E Schiebel

Summary

Publications

  1. ncbi request reprint gamma-tubulin complexes: binding to the centrosome, regulation and microtubule nucleation
    E Schiebel
    Beatson Laboratories, The Beatson Institute for Cancer Research, Cancer Research Campaign, Glasgow, G61 1BD, UK UK
    Curr Opin Cell Biol 12:113-8. 2000
  2. ncbi request reprint Regulating microtubule properties by modifying their organizing minus ends
    T Usui
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, G61 1BD, Glasgow, United Kingdom
    Mol Cell 8:931-2. 2001
  3. ncbi request reprint The Bub2p spindle checkpoint links nuclear migration with mitotic exit
    G Pereira
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow, United Kingdom
    Mol Cell 6:1-10. 2000
  4. pmc Interaction of the yeast gamma-tubulin complex-binding protein Spc72p with Kar1p is essential for microtubule function during karyogamy
    G Pereira
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, Scotland, UK
    EMBO J 18:4180-95. 1999
  5. pmc Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation
    M Knop
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    EMBO J 17:3952-67. 1998
  6. pmc Spc98p directs the yeast gamma-tubulin complex into the nucleus and is subject to cell cycle-dependent phosphorylation on the nuclear side of the spindle pole body
    G Pereira
    Max Planck Institut fur Biochemie, 82152 Martinsried, Germany
    Mol Biol Cell 9:775-93. 1998
  7. pmc Spc98p and Spc97p of the yeast gamma-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p
    M Knop
    Max Planck Institut fur Biochemie, Genzentrum, Am Klopferspitz 18a, 82152 Martinsried, Germany
    EMBO J 16:6985-95. 1997
  8. pmc The spindle pole body component Spc97p interacts with the gamma-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication
    M Knop
    Max Planck Institut fur Biochemie, Martinsried, Germany
    EMBO J 16:1550-64. 1997
  9. pmc Nud1p links astral microtubule organization and the control of exit from mitosis
    U Gruneberg
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, UK
    EMBO J 19:6475-88. 2000
  10. pmc gamma-Tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation
    A Spang
    Max Planck Institut fur Biochemie, Genzentrum, Martinsried, Germany
    J Cell Biol 134:429-41. 1996

Collaborators

  • M Knop
  • K Nasmyth
  • G Pereira
  • A Spang
  • C Schramm
  • S Elliott
  • K Grein
  • C Janke
  • T Usui
  • A Shevchenko
  • U Gruneberg
  • J Grindlay
  • M R Leroux
  • B M Geier
  • H Wiech
  • S Geissler
  • M M Magiera
  • J Lechner
  • T U Tanaka
  • J Ortiz
  • T Höfken
  • K Campbell
  • C Manson
  • C Simpson
  • K Siegers
  • C M Dobson
  • F U Hartl
  • D Klunker
  • G Schlenstedt
  • A N Lupas
  • U Grueneberg
  • M Fändrich
  • J R Brown
  • M Matzner
  • I Courtney
  • S Soues
  • J Kilmartin
  • T Paschke
  • M Melkonian
  • J Steinkötter
  • U Fackler

