Lucilia Domingues

Summary

Affiliation: University of Minho
Country: Portugal

Publications

  1. pmc Identification of candidate genes for yeast engineering to improve bioethanol production in very high gravity and lignocellulosic biomass industrial fermentations
    Francisco B Pereira
    IBB, Institute of Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biotechnol Biofuels 4:57. 2011
  2. pmc Development of a strategy to functionalize a dextrin-based hydrogel for animal cell cultures using a starch-binding module fused to RGD sequence
    Susana M Moreira
    IBB, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057, Braga, Portugal
    BMC Biotechnol 8:78. 2008
  3. pmc Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation
    Lucilia Domingues
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal
    Bioeng Bugs 1:164-71. 2010
  4. doi request reprint Plasmid-mediate transfer of FLO1 into industrial Saccharomyces cerevisiae PE-2 strain creates a strain useful for repeat-batch fermentations involving flocculation-sedimentation
    Daniel G Gomes
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Bioresour Technol 108:162-8. 2012
  5. doi request reprint Fermentation of deproteinized cheese whey powder solutions to ethanol by engineered Saccharomyces cerevisiae: effect of supplementation with corn steep liquor and repeated-batch operation with biomass recycling by flocculation
    Ana Carina Silva
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    J Ind Microbiol Biotechnol 37:973-82. 2010
  6. doi request reprint Cell recycling during repeated very high gravity bio-ethanol fermentations using the industrial Saccharomyces cerevisiae strain PE-2
    Francisco B Pereira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057, Braga, Portugal
    Biotechnol Lett 34:45-53. 2012
  7. doi request reprint Wound healing activity of the human antimicrobial peptide LL37
    Reinaldo Ramos
    IBB, Institute of Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Peptides 32:1469-76. 2011
  8. doi request reprint Robust industrial Saccharomyces cerevisiae strains for very high gravity bio-ethanol fermentations
    Francisco B Pereira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    J Biosci Bioeng 112:130-6. 2011
  9. pmc Adaptive evolution of a lactose-consuming Saccharomyces cerevisiae recombinant
    Pedro M R Guimarães
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Appl Environ Microbiol 74:1748-56. 2008
  10. ncbi request reprint Relationships between hydrodynamics and rheology of flocculating yeast suspensions in a high-cell-density airlift bioreactor
    Jaroslav Klein
    Centro de Engenharia Biólogica, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biotechnol Bioeng 89:393-9. 2005

Collaborators

Detail Information

Publications37

  1. pmc Identification of candidate genes for yeast engineering to improve bioethanol production in very high gravity and lignocellulosic biomass industrial fermentations
    Francisco B Pereira
    IBB, Institute of Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biotechnol Biofuels 4:57. 2011
    ..abstract:..
  2. pmc Development of a strategy to functionalize a dextrin-based hydrogel for animal cell cultures using a starch-binding module fused to RGD sequence
    Susana M Moreira
    IBB, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057, Braga, Portugal
    BMC Biotechnol 8:78. 2008
    ..The SBM, which belongs to an alpha-amylase from Bacillus sp. TS-23, has starch (and dextrin, depolymerized starch) affinity, acting as a binding molecule to adsorb the RGD sequence to the hydrogel surface...
  3. pmc Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation
    Lucilia Domingues
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal
    Bioeng Bugs 1:164-71. 2010
    ..Emphasis is given in the present review on strain improvement for lactose-to-ethanol bioprocesses, namely flocculent yeast strains for continuous high-cell-density systems with enhanced ethanol productivity...
  4. doi request reprint Plasmid-mediate transfer of FLO1 into industrial Saccharomyces cerevisiae PE-2 strain creates a strain useful for repeat-batch fermentations involving flocculation-sedimentation
    Daniel G Gomes
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Bioresour Technol 108:162-8. 2012
    ..86 g/L/h were achieved. Due to the flocculent nature of the recombinant strain it is possible to reduce the ethanol production cost because of lower centrifugation and distillation costs...
  5. doi request reprint Fermentation of deproteinized cheese whey powder solutions to ethanol by engineered Saccharomyces cerevisiae: effect of supplementation with corn steep liquor and repeated-batch operation with biomass recycling by flocculation
    Ana Carina Silva
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    J Ind Microbiol Biotechnol 37:973-82. 2010
    ..In addition, the easy and inexpensive recycle of the yeast biomass for repeated utilization makes this process economically attractive for industrial implementation...
  6. doi request reprint Cell recycling during repeated very high gravity bio-ethanol fermentations using the industrial Saccharomyces cerevisiae strain PE-2
    Francisco B Pereira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057, Braga, Portugal
    Biotechnol Lett 34:45-53. 2012
    ....
  7. doi request reprint Wound healing activity of the human antimicrobial peptide LL37
    Reinaldo Ramos
    IBB, Institute of Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Peptides 32:1469-76. 2011
    ..The topical application of synthetic and recombinant LL37 increased vascularization and re-epithelialization. Taken together, these results clearly demonstrate that LL37 may have a key role in wound regeneration through vascularization...
  8. doi request reprint Robust industrial Saccharomyces cerevisiae strains for very high gravity bio-ethanol fermentations
    Francisco B Pereira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    J Biosci Bioeng 112:130-6. 2011
    ....
  9. pmc Adaptive evolution of a lactose-consuming Saccharomyces cerevisiae recombinant
    Pedro M R Guimarães
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Appl Environ Microbiol 74:1748-56. 2008
    ..The evolved strain efficiently fermented threefold-concentrated cheese whey, providing an attractive alternative for the fermentation of lactose-based media...
  10. ncbi request reprint Relationships between hydrodynamics and rheology of flocculating yeast suspensions in a high-cell-density airlift bioreactor
    Jaroslav Klein
    Centro de Engenharia Biólogica, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biotechnol Bioeng 89:393-9. 2005
    ....
  11. doi request reprint Selection of Saccharomyces cerevisiae strains for efficient very high gravity bio-ethanol fermentation processes
    Francisco B Pereira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biotechnol Lett 32:1655-61. 2010
    ..cerevisiae species and that naturally adapted strains, such as PE-2 and CA1185, are likely to play a key role in facilitating the transition from laboratory technological breakthroughs to industrial-scale bio-ethanol fermentations...
  12. doi request reprint Fermentation of high concentrations of lactose to ethanol by engineered flocculent Saccharomyces cerevisiae
    Pedro M R Guimarães
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057, Braga, Portugal
    Biotechnol Lett 30:1953-8. 2008
    ..5-2 g l(-1) h(-1), in micro-aerated fermentations. The results presented here emphasise that this strain is an interesting alternative for the production of ethanol from lactose-based feedstocks...
  13. doi request reprint Comparative transcriptome analysis between original and evolved recombinant lactose-consuming Saccharomyces cerevisiae strains
