Lalita Ramakrishnan

Summary

Publications

  1. doi request reprint Looking within the zebrafish to understand the tuberculous granuloma
    Lalita Ramakrishnan
    Department of Microbiology, Medicine and Immunology, University of Washington, Seattle, WA 98195, USA
    Adv Exp Med Biol 783:251-66. 2013
  2. doi request reprint Revisiting the role of the granuloma in tuberculosis
    Lalita Ramakrishnan
    Department of Microbiology, University of Washington, Seattle, Washington 98195, USA
    Nat Rev Immunol 12:352-66. 2012
  3. pmc Tuberculous granuloma induction via interaction of a bacterial secreted protein with host epithelium
    Hannah E Volkman
    Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA 98155, USA
    Science 327:466-9. 2010
  4. pmc Drug tolerance in replicating mycobacteria mediated by a macrophage-induced efflux mechanism
    Kristin N Adams
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Cell 145:39-53. 2011
  5. pmc Tumor necrosis factor signaling mediates resistance to mycobacteria by inhibiting bacterial growth and macrophage death
    Hilary Clay
    Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA 98195, USA
    Immunity 29:283-94. 2008
  6. pmc Tuberculous granuloma formation is enhanced by a mycobacterium virulence determinant
    Hannah E Volkman
    Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, Washington, USA
    PLoS Biol 2:e367. 2004
  7. doi request reprint Evaluation of the pathogenesis and treatment of Mycobacterium marinum infection in zebrafish
    Kevin Takaki
    Department of Microbiology, University of Washington, Seattle, WA, USA
    Nat Protoc 8:1114-24. 2013
  8. pmc Trafficking of superinfecting Mycobacterium organisms into established granulomas occurs in mammals and is independent of the Erp and ESX-1 mycobacterial virulence loci
    Christine L Cosma
    Departments of Microbiology, University of Washington, Seattle, WA 98195, USA
    J Infect Dis 198:1851-5. 2008
  9. doi request reprint Zebrafish and frog models of Mycobacterium marinum infection
    Christine L Cosma
    University of Washington School of Medicine, Seattle, Washington, USA
    Curr Protoc Microbiol . 2006
  10. doi request reprint Mycobacteria manipulate macrophage recruitment through coordinated use of membrane lipids
    C J Cambier
    Department of Immunology, University of Washington, Seattle, Washington 98195, USA
    Nature 505:218-22. 2014

