Anatoly Uzdensky

Summary

Affiliation: Southern Federal University
Location: Rostov-on-Don, Russia
Summary:
Photodynamic effect on neurons and glial cells
Cell death: necrosis and apoptosis
Signaling
Neuroglial interactions
Ultrastructure

Publications:
1: Komandirov MA, Knyazeva EA, Fedorenko YP, Rudkovskii MV, Stetsurin DA,
Uzdensky AB. On the Role of Phosphatidylinositol 3-Kinase, Protein Kinase B/Akt,
and Glycogen Synthase Kinase-3β in Photodynamic Injury of Crayfish Neurons and
Glial Cells. J Mol Neurosci. 2011 Feb 12. [Epub ahead of print] PubMed PMID:
.


2: Fedorenko GM, Fedorenko YP, Fedorenko AG, Uzdensky AB. Dynamics of
ultrastructural alterations in photosensitized crayfish glial and neuronal cells:
Structures involved in transport processes and neuroglial interactions. J
Neurosci Res. 2011 Mar;89(3):341-51. doi: 10.1002/jnr.22560. Epub 2010 Dec 22.
PubMed PMID: .


3: Fedorenko GM, Fedorenko YP, Fedorenko AG, Uzdensky AB. Dynamics of
ultrastructural alterations in photosensitized crayfish glial and neuronal cells:
Structures involved in transport processes and neuroglial interactions. J
Neurosci Res. 2010 Dec 22. [Epub ahead of print] PubMed PMID: .


4: Fedorenko GM, Uzdensky AB. Cellular structures involved in the transport
processes and neuroglial interactions in the crayfish stretch receptor. J Integr
Neurosci. 2009 Dec;8(4):433-40. PubMed PMID: .


5: Uzdensky AB, Lobanov AV. Elevated activity of the crayfish stretch receptor
neuron increases resistance of surrounding glial cells to apoptosis induced by
photodynamic treatment. Neurosci Lett. 2010 Jan 1;468(1):89-92. Epub 2009 Oct 29.
PubMed PMID: .


6: Fedorenko GM, Uzdensky AB. Ultrastructure of neuroglial contacts in crayfish
stretch receptor. Cell Tissue Res. 2009 Sep;337(3):477-90. Epub 2009 Jul 8.
PubMed PMID: .


7: Lobanov AV, Uzdensky AB. Protection of crayfish glial cells but not neurons
from photodynamic injury by nerve growth factor. J Mol Neurosci. 2009
Sep;39(1-2):308-19. Epub 2009 Apr 18. PubMed PMID: .


8: Fedorenko GM, Uzdensky AB. Dynamics of ultrastructural changes in the isolated
crayfish mechanoreceptor neuron under photodynamic impact. J Neurosci Res. 2008
May 1;86(6):1409-16. PubMed PMID: .


9: Uzdensky A, Lobanov A, Bibov M, Petin Y. Involvement of Ca2+- and cyclic
adenosine monophosphate-mediated signaling pathways in photodynamic injury of
isolated crayfish neuron and satellite glial cells. J Neurosci Res. 2007
Mar;85(4):860-70. PubMed PMID: .


10: Kolosov M, Uzdensky A. Crayfish mechanoreceptor neuron prevents photoinduced
apoptosis of satellite glial cells. Brain Res Bull. 2006 May 15;69(5):495-500.
Epub 2006 Mar 24. PubMed PMID: .


11: Lobanov AV, Uzdensky AB. PDT-induced death of sensory neurons and glial cells
in the isolated crayfish stretch receptor after proteolytic treatment. J Neurosci
Res. 2005 Dec 15;82(6):866-74. PubMed PMID: .


12: Uzdensky A, Kolpakova E, Juzeniene A, Juzenas P, Moan J. The effect of
sub-lethal ALA-PDT on the cytoskeleton and adhesion of cultured human cancer
cells. Biochim Biophys Acta. 2005 Feb 11;1722(1):43-50. Epub 2004 Dec 15. PubMed
PMID: .


