GTPase regulator associated with the focal adhesion kinase (GRAF) transcript was down-regulated in patients with myeloid malignanciesZhen Qian
Department of Hematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, China
J Exp Clin Cancer Res 29:111. 2010
..The aim of this study was to investigate the expression level of GRAF gene in those patients with myeloid malignancies including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS) and chronic myeloid leukemia (CML)...
Aberrant methylation of the death-associated protein kinase 1 (DAPK1) CpG island in chronic myeloid leukemiaJun Qian
Department of Hematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang, Jiangsu, PR China
Eur J Haematol 82:119-23. 2009
..776, P = 0.005). In one patient, the status of DAPK1 methylation became positive on the transition from CP to AP and BC. These results suggested that DAPK1 promoter methylation might play a significant role in the progression of CML...
- Methylation of DAPK1 promoter: frequent but not an adverse prognostic factor in myelodysplastic syndrome
J Qian
Department of Hematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang, Jiangsu, China
Int J Lab Hematol 32:74-81. 2010
..05). Aberrant methylation of DAPK1 gene promoter had no influence on the prognosis of MDS despite of its increasing occurrence during disease progression...
- IDH1 and IDH2 mutation analysis in Chinese patients with acute myeloid leukemia and myelodysplastic syndrome
Jiang Lin
Laboratory Center, Affiliated People s Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, People s Republic of China
Ann Hematol 91:519-25. 2012
..177 and 0.407, respectively). In conclusion, IDH1/2 mutations are recurrent but rare molecular aberrations in Chinese AML and MDS...
- Recurrent DNMT3A R882 mutations in Chinese patients with acute myeloid leukemia and myelodysplastic syndrome
Jiang Lin
Department of Haematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang, Jiangsu, People s Republic of China
PLoS ONE 6:e26906. 2011
..We conclude that DNMT3A R882 mutations are recurrent molecular aberrations in AML and MDS, and may be an adverse prognostic event in MDS...
- Abnormal methylation of GRAF promoter Chinese patients with acute myeloid leukemia
Jun Qian
Department of Hematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang, China
Leuk Res 35:783-6. 2011
..64 vs 6.42, P=0.005). These findings suggest that the hypermethylation of GRAF promoter might be one of early events in the development of AML...
[Alteration of methylation status of death-associated protein kinase (dapk) gene promoter in patients with acute myeloid leukemia]Jun Qian
Department of Hematology, Jiangsu University Affiliated People Hospital, Zhenjiang 212002, Jiangsu Province, China
Zhongguo Shi Yan Xue Ye Xue Za Zhi 18:1390-4. 2010
..It is concluded that dapk gene hypermethylation may be a common molecular event in AML...
- Aberrant methylation of DNA-damage-inducible transcript 3 promoter is a common event in patients with myelodysplastic syndrome
Jiang Lin
Department of Hematology, Affiliated People s Hospital of Jiangsu University, 8 Dianli Road, Zhenjiang, 212002, China
Leuk Res 34:991-4. 2010
..0 months vs. 23.0 months), the difference was not statistically significant (P=0.137). These findings suggest that the hypermethylation of DDIT3 promoter might be one of early events in the development of MDS...
- [Scanning of c-kit gene mutations in acute myeloid leukemias using high-resolution melting analysis]
Dong ming Yao
Department of Hematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang 212002, China
Zhonghua Xue Ye Xue Za Zhi 32:21-4. 2011
..To detect the common mutations (D816V and N822K) of c-kit gene in acute myeloid leukemia (AML) using high-resolution melting analysis (HRM)...
- Methylation status of DDIT3 gene in chronic myeloid leukemia
Ya Li Wang
Department of Hematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang, Jiangsu, PR China
J Exp Clin Cancer Res 29:54. 2010
..The aim of this study was to investigate the methylation status of DDIT3 gene in CML patients...
- [Quantification of GRAF gene expression in patients with acute myeloid leukemia using EvaGreen real time quantitative PCR]
Zhen Qian
Department of Hematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212002 PR China
Zhonghua Yi Xue Yi Chuan Xue Za Zhi 27:290-3. 2010
..To quantify the expression level of GRAF gene in acute myeloid leukemia (AML) and analyze its clinical significance...