Collaborators

Detail Information

Publications28

  1. doi request reprint Looking within the zebrafish to understand the tuberculous granuloma
    Lalita Ramakrishnan
    Department of Microbiology, Medicine and Immunology, University of Washington, Seattle, WA 98195, USA
    Adv Exp Med Biol 783:251-66. 2013
    ..An understanding of the host and bacterial pathways involved in tuberculous granuloma formation may suggest new ways to combat mycobacterial infection...
  2. doi request reprint Revisiting the role of the granuloma in tuberculosis
    Lalita Ramakrishnan
    Department of Microbiology, University of Washington, Seattle, Washington 98195, USA
    Nat Rev Immunol 12:352-66. 2012
    ..Such therapies might be effective for tuberculosis as well as for other granulomatous diseases...
  3. pmc Tuberculous granuloma induction via interaction of a bacterial secreted protein with host epithelium
    Hannah E Volkman
    Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA 98155, USA
    Science 327:466-9. 2010
    ..Disruption of MMP9 function attenuated granuloma formation and bacterial growth. Thus, interception of epithelial MMP9 production could hold promise as a host-targeting tuberculosis therapy...
  4. pmc Drug tolerance in replicating mycobacteria mediated by a macrophage-induced efflux mechanism
    Kristin N Adams
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Cell 145:39-53. 2011
    ..Efflux pump inhibitors like verapamil reduce this tolerance. Thus, the addition of this currently approved drug or more specific efflux pump inhibitors to standard antitubercular therapy should shorten the duration of curative treatment...
  5. pmc Tumor necrosis factor signaling mediates resistance to mycobacteria by inhibiting bacterial growth and macrophage death
    Hilary Clay
    Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA 98195, USA
    Immunity 29:283-94. 2008
    ..Thus, TNF is not required for tuberculous granuloma formation, but maintains granuloma integrity indirectly by restricting mycobacterial growth within macrophages and preventing their necrosis...
  6. pmc Tuberculous granuloma formation is enhanced by a mycobacterium virulence determinant
    Hannah E Volkman
    Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, Washington, USA
    PLoS Biol 2:e367. 2004
    ..This Mycobacterium-induced macrophage aggregation in turn is tightly linked to intercellular bacterial dissemination and increased bacterial numbers. Thus, mycobacteria co-opt host granulomas for their virulence...
  7. doi request reprint Evaluation of the pathogenesis and treatment of Mycobacterium marinum infection in zebrafish
    Kevin Takaki
    Department of Microbiology, University of Washington, Seattle, WA, USA
    Nat Protoc 8:1114-24. 2013
    ....
  8. pmc Trafficking of superinfecting Mycobacterium organisms into established granulomas occurs in mammals and is independent of the Erp and ESX-1 mycobacterial virulence loci
    Christine L Cosma
    Departments of Microbiology, University of Washington, Seattle, WA 98195, USA
    J Infect Dis 198:1851-5. 2008
    ..These findings suggest that homing of infected macrophages to sites of infection is a general feature of the pathogenesis of tuberculosis and has important consequences for therapeutic strategies...
  9. doi request reprint Zebrafish and frog models of Mycobacterium marinum infection
    Christine L Cosma
    University of Washington School of Medicine, Seattle, Washington, USA
    Curr Protoc Microbiol . 2006
    ..Furthermore, protocols are included that describe the infection of embryonic zebrafish and the subsequent analysis of the infection in real time using DIC and fluorescence microscopy...
  10. doi request reprint Mycobacteria manipulate macrophage recruitment through coordinated use of membrane lipids
    C J Cambier
    Department of Immunology, University of Washington, Seattle, Washington 98195, USA
    Nature 505:218-22. 2014
    ....
  11. pmc Dichotomous role of the macrophage in early Mycobacterium marinum infection of the zebrafish
    Hilary Clay
    Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA 98195, USA
    Cell Host Microbe 2:29-39. 2007
    ..We propose that residence within macrophages represents an evolutionary trade-off for pathogenic mycobacteria that slows their early growth but provides a mechanism for tissue dissemination...
  12. pmc TNF dually mediates resistance and susceptibility to mycobacteria via mitochondrial reactive oxygen species
    Francisco J Roca
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Cell 153:521-34. 2013
    ....
  13. pmc Mycobacterium marinum Erp is a virulence determinant required for cell wall integrity and intracellular survival
    Christine L Cosma
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Infect Immun 74:3125-33. 2006
    ..Consistent with these in vivo findings, erp-deficient but not RD1-deficient bacteria exhibited permeability defects in vitro, which may be responsible for their specific failure to survive in host macrophages...
  14. pmc Neutrophils exert protection in the early tuberculous granuloma by oxidative killing of mycobacteria phagocytosed from infected macrophages
    Chao Tsung Yang
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Cell Host Microbe 12:301-12. 2012
    ..Our results provide a mechanistic link to the observed patterns of neutrophils in human tuberculous granulomas and the susceptibility of humans with chronic granulomatous disease to mycobacterial infection...
  15. pmc Insights into early mycobacterial pathogenesis from the zebrafish
    Robin Lesley
    Department of Microbiology, Box 357242, 1959 NE Pacific Street, University of Washington, Seattle, WA 98195, United States
    Curr Opin Microbiol 11:277-83. 2008
    ..As these studies uncover new facets of the bacteria-host interactions in tuberculosis they raise even more questions for future investigation...
  16. pmc Mycobacterium marinum infection of adult zebrafish causes caseating granulomatous tuberculosis and is moderated by adaptive immunity
    Laura E Swaim
    Department of Microbiology, Box 357242, University of Washington, Seattle, WA 98195, USA
    Infect Immun 74:6108-17. 2006
    ..This observation suggests that the macrophage aggregation defect associated with DeltaRD1 attenuation in zebrafish embryos is ongoing during adult infection...
  17. pmc Host genotype-specific therapies can optimize the inflammatory response to mycobacterial infections
    David M Tobin
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Cell 148:434-46. 2012
    ..Together, our findings suggest that host-directed therapies tailored to patient LTA4H genotypes may counter detrimental effects of either extreme of inflammation...
  18. ncbi request reprint New models for the study of Mycobacterium-host interactions
    Tamara C Pozos
    Department of Microbiology, Box 357242, 1959 Pacific Street, University of Washington Medical School, Seattle, Washington 98195, USA
    Curr Opin Immunol 16:499-505. 2004
    ....
  19. ncbi request reprint Superinfecting mycobacteria home to established tuberculous granulomas
    Christine L Cosma
    Department of Microbiology, University of Washington School of Medicine, Box 357242, Seattle, Washington 98195, USA
    Nat Immunol 5:828-35. 2004
    ..These findings demonstrate a failure of established granulomas, concentrated foci of activated macrophages and antigen-specific immune effector cells, to eradicate newly deposited mycobacteria not previously exposed to host responses...
  20. pmc An in vivo platform for rapid high-throughput antitubercular drug discovery
    Kevin Takaki
    Department of Microbiology, University of Washington, Seattle, WA 98195, USA
    Cell Rep 2:175-84. 2012
    ..The methods developed here should be widely applicable to small-molecule screens for other infectious and noninfectious diseases...
  21. ncbi request reprint The secret lives of the pathogenic mycobacteria
    Christine L Cosma
    Department of Microbiology, University of Washington, Seattle, Washington 98195, USA
    Annu Rev Microbiol 57:641-76. 2003
    ..In addition, we highlight current controversies and unanswered questions in these areas...
  22. pmc Pseudomonas aeruginosa Type III secretion system interacts with phagocytes to modulate systemic infection of zebrafish embryos
    Mark K Brannon
    Department of Pediatrics, University of Washington, Seattle, WA, USA
    Cell Microbiol 11:755-68. 2009
    ..aeruginosa pathogenesis in conditions associated with neutropenia or impaired phagocyte function...
  23. doi request reprint Insights into tuberculosis from the zebrafish model
    Russell D Berg
    Department of Molecular and Cell Biology, University of Washington, Seattle, WA 98195, USA
    Trends Mol Med 18:689-90. 2012
    ..The development of a zebrafish model, utilizing infection with the natural fish pathogen Mycobacterium marinum (Mm), has yielded important insights into tuberculosis with immediate clinical applications...
  24. pmc The lta4h locus modulates susceptibility to mycobacterial infection in zebrafish and humans
    David M Tobin
    Department of Microbiology, University of Washington, Box 357242, Seattle, WA 98195, USA
    Cell 140:717-30. 2010
    ..Our results suggest conserved roles for balanced eicosanoid production in vertebrate resistance to mycobacterial infection...
  25. doi request reprint Comparative pathogenesis of Mycobacterium marinum and Mycobacterium tuberculosis
    David M Tobin
    Department of Microbiology, University of Washington, Seattle, WA, USA
    Cell Microbiol 10:1027-39. 2008
    ..By pairing M. marinum with the simplified immune system of zebrafish embryos, many of the defining mechanisms of mycobacterial pathogenesis can be distilled and investigated in a tractable host/pathogen pair...
  26. ncbi request reprint Real-time visualization of mycobacterium-macrophage interactions leading to initiation of granuloma formation in zebrafish embryos
    J Muse Davis
    Department of Microbiology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Immunity 17:693-702. 2002
    ..Strikingly, infection can redirect normal embryonic macrophage migration, even recruiting macrophages seemingly committed to their developmentally dictated tissue sites...
  27. pmc Antimicrobial Efflux Pumps and Mycobacterium tuberculosis Drug Tolerance: Evolutionary Considerations
    John D Szumowski
    Department of Medicine Division of Infectious Diseases, University of Washington, Seattle, WA, USA
    Curr Top Microbiol Immunol 374:81-108. 2013
    ..In this light, we discuss the potential of efflux pump inhibitors such as verapamil to shorten tuberculosis treatment by their dual inhibition of tolerance and growth. ..
  28. pmc Why is long-term therapy required to cure tuberculosis?
    Lynn E Connolly
    Department of Medicine, University of Washington, Seattle, Washington, United States of America
    PLoS Med 4:e120. 2007