Anne Skaja Robinson

Summary

Publications

  1. ncbi request reprint Overexpression of an archaeal protein in yeast: secretion bottleneck at the ER
    Jason D Smith
    Department of Chemical Engineering, University of Delaware, Newark 19716, USA
    Biotechnol Bioeng 79:713-23. 2002
  2. ncbi request reprint Maximizing recovery of native protein from aggregates by optimizing pressure treatment
    Brian G Lefebvre
    Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA
    Biotechnol Prog 20:623-9. 2004
  3. ncbi request reprint Denaturation of an extremely stable hyperthermophilic protein occurs via a dimeric intermediate
    Sara Lawrence Powers
    Department of Chemical Engineering, University of Delaware, Newark, DE 19716, USA
    Extremophiles 11:179-89. 2007
  4. pmc Pressure dissociation studies provide insight into oligomerization competence of temperature-sensitive folding mutants of P22 tailspike
    Brian G Lefebvre
    Department of Chemical Engineering, University of Delaware, Newark, DE 19716, USA
    Protein Sci 13:1538-46. 2004
  5. pmc Decreased secretion and unfolded protein response up-regulation are correlated with intracellular retention for single-chain antibody variants produced in yeast
    Ping Xu
    Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA
    Biotechnol Bioeng 104:20-9. 2009
  6. pmc PDI improves secretion of redox-inactive beta-glucosidase
    Sara Lawrence Powers
    Department of Chemical Engineering, University of Delaware, 150 Academy Street, Newark, Delaware 19716, USA
    Biotechnol Prog 23:364-9. 2007
  7. pmc A top-down approach to mechanistic biological modeling: application to the single-chain antibody folding pathway
    Scott Hildebrandt
    Department of Chemical Engineering, Department of Computer Science, University of California, Santa Barbara, California, USA
    Biophys J 95:3535-58. 2008
  8. ncbi request reprint Heterologous GPCR expression: a bottleneck to obtaining crystal structures
    Emily C McCusker
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19711, USA
    Biotechnol Prog 23:540-7. 2007
  9. pmc Three amino acids that are critical to formation and stability of the P22 tailspike trimer
    Matthew J Gage
    259 Colburn Laboratory, Department of Chemical Engineering, University of Delaware, Newark, DE 19716, USA
    Protein Sci 14:2333-43. 2005
  10. ncbi request reprint Expression and purification of milligram levels of inactive G-protein coupled receptors in E. coli
    Steven E Bane
    Department of Chemical Engineering, University of Delaware, 150 Academy Street, Newark, DE 19716, USA
    Protein Expr Purif 52:348-55. 2007

Collaborators

  • Christopher J Roberts
  • Matthew J Gage
  • Brian G Lefebvre
  • Ronald T Niebauer
  • Sara Lawrence Powers
  • Ping Xu
  • Jason D Smith
  • Steven E Bane
  • Francis J Doyle
  • Erinc Sahin
  • Emily McCusker
  • Scott Hildebrandt
  • Emily C McCusker
  • David Raden
  • Junghwa Kim
  • Brenda L Danek
  • James A Butz
  • Kenneth J Kauffman
  • Erik J Fernandez
  • Andrea Naranjo
  • Michelle L Spatara
  • Jacob L Jordan
  • Joseph A Costanzo
  • William F Weiss
  • Linda Petzold
  • Clifford R Robinson
  • Javier E Velasquez
  • Jennifer L Zak
  • Noelle K Comolli
  • Alison Wedekind
  • Benjamin C Tang
  • Eric M Pridgen
  • Prasad S Dhurjati