13: Uzdensky A, Kolosov M, Bragin D, Dergacheva O, Vanzha O, Oparina L.
Involvement of adenylate cyclase and tyrosine kinase signaling pathways in
response of crayfish stretch receptor neuron and satellite glia cell to
photodynamic treatment. Glia. 2005 Feb;49(3):339-48. PubMed PMID: .


14: Uzdensky AB, Juzeniene A, Kolpakova E, Hjortland GO, Juzenas P, Moan J.
Photosensitization with protoporphyrin IX inhibits attachment of cancer cells to
a substratum. Biochem Biophys Res Commun. 2004 Sep 17;322(2):452-7. PubMed PMID:
.


15: Uzdensky AB, Dergacheva OY, Zhavoronkova AA, Reshetnikov AV, Ponomarev GV.
Photodynamic effect of novel chlorin e6 derivatives on a single nerve cell. Life
Sci. 2004 Mar 12;74(17):2185-97. PubMed PMID: .


16: Uzdensky A, Juzeniene A, Ma LW, Moan J. Photodynamic inhibition of enzymatic
detachment of human cancer cells from a substratum. Biochim Biophys Acta. 2004
Jan 5;1670(1):1-11. PubMed PMID: .


17: Uzdensky AB, Bragin DE, Kolosov MS, Kubin A, Loew HG, Moan J. Photodynamic
effect of hypericin and a water-soluble derivative on isolated crayfish neuron
and surrounding glial cells. J Photochem Photobiol B. 2003 Dec 5;72(1-3):27-33.
PubMed PMID: .


18: Bragin DE, Kolosov MS, Uzdensky AB. Photodynamic inactivation of isolated
crayfish neuron requires protein kinase C, PI 3-kinase and Ca2+. J Photochem
Photobiol B. 2003 May-Jun;70(2):99-105. PubMed PMID: .


19: Uzdensky A, Bragin D, Kolosov M, Dergacheva O, Fedorenko G, Zhavoronkova A.
Photodynamic inactivation of isolated crayfish mechanoreceptor neuron: different
death modes under different photosensitizer concentrations. Photochem Photobiol.
2002 Oct;76(4):431-7. PubMed PMID: .


20: Uzdensky AB, Iani V, Ma LW, Moan J. Photobleaching of hypericin bound to
human serum albumin, cultured adenocarcinoma cells and nude mice skin. Photochem
Photobiol. 2002 Sep;76(3):320-8. PubMed PMID: .


21: Uzdensky AB, Ma LW, Iani V, Hjortland GO, Steen HB, Moan J. Intracellular
localisation of hypericin in human glioblastoma and carcinoma cell lines. Lasers
Med Sci. 2001;16(4):276-83. PubMed PMID: .


22: Uzdensky AB, Dergacheva OY, Zhavoronkova AA, Ivanov AV, Reshetnikov AV,
Ponomarev GV. Photodynamic effect of deuteroporphyrin IX and hematoporphyrin
derivatives on single neuron. Biochem Biophys Res Commun. 2001 Mar;281(5):1194-9.
PubMed PMID: .


23: Uzdensky AB, Derkacheva VM, Dergacheva OY, Zhavoronkova AA. A single neuron
response to photodynamic effect of various aluminum and zinc phthalocyanines.
Life Sci. 2000 Dec 22;68(5):547-55. PubMed PMID: .

