M Fardilha


Affiliation: University of Aveiro
Country: Portugal


  1. Matos B, Howl J, Ferreira R, Fardilha M. Exploring the effect of exercise training on testicular function. Eur J Appl Physiol. 2018;: pubmed publisher
    ..In the future, it will be important to clarify the molecular mechanisms which explain these reported discrepancies and to establish guidelines for an active lifestyle to promote healthy testicular function. ..
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    Silva J, Freitas M, Fardilha M. Phosphoprotein phosphatase 1 complexes in spermatogenesis. Curr Mol Pharmacol. 2014;7:136-46 pubmed
    ..Furthermore, current challenges on the protein phosphatases field as targets to male contraception will be addressed. ..
  3. Silva J, Freitas M, Correia B, Korrodi Gregório L, Patrício A, Pelech S, et al. Profiling signaling proteins in human spermatozoa: biomarker identification for sperm quality evaluation. Fertil Steril. 2015;104:845-856.e8 pubmed publisher
    ..This study contributed toward establishing a biomarker "fingerprint" to assess sperm quality on the basis of molecular parameters. ..
  4. Freitas M, Fardilha M. Profiling Signaling Proteins in Sertoli Cells by Co-immunoprecipitation. Methods Mol Biol. 2018;1748:73-84 pubmed publisher
    ..Moreover, we will focus on how to analyze and interpret the results of a co-immunoprecipitation before mass spectrometry analysis. ..
  5. Freitas M, Fardilha M. Sertoli Cell Preparation for Co-immunoprecipitation. Methods Mol Biol. 2018;1748:61-71 pubmed publisher
    ..In this chapter, we will focus on how to obtain a protein Sertoli cell extract suitable for co-immunoprecipitation technique. ..
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    Fardilha M, Esteves S, Korrodi Gregório L, da Cruz e Silva O, da Cruz e Silva F. The physiological relevance of protein phosphatase 1 and its interacting proteins to health and disease. Curr Med Chem. 2010;17:3996-4017 pubmed
    ..Further, it compiles 129 PP1-PIP relevant physiological interactions that are well documented in the literature. Finally, the use of PIPs as therapeutic targets will be addressed. ..
  7. Silva J, Yoon S, Domingues S, Guimarães S, Goltsev A, da Cruz E Silva E, et al. Amyloid precursor protein interaction network in human testis: sentinel proteins for male reproduction. BMC Bioinformatics. 2015;16:12 pubmed publisher
    ..Our approach allowed the identification of novel interactions and recognition of key APP interacting proteins for male reproduction, particularly in sperm-oocyte interaction. ..
  8. Fardilha M, Esteves S, Korrodi Gregório L, Pelech S, da Cruz e Silva O, da Cruz E Silva E. Protein phosphatase 1 complexes modulate sperm motility and present novel targets for male infertility. Mol Hum Reprod. 2011;17:466-77 pubmed publisher
    ..Furthermore, the potential relevance of targeting PP1-PIPs complexes to infertility diagnostics and therapeutics as well as to male contraception is also discussed. ..
  9. Felgueiras J, Silva J, Fardilha M. Adding biological meaning to human protein-protein interactions identified by yeast two-hybrid screenings: A guide through bioinformatics tools. J Proteomics. 2018;171:127-140 pubmed publisher

More Information


  1. Fardilha M, Esteves S, Korrodi Gregório L, Vintém A, Domingues S, Rebelo S, et al. Identification of the human testis protein phosphatase 1 interactome. Biochem Pharmacol. 2011;82:1403-15 pubmed publisher
    ..In fact, such diversity in PP1 regulators makes them excellent targets for pharmacological intervention. ..
  2. Silva J, Yoon S, De Bock P, Goltsev A, Gevaert K, Mendes J, et al. Construction and analysis of a human testis/sperm-enriched interaction network: Unraveling the PPP1CC2 interactome. Biochim Biophys Acta Gen Subj. 2017;1861:375-385 pubmed publisher
    ..We demonstrated for the first time the interaction between PPP1CC2 and AKAP4 in human spermatozoa and the potential of the complex as contraceptive target. ..
  3. Korrodi Gregório L, Abrantes J, Müller T, Melo Ferreira J, Marcus K, da Cruz e Silva O, et al. Not so pseudo: the evolutionary history of protein phosphatase 1 regulatory subunit 2 and related pseudogenes. BMC Evol Biol. 2013;13:242 pubmed publisher
    ..The results show that contrary to initial observations PPP1R2-related pseudogenes are not simple bystanders of the evolutionary process but may rather be at the origin of genes with novel functions. ..
  4. Korrodi Gregório L, Ferreira M, Vintém A, Wu W, Müller T, Marcus K, et al. Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa. BMC Cell Biol. 2013;14:15 pubmed publisher
    ..An intriguing possibility is that a switch in PPP1CC2 inhibitory subunits could be the trigger for sperm motility in the epididymis and/or sperm hyperactivation in the female reproductive tract. ..