Detail Information

Publications22

  1. ncbi request reprint Overexpression of an archaeal protein in yeast: secretion bottleneck at the ER
    Jason D Smith
    Department of Chemical Engineering, University of Delaware, Newark 19716, USA
    Biotechnol Bioeng 79:713-23. 2002
    ..Expression at moderately elevated temperatures (between 30 and 40 degrees C) improved beta-glucosidase yields, suggesting that higher temperature expression may improve folding and secretion yields...
  2. ncbi request reprint Maximizing recovery of native protein from aggregates by optimizing pressure treatment
    Brian G Lefebvre
    Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA
    Biotechnol Prog 20:623-9. 2004
    ..Optimizing the production of structured dimer can yield the highest level of folded protein. Typical refolding additives, such as glycerol, or low-temperature incubation did not improve yields...
  3. ncbi request reprint Denaturation of an extremely stable hyperthermophilic protein occurs via a dimeric intermediate
    Sara Lawrence Powers
    Department of Chemical Engineering, University of Delaware, Newark, DE 19716, USA
    Extremophiles 11:179-89. 2007
    ..The extremely high stability is thus derived from a combination of oligomeric interactions and subunit folding...
  4. pmc Pressure dissociation studies provide insight into oligomerization competence of temperature-sensitive folding mutants of P22 tailspike
    Brian G Lefebvre
    Department of Chemical Engineering, University of Delaware, Newark, DE 19716, USA
    Protein Sci 13:1538-46. 2004
    ..Taken together, these results suggest that E196K has a primary defect in formation of the beta-helix during monomer collapse, while G244R is primarily an assembly defect...
  5. pmc Decreased secretion and unfolded protein response up-regulation are correlated with intracellular retention for single-chain antibody variants produced in yeast
    Ping Xu
    Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA
    Biotechnol Bioeng 104:20-9. 2009
    ..This suggests that the algorithm is not useful for in vivo prediction of variants, and that in vivo screens are more effective for finding variants with improved properties...
  6. pmc PDI improves secretion of redox-inactive beta-glucosidase
    Sara Lawrence Powers
    Department of Chemical Engineering, University of Delaware, 150 Academy Street, Newark, Delaware 19716, USA
    Biotechnol Prog 23:364-9. 2007
    ..These results suggest that, unexpectedly, PDI acts in a chaperone-like capacity or possibly cooperates with the cell's folding or degradation mechanisms regardless of whether the protein is redox-active...
  7. pmc A top-down approach to mechanistic biological modeling: application to the single-chain antibody folding pathway
    Scott Hildebrandt
    Department of Chemical Engineering, Department of Computer Science, University of California, Santa Barbara, California, USA
    Biophys J 95:3535-58. 2008
    ..It also prescribed modification of the guiding hypothesis to capture BiP and PDI synergy...
  8. ncbi request reprint Heterologous GPCR expression: a bottleneck to obtaining crystal structures
    Emily C McCusker
    Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19711, USA
    Biotechnol Prog 23:540-7. 2007
    ..Developing reliable expression techniques for GPCRs is still a major priority for the structural characterization of GPCRs...
  9. pmc Three amino acids that are critical to formation and stability of the P22 tailspike trimer
    Matthew J Gage
    259 Colburn Laboratory, Department of Chemical Engineering, University of Delaware, Newark, DE 19716, USA
    Protein Sci 14:2333-43. 2005
    ..We believe that the interaction between R563 and D572 is also critical following assembly of the protrimer to properly orient D572 in order to form a salt bridge with R549 during protrimer maturation...
  10. ncbi request reprint Expression and purification of milligram levels of inactive G-protein coupled receptors in E. coli
    Steven E Bane
    Department of Chemical Engineering, University of Delaware, 150 Academy Street, Newark, DE 19716, USA
    Protein Expr Purif 52:348-55. 2007
    ..Thus this protein is suitable for additional biophysical characterization and refolding studies...
  11. ncbi request reprint Protein disulfide isomerase, but not binding protein, overexpression enhances secretion of a non-disulfide-bonded protein in yeast
    Jason D Smith
    Department of Chemical Engineering, University of Delaware, 259 Colburn Laboratory, Newark, Delaware 19716, USA
    Biotechnol Bioeng 85:340-50. 2004
    ..We suggest that PDI may be acting in a chaperone-like capacity or possibly creating mixed disulfides with the beta-glucosidase's lone cysteine residue during the folding and assembly process...
