Research Topics
| Nuno F AzevedoSummaryAffiliation: University of Minho Country: Portugal Publications
| Collaborators
|
Detail Information
Publications
Nutrient shock and incubation atmosphere influence recovery of culturable Helicobacter pylori from waterN F Azevedo
, Universidade do Minho, 4700-057 Braga, Portugal
Appl Environ Microbiol 70:490-3. 2004..Besides highlighting and assessing the importance of several factors in the culturability of H. pylori, this paper demonstrates the potential ability to develop an optimized technique for recovery of this pathogen from water...- Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Salmonella spp. in a broad spectrum of samplesC Almeida
Centro de Engenharia Biólogica, Universidade do Minho, 4710 057 Braga, Portugal
Appl Environ Microbiol 76:4476-85. 2010.... - Survival of gastric and enterohepatic Helicobacter spp. in water: implications for transmissionN F Azevedo
Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
Appl Environ Microbiol 74:1805-11. 2008..felis, H. canadensis, H. pullorum, and H. canis, in water under several conditions and concludes that the roles of water in transmission between hosts are likely to be similar for all these species, whether enterohepatic or not... 
Time to "go large" on biofilm research: advantages of an omics approachNuno F Azevedo
IBB, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga 4710 057, Portugal
Biotechnol Lett 31:477-85. 2009..Whether such device and analytical methods are already available, particularly for complex heterotrophic biofilms is, however, very debatable...
A new model for the transmission of Helicobacter pylori: role of environmental reservoirs as gene pools to increase strain diversityN F Azevedo
Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Braga, Portugal
Crit Rev Microbiol 33:157-69. 2007..pylori...- Coccoid form of Helicobacter pylori as a morphological manifestation of cell adaptation to the environmentN F Azevedo
Centro de Engenharia Biólogica, Campus de Gualtar, Universidade do Minho, 4710 057 Braga, Portugal
Appl Environ Microbiol 73:3423-7. 2007..This strongly supports the view that transformation into the coccoid form is an active, biologically led process, switched on by the bacterium as a protection mechanism... - Computational approaches to standard-compliant biofilm data for reliable analysis and integrationAna Margarida Sousa
IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710 057 Braga, Portugal
J Integr Bioinform 9:203. 2012..org) - and a public repository on colony morphology signatures - MorphoCol (http://stardust.deb.uminho.pt/morphocol)... - Comparison between standard culture and peptide nucleic acid 16S rRNA hybridization quantification to study the influence of physico-chemical parameters on Legionella pneumophila survival in drinking water biofilmsM S Gião
Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Braga, Portugal
Biofouling 25:343-51. 2009..pneumophila. This raises public health concerns since culture methods are still considered to be the gold standard for assessing the presence of this opportunistic pathogen in water... - PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pyloriLaura Cerqueira
IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
BMC Microbiol 11:101. 2011..In this study, a new genotypic method to detect clarithromycin resistance in clinical samples, based on fluorescent in situ hybridization (FISH) using a set of peptide nucleic acid probes (PNA), is proposed... - Incorporation of natural uncultivable Legionella pneumophila into potable water biofilms provides a protective niche against chlorination stressM S Gião
IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Braga, Portugal
Biofouling 25:335-41. 2009..pneumophila from re-entering an infectious state by maintaining residual disinfectant levels through the entire DWDS network so that the resuscitation of cells via contact with amoebae is prevented... - Effect of chlorine on incorporation of Helicobacter pylori into drinking water biofilmsM S Gião
Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
Appl Environ Microbiol 76:1669-73. 2010..Coupled with data obtained using viability stains in pure culture, this result suggests that H. pylori can survive chlorination but remain undetectable by culture methods, which can be effectively replaced by PNA hybridization... - Validation of SYTO 9/propidium iodide uptake for rapid detection of viable but noncultivable Legionella pneumophilaM S Gião
Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
Microb Ecol 58:56-62. 2009..pneumophila cells in the environment... - Persistence of Helicobacter pylori in heterotrophic drinking-water biofilmsM S Gião
Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
Appl Environ Microbiol 74:5898-904. 2008.... - Shear stress, temperature, and inoculation concentration influence the adhesion of water-stressed Helicobacter pylori to stainless steel 304 and polypropyleneN F Azevedo
, Universidade do Minho, 4710-057 Braga, Portugal
Appl Environ Microbiol 72:2936-41. 2006..It will also allow a more rational selection of locations to perform molecular or plate culture analysis for the detection of H. pylori in drinking water-associated biofilms... - Adhesion of water stressed Helicobacter pylori to abiotic surfacesN F Azevedo
, Universidade do Minho, Braga, Portugal
J Appl Microbiol 101:718-24. 2006..It suggests that co-aggregation with the autochthonous heterotrophic consortia present in water is necessary for a longer survival of the pathogen in biofilms associated to drinking water systems... - Use of fluorescent in situ hybridisation for the visualisation of Helicobacter pylori in real drinking water biofilmsS M Braganra
Centro de Engenharia Biólogica, Universidade do Minho, 4700 057 Braga, Portugal
Water Sci Technol 55:387-93. 2007..The method requires, however, highly trained personnel for an accurate detection of the pathogen and will need simplification before being routinely used in standard water analysis laboratories... - Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimensN Guimaraes
IBB Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
J Clin Microbiol 45:3089-94. 2007..has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffin-embedded gastric biopsy specimens... - Discriminating multi-species populations in biofilms with peptide nucleic acid fluorescence in situ hybridization (PNA FISH)Carina Almeida
Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualta, Braga, Portugal
PLoS ONE 6:e14786. 2011..However, the lack of reliable techniques for the discrimination of each population has meant that studies focusing on multi-species biofilms are scarce and typically generate qualitative rather than quantitative data... - BiofOmics: a Web platform for the systematic and standardized collection of high-throughput biofilm dataAnália Lourenço
IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal
PLoS ONE 7:e39960. 2012..However, biofilm data is widespread across ad hoc, non-standardized individual files and, thus, data interchange among researchers, or any attempt of cross-laboratory experimentation or analysis, is hardly possible or even attempted... - Development and application of a novel peptide nucleic acid probe for the specific detection of Cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formulaC Almeida
Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, 4710 057 Braga, Portugal
Appl Environ Microbiol 75:2925-30. 2009..FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants... - Detection of Escherichia coli in biofilms from pipe samples and coupons in drinking water distribution networksT Juhna
Riga Technical University, Department of Water Engineering and Technology, 16 20 Azenes Street, Riga LV 1048, Latvia
Appl Environ Microbiol 73:7456-64. 2007.... - Establishment of a continuous model system to study Helicobacter pylori survival in potable water biofilmsN F Azevedo
Environmental Healthcare Unit, School of Biological Sciences, University of Southampton, Southampton, UK
Water Sci Technol 47:155-60. 2003..PNA probes provided an easy and quick way of performing fluorescence in situ hybridisation assays in heterogeneous biofilms...