Timothy R D J Radstake

Summary

Publications

  1. pmc Genome-wide association study of systemic sclerosis identifies CD247 as a new susceptibility locus
    Timothy R D J Radstake
    Department of Rheumatology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Nat Genet 42:426-9. 2010
  2. pmc The pronounced Th17 profile in systemic sclerosis (SSc) together with intracellular expression of TGFbeta and IFNgamma distinguishes SSc phenotypes
    Timothy R D J Radstake
    Department of Rheumatology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    PLoS ONE 4:e5903. 2009
  3. doi request reprint TLR2 promotes Th2/Th17 responses via TLR4 and TLR7/8 by abrogating the type I IFN amplification loop
    Mark H Wenink
    Department of Rheumatology, Radboud University Nijmegen Medical Center, Geert Grooteplein 8, 6500 HB Nijmegen, The Netherlands
    J Immunol 183:6960-70. 2009
  4. pmc Macrophage migration inhibitory factor polymorphisms do not predict therapeutic response to glucocorticoids or to tumour necrosis factor alpha-neutralising treatments in rheumatoid arthritis
    Timothy R D J Radstake
    Department of Rheumatology, Experimental Rheumatology and Advanced Therapeutics, Radboud University Nijmegen Medical Centre, Geert Grooteplein 8, PO 6525 GA, Nijmegen, The Netherlands
    Ann Rheum Dis 66:1525-30. 2007
  5. pmc Novel insights in the regulation of CCL18 secretion by monocytes and dendritic cells via cytokines, toll-like receptors and rheumatoid synovial fluid
    Antoine W T van Lieshout
    Department of Rheumatology, Radboud University Nijmegen Medical Centre, The Netherlands
    BMC Immunol 7:23. 2006
  6. ncbi request reprint The functional variant of the inhibitory Fcgamma receptor IIb (CD32B) is associated with the rate of radiologic joint damage and dendritic cell function in rheumatoid arthritis
    Timothy R D J Radstake
    Department of Rheumatology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Arthritis Rheum 54:3828-37. 2006
  7. ncbi request reprint The functional variant (Asp299gly) of toll-like receptor 4 (TLR4) influences TLR4-mediated cytokine production in rheumatoid arthritis
    Mieke F Roelofs
    Department of Rheumatology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    J Rheumatol 35:558-61. 2008
  8. doi request reprint Circulating leptin and adiponectin concentrations during tumor necrosis factor blockade in patients with active rheumatoid arthritis
    Calin Popa
    Department of Rheumatology, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands
    J Rheumatol 36:724-30. 2009
  9. pmc Increased frequency and compromised function of T regulatory cells in systemic sclerosis (SSc) is related to a diminished CD69 and TGFbeta expression
    Timothy R D J Radstake
    Department of Rheumatology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    PLoS ONE 4:e5981. 2009
  10. pmc Modulation of lipoprotein plasma concentrations during long-term anti-TNF therapy in patients with active rheumatoid arthritis
    Calin Popa
    Department of General Internal Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Ann Rheum Dis 66:1503-7. 2007

Detail Information

Publications40

  1. pmc Genome-wide association study of systemic sclerosis identifies CD247 as a new susceptibility locus
    Timothy R D J Radstake
    Department of Rheumatology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Nat Genet 42:426-9. 2010
    ..39 x 10(-9) in the combined analysis). Additionally, we confirm and firmly establish the role of the MHC (P = 2.31 x 10(-18)), IRF5 (P = 1.86 x 10(-13)) and STAT4 (P = 3.37 x 10(-9)) gene regions as SSc genetic risk factors...
  2. pmc The pronounced Th17 profile in systemic sclerosis (SSc) together with intracellular expression of TGFbeta and IFNgamma distinguishes SSc phenotypes
    Timothy R D J Radstake
    Department of Rheumatology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    PLoS ONE 4:e5903. 2009
    ..Systemic sclerosis (SSc) is an autoimmune disease where controversy on Th1/Th2 balance dominates. We investigated whether the recently discovered Th17 pattern was present in SSc...