Publications

  1. request reprint
    Uzdensky A, Derkacheva V, Dergacheva O, Zhavoronkova A. A single neuron response to photodynamic effect of various aluminum and zinc phthalocyanines. Life Sci. 2000;68:547-55 pubmed
    ..The order of these stages depended on photosensitizer type and concentration. The comparison of the dependencies of neuron lifetime on photosensitizer concentrations showed ZnPcS2 to be the most effective photosensitizer. ..
  2. request reprint
    Uzdensky A, Ma L, Iani V, Hjortland G, Steen H, Moan J. Intracellular localisation of hypericin in human glioblastoma and carcinoma cell lines. Lasers Med Sci. 2001;16:276-83 pubmed
    ..Hypericin concentrations of 10 microM or less were not toxic for WiDr cells in the dark. Orange light (lambda max approximately 600 nm; 6 mW/cm2) killed the cells stained with 1 microM hypericin with LD50 approximately 1 J/cm2. ..
  3. Fedorenko G, Fedorenko Y, Fedorenko A, Uzdensky A. Dynamics of ultrastructural alterations in photosensitized crayfish glial and neuronal cells: Structures involved in transport processes and neuroglial interactions. J Neurosci Res. 2011;89:341-51 pubmed publisher
    ....
  4. request reprint
    Uzdensky A, Bragin D, Kolosov M, Dergacheva O, Fedorenko G, Zhavoronkova A. Photodynamic inactivation of isolated crayfish mechanoreceptor neuron: different death modes under different photosensitizer concentrations. Photochem Photobiol. 2002;76:431-7 pubmed
    ..The observed electrophysiological neuron responses to photodynamic therapy may be considered as early hallmarks of different modes of forthcoming cell death. ..
  5. request reprint
    Bragin D, Kolosov M, Uzdensky A. Photodynamic inactivation of isolated crayfish neuron requires protein kinase C, PI 3-kinase and Ca2+. J Photochem Photobiol B. 2003;70:99-105 pubmed
    ..These results showed that PKC, PI 3-kinase and Ca(2+) are involved in PDT-induced neurone inactivation and following death. ..
  6. request reprint
    Uzdensky A, Juzeniene A, Ma L, Moan J. Photodynamic inhibition of enzymatic detachment of human cancer cells from a substratum. Biochim Biophys Acta. 2004;1670:1-11 pubmed
    ..Photodynamic inhibition of enzymatic cell detachment may be related to PDT-induced inhibition of tumour metastasis. ..
  7. request reprint
    Fedorenko G, Uzdensky A. Cellular structures involved in the transport processes and neuroglial interactions in the crayfish stretch receptor. J Integr Neurosci. 2009;8:433-40 pubmed
    ..The neuroglial transport could also be performed by diffusion across the intercellular space. These data indicate the significant neuroglial exchange with cellular components. ..
  8. request reprint
    Uzdensky A, Kolosov M, Bragin D, Dergacheva O, Vanzha O, Oparina L. Involvement of adenylate cyclase and tyrosine kinase signaling pathways in response of crayfish stretch receptor neuron and satellite glia cell to photodynamic treatment. Glia. 2005;49:339-48 pubmed
    ..Adenylate cyclase but not phosphotyrosine signaling pathways modulated glial apoptosis and proliferation under photooxidative stress. ..
  9. request reprint
    Uzdensky A, Kolpakova E, Juzeniene A, Juzenas P, Moan J. The effect of sub-lethal ALA-PDT on the cytoskeleton and adhesion of cultured human cancer cells. Biochim Biophys Acta. 2005;1722:43-50 pubmed
    ..Some signal transduction processes are suggested to be involved in ALA-PDT-induced changes in cytoskeleton, cell shape, and adhesion. ..
  10. request reprint
    Fedorenko G, Uzdensky A. Dynamics of ultrastructural changes in the isolated crayfish mechanoreceptor neuron under photodynamic impact. J Neurosci Res. 2008;86:1409-16 pubmed
    ..Their alteration might underlie functional shifts. These structural changes continued to develop after abolishment of neuronal activity and led to necrosis. ..

Detail Information

Publications30

  1. request reprint
    Uzdensky A, Derkacheva V, Dergacheva O, Zhavoronkova A. A single neuron response to photodynamic effect of various aluminum and zinc phthalocyanines. Life Sci. 2000;68:547-55 pubmed
    ..The order of these stages depended on photosensitizer type and concentration. The comparison of the dependencies of neuron lifetime on photosensitizer concentrations showed ZnPcS2 to be the most effective photosensitizer. ..
  2. request reprint
    Uzdensky A, Ma L, Iani V, Hjortland G, Steen H, Moan J. Intracellular localisation of hypericin in human glioblastoma and carcinoma cell lines. Lasers Med Sci. 2001;16:276-83 pubmed
    ..Hypericin concentrations of 10 microM or less were not toxic for WiDr cells in the dark. Orange light (lambda max approximately 600 nm; 6 mW/cm2) killed the cells stained with 1 microM hypericin with LD50 approximately 1 J/cm2. ..
  3. Fedorenko G, Fedorenko Y, Fedorenko A, Uzdensky A. Dynamics of ultrastructural alterations in photosensitized crayfish glial and neuronal cells: Structures involved in transport processes and neuroglial interactions. J Neurosci Res. 2011;89:341-51 pubmed publisher
    ....
  4. request reprint
    Uzdensky A, Bragin D, Kolosov M, Dergacheva O, Fedorenko G, Zhavoronkova A. Photodynamic inactivation of isolated crayfish mechanoreceptor neuron: different death modes under different photosensitizer concentrations. Photochem Photobiol. 2002;76:431-7 pubmed
    ..The observed electrophysiological neuron responses to photodynamic therapy may be considered as early hallmarks of different modes of forthcoming cell death. ..
  5. request reprint
    Bragin D, Kolosov M, Uzdensky A. Photodynamic inactivation of isolated crayfish neuron requires protein kinase C, PI 3-kinase and Ca2+. J Photochem Photobiol B. 2003;70:99-105 pubmed
    ..These results showed that PKC, PI 3-kinase and Ca(2+) are involved in PDT-induced neurone inactivation and following death. ..
  6. request reprint
    Uzdensky A, Juzeniene A, Ma L, Moan J. Photodynamic inhibition of enzymatic detachment of human cancer cells from a substratum. Biochim Biophys Acta. 2004;1670:1-11 pubmed
    ..Photodynamic inhibition of enzymatic cell detachment may be related to PDT-induced inhibition of tumour metastasis. ..
  7. request reprint
    Fedorenko G, Uzdensky A. Cellular structures involved in the transport processes and neuroglial interactions in the crayfish stretch receptor. J Integr Neurosci. 2009;8:433-40 pubmed
    ..The neuroglial transport could also be performed by diffusion across the intercellular space. These data indicate the significant neuroglial exchange with cellular components. ..
  8. request reprint
    Uzdensky A, Kolosov M, Bragin D, Dergacheva O, Vanzha O, Oparina L. Involvement of adenylate cyclase and tyrosine kinase signaling pathways in response of crayfish stretch receptor neuron and satellite glia cell to photodynamic treatment. Glia. 2005;49:339-48 pubmed
    ..Adenylate cyclase but not phosphotyrosine signaling pathways modulated glial apoptosis and proliferation under photooxidative stress. ..
  9. request reprint
    Uzdensky A, Kolpakova E, Juzeniene A, Juzenas P, Moan J. The effect of sub-lethal ALA-PDT on the cytoskeleton and adhesion of cultured human cancer cells. Biochim Biophys Acta. 2005;1722:43-50 pubmed
    ..Some signal transduction processes are suggested to be involved in ALA-PDT-induced changes in cytoskeleton, cell shape, and adhesion. ..
  10. request reprint
    Fedorenko G, Uzdensky A. Dynamics of ultrastructural changes in the isolated crayfish mechanoreceptor neuron under photodynamic impact. J Neurosci Res. 2008;86:1409-16 pubmed
