other subdomains from Cdc31p were functional in vivo...
- The spindle pole body component Spc98p interacts with the gamma-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment
S Geissler
Max Planck Institut fur Biochemie, Am Klopferspitz 18a, Genzentrum, 82152 Martinsried, Germany
EMBO J 15:3899-911. 1996
..In addition, Spc98-1p is defective in its interaction with Tub4p in the two-hybrid system. We suggest a model in which Tub4p and Spc98p form a complex involved in microtubule organization by the SPB...
- The calcium-binding protein cell division cycle 31 of Saccharomyces cerevisiae is a component of the half bridge of the spindle pole body
A Spang
Max Planck Institut fur Biochemie, Genzentrum, Martinsried, Germany
J Cell Biol 123:405-16. 1993
..We suggest that CDC31 is a member of a family of calcium-binding, centrosome-associated proteins from a phylogenetically diverse group of organisms...
- The Cdc31p-binding protein Kar1p is a component of the half bridge of the yeast spindle pole body
A Spang
Max Planck Institut fur Biochemie, Genzentrum, Martinsried, Germany
J Cell Biol 128:863-77. 1995
..V., S. L. Dyos, D. Kershaw, and J. T. Finch. 1993. J. Cell Biol. 123:1175-1184). The association of Kar1p with the spindle pole body was independent of Cdc31p. Cdc31p, on the other hand, was not associated with SPBs of kar1 cells...