  3. doi request reprint TLR2 promotes Th2/Th17 responses via TLR4 and TLR7/8 by abrogating the type I IFN amplification loop
    Mark H Wenink
    Department of Rheumatology, Radboud University Nijmegen Medical Center, Geert Grooteplein 8, 6500 HB Nijmegen, The Netherlands
    J Immunol 183:6960-70. 2009
    ..This shift in T cell responses was inherent to the inability of TLR2-stimulated DCs to produce IL-12p70 and was dependent on the production of IL-1 and IL-6...
  4. pmc Macrophage migration inhibitory factor polymorphisms do not predict therapeutic response to glucocorticoids or to tumour necrosis factor alpha-neutralising treatments in rheumatoid arthritis
    Timothy R D J Radstake
    Department of Rheumatology, Experimental Rheumatology and Advanced Therapeutics, Radboud University Nijmegen Medical Centre, Geert Grooteplein 8, PO 6525 GA, Nijmegen, The Netherlands
    Ann Rheum Dis 66:1525-30. 2007
    ..Therefore, the question of whether two functional variants in MIF are associated with the response to tumour necrosis factor (TNF)alpha-neutralising and GC treatments in RA was investigated...
  5. pmc Novel insights in the regulation of CCL18 secretion by monocytes and dendritic cells via cytokines, toll-like receptors and rheumatoid synovial fluid
    Antoine W T van Lieshout
    Department of Rheumatology, Radboud University Nijmegen Medical Centre, The Netherlands
    BMC Immunol 7:23. 2006
    ..In this study we provide new insights in the regulation of CCL18 secretion by monocytes and dendritic cells...
  6. ncbi request reprint The functional variant of the inhibitory Fcgamma receptor IIb (CD32B) is associated with the rate of radiologic joint damage and dendritic cell function in rheumatoid arthritis
    Timothy R D J Radstake
    Department of Rheumatology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Arthritis Rheum 54:3828-37. 2006
    ..The purpose of this study was to unravel the role of the inhibitory FcgammaRIIb in rheumatoid arthritis (RA) by studying the effect of the FCGR2B 695T>C polymorphism on susceptibility to RA, severity of the disease, and DC function...
  7. ncbi request reprint The functional variant (Asp299gly) of toll-like receptor 4 (TLR4) influences TLR4-mediated cytokine production in rheumatoid arthritis
    Mieke F Roelofs
    Department of Rheumatology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    J Rheumatol 35:558-61. 2008
    ..To investigate functional consequences of the Toll-like receptor 4 (TLR4) variant (Asp299Gly) in rheumatoid arthritis (RA)...
  8. doi request reprint Circulating leptin and adiponectin concentrations during tumor necrosis factor blockade in patients with active rheumatoid arthritis
    Calin Popa
    Department of Rheumatology, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands
    J Rheumatol 36:724-30. 2009
    ..We investigated the effects of longterm therapeutic tumor necrosis factor (TNF) blockade on adipocytokine concentrations in patients with RA...
  9. pmc Increased frequency and compromised function of T regulatory cells in systemic sclerosis (SSc) is related to a diminished CD69 and TGFbeta expression
    Timothy R D J Radstake
    Department of Rheumatology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    PLoS ONE 4:e5981. 2009
    ..Regulatory T cells (Tregs) are essential in the control of tolerance. Evidence implicates Tregs in human autoimmune conditions. Here we investigated their role in systemic sclerosis (SSc)...
  10. pmc Modulation of lipoprotein plasma concentrations during long-term anti-TNF therapy in patients with active rheumatoid arthritis
    Calin Popa
    Department of General Internal Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Ann Rheum Dis 66:1503-7. 2007
    ..In the present study, we investigated the effects of 1-year therapy with anti-TNF on the lipid profile of RA patients...
  11. pmc Circulating levels of the chemokine CCL18 but not CXCL16 are elevated and correlate with disease activity in rheumatoid arthritis
    Antoine W T van Lieshout
    Department of Rheumatology, Radboud University Nijmegen Medical Centre, The Netherlands
    Ann Rheum Dis 66:1334-8. 2007
    ..CCL18 and CXCL16 are two chemokines that facilitate T cell attraction by APC, of which a role in the pathogenesis of RA has been suggested...