    ..Their alteration might underlie functional shifts. These structural changes continued to develop after abolishment of neuronal activity and led to necrosis. ..
  11. request reprint
    Uzdensky A, Dergacheva O, Zhavoronkova A, Ivanov A, Reshetnikov A, Ponomarev G. Photodynamic effect of deuteroporphyrin IX and hematoporphyrin derivatives on single neuron. Biochem Biophys Res Commun. 2001;281:1194-9 pubmed
    ..Extinction and amphiphilicity were shown to be the most important properties determining photodynamic efficiency of the studied photosensitizers. ..
  12. Uzdensky A, Lobanov A. Elevated activity of the crayfish stretch receptor neuron increases resistance of surrounding glial cells to apoptosis induced by photodynamic treatment. Neurosci Lett. 2010;468:89-92 pubmed publisher
    ..This could be attributed to the depletion of the energetic resources, which are transferred from glia into the neuron to support its firing, or to the neurotrophic neuron-to-glia signaling. ..
  13. Fedorenko G, Uzdensky A. Ultrastructure of neuroglial contacts in crayfish stretch receptor. Cell Tissue Res. 2009;337:477-90 pubmed publisher
    ....
  14. request reprint
    Uzdensky A, Lobanov A, Bibov M, Petin Y. Involvement of Ca2+- and cyclic adenosine monophosphate-mediated signaling pathways in photodynamic injury of isolated crayfish neuron and satellite glial cells. J Neurosci Res. 2007;85:860-70 pubmed
    ..Thus, Ca(2+)- and cAMP-mediated signaling pathways were involved in photooxidative injury of neurons and glia. Their pharmacological modulation may differently change the efficacy of photodynamic injury of neurons and glial cells. ..
  15. request reprint
    Kolosov M, Uzdensky A. Crayfish mechanoreceptor neuron prevents photoinduced apoptosis of satellite glial cells. Brain Res Bull. 2006;69:495-500 pubmed
    ..Altogether the data indicate that mechanoreceptor neurons release some signaling molecules involved in the prevention of glial apoptosis. This may provide integrity of the stretch receptor organ and its resistance to injurious factors. ..
  16. request reprint
    Lobanov A, Uzdensky A. PDT-induced death of sensory neurons and glial cells in the isolated crayfish stretch receptor after proteolytic treatment. J Neurosci Res. 2005;82:866-74 pubmed
    ..The involvement of neuron-to-glia signaling interactions in this phenomenon is suggested. ..
  17. request reprint
    Uzdensky A, Juzeniene A, Kolpakova E, Hjortland G, Juzenas P, Moan J. Photosensitization with protoporphyrin IX inhibits attachment of cancer cells to a substratum. Biochem Biophys Res Commun. 2004;322:452-7 pubmed
    ..Complex processes, including changes in cellular shape and reorganization of the cytoskeleton, are suggested to participate in the observed ALA-PDT effect on the cell adhesion. ..
  18. request reprint
    Uzdensky A, Dergacheva O, Zhavoronkova A, Reshetnikov A, Ponomarev G. Photodynamic effect of novel chlorin e6 derivatives on a single nerve cell. Life Sci. 2004;74:2185-97 pubmed
    ..Photodynamic efficiency of sensitizers depended on amphiphilicity influencing their intracellular localization. ..
  19. request reprint
    Uzdensky A, Iani V, Ma L, Moan J. Photobleaching of hypericin bound to human serum albumin, cultured adenocarcinoma cells and nude mice skin. Photochem Photobiol. 2002;76:320-8 pubmed
    ..The excitation spectrum of these products resembled that of hypericin. Hypericin appears to be more photostable than most sensitizers used in PDT, including mTHPC and Photofrin. ..
  20. Lobanov A, Uzdensky A. Protection of crayfish glial cells but not neurons from photodynamic injury by nerve growth factor. J Mol Neurosci. 2009;39:308-19 pubmed publisher
    ..Thus, intercellular signaling mediated by nerve-growth-factor-like neurotrophin, receptor tyrosine kinase, and JNK may be involved in crayfish glia protection from apoptosis induced by photodynamic treatment. ..