    Pedro M R Guimarães
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Braga, Portugal
    Biotechnol J 3:1591-7. 2008
    ..These transcriptomic data are in agreement with some of the previously identified physiological and molecular differences between the recombinants, and point to further hypotheses to explain those differences...
  14. doi request reprint Optimization of low-cost medium for very high gravity ethanol fermentations by Saccharomyces cerevisiae using statistical experimental designs
    Francisco B Pereira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Bioresour Technol 101:7856-63. 2010
    ..6±0.5% (v/v) ethanol with a corresponding productivity of 2.4±0.1g/L/h. This study provides valuable insights into cost-effective nutritional supplementation of industrial fuel ethanol VHG fermentations...
  15. doi request reprint Nutritional requirements and strain heterogeneity in Ashbya gossypii
    Orquídea Ribeiro
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal
    J Basic Microbiol 52:582-9. 2012
    ..We demonstrate that A. gossypii utilizes ammonium as sole nitrogen source at pH 6.5, facilitating further physiological studies using chemically defined media in the future...
  16. doi request reprint Bacterial cellulose modified using recombinant proteins to improve neuronal and mesenchymal cell adhesion
    Renata Pértile
    Centre of Biological Engineering, Universidade do Minho, Braga, Portugal
    Biotechnol Prog 28:526-32. 2012
    ..The MSC neurotrophin expression in cells grown on BC membranes modified with the recombinant proteins was also analyzed...
  17. doi request reprint cDNA cloning and functional expression of the alpha-D-galactose-binding lectin frutalin in Escherichia coli
    Carla Oliveira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057, Braga, Portugal
    Mol Biotechnol 43:212-20. 2009
    ..Recombinant frutalin produced by bacteria revealed agglutination properties and carbohydrate-binding specificity similar to the native breadfruit lectin...
  18. doi request reprint Recombinant expression and purification of the antimicrobial peptide magainin-2
    Reinaldo Ramos
    Institute for Biotechnology and Bioengineering IBB, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biotechnol Prog 29:17-22. 2013
    ..Its functionality was confirmed by testing its activity against gram-negative bacteria...
  19. doi request reprint Recombinant lectins: an array of tailor-made glycan-interaction biosynthetic tools
    Carla Oliveira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal
    Crit Rev Biotechnol 33:66-80. 2013
    ..In conclusion, recombinant lectins are developing into valuable biosynthetic tools for biomedical research...
  20. pmc Cytotoxic effects of native and recombinant frutalin, a plant galactose-binding lectin, on HeLa cervical cancer cells
    Carla Oliveira
    Institute for Biotechnology and Bioengineering IBB, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    J Biomed Biotechnol 2011:568932. 2011
    ..This study provides important information about the bioactivity of frutalin and contributes to the understanding of the plant lectins cytotoxic activity...
  21. pmc A comparative study of recombinant and native frutalin binding to human prostate tissues
    Carla Oliveira
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    BMC Biotechnol 9:78. 2009
    ..Finally, the results obtained with the two lectins were compared and their potential use as prostate tumour biomarkers was discussed...
  22. doi request reprint Fermentation of lactose to bio-ethanol by yeasts as part of integrated solutions for the valorisation of cheese whey
    Pedro M R Guimarães
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biotechnol Adv 28:375-84. 2010
    ..Current developments on strain engineering together with the growing market for biofuels will likely boost the industrial interest in such processes...
  23. doi request reprint The novel Fh8 and H fusion partners for soluble protein expression in Escherichia coli: a comparison with the traditional gene fusion technology
    Sofia J Costa
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057, Portugal, Portugal
    Appl Microbiol Biotechnol 97:6779-91. 2013
    ....
  24. doi request reprint Technological trends, global market, and challenges of bio-ethanol production
    Solange I Mussatto
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal
    Biotechnol Adv 28:817-30. 2010
    ..Finally, technological trends to expand this market are discussed focusing on promising strategies like the use of microalgae and continuous systems with immobilized cells...
  25. doi request reprint Biological activity of heterologous murine interleukin-10 and preliminary studies on the use of a dextrin nanogel as a delivery system
    Vera Carvalho
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057, Braga, Portugal
    Int J Pharm 400:234-42. 2010
    ..Altogether, these results demonstrate the suitability of dextrin nanogel to be used as a system for the controlled release of IL-10...
  26. doi request reprint Recombinant microbial systems for improved β-galactosidase production and biotechnological applications
    Carla Oliveira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biotechnol Adv 29:600-9. 2011
    ..Thermostable β-galactosidases (cold-adapted or thermophilic) in addition to the growing market for functional foods will likely redouble its industrial interest...
  27. doi request reprint Improving bacterial cellulose for blood vessel replacement: Functionalization with a chimeric protein containing a cellulose-binding module and an adhesion peptide
    Fábia K Andrade
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal
    Acta Biomater 6:4034-41. 2010
    ..CBM may be combined with virtually any biologically active protein for the modification of cellulose-based materials, for in vitro or in vivo applications...
  28. pmc Stimulation of zero-trans rates of lactose and maltose uptake into yeasts by preincubation with hexose to increase the adenylate energy charge
    Pedro M R Guimarães
    VTT Technical Research Centre of Finland, POB 1000, FI 02044 VTT, Finland
    Appl Environ Microbiol 74:3076-84. 2008
    ..A short exposure to glucose allows a closer approach to the sugar/H(+) symport capacity of actively metabolizing cells...
  29. doi request reprint The Fh8 tag: a fusion partner for simple and cost-effective protein purification in Escherichia coli
    Sofia J Costa
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal Instituto Nacional de Saúde Dr Ricardo Jorge INSARJ, Porto, Portugal
    Protein Expr Purif 92:163-70. 2013
    ....
  30. doi request reprint Escherichia coli expression and purification of LL37 fused to a family III carbohydrate-binding module from Clostridium thermocellum
    Reinaldo Ramos
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Protein Expr Purif 71:1-7. 2010
    ..Only the recombinant LL37 obtained from the C-terminally fused protein (LK-CBM3-LL37) showed antibacterial activity against E. coli K12, with a MIC of 180 microg/ml...
  31. doi request reprint Expression of Trichoderma reesei cellulases CBHI and EGI in Ashbya gossypii
    Orquídea Ribeiro
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Appl Microbiol Biotechnol 87:1437-46. 2010
    ..Partial characterization of CBHI and EGI expressed in A. gossypii revealed overglycosylation when compared with the native T. reesei proteins, but the glycosylation was less extensive than on cellulases expressed in S. cerevisiae...
  32. doi request reprint Expression of frutalin, an alpha-D-galactose-binding jacalin-related lectin, in the yeast Pichia pastoris
    Carla Oliveira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Protein Expr Purif 60:188-93. 2008
    ..pastoris and also that linker cleavage might not be essential for lectin sugar specificity...
  33. doi request reprint Characterization of the Ashbya gossypii secreted N-glycome and genomic insights into its N-glycosylation pathway
    Tatiana Q Aguiar
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Carbohydr Res 381:19-27. 2013
    ..gossypii differs considerably from that by S. cerevisiae, allowing much shorter N-glycans. Genes for two putative N-glycan processing enzymes were identified, that did not have homologs in S. cerevisiae. ..
  34. ncbi request reprint Application of the Cre-loxP system for multiple gene disruption in the yeast Kluyveromyces marxianus
    Orquídea Ribeiro
    Institute of Biotechnology and Bioengineering IBB, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal
    J Biotechnol 131:20-6. 2007
    ..marxianus. Here, we report the validation of this system in K. marxianus CBS 6556, by disrupting two copies of the LAC4 gene, which encodes a beta-galactosidase activity...
  35. doi request reprint Differentiation of human pre-adipocytes by recombinant adiponectin
    Maria do Carmo Avides
    Department of Biological Engineering, IBB, Institute for Biotechnology and Bioengineering, University of Minho, Campus of Gualtar, 4710 057 Braga, Minho, Portugal
    Protein Expr Purif 59:122-6. 2008
    ..This renders adiponectin produced by baculovirus a better candidate for the treatment of type II diabetes and cardiovascular disease...
  36. ncbi request reprint Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger beta-galactosidase production
    Carla Oliveira
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    J Biosci Bioeng 103:318-24. 2007
    ..To our knowledge, this is the first study of multicopy delta-integrant stability in a continuous bioreactor operating at different dilution rates...
  37. doi request reprint The inhibitory effect of an RGD-human chitin-binding domain fusion protein on the adhesion of fibroblasts to reacetylated chitosan films
    Vera Carvalho
    IBB, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Mol Biotechnol 40:269-79. 2008
    ..We also conclude that this negative effect is fundamentally due to the human chitin-binding domain...