  12. ncbi request reprint MIF production by dendritic cells is differentially regulated by Toll-like receptors and increased during rheumatoid arthritis
    Calin Popa
    Department of Rheumatology, Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Cytokine 36:51-6. 2006
    ..The secretion of MIF by dendritic cells is differentially regulated by TLRs. In addition, TNF-alpha, RANKL, and CD40L augment MIF production by DCs and thus play a potential role in the amplification of the inflammatory loop in RA...
  13. doi request reprint The inhibitory Fc gamma IIb receptor dampens TLR4-mediated immune responses and is selectively up-regulated on dendritic cells from rheumatoid arthritis patients with quiescent disease
    Mark H Wenink
    Department of Rheumatology, Nijmegen Centre of Molecular Life Sciences and Radboud University Nijmegen Medical Centre, Geert Grooteplein 8, Nijmegen 6500 HB, The Netherlands
    J Immunol 183:4509-20. 2009
    ..This TLR4-FcgammaRIIb interaction was shown to dependent on the PI3K and Akt pathway...
  14. ncbi request reprint Pharmacogenetics of anti-TNF treatment in patients with rheumatoid arthritis
    Marieke J H Coenen
    Radboud University, Nijmegen Medical Centre, Department of Human Genetics, Internal Mail 855, PO Box 9101, 6500 HB, Nijmegen, The Netherlands
    Pharmacogenomics 8:761-73. 2007
    ..Nevertheless, replication and large prospective studies will be needed to demonstrate the validity of the identified genetic markers before implementation into daily clinical practice...
  15. doi request reprint HMOX1 promoter polymorphism modulates the relationship between disease activity and joint damage in rheumatoid arthritis
    Frank A D T G Wagener
    Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Arthritis Rheum 58:3388-93. 2008
    ..The aim of this study was to determine whether the (GT)n-repeat length within the HMOX1 promoter region is associated with RA disease severity and radiographic joint damage...
  16. doi request reprint The functional polymorphism 844 A>G in FcαRI (CD89) does not contribute to systemic sclerosis or rheumatoid arthritis susceptibility
    Jasper C A Broen
    Department of Rheumatology, Radboud University Nijmegen Medical Centre, 6500 HB Nijmegen, The Netherlands
    J Rheumatol 38:446-9. 2011
    ..To investigate the role of the Fc(α)RI 844 A>G functional polymorphism in the genetic predisposition to rheumatoid arthritis (RA) and systemic sclerosis (SSc) susceptibility...
  17. ncbi request reprint CXCL16 is elevated in the cerebrospinal fluid versus serum and in inflammatory conditions with suspected and proved central nervous system involvement
    Linda M P le Blanc
    Department of Rheumatology, Radboud University Nijmegen Medical Centre, The Netherlands
    Neurosci Lett 397:145-8. 2006
    ..CCL18 was only elevated in serum of SLE patients. These data suggest that attraction of activated memory type T cells by CXCL16 might play an important role in the orchestration of immune responses in the central nervous system...
  18. doi request reprint Impaired dendritic cell proinflammatory cytokine production in psoriatic arthritis
    Mark H Wenink
    Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Arthritis Rheum 63:3313-22. 2011
    ..We undertook this study to determine whether an impaired acute inflammatory response by dendritic cells (DCs) might compromise the clearance of bacteria and predispose to chronic inflammation...
  19. ncbi request reprint Expression of toll-like receptors 2 and 4 in rheumatoid synovial tissue and regulation by proinflammatory cytokines interleukin-12 and interleukin-18 via interferon-gamma
    Timothy R D J Radstake
    University Medical Center Nijmegen, Nijmegen, The Netherlands
    Arthritis Rheum 50:3856-65. 2004
    ..To study the expression of Toll-like receptor 2 (TLR-2) and TLR-4 and its association with proinflammatory cytokines in synovial tissue from patients with rheumatoid arthritis (RA), osteoarthritis (OA), and healthy individuals...