Detail Information

Publications22

  1. ncbi request reprint gamma-tubulin complexes: binding to the centrosome, regulation and microtubule nucleation
    E Schiebel
    Beatson Laboratories, The Beatson Institute for Cancer Research, Cancer Research Campaign, Glasgow, G61 1BD, UK UK
    Curr Opin Cell Biol 12:113-8. 2000
    ..Furthermore, it was found that the small GTPase Ran in its GDP-bound state may promote spindle assembly. In addition, an essential function of gamma-tubulin in basal body duplication has been demonstrated in Paramecium...
  2. ncbi request reprint Regulating microtubule properties by modifying their organizing minus ends
    T Usui
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, G61 1BD, Glasgow, United Kingdom
    Mol Cell 8:931-2. 2001
    ..describe that the budding yeast gamma-tubulin, Tub4p, is phosphorylated at a conserved tyrosine in G1 phase of the cell cycle. The results suggest that gamma-tubulin phosphorylation regulates the number and dynamics of microtubules...
  3. ncbi request reprint The Bub2p spindle checkpoint links nuclear migration with mitotic exit
    G Pereira
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow, United Kingdom
    Mol Cell 6:1-10. 2000
    ..Since components of the Bub2p checkpoint are conserved in other organisms, we propose that the position of the SPB or mammalian centrosome controls the timing of mitotic exit...
  4. pmc Interaction of the yeast gamma-tubulin complex-binding protein Spc72p with Kar1p is essential for microtubule function during karyogamy
    G Pereira
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, Scotland, UK
    EMBO J 18:4180-95. 1999
    ..Taken together, these observations suggest that the relocalization of Spc72p within the spindle pole body is the 'landmark' event in the pheromone-induced reorganization of the cytoplasmic microtubules...
  5. pmc Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation
    M Knop
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    EMBO J 17:3952-67. 1998
    ..These results suggest that different components of MTOCs act as receptors for gamma-tubulin complexes and that they are essential for the function of MTOCs...
  6. pmc Spc98p directs the yeast gamma-tubulin complex into the nucleus and is subject to cell cycle-dependent phosphorylation on the nuclear side of the spindle pole body
    G Pereira
    Max Planck Institut fur Biochemie, 82152 Martinsried, Germany
    Mol Biol Cell 9:775-93. 1998
    ..The kinase Mps1p, which functions in SPB duplication and mitotic checkpoint control, seems to be involved in Spc98p phosphorylation. Our results also suggest that the nuclear and cytoplasmic Tub4p complexes are regulated differently...
  7. pmc Spc98p and Spc97p of the yeast gamma-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p
    M Knop
    Max Planck Institut fur Biochemie, Genzentrum, Am Klopferspitz 18a, 82152 Martinsried, Germany
    EMBO J 16:6985-95. 1997
    ..We discuss in a model, how the N-terminus of Spc110p anchors the Tub4p complex to the SPB and how Spc110p itself is embedded in the SPB...
  8. pmc The spindle pole body component Spc97p interacts with the gamma-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication
    M Knop
    Max Planck Institut fur Biochemie, Martinsried, Germany
    EMBO J 16:1550-64. 1997
    ..We discuss a model in which the Tub4p-Spc98p-Spc97p complex is part of the microtubule attachment site at the SPB...
  9. pmc Nud1p links astral microtubule organization and the control of exit from mitosis
    U Gruneberg
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, UK
    EMBO J 19:6475-88. 2000
    ..Thus, in nud1-2 cells the failure of Tem1p to interact with Cdc15p at the SPB probably prevents mitotic exit...
  10. pmc gamma-Tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation
    A Spang
    Max Planck Institut fur Biochemie, Genzentrum, Martinsried, Germany
    J Cell Biol 134:429-41. 1996
    ..In addition, cell cycle arrest and survival of tub4-1 cells is dependent on the mitotic checkpoint control gene BUB2 (Hoyt, M.A., L. Totis, B.T. Roberts. 1991. Cell. 66:507-517), one of the cell's monitors of spindle integrity...
  11. pmc The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control
    C Janke
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, UK
    EMBO J 20:777-91. 2001
    ..Since Ndc80p, Nuf2p and Spc24p are conserved proteins, it is likely that similar complexes are part of the kinetochore in other organisms...
  12. ncbi request reprint The role of the yeast spindle pole body and the mammalian centrosome in regulating late mitotic events
