Roguev A, Xu J, Krogan N. Genetic Interaction Mapping in Schizosaccharomyces pombe Using the Pombe Epistasis Mapper (PEM) System and a ROTOR HDA Colony Replicating Robot in a 1536 Array Format. Cold Spring Harb Protoc. 2018;2018:pdb.prot091975 pubmed publisher
..Detailed program parameters for each individual replication step are provided. Using this procedure, batches of 25 or more screens can be routinely performed. ..
Roguev A, Ryan C, Hartsuiker E, Krogan N. High-Throughput Quantitative Genetic Interaction Mapping in the Fission Yeast Schizosaccharomyces pombe. Cold Spring Harb Protoc. 2018;2018:pdb.top079905 pubmed publisher
Roguev A, Ryan C, Xu J, Colson I, Hartsuiker E, Krogan N. Genetic Interaction Score (S-Score) Calculation, Clustering, and Visualization of Genetic Interaction Profiles for Yeast. Cold Spring Harb Protoc. 2018;2018:pdb.prot091983 pubmed publisher
..These methods require minimal computer skills, but some familiarity with MATLAB and Linux/Unix is a plus. Finally, an outline for using clustering and visualization software for analysis of resulting data sets is provided. ..
Roguev A, Xu J, Krogan N. Transformation of Schizosaccharomyces pombe in a 96-Well Format. Cold Spring Harb Protoc. 2018;2018:pdb.prot091942 pubmed publisher
..This protocol describes chemical transformation of Schizosaccharomyces pombe with linear DNA in a 96-well format. This procedure has been successfully used for large-scale strain construction in fission yeast. ..
Roguev A, Xu J, Krogan N. DNA Preparation from Schizosaccharomyces pombe. Cold Spring Harb Protoc. 2018;2018:pdb.prot091959 pubmed publisher
..The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes. ..