  20. doi request reprint The -2518A>G promoter polymorphism in the CCL2 gene is not associated with systemic sclerosis susceptibility or phenotype: results from a multicenter study of European Caucasian patients
    Timothy R D J Radstake
    Department of Rheumatology, Radboud University, Nijmegen Medical Centre, The Netherlands
    Hum Immunol 70:130-3. 2009
    ..Our results demonstrate that the functional variant -2518A>G of CCL2 is not implicated in the susceptibility or phenotype of SSc...
  21. doi request reprint Genome-wide association analysis of anti-TNF drug response in patients with rheumatoid arthritis
    Maša Umićević Mirkov
    Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Ann Rheum Dis 72:1375-81. 2013
    ..Currently, there are no means of identifying these patients before treatment. This study was aimed at identifying genetic factors predicting anti-TNF treatment outcome in patients with RA using a genome-wide association approach...
  22. ncbi request reprint Elevated CXCL16 expression by synovial macrophages recruits memory T cells into rheumatoid joints
    Robbert van der Voort
    Nijmegen Center for Molecular Life Sciences, University Medical Center St Radboud, Nijmegen, The Netherlands
    Arthritis Rheum 52:1381-91. 2005
    ..This study was undertaken to examine the presence of CXCR6+ T cells and of CXCL16, the only ligand for CXCR6, in the joints of patients with rheumatoid arthritis (RA)...
  23. pmc Genetic variants in toll-like receptors are not associated with rheumatoid arthritis susceptibility or anti-tumour necrosis factor treatment outcome
    Marieke J H Coenen
    Department of Human Genetics, Radboud University Nijmegen Medical Centre, Institute for Genetic and Metabolic Disease IGMD, Nijmegen, The Netherlands
    PLoS ONE 5:e14326. 2010
    ..We investigated if genetic variants in TLR genes are associated with RA and response to tumour necrosis factor blocking (anti-TNF) medication...
  24. ncbi request reprint Inhibition of Toll-like receptor 4 breaks the inflammatory loop in autoimmune destructive arthritis
    Shahla Abdollahi-Roodsaz
    Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Arthritis Rheum 56:2957-67. 2007
    ..The objective of this study was to investigate the involvement of TLR-4 activation in the development and progression of autoimmune destructive arthritis...
  25. ncbi request reprint Circulating CXCL16 is not related to circulating oxLDL in patients with rheumatoid arthritis
    Antoine W T van Lieshout
    Department of Rheumatology, Radboud University Nijmegen Medical Centre and Nijmegen Centre of Molecular Life Sciences, The Netherlands
    Biochem Biophys Res Commun 355:392-7. 2007
    ..These data suggest that binding of oxLDL by soluble CXCL16 does not play a role in atherosclerosis and, although confirmation in larger studies is needed, that circulating CXCL16 is not related to the presence of CVD in patients with RA...
  26. doi request reprint Common and different genetic background for rheumatoid arthritis and coeliac disease
    Marieke J H Coenen
    Department of Human Genetics, Institute for Genetic and Metabolic Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Hum Mol Genet 18:4195-203. 2009
    ..We confirmed two known loci and identified four novel ones for shared CD-RA genetic risk. Most of the shared loci further emphasize a role for adaptive and innate immunity in these diseases...
  27. ncbi request reprint Heme oxygenase-1 promoter polymorphisms do not influence susceptibility to systemic sclerosis and its clinical phenotypes
    Lenny van Bon
    Department of Rheumatology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Clin Exp Rheumatol 31:186. 2013
    ....
  28. pmc Stimulation of TLR2 and TLR4 differentially skews the balance of T cells in a mouse model of arthritis
    Shahla Abdollahi-Roodsaz
    Rheumatology Research and Advanced Therapeutics, Department of Rheumatology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    J Clin Invest 118:205-16. 2008
    ....
  29. ncbi request reprint Correlation of rheumatoid arthritis severity with the genetic functional variants and circulating levels of macrophage migration inhibitory factor
    Timothy R D J Radstake
    Department of Rheumatology, Experimental Rheumatology and Advanced Therapeutics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Arthritis Rheum 52:3020-9. 2005
    ..To study whether genetic variants of macrophage migration inhibitory factor (MIF), the MIF -173G>C and CATT(5-8) alleles, are associated with disease severity and levels of circulating MIF in patients with rheumatoid arthritis (RA)...
