Roman S Esipov

Summary

Country: Russia

Publications

  1. ncbi request reprint Production of recombinant oxytocin through sulfitolysis of inteincontaining fusion protein
    Roman S Esipov
    Laboratory of Biotechnology, Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, Moscow, 117997 Russia
    Protein Pept Lett 19:479-84. 2012
  2. doi request reprint Antiangiogenic and antivascular effects of a recombinant tumstatin-derived peptide in a corneal neovascularization model
    Roman Esipov
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, Moscow 117997, Russia
    Biochimie 94:1368-75. 2012
  3. ncbi request reprint Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/Bl21(DE3) system yields active recombinant enzymes
    Roman S Esipov
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, Moscow 117997, Russia
    Protein Expr Purif 24:56-60. 2002
  4. ncbi request reprint Production and purification of recombinant human oxytocin overexpressed as a hybrid protein in Escherichia coli
    Roman S Esipov
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, Moscow, 117997 Russia
    Protein Pept Lett 10:404-11. 2003
  5. ncbi request reprint Production and purification of recombinant human glucagon overexpressed as intein fusion protein in Escherichia coli
    Roman S Esipov
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, 117997 Moscow, Russia
    Protein Pept Lett 13:343-7. 2006
  6. doi request reprint Production of recombinant human epidermal growth factor using Ssp dnaB mini-intein system
    Roman S Esipov
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, Moscow 117997, Russia
    Protein Expr Purif 61:1-6. 2008
  7. doi request reprint Production of thymosin alpha1 via non-enzymatic acetylation of the recombinant precursor
    Roman S Esipov
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia Email
    Biotechnol Appl Biochem 56:17-25. 2010
  8. ncbi request reprint Ribokinase from E. coli: expression, purification, and substrate specificity
    Dmitry V Chuvikovsky
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya 16 10, Moscow 117997, Russia
    Bioorg Med Chem 14:6327-32. 2006
  9. ncbi request reprint An enzymatic transglycosylation of purine bases
    Jarkko Roivainen
    Department of Pharmaceutical Chemistry, University of Kuopio, Kuopio, Finland
    Nucleosides Nucleotides Nucleic Acids 26:905-9. 2007

Collaborators

  • Jarkko Roivainen
  • Igor A Mikhailopulo
  • Seppo Lapinjoki
  • Anatoly I Miroshnikov
  • Dmitry V Chuvikovsky
  • Tatiana Elizarova
  • Larisa A Chupova
  • Tatyana I Muravyova
  • Yuri S Skoblov

Detail Information

Publications9

  1. ncbi request reprint Production of recombinant oxytocin through sulfitolysis of inteincontaining fusion protein
    Roman S Esipov
    Laboratory of Biotechnology, Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, Moscow, 117997 Russia
    Protein Pept Lett 19:479-84. 2012
    ..The target oxytocin amide was then synthesized via methyl ester intermediate. Using this approach 6 mg of recombinant oxytocin can be obtained from 1 g of biomass...
  2. doi request reprint Antiangiogenic and antivascular effects of a recombinant tumstatin-derived peptide in a corneal neovascularization model
    Roman Esipov
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, Moscow 117997, Russia
    Biochimie 94:1368-75. 2012
    ..Our results suggest that T8 may have a therapeutic potential in the treatment of ocular neovascular diseases...
  3. ncbi request reprint Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/Bl21(DE3) system yields active recombinant enzymes
    Roman S Esipov
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, Moscow 117997, Russia
    Protein Expr Purif 24:56-60. 2002
    ..The enzyme preparations were purified to homogeneity by two steps including fractional precipitation with ammonium sulfate and subsequent chromatography on Sephadex G-100 and DEAE-Sephacel...
  4. ncbi request reprint Production and purification of recombinant human oxytocin overexpressed as a hybrid protein in Escherichia coli
    Roman S Esipov
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, Moscow, 117997 Russia
    Protein Pept Lett 10:404-11. 2003
    ..The effective method for transformation of oxytocinic acid to oxytocin was worked out. The scheme suggested allowed obtaining oxytocin in high yield...
  5. ncbi request reprint Production and purification of recombinant human glucagon overexpressed as intein fusion protein in Escherichia coli
    Roman S Esipov
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, 117997 Moscow, Russia
    Protein Pept Lett 13:343-7. 2006
    ..was designed. Expression of the obtained hybrid gene in E. coli, properties of the formed hybrid protein, and conditions of its autocatalytic cleavage leading to glucagon formation were studied...
  6. doi request reprint Production of recombinant human epidermal growth factor using Ssp dnaB mini-intein system
    Roman S Esipov
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul Miklukho Maklaya 16 10, Moscow 117997, Russia
    Protein Expr Purif 61:1-6. 2008
    ..In spite of the fact that the first scheme is more common and suitable for a variety of recombinant proteins, in case of recombinant hEGF, the second scheme proved to be more productive and cost-effective...
  7. doi request reprint Production of thymosin alpha1 via non-enzymatic acetylation of the recombinant precursor
    Roman S Esipov
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia Email
    Biotechnol Appl Biochem 56:17-25. 2010
    ..The proposed method is simple and cost-effective and is suitable for large-scale production of recombinant thymosin alpha1...
  8. ncbi request reprint Ribokinase from E. coli: expression, purification, and substrate specificity
    Dmitry V Chuvikovsky
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya 16 10, Moscow 117997, Russia
    Bioorg Med Chem 14:6327-32. 2006
    ..A new radiochemical method for monitoring the formation of D-pentofuranose-5-[32P]phosphates in the presence of [gamma-32P]ATP and RK is reported...
  9. ncbi request reprint An enzymatic transglycosylation of purine bases
    Jarkko Roivainen
    Department of Pharmaceutical Chemistry, University of Kuopio, Kuopio, Finland
    Nucleosides Nucleotides Nucleic Acids 26:905-9. 2007
    ..The glycosylation of N(6)-benzoyladenine and N(2)-acetylguanine and its O(6)-derivatives is not accompanied by deacylation of bases...