Collagen reorganization at the tumor-stromal interface facilitates local invasionPaolo P Provenzano
Department of Pharmacology, University of Wisconsin, Madison, WI, USA
BMC Med 4:38. 2006
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Nonlinear optical microscopy and computational analysis of intrinsic signatures in breast cancerCurtis T Rueden
Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, WI 53706 USA
Conf Proc IEEE Eng Med Biol Soc 2009:4077-80. 2009
..In particular we present the latest development in our SLIM Plotter application, an open source visualization program for interactive visualization and inspection of combined spectral lifetime (SLIM) data...
- Collagen density promotes mammary tumor initiation and progression
Paolo P Provenzano
Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
BMC Med 6:11. 2008
..Importantly, regions of high breast density are associated with increased stromal collagen. Thus, the influence of the extracellular matrix on breast carcinoma development and the underlying molecular mechanisms are not understood...
- Contact guidance mediated three-dimensional cell migration is regulated by Rho/ROCK-dependent matrix reorganization
Paolo P Provenzano
Department of Pharmacology, Laboratory of Molecular Biology, University of Wisconsin Paul P Carbone Comprehensive Cancer Center, University of Wisconsin, Madison, Wisconsin 53706, USA
Biophys J 95:5374-84. 2008
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- Nonlinear optical imaging of cellular processes in breast cancer
Paolo P Provenzano
Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
Microsc Microanal 14:532-48. 2008
..Furthermore, the ability to record and monitor the intrinsic fluorescence and SHG signals provides a unique tool for researchers to understand key events in cancer progression in its natural context...
- Mammary epithelial-specific disruption of focal adhesion kinase retards tumor formation and metastasis in a transgenic mouse model of human breast cancer
Paolo P Provenzano
Department of Pharmacology, Laboratory of Molecular Biology, Madison, WI 53706, USA ppproven wisc edu
Am J Pathol 173:1551-65. 2008
..Therefore, FAK acts as a primary node in the activated signaling network in transformed motile cells and is a prime candidate for novel therapeutic interventions to treat aggressive human breast cancers...
- Aligned collagen is a prognostic signature for survival in human breast carcinoma
Matthew W Conklin
Department of Pharmacology, and the Laboratory for Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
Am J Pathol 178:1221-32. 2011
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- Shining new light on 3D cell motility and the metastatic process
Paolo P Provenzano
Laboratory for Optical and Computational Instrumentation, and Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
Trends Cell Biol 19:638-48. 2009
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- Fluorescence lifetime imaging of endogenous fluorophores in histopathology sections reveals differences between normal and tumor epithelium in carcinoma in situ of the breast
Matthew W Conklin
Department of Pharmacology, University of Wisconsin, 1525 Linden Dr, Madison, WI 53706, USA
Cell Biochem Biophys 53:145-57. 2009
..Most forms of cancer have altered metabolism and redox ratios; here we present a method that has potential for early detection of these changes, which are preserved in fixed tissue samples such as classic histopathology slides...
- The role of focal adhesion kinase in tumor initiation and progression
Paolo P Provenzano
Laboratory of Molecular Biology and Departments of Pharmacology and Biomedical Engineering, University of Wisconsin, Madison, WI, USA
Cell Adh Migr 3:347-50. 2009
..Hence, FAK, as well as the FAK-regulating/regulated signaling network, are viable candidates for cancer metastasis therapies...
- Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment
Paolo P Provenzano
Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
Clin Exp Metastasis 26:357-70. 2009
..Herein, we review recent developments of multiphoton FLIM and SLIM to study 2D and 3D cell migration, invasion into the tumor microenvironment, and metastasis...
- Nonlinear optical imaging and spectral-lifetime computational analysis of endogenous and exogenous fluorophores in breast cancer
Paolo P Provenzano
University of Wisconsin, Departments of Pharmacology and Biomedical Engineering and Laboratory for Optical and Computational Instrumentation and Paul P Carbone Comprehensive Cancer Center, Madison, Wisconsin 53706, USA
J Biomed Opt 13:031220. 2008
..Using SLIM, we present a method for imaging and differentiating signals from multiple fluorophores that can have overlapping spectra via SLIM Plotter-a computational tool for visualizing and analyzing large spectral-lifetime data sets...
- Systemic administration of IGF-I enhances healing in collagenous extracellular matrices: evaluation of loaded and unloaded ligaments
Paolo P Provenzano
Dept of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
BMC Physiol 7:2. 2007
..After 3 weeks, mechanical properties, cell and matrix morphology, and biochemical composition were examined in control and healing ligaments...
Collagen fibril morphology and organization: implications for force transmission in ligament and tendonPaolo P Provenzano
Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
Matrix Biol 25:71-84. 2006
..Based upon this information and published data, we conclude that force within these tissues is directly transferred through collagen fibrils and not through an interfibrillar coupling, such as a proteoglycan bridge...
- Intrinsic fibroblast-mediated remodeling of damaged collagenous matrices in vivo
Paolo P Provenzano
Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
Matrix Biol 23:543-55. 2005
..As such, this system provides unique insight into acute tissue damage and offers a potentially powerful model to examine fibroblast behavior...
- Collagen in connective tissue: from tendon to bone
Ray Vanderby
Orthopedic Research Laboratories, Departments of Orthopedic Surgery and Biomedical Engineering, 600 Highland Avenue, 53792, Madison, WI, USA
J Biomech 36:1523-7. 2003
- Scanning electron microscopic characterization of healing and normal rat ligament microstructure under slack and loaded conditions
Christof Hurschler
Department of Orthopedics and Rehabilitation, University of Wisconsin--Madison, Madison, Wisconsin 53792-3228, USA
Connect Tissue Res 44:59-68. 2003
..The data suggest that straightening of crimped fibers is a functionally relevant phenomenon, not only in normal but also in healing ligaments...
- Subfailure damage in ligament: a structural and cellular evaluation
Paolo P Provenzano
Department of Biomedical Engineering and Department of Orthopedic Surgery, School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA
J Appl Physiol 92:362-71. 2002
..Cellular damage was induced at ligament strains significantly below the structural damage threshold. This cellular damage is likely to be part of the natural healing process in mildly sprained ligaments...
- Hindlimb unloading alters ligament healing
Paolo P Provenzano
U Houston, TX
J Appl Physiol 94:314-24. 2003
..In addition, this study provides compelling morphological evidence explaining the altered structure-function relationship in load-deprived healing connective tissue...
- Healing of subfailure ligament injury: comparison between immature and mature ligaments in a rat model
Paolo P Provenzano
Department of Orthopaedics and Rehabilitation, University of Wisconsin-Madison, 53792-3228, USA
J Orthop Res 20:975-83. 2002
..Hence, this study, with a new injury model, showed differences in ligament healing associated with maturity and quantified the clinically observed persistance of ligament laxity...