    G Pereira
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, UK
    Curr Opin Cell Biol 13:762-9. 2001
    ..Elucidation of the molecular roles played by these regulators is helping to clarify the function of the SPB in controlling progression though mitosis...
  13. pmc Modes of spindle pole body inheritance and segregation of the Bfa1p-Bub2p checkpoint protein complex
    G Pereira
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, UK
    EMBO J 20:6359-70. 2001
    ..This may provide a mechanism to delay cell cycle progression when cytoplasmic microtubules fail to orient the spindle. Thus, SPBs are able to sense cytoplasmic microtubule properties and regulate the Bfa1p-Bub2p GAP accordingly...
  14. pmc The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication
    C Schramm
    The Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow G61 1BD, UK
    EMBO J 19:421-33. 2000
    ..In addition, we found that Bbp1p interacts with Spc29p and the half-bridge component Kar1p. These interactions are likely to play a role in connecting the SPB with the nuclear envelope and the central plaque with the half-bridge...
  15. pmc MtGimC, a novel archaeal chaperone related to the eukaryotic chaperonin cofactor GimC/prefoldin
    M R Leroux
    Max Planck Institut fur Biochemie, Department of Cellular Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    EMBO J 18:6730-43. 1999
    ..In light of the absence of Hsp70 chaperones in many archaea, GimC may fulfil an ATP-independent, Hsp70-like function in archaeal de novo protein folding...
  16. pmc Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication
    S Elliott
    Beatson Institute for Cancer Research, Cancer Research Campaign Beatson Laboratories, Glasgow G61 1BD, United Kingdom
    Proc Natl Acad Sci U S A 96:6205-10. 1999
    ....
  17. ncbi request reprint Binding of centrins and yeast calmodulin to synthetic peptides corresponding to binding sites in the spindle pole body components Kar1p and Spc110p
    B M Geier
    Max Planck Institut fur Biochemie, Genzentrum, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Biol Chem 271:28366-74. 1996
    ..Our results suggest that centrins are involved in Ca2+ signaling, likely by influencing the properties of target proteins in response to changes in [Ca2+]...
  18. ncbi request reprint The spacer protein Spc110p targets calmodulin to the central plaque of the yeast spindle pole body
    A Spang
    Max Planck Institut fur Biochemie, Genzentrum, Martinsried, Germany
    J Cell Sci 109:2229-37. 1996
    ..In addition, synthetic peptides corresponding to the calmodulin-binding site of Spc110p bound to calmodulin with a Kd in the nanomolar range and nearly independent of Ca2+...
  19. ncbi request reprint Characterization of green alga, yeast, and human centrins. Specific subdomain features determine functional diversity
    H Wiech
    Max Planck Institut fur Biochemie, Genzentrum, Am Klopferspitz 18a, 82152 Martinsried, Federal Republic of Germany
    J Biol Chem 271:22453-61. 1996
    ..Only hybrid proteins containing the amino-terminal subdomain or the third EF-hand of SdCen and the other subdomains from Cdc31p were functional in vivo...
  20. pmc The spindle pole body component Spc98p interacts with the gamma-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment
    S Geissler
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, Genzentrum, 82152 Martinsried, Germany
    EMBO J 15:3899-911. 1996
    ..In addition, Spc98-1p is defective in its interaction with Tub4p in the two-hybrid system. We suggest a model in which Tub4p and Spc98p form a complex involved in microtubule organization by the SPB...
  21. pmc The calcium-binding protein cell division cycle 31 of Saccharomyces cerevisiae is a component of the half bridge of the spindle pole body
    A Spang
    Max Planck Institut fur Biochemie, Genzentrum, Martinsried, Germany
    J Cell Biol 123:405-16. 1993
    ..We suggest that CDC31 is a member of a family of calcium-binding, centrosome-associated proteins from a phylogenetically diverse group of organisms...
  22. pmc The Cdc31p-binding protein Kar1p is a component of the half bridge of the yeast spindle pole body
    A Spang
    Max Planck Institut fur Biochemie, Genzentrum, Martinsried, Germany
    J Cell Biol 128:863-77. 1995
    ..V., S. L. Dyos, D. Kershaw, and J. T. Finch. 1993. J. Cell Biol. 123:1175-1184). The association of Kar1p with the spindle pole body was independent of Cdc31p. Cdc31p, on the other hand, was not associated with SPBs of kar1 cells...