- Aberrant methylation of CCAAT/enhancer binding protein zeta promoter in acute myeloid leukemia
Dong ming Yao
Department of Hematology, Affiliated People s Hospital of Jiangsu University, 8 Dianli Rd, Zhenjiang, Jiangsu 212002, PR China
Leuk Res 35:957-60. 2011
..167, P=0.075). There was a significant correlation between C/EBPζ expression and C/EBPζ methylation in AML patients (R=0.606, P=0.002). Our data suggest that the aberrant methylation of C/EBPζ promoter may be involved in AML...
- Aberrant hypomethylation of SALL4 gene in patients with myelodysplastic syndrome
Jiang Lin
Laboratory Center, Affiliated People s Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, PR China
Leuk Res 37:71-5. 2013
..0 months vs. 20.0 months), the difference was not statistically significant (P=0.430). These findings suggest that hypomethylation of SALL4 promoter is a common event in MDS...
- [Quantification of the PRAME transcripts in patients with acute myeloid leukemia]
Zhao Hui Zhu
Department of Hematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang, Jiangsu, P R China
Zhonghua Yi Xue Yi Chuan Xue Za Zhi 27:149-52. 2010
..To analyze the expression level and clinical significance of the preferentially expressed antigen of melanoma (PRAME) transcripts in patients with acute myeloid leukemia (AML)...
- [Quantification of prame gene transcript in chronic myeloid leukemia]
Zhao Hui Zhu
Department of Hematology, Central Laboratory, Affiliated People Hospital, Jiangsu University, Zhenjiang 212002, Jiangsu Province, China
Zhongguo Shi Yan Xue Ye Xue Za Zhi 18:855-8. 2010
..It is concluded that the level of prame gene transcript increases in CML which associates with the progression of the disease, prame gene transcript level can be used for monitoring the disease state...
- [Expression of PML-RAR alpha fusion transcripts detection by using RQ-PCR]
Lan xiu Han
Department of Hematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang 212002, China
Zhonghua Xue Ye Xue Za Zhi 29:753-6. 2008
....
- Gene expression of helicase antigen in patients with acute and chronic myeloid leukemia
Qin Chen
Department of Hematology, Jiangsu University People Hospital, Zhenjiang, Jiangsu Province, China
Zhongguo Shi Yan Xue Ye Xue Za Zhi 19:1171-5. 2011
..7%) (p = 0.008). It is concluded that HAGE cDNA expression is relevant to specific subtypes of AML and to the progression of CML...
- Association of polymorphisms of cytosine arabinoside-metabolizing enzyme gene with therapeutic efficacy for acute myeloid leukemia
Pei Pei Xu
Department of Hematology, Zhongda Hospital and Faculty of Oncology, Medical School, Southeast University, Nanjing, Jiangsu 210009, China
Chin Med J (Engl) 125:2137-43. 2012
..In this study, we aimed to investigate whether these five SNPs are associated with the therapeutic outcomes of Ara-C-based chemotherapy regimens in patients with AML...
- Rapid and reliable detection of IDH1 R132 mutations in acute myeloid leukemia using high-resolution melting curve analysis
Jiang Lin
Department of Hematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, PR China
Clin Biochem 44:779-83. 2011
..The aim of the present study was to explore the reliability of the high-resolution melting analysis (HRMA) for the identification of IDH1 R132 mutations in AML...
- U2AF1 mutations in Chinese patients with acute myeloid leukemia and myelodysplastic syndrome
Jun Qian
Department of Haematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang, Jiangsu, People s Republic of China
PLoS ONE 7:e45760. 2012
..035). No difference in the OS was observed between MDS patients with and without U2AF1 mutations. Our data show that U2AF1 mutation is a recurrent event at a low frequency in AML and MDS...
- Rapid detection of JAK2 V617F mutation using high-resolution melting analysis with LightScanner platform
Jun Qian
Department of Hematology, Affiliated People s Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, PR China
Clin Chim Acta 411:2097-100. 2010
..High-resolution melt (HRM) curve analysis, a nongel-based, automated system, is introduced as a means of mutation scanning without the requirement of any post-PCR handling...
- Methylation status of fragile histidine triad (FHIT) gene and its clinical impact on prognosis of patients with myelodysplastic syndrome
Jiang Lin
Department of Hematology, Affiliated People s Hospital, University of Jiangsu, Zhenjiang, China
Leuk Res 32:1541-5. 2008
..0 months vs. 40.0 months, P=0.025). These results suggested that aberrant methylation of the FHIT gene might be one of molecular events involved in the disease progression of MDS and be an adverse prognostic factor in MDS...
- The associations of Janus kinase-2 (JAK2) A830G polymorphism and the treatment outcomes in patients with acute myeloid leukemia
Yuejiao Zhong
Department of Hematology, Medical School, Zhongda Hospital, Sontheast University, Nanjing, China
Leuk Lymphoma 51:1115-20. 2010
..We conclude that the AA and GG genotypes of JAK2 A830G might be important markers for therapy outcomes of patients with AML in a Chinese population...
- [Quantitative study on C/EBP zeta gene transcripts in patients with chronic myeloid leukemia using real-time quantitative PCR]
Jun Qian
Department of Hematology, Renmin Affiliated Hospital, Jiangsu University, Zhenjiang, Jiangsu, P R China
Zhonghua Yi Xue Yi Chuan Xue Za Zhi 22:628-31. 2005
..To evaluate the significance of quantitative detection of CCAAT/enhancer binding protein zeta (C/EBP zeta) transcripts in patients with chronic myeloid leukemia (CML)...
- [Quantification of PML/RAR alpha fusion gene transcripts in patients with acute promyelocytic leukemia by using real-time quantitative PCR]
Jiang Lin
Key Laboratory of Experimental Hematolgy, Affiliated Peopleos Hospital, Jiangsu University, Zhenjiang, Jiangsu, 212002 People s Republic of China
Zhonghua Yi Xue Yi Chuan Xue Za Zhi 25:319-21. 2008
..To establish and evaluate a real time quantitative PCR (RQ-PCR) method for detection and quantification the PML/RAR alpha fusion gene transcripts in patients with acute promyelocytic leukemia (APL)...
- [Analysis of risk factors for relapse of 82 patients with hematologic malignancies after allogeneic hematopoietic stem cell transplantation]
Zheng Ping Yu
Department of Hematology, Zhongda Hospital, Southeast University, Nanjing 210009, China
Zhonghua Zhong Liu Za Zhi 33:283-6. 2011
..To explore the risk factors for relapse after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and the measures of prophylaxis and treatment...
- Decreased expression of CCAAT/enhancer binding protein zeta (C/EBPzeta) in patients with different myeloid diseases
Jun Qian
The Department of Hematology, The First People s Hospital of Zhenjiang, Zhenjiang, Jiangsu 212002, PR China
Leuk Res 29:1435-41. 2005
..60 versus 14.69, P<0.0001). Significant differences were also observed between patients with CML and with AML or MDS. These results suggest that the insufficient dosage of C/EBPzeta might be involved in the development of leukemia...
- [Progress of study on the transcription factor SALL4]
Jiang Lin
Department of Hematology, Jiangsu University Affiliated People Hospital, Zhenjiang Key Laboratory of Experimental Hematology, Zhenjiang 212002, Jiangsu Province, China
Zhongguo Shi Yan Xue Ye Xue Za Zhi 19:820-3. 2011
..This review focuses on the structure and function of SALL4 gene as well as its relevance to related diseases...
- [Detection of single nucleotide polymorphisms of Jak2 gene in multiple myeloma by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry]
Yue Jiao Zhoang
Department of Hematology, Zhongda Hospital, Southeast University Clinical Medical College, Nanjing 210009, Jiangsu Province, China
Zhongguo Shi Yan Xue Ye Xue Za Zhi 17:1234-7. 2009
..In conclusion, the method based on MALDI-TOF MS and PEX technique for detecting SNP in jak2 gene is rapid, accurate and reliable method, and can be used in clinical practice...