  21. request reprint
    Uzdensky A, Bragin D, Kolosov M, Kubin A, Loew H, Moan J. Photodynamic effect of hypericin and a water-soluble derivative on isolated crayfish neuron and surrounding glial cells. J Photochem Photobiol B. 2003;72:27-33 pubmed
    ..A minor fraction of hypericin was found in the neuron perinuclear area rich in cytoplasm organelles. This suggests the potential application of Hyp and Hyp-S for visualization and selective photodynamic treatment of malignant gliomas. ..
  22. Uzdensky A, Demyanenko S, Fedorenko G, Lapteva T, Fedorenko A. Protein Profile and Morphological Alterations in Penumbra after Focal Photothrombotic Infarction in the Rat Cerebral Cortex. Mol Neurobiol. 2017;54:4172-4188 pubmed publisher
    ..These data provide the integral view on the penumbra response to photothrombotic infarct. Some of these proteins can be potential targets for ischemic stroke therapy. ..
  23. Demyanenko S, Uzdensky A. Profiling of Signaling Proteins in Penumbra After Focal Photothrombotic Infarct in the Rat Brain Cortex. Mol Neurobiol. 2017;54:6839-6856 pubmed publisher
    ..These data provide the integral view on penumbra response to photothrombotic infarct. Some of these proteins may be potential targets for antistroke therapy. ..
  24. Uzdensky A, Komandirov M, Fedorenko G, Lobanov A. Protection effect of GDNF and neurturin on photosensitized crayfish neurons and glial cells. J Mol Neurosci. 2013;49:480-90 pubmed publisher
    ..GDNF-mediated maintenance of metabolism and ultrastructure of photosensitized neurons and glial cells may be the basis of its neuro- and glia protective effects. ..
  25. Berezhnaya E, Bibov M, Komandirov M, Neginskaya M, Rudkovskii M, Uzdensky A. Involvement of MAPK, Akt/GSK-3? and AMPK/mTOR signaling pathways in protection of remote glial cells from axotomy-induced necrosis and apoptosis in the isolated crayfish stretch receptor. Mol Cell Neurosci. 2017;83:1-5 pubmed publisher
    ..This suggests the involvement of these signaling proteins in protective, antiapoptotic and antinecrotic processes in the remote satellite glia surrounding the axotomized mechanoreceptor neuron. ..
  26. Demyanenko S, Uzdensky A. The Focal-Focal Preconditioning Effect of Photothrombotic Impact on the Signaling Protein Profile in the Penumbra Surrounding the Ischemic Core Induced by Another Photothrombotic Impact. Mol Neurobiol. 2018;55:229-248 pubmed publisher
    ..Less pro-survival proteins were downregulated in the penumbra after double than single impact. ..
  27. Neginskaya M, Berezhnaya E, Uzdensky A, Abramov A. Reactive Oxygen Species Produced by a Photodynamic Effect Induced Calcium Signal in Neurons and Astrocytes. Mol Neurobiol. 2018;55:96-102 pubmed publisher
    ..This data demonstrate that lipid peroxidation induced by PDT in neurons and astrocytes leads to activation of phospholipase C that results in production of inositol 1,4,5-trisphosphate (IP3). ..
  28. Uzdensky A. Photothrombotic Stroke as a Model of Ischemic Stroke. Transl Stroke Res. 2017;: pubmed publisher
    ..This review describes the current application of the photothrombotic stroke model for the study of cellular and molecular mechanisms of stroke development and ischemic penumbra formation, as well as for the search of anti-stroke drugs...
  29. Khaitin A, Rudkovskii M, Uzdensky A. Ca2+ mediates axotomy-induced necrosis and apoptosis of satellite glial cells remote from the transection site in the isolated crayfish mechanoreceptor. Mol Cell Neurosci. 2017;88:7-15 pubmed publisher
    ..Thus, modulating different pathways that maintain calcium homeostasis, one can modulate axotomy-induced death of glial cells remote from the transection site. ..