R Sá-Leão

Summary

Affiliation: Universidade Nova de Lisboa
Country: Portugal

Publications

  1. pmc Low prevalence of pneumococcal carriage and high serotype and genotype diversity among adults over 60 years of age living in Portugal
    Sónia T Almeida
    Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    PLoS ONE 9:e90974. 2014
  2. pmc Epidemiology and population structure of serotypes 1, 5 and 7f carried by children in Portugal from 1996-2010 before introduction of the 10-valent and 13-valent pneumococcal conjugate vaccines
    Sónia T Almeida
    Laboratory of Molecular Microbiology of Human Pathogens, Universidade Nova de Lisboa, Oeiras, Portugal Laboratory of Molecular Genetics, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    PLoS ONE 8:e75442. 2013
  3. pmc Disease isolates of Streptococcus pseudopneumoniae and non-typeable S. pneumoniae presumptively identified as atypical S. pneumoniae in Spain
    Dora Rolo
    Institut d Investigacio Biomedica de Bellvitge, Hospital Universitari de Bellvitge, Microbiology Department, Universistat de Barcelona, Barcelona, Spain
    PLoS ONE 8:e57047. 2013
  4. pmc Decrease in pneumococcal co-colonization following vaccination with the seven-valent pneumococcal conjugate vaccine
    Carina Valente
    Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    PLoS ONE 7:e30235. 2012
  5. pmc Large screening of CA-MRSA among Staphylococcus aureus colonizing healthy young children living in two areas (urban and rural) of Portugal
    Débora A Tavares
    Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica ITQB, Universidade Nova de Lisboa UNL, Oeiras, Portugal
    BMC Infect Dis 10:110. 2010
  6. pmc Analysis of invasiveness of pneumococcal serotypes and clones circulating in Portugal before widespread use of conjugate vaccines reveals heterogeneous behavior of clones expressing the same serotype
    Raquel Sá-Leão
    Instituto de Tecnologia Quimica e Biologica, Rua da Quinta Grande, 6 Oeiras, 2780 156 Oeiras, Portugal
    J Clin Microbiol 49:1369-75. 2011
  7. ncbi request reprint Changes in pneumococcal serotypes and antibiotypes carried by vaccinated and unvaccinated day-care centre attendees in Portugal, a country with widespread use of the seven-valent pneumococcal conjugate vaccine
    R Sá-Leão
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    Clin Microbiol Infect 15:1002-7. 2009
  8. pmc High rates of transmission of and colonization by Streptococcus pneumoniae and Haemophilus influenzae within a day care center revealed in a longitudinal study
    Raquel Sá-Leão
    Laboratory of Molecular Genetics, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, 2780 156 Oeiras, Portugal
    J Clin Microbiol 46:225-34. 2008
  9. ncbi request reprint Identification, prevalence and population structure of non-typable Streptococcus pneumoniae in carriage samples isolated from preschoolers attending day-care centres
    Raquel Sá-Leão
    Molecular Genetics Laboratory, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa, Rua da Quinta Grande, 6, 2780 106 Oeiras, Portugal
    Microbiology 152:367-76. 2006
  10. ncbi request reprint Emergence of a serotype 1 Streptococcus pneumoniae lineage colonising healthy children in Portugal in the seven-valent conjugate vaccination era
    S Nunes
    Laboratory of Molecular Genetics, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    Clin Microbiol Infect 14:82-4. 2008

Collaborators

  • H de Lencastre
  • Nelson Frazão
  • Alexander Tomasz
  • Mario Ramirez
  • Jonas S Almeida
  • Kathrin Mühlemann
  • Alexandra S Simões
  • Sónia Nunes
  • Sónia T Almeida
  • Débora A Tavares
  • Dora Rolo
  • António Brito-Avô
  • Carina Valente
  • S Nunes
  • João A Carriço
  • Natacha G Sousa
  • M L Amorim
  • Fátima Breia
  • Sara Martins
  • Ana Morais
  • Idalina Valadares
  • Ana Cristina Santos Paulo
  • Josefina Liñares
  • Carmen Ardanuy
  • Asuncion Fenoll
  • Arnau Domenech
  • Francisco Pinto
  • Katherine Gould
  • Silvio D Brugger
  • Jason Hinds
  • Liliana Pereira
  • Peter W M Hermans
  • Hester J Bootsma
  • Maria Miragaia
  • Marc J Eleveld
  • L C Pereira
  • Rosario Mato
  • Ilda Santos-Sanches
  • Carla Simas
  • M Inês Crisóstomo
  • M Aires de Sousa
  • I Santos Sanches
  • J M Cