P G Georgiev

Summary

Country: Russia

Publications

  1. Maksimenko O, Bartkuhn M, Stakhov V, Herold M, Zolotarev N, Jox T, et al. Two new insulator proteins, Pita and ZIPIC, target CP190 to chromatin. Genome Res. 2015;25:89-99 pubmed publisher
    ..These results identify two new insulator proteins and emphasize the unifying function of CP190, which can be recruited by many DNA-binding insulator proteins. ..
  2. Erokhin M, Elizar ev P, Parshikov A, Schedl P, Georgiev P, Chetverina D. Transcriptional read-through is not sufficient to induce an epigenetic switch in the silencing activity of Polycomb response elements. Proc Natl Acad Sci U S A. 2015;112:14930-5 pubmed publisher
    ..Our results indicate that other mechanisms involving adjacent DNA regulatory elements must be implicated in heritable switch of PRE activity. ..
  3. Deykin A, Tikhonov M, Kalmykov V, Korobko I, Georgiev P, Maksimenko O. Transcription termination sequences support the expression of transgene product secreted with milk. Transgenic Res. 2019;: pubmed publisher
    ..These results suggest that transcriptional terminators, similar to the HS4 insulator, can be used to improve the reporter gene expression in transgenic animals. ..
  4. Melnikova L, Elizar ev P, Erokhin M, Molodina V, Chetverina D, Kostyuchenko M, et al. The same domain of Su(Hw) is required for enhancer blocking and direct promoter repression. Sci Rep. 2019;9:5314 pubmed publisher
    ..Taken together, these results suggest that an unknown protein or protein complex binds to the C-terminal region of Su(Hw) and is responsible for the direct repression activity of Su(Hw). ..
  5. Fedotova A, Clendinen C, Bonchuk A, Mogila V, Aoki T, Georgiev P, et al. Functional dissection of the developmentally restricted BEN domain chromatin boundary factor Insensitive. Epigenetics Chromatin. 2019;12:2 pubmed publisher
    ..Our studies indicate that Insv can assemble into a multivalent DNA-binding complex and that the N-terminal Insv multimerization domain is critical for boundary function. ..
  6. Kravchuk O, Kim M, Klepikov P, Parshikov A, Georgiev P, Savitsky M. Transvection in Drosophila: trans-interaction between yellow enhancers and promoter is strongly suppressed by a cis-promoter only in certain genomic regions. Chromosoma. 2017;126:431-441 pubmed publisher
    ..As a result, we observed strong transvection in many genomic regions, suggesting that a complete cis-preference of the enhancer-promoter interactions is mainly restricted to genes in their natural loci. ..
  7. Kyrchanova O, Georgiev P. Chromatin insulators and long-distance interactions in Drosophila. FEBS Lett. 2014;588:8-14 pubmed publisher
    ..It has also become apparent that enhancer blocking is only one of several functional activities of known insulator proteins, which also contribute to the organization of chromosome architecture and the integrity of regulatory elements. ..
  8. Melnikova L, Shapovalov I, Kostyuchenko M, Georgiev P, Golovnin A. EAST affects the activity of Su(Hw) insulators by two different mechanisms in Drosophila melanogaster. Chromosoma. 2017;126:299-311 pubmed publisher
    ..These results suggest that EAST, one of the main components of the interchromatin compartment, can regulate the activity of chromatin insulators. ..
  9. Melnikova L, Kostyuchenko M, Molodina V, Parshikov A, Georgiev P, Golovnin A. Interactions between BTB domain of CP190 and two adjacent regions in Su(Hw) are required for the insulator complex formation. Chromosoma. 2018;127:59-71 pubmed publisher
    ..2. Deletion of both regions in Su(Hw) prevents its interaction with CP190. Using mutations in vivo, we found that interactions with Su(Hw) and Mod(mdg4)-67.2 are essential for recruiting of CP190 to the Su(Hw) genomic sites. ..

More Information

Publications14

  1. Zolotarev N, Fedotova A, Kyrchanova O, Bonchuk A, Penin A, Lando A, et al. Architectural proteins Pita, Zw5,and ZIPIC contain homodimerization domain and support specific long-range interactions in Drosophila. Nucleic Acids Res. 2016;44:7228-41 pubmed publisher
    ..Many of the genomic binding sites of ZIPIC, Pita and Zw5 are located at the boundaries of topologically associated domains (TADs). Thus, ZAD-containing zinc-finger proteins can be attributed to the class of architectural proteins. ..
  2. Melnikova L, Kostyuchenko M, Molodina V, Parshikov A, Georgiev P, Golovnin A. Multiple interactions are involved in a highly specific association of the Mod(mdg4)-67.2 isoform with the Su(Hw) sites in Drosophila. Open Biol. 2017;7: pubmed publisher
  3. Bonchuk A, Maksimenko O, Kyrchanova O, Ivlieva T, Mogila V, Deshpande G, et al. Functional role of dimerization and CP190 interacting domains of CTCF protein in Drosophila melanogaster. BMC Biol. 2015;13:63 pubmed publisher
    ..We also show that the phenotypic consequences of dCTCF mutations differ depending upon when and how dCTCF activity is lost. ..
  4. Fedotova A, Bonchuk A, Mogila V, Georgiev P. C2H2 Zinc Finger Proteins: The Largest but Poorly Explored Family of Higher Eukaryotic Transcription Factors. Acta Naturae. 2017;9:47-58 pubmed
    ..The reviewed data support a proposed model according to which many of the C2H2 proteins have functions that are similar to those of the CTCF in the organization of the chromatin architecture. ..
  5. Tikhonov M, Georgiev P, Maksimenko O. Competition within Introns: Splicing Wins over Polyadenylation via a General Mechanism. Acta Naturae. 2013;5:52-61 pubmed
    ..These results confirm that the transcription apparatus ignores premature polyadenylation signals for as long as they are intronic. ..