  12. ncbi request reprint Analysis of unfolded protein response during single-chain antibody expression in Saccaromyces cerevisiae reveals different roles for BiP and PDI in folding
    Ping Xu
    259 Colburn Laboratory, Department of Chemical Engineering, University of Delaware, Newark, DE 19716, USA
    Metab Eng 7:269-79. 2005
    ..Free BiP is released when scFv is folded, stabilizing Ire1p, and leading to the reduced UPR...
  13. ncbi request reprint Exceptional total and functional yields of the human adenosine (A2a) receptor expressed in the yeast Saccharomyces cerevisiae
    Ronald T Niebauer
    Department of Chemical Engineering, University of Delaware, Newark, DE 19716, USA
    Protein Expr Purif 46:204-11. 2006
    ..Importantly, the amount of functionally expressed A2a-GFP per culture ( approximately 4 mg/L) is among the highest reported for any GPCR in any expression system...
  14. pmc Nonnative interactions between cysteines direct productive assembly of P22 tailspike protein
    Brenda L Danek
    Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA
    Biophys J 85:3237-47. 2003
    ..Here we demonstrate that these three cysteines are critical for efficient assembly of tailspike trimers, and that interactions between cysteine pairs lead to productive assembly of native tailspike...
  15. pmc Refolding of G protein alpha subunits from inclusion bodies expressed in Escherichia coli
    Emily McCusker
    Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
    Protein Expr Purif 58:342-55. 2008
    ..We conclude that the refolded G(ialpha) protein has a native-like secondary structure, but is predominantly in a molten globular state...
  16. ncbi request reprint Decreases in yeast expression yields of the human adenosine A2a receptor are a result of translational or post-translational events
    Ronald T Niebauer
    Department of Chemical Engineering, University of Delaware, Newark, DE 19716, USA
    Protein Expr Purif 37:134-43. 2004
    ..Taken together, these results suggest that heterologous expression of GPCRs is limited by a translational or post-translational bottleneck that is unique from expression limitations seen for soluble proteins...
  17. doi request reprint Computational design and biophysical characterization of aggregation-resistant point mutations for γD crystallin illustrate a balance of conformational stability and intrinsic aggregation propensity
    Erinc Sahin
    Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, United States
    Biochemistry 50:628-39. 2011
    ..Surprisingly, despite being the least conformationally stable, S130P was the most resistant to aggregation, indicating a significant decrease of its IAP compared to WT and M69Q...
  18. pmc C-terminal hydrophobic interactions play a critical role in oligomeric assembly of the P22 tailspike trimer
    Matthew J Gage
    Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA
    Protein Sci 12:2732-47. 2003
    ..Taken together, these results suggest that hydrophobic interactions between C-terminal regions of P22 tailspike monomers play a critical role in the assembly of the P22 tailspike trimer...
  19. ncbi request reprint Co-expression of molecular chaperones does not improve the heterologous expression of mammalian G-protein coupled receptor expression in yeast
    James A Butz
    Department of Chemical Engineering, University of Delaware, 259 Colburn Laboratory, Newark, DE 19716, USA
    Biotechnol Bioeng 84:292-304. 2003
    ..Taken together, these results indicate that an ER-folding bottleneck does not limit the expression of the mammalian receptors in yeast...
  20. pmc Dissociation of intermolecular disulfide bonds in P22 tailspike protein intermediates in the presence of SDS
    Junghwa Kim
    Department of Chemical Engineering, University of Delaware, Newark 19716, USA
    Protein Sci 15:1791-3. 2006
    ..This result suggests that when tailspike monomer is present in free solution with SDS, the partially unfolded tailspike monomer can facilitate the reduction of disulfide bonds in the tailspike oligomers...
  21. ncbi request reprint Pressure treatment of tailspike aggregates rapidly produces on-pathway folding intermediates
    Brian G Lefebvre
    Department of Chemical Engineering, 259 Colburn Laboratory, University of Delaware, Newark, Delaware 19716, USA
    Biotechnol Bioeng 82:595-604. 2003
    ..Circular dichroism and high-pressure intrinsic tryptophan fluorescence measurements support the model that a structured intermediate is formed in a rapid manner under high pressure from a pressure-sensitive aggregate population...
  22. ncbi request reprint Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae
    Kenneth J Kauffman
    Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA
    Biotechnol Prog 18:942-50. 2002
    ..Taken together, these results suggest that control of heterologous gene expression to avoid UPR activation will result in higher production levels...