  30. pmc Meta-analysis of genome-wide association studies in celiac disease and rheumatoid arthritis identifies fourteen non-HLA shared loci
    Alexandra Zhernakova
    Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands
    PLoS Genet 7:e1002004. 2011
    ....
  31. pmc Validation study of existing gene expression signatures for anti-TNF treatment in patients with rheumatoid arthritis
    Erik J M Toonen
    Department of Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    PLoS ONE 7:e33199. 2012
    ..Our results confirm that gene expression profiling prior to treatment is a useful tool to predict anti-TNF (non) response...
  32. doi request reprint The orchestra of Toll-like receptors and their potential role in frequently occurring rheumatic conditions
    Mieke F Roelofs
    Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Arthritis Rheum 58:338-48. 2008
  33. doi request reprint Skewed X chromosomal inactivation impacts T regulatory cell function in systemic sclerosis
    Jasper C A Broen
    Department of Rheumatology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Ann Rheum Dis 69:2213-6. 2010
    ..gt; To investigate the role of X chromosomal inactivation (XCI) in systemic sclerosis (SSc) and its effects on forkhead box P3 (Foxp3) expression in T regulatory cells (Tregs)...
  34. doi request reprint Deciphering the genetic background of systemic sclerosis
    Jasper C A Broen
    Department of Rheumatology, Radboud University Medical Centre, Nijmegen, The Netherlands
    Expert Rev Clin Immunol 7:449-62. 2011
    ..We discuss indicators for a genetic component present in SSc, family studies, chromosomal aberrances, the involvement of the HLA region and multiple candidate genes and, finally, genome-wide association studies...
  35. pmc Local IL-13 gene transfer prior to immune-complex arthritis inhibits chondrocyte death and matrix-metalloproteinase-mediated cartilage matrix degradation despite enhanced joint inflammation
    Karin C A M Nabbe
    Department of Experimental Rheumatology and Advanced Therapeutics, University Medical Center Nijmegen, Nijmegen, The Netherlands
    Arthritis Res Ther 7:R392-401. 2005
    ..This study demonstrates that IL-13 overexpression during ICA diminished both chondrocyte death and MMP-mediated VDIPEN expression, even though joint inflammation was enhanced...
  36. pmc Cytokine and chemokine production by human pancreatic islets upon enterovirus infection
    Barbara M Schulte
    1Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    Diabetes 61:2030-6. 2012
    ..Our data represent the first comprehensive overview of inflammatory mediators that are secreted by human islets of Langerhans upon CVB infection and may shed light on the role of enteroviruses in type 1 diabetes pathogenesis...
  37. pmc Genetics of systemic sclerosis: an update
    Jasper C A Broen
    Department of Rheumatology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Curr Rheumatol Rep 14:11-21. 2012
    ..This year has been of particular interest due to the publication of a large genome-wide association study, further investigations into gene-gene interactions, and the tendency to validate genetic results in functional models...
  38. ncbi request reprint The Toll-like receptor 4 Asp299Gly functional variant is associated with decreased rheumatoid arthritis disease susceptibility but does not influence disease severity and/or outcome
    Timothy R D J Radstake
    University Medical Center Nijmegen, Nijmegen, The Netherlands
    Arthritis Rheum 50:999-1001. 2004
  39. ncbi request reprint Novel monoclonal antibodies detect elevated levels of the chemokine CCL18/DC-CK1 in serum and body fluids in pathological conditions
    Robbert van der Voort
    Department of Tumor Immunology, Nijmegen Center for Molecular Life Scences, University Medical Center St Radboud, Nijmegen, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    J Leukoc Biol 77:739-47. 2005
    ..CCL18/DC-CK1 may therefore function as an agonist (for naive T and B cells) and as an antagonist for CCR3-expressing leukocytes such as eosinophils...
  40. ncbi request reprint Role of Fcgamma receptors IIA, IIIA, and IIIB in susceptibility to rheumatoid arthritis
    Timothy R D J Radstake
    Department of Rheumatology, University Medical Centre St Radboud, Nijmegen, The Netherlands
    J Rheumatol 30:926-33. 2003
    ....