Laszlo G Puskas

Summary

Publications

  1. ncbi request reprint High-throughput functional genomic methods to analyze the effects of dietary lipids
    Laszlo G Puskas
    Laboratory for Functional Genomics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari krt 62 Szeged, H 6726, Hungary
    Curr Pharm Biotechnol 7:525-9. 2006
  2. doi request reprint Lipid droplet binding thalidomide analogs activate endoplasmic reticulum stress and suppress hepatocellular carcinoma in a chemically induced transgenic mouse model
    Lajos I Nagy
    Avidin Ltd, Szeged, Hungary
    Lipids Health Dis 12:175. 2013
  3. pmc Transgenic fat-1 mouse as a model to study the pathophysiology of cardiovascular, neurological and psychiatric disorders
    Undurti N Das
    Jawaharlal Nehru Technological University, Kakinada 533 003, Andhra Pradesh, India
    Lipids Health Dis 8:61. 2009
  4. ncbi request reprint Expression of genes related to oxidative/nitrosative stress in mouse hearts: effect of preconditioning and cholesterol diet
    Gabriella F Kocsis
    Department of Biochemistry, University of Szeged, Szeged, Hungary
    Med Sci Monit 16:BR32-9. 2010
  5. doi request reprint Gene and protein expression changes in response to normoxic perfusion in mouse hearts
    Nóra Faragó
    Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, H 6726, Szeged, Hungary
    J Pharmacol Toxicol Methods 57:145-54. 2008
  6. ncbi request reprint Capsaicin-sensitive sensory neurons regulate myocardial function and gene expression pattern of rat hearts: a DNA microarray study
    Agnes Zvara
    Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary
    FASEB J 20:160-2. 2006
  7. doi request reprint MicroRNA-25-dependent up-regulation of NADPH oxidase 4 (NOX4) mediates hypercholesterolemia-induced oxidative/nitrative stress and subsequent dysfunction in the heart
    Zoltán V Varga
    Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Szeged, Hungary
    J Mol Cell Cardiol 62:111-21. 2013
  8. doi request reprint Cholesterol-enriched diet inhibits cardioprotection by ATP-sensitive K+ channel activators cromakalim and diazoxide
    Csaba Csonka
    Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Szeged, Hungary
    Am J Physiol Heart Circ Physiol 306:H405-13. 2014
  9. pmc Metabolic syndrome influences cardiac gene expression pattern at the transcript level in male ZDF rats
    Márta Sárközy
    Cardiovascular Research Group, Department of Biochemistry, Faculty of Medicine, University of Szeged, Szeged, Hungary
    Cardiovasc Diabetol 12:16. 2013
  10. doi request reprint Gene and protein expression profiling of the fat-1 mouse brain
    Dalma Ménesi
    Functional Genomics Laboratory, Biological Research Center of the Hungarian Academy of Sciences, Temesvari krt 62, Szeged H 6726, Hungary
    Prostaglandins Leukot Essent Fatty Acids 80:33-42. 2009

Collaborators

Detail Information

Publications36

  1. ncbi request reprint High-throughput functional genomic methods to analyze the effects of dietary lipids
    Laszlo G Puskas
    Laboratory for Functional Genomics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari krt 62 Szeged, H 6726, Hungary
    Curr Pharm Biotechnol 7:525-9. 2006
    ..Other results will also be mentioned obtained from cholesterol and polyunsaturated fatty acid fed animals by using DNA- and protein microarrays...
  2. doi request reprint Lipid droplet binding thalidomide analogs activate endoplasmic reticulum stress and suppress hepatocellular carcinoma in a chemically induced transgenic mouse model
    Lajos I Nagy
    Avidin Ltd, Szeged, Hungary
    Lipids Health Dis 12:175. 2013
    ..Hepatocellular carcinoma (HCC) is the most frequent and aggressive primary tumor of the liver and it has limited treatment options...
  3. pmc Transgenic fat-1 mouse as a model to study the pathophysiology of cardiovascular, neurological and psychiatric disorders
    Undurti N Das
    Jawaharlal Nehru Technological University, Kakinada 533 003, Andhra Pradesh, India
    Lipids Health Dis 8:61. 2009
    ..Identifying the anti-inflammatory compounds from n-3 fatty acids that suppress low-grade systemic inflammatory conditions and understanding their mechanism(s) of action may lead to newer therapeutic strategies...
  4. ncbi request reprint Expression of genes related to oxidative/nitrosative stress in mouse hearts: effect of preconditioning and cholesterol diet
    Gabriella F Kocsis
    Department of Biochemistry, University of Szeged, Szeged, Hungary
    Med Sci Monit 16:BR32-9. 2010
    ....
  5. doi request reprint Gene and protein expression changes in response to normoxic perfusion in mouse hearts
    Nóra Faragó
    Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, H 6726, Szeged, Hungary
    J Pharmacol Toxicol Methods 57:145-54. 2008
    ..Therefore, here we investigated the time-dependent effect of normoxic, normothermic perfusion on global gene expression at mRNA and protein levels...
  6. ncbi request reprint Capsaicin-sensitive sensory neurons regulate myocardial function and gene expression pattern of rat hearts: a DNA microarray study
    Agnes Zvara
    Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary
    FASEB J 20:160-2. 2006
    ..We conclude that capsaicin-sensitive sensory nerves play a significant role in the regulation of a variety of neuronal and non-neuronal genes in the heart and possibly in other tissues as well...
  7. doi request reprint MicroRNA-25-dependent up-regulation of NADPH oxidase 4 (NOX4) mediates hypercholesterolemia-induced oxidative/nitrative stress and subsequent dysfunction in the heart
    Zoltán V Varga
    Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Szeged, Hungary
    J Mol Cell Cardiol 62:111-21. 2013
    ..We conclude that hypercholesterolemia-induced microRNA alterations play an important role in the regulation of oxidative/nitrative stress and in consequent myocardial dysfunction. ..
  8. doi request reprint Cholesterol-enriched diet inhibits cardioprotection by ATP-sensitive K+ channel activators cromakalim and diazoxide
    Csaba Csonka
    Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Szeged, Hungary
    Am J Physiol Heart Circ Physiol 306:H405-13. 2014
    ..The background mechanism may include hyperlipidemia-induced attenuation of mitoKATP function by altered energy metabolism and increased oxidative stress in the heart. ..
  9. pmc Metabolic syndrome influences cardiac gene expression pattern at the transcript level in male ZDF rats
    Márta Sárközy
    Cardiovascular Research Group, Department of Biochemistry, Faculty of Medicine, University of Szeged, Szeged, Hungary
    Cardiovasc Diabetol 12:16. 2013
    ..Therefore, we examined the possible alterations in cardiac gene expression pattern in male Zucker Diabetic Fatty (ZDF) rats, a model of metabolic syndrome...
  10. doi request reprint Gene and protein expression profiling of the fat-1 mouse brain
    Dalma Ménesi
    Functional Genomics Laboratory, Biological Research Center of the Hungarian Academy of Sciences, Temesvari krt 62, Szeged H 6726, Hungary
    Prostaglandins Leukot Essent Fatty Acids 80:33-42. 2009
    ..Based on these data, it is evident that n-3 PUFAs act to modulate specific genes and formation of their protein products and thus, bring about their various beneficial actions...
  11. ncbi request reprint New molecular methods for classification, diagnosis and therapy prediction of hematological malignancies
    Agnes Zvara
    Biological Research Center, Hungarian Academy of Sciences, Szeged, H 6726, Hungary
    Pathol Oncol Res 8:231-40. 2002
    ..The aim of this study is to summarize the new, molecular based possibilities for classification, diagnosis and prognosis of hematological malignancies, as well as to summarize the main results of these areas...
  12. ncbi request reprint Improved DOP-PCR-based representational whole-genome amplification using quantitative real-time PCR
    Liliána Z Fehér
    Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary
    Diagn Mol Pathol 15:43-8. 2006
    ..8% for the traditional DOP-PCR. Our results demonstrate that our protocol preserves the original copy number of different chromosomal regions in amplified genomic DNA than standard DOP-PCR techniques more accurately...
  13. doi request reprint GABAergic neurogliaform cells represent local sources of insulin in the cerebral cortex
    Gábor Molnár
    Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, H 6726, Hungary, Laboratory of Functional Genomics, Department of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, H 6726, Hungary, Endocrinology Unit, First Department of Internal Medicine, University of Szeged, Szeged, H 6720, Hungary, and Avidin Ltd, Szeged, H 6726, Hungary
    J Neurosci 34:1133-7. 2014
    ..Thus, neurogliaform cells might link GABAergic and insulinergic action in cortical microcircuits. ..
  14. ncbi request reprint Nutrigenomic approaches to study the effects of n-3 PUFA diet in the central nervous system
    Laszlo G Puskas
    Laboratory for Functional Genomics, Biological Research Center Hungarian Academy of Sciences, P O Box 521, Szeged, H 6701, Hungary
    Nutr Health 18:227-32. 2006
    ....
  15. ncbi request reprint A cell-microelectronic sensing technique for the screening of cytoprotective compounds
    Béla Ozsvári
    Avidin Ltd, Szeged, Szeged, Hungary
    Int J Mol Med 25:525-30. 2010
    ..The RT-CES method, when used as described in the current report, is suitable for the screening of molecular libraries to identify molecules or molecule combinations that attenuate oxidative stress-induced cell damage...
  16. pmc MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changes
    Nóra Faragó
    Functional Genomics Laboratory, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary
    Lipids Health Dis 10:173. 2011
    ..Polyunsaturated fatty acids (PUFAs) such as γ-linolenic acid (GLA), arachidonic acid (AA) and docosahexaenoic acid (DHA) have cytotoxic action on glioma cells...
  17. ncbi request reprint Aromatic sulfonamides containing a condensed piperidine moiety as potential oxidative stress-inducing anticancer agents
    Ramóna Madácsi
    AVIDIN Ltd Alsó kikötö sor 11, Szeged, H 6726, Hungary
    Med Chem 9:911-9. 2013
    ....
  18. pmc Effects of dietary omega-3 polyunsaturated fatty acids on brain gene expression
    Klara Kitajka
    Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, H 6701, Szeged, Hungary
    Proc Natl Acad Sci U S A 101:10931-6. 2004
    ....
  19. ncbi request reprint Hypercholesterolemia increases myocardial oxidative and nitrosative stress thereby leading to cardiac dysfunction in apoB-100 transgenic mice
    Tamas Csont
    Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Szeged, Hungary
    Cardiovasc Res 76:100-9. 2007
    ....
  20. ncbi request reprint Kalman filtering for disease-state estimation from microarray data
    János Z Kelemen
    Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, Szeged Temesvári krt 62, H 6726, Hungary
    Bioinformatics 22:3047-53. 2006
    ....
  21. doi request reprint Altered expression of genes for Kir ion channels in dilated cardiomyopathy
    Viktoria Szuts
    Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary
    Can J Physiol Pharmacol 91:648-56. 2013
    ..These adaptations could offer a new aspect for the explanation of the generally observed physiological and molecular alterations found in DCM...
  22. ncbi request reprint A second field metachronous Merkel cell carcinoma of the lip and the palatine tonsil confirmed by microarray-based comparative genomic hybridisation
    Judit Nagy
    Department of Oral Medicine, University of Szeged Faculty of Medicine, Szeged, Hungary
    Virchows Arch 446:278-86. 2005
    ..The findings suggest that a genetically altered field was the reason for the development of the tonsillar cancer; thus, it can be regarded pathogenetically as a second field tumour...
  23. ncbi request reprint Cloning independent site-directed mutagenesis using total RNA as template
    Zsolt B Nagy
    Hungarian Academy of Sciences, Szeged, Hungary
    Biotechniques 36:762-4, 766. 2004
  24. ncbi request reprint Development of chemically modified glass surfaces for nucleic acid, protein and small molecule microarrays
    Laszlo Hackler
    Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Temesvari krt 62, Hungary
    Mol Divers 7:25-36. 2003
    ..From HPLC and mass spectrometry measurements we concluded that the covalent binding of DNA occurs through nucleobases. The tested systems offer the capability to permanently immobilize several biomolecular species in an array format...
  25. doi request reprint Digital PCR to determine the number of transcripts from single neurons after patch-clamp recording
    Nóra Faragó
    Avidin Ltd, Szeged, Hungary
    Biotechniques 54:327-36. 2013
    ..The accuracy and sensitivity of this method were further validated using synthetic spike-in templates and by detecting genes with very low levels of expression...
  26. ncbi request reprint Toxicogenomics screening of small molecules using high-density, nanocapillary real-time PCR
    Laura Vass
    Laboratory of Functional Genomics, Biological Research Center of the Hungarian Academy of Sciences, 6726 Szeged, Hungary
    Int J Mol Med 23:65-74. 2009
    ....
  27. ncbi request reprint Recovery of cyanine-dye nucleotide triphosphates
    Laszlo G Puskas
    DNA chip Laboratory, Hungarian Academy of Sciences, Szeged, H 6701, Hungary
    Anal Biochem 305:279-81. 2002
  28. ncbi request reprint Cholesterol diet-induced hyperlipidemia influences gene expression pattern of rat hearts: a DNA microarray study
    Laszlo G Puskas
    Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary
    FEBS Lett 562:99-104. 2004
    ..Although some of these genes have been suspected to be related to cardiovascular diseases, none of the genes has been previously shown to be involved in the mechanism of the cardiac effect of hyperlipidemia...
  29. ncbi request reprint Impact of venlafaxine on gene expression profile in lymphocytes of the elderly with major depression--evolution of antidepressants and the role of the "neuro-immune" system
    Janos Kalman
    Alzheimer s Disease Research Center, Department of Psychiatry, Faculty of Medicine, University of Szeged, H 6720, Szeged, Hungary
    Neurochem Res 30:1429-38. 2005
    ..These changes in lymphocytes are thought to occur in the brain, and a "neuro-immune system" is proposed by this study...
  30. doi request reprint Synthesis of novel pyrazole-based heterocycles via a copper(II)-catalysed domino annulation
    Márió Gyuris
    Avidin Ltd, Alsó Kikötő sor 11, Szeged, H 6726, Hungary
    Org Biomol Chem 11:6320-7. 2013
    ..The present study also reveals a unique amine-isocyanate coupling promotion via copper(II)-based catalytic activation...
  31. pmc Over-expression of dopamine D2 receptor and inwardly rectifying potassium channel genes in drug-naive schizophrenic peripheral blood lymphocytes as potential diagnostic markers
    Agnes Zvara
    Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Temesvari krt 62, H 6726, Hungary
    Dis Markers 21:61-9. 2005
    ..The use of both molecular markers allows a more rapid and precise prediction of schizophrenia and might help find the optimal medication for schizophrenic patients...
  32. ncbi request reprint Gene expression profiling of human cardiac potassium and sodium channels
    Balazs Ordog
    Department of Biology, Faculty of Medicine, University of Szeged, Somogyi B 4 H 6720, Szeged, Hungary
    Int J Cardiol 111:386-93. 2006
    ..The electrophysiological properties of cardiac cells are determined by the composition of ion channels and by their absolute abundance and proportional ratio...
  33. pmc Evolutionarily conserved, growth plate zone-specific regulation of the matrilin-1 promoter: L-Sox5/Sox6 and Nfi factors bound near TATA finely tune activation by Sox9
    Andrea Nagy
    Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary
    Mol Cell Biol 31:686-99. 2011
    ..Hence, our data suggest a novel model whereby Sox and Nfi proteins bind to conserved Matn1 proximal elements and functionally interact with each other to finely tune gene expression in specific zones of the cartilage growth plate...
  34. pmc Retinoic acid and hydrocortisone strengthen the barrier function of human RPMI 2650 cells, a model for nasal epithelial permeability
    Levente Kürti
    Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvari krt 62, 6726, Szeged, Hungary
    Cytotechnology 65:395-406. 2013
    ..Based on these results human RPMI 2650 cells seem to be a suitable nasal epithelial model to test different pharmaceutical excipients and various novel formulations, such as nanoparticles for toxicity and permeability...
  35. pmc Short-term administration of omega 3 fatty acids from fish oil results in increased transthyretin transcription in old rat hippocampus
    Laszlo G Puskas
    Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, P O Box 521, H 6701, Szeged, Hungary
    Proc Natl Acad Sci U S A 100:1580-5. 2003
    ..We believe the beneficial effects of fish oil might be common to other agents, i.e., induce TTR expression, like nicotine and Ginkgo biloba extract...
  36. doi request reprint Amplification of thymosin beta 10 and AKAP13 genes in metastatic and aggressive papillary thyroid carcinomas
    Liliána Z Fehér
    Laboratory of Functional Genomics, Biological Research Centre, Hungarian Academy of Sciences, P O Box 521, Szeged, 6701, Hungary
    Pathol Oncol Res 18:449-58. 2012
    ..4% of the cases with local or with distant metastasis, respectively, while no amplification was detected in non-metastatic cases. AKAP13 and TB10 regions may represent potential new genomic markers for PTC and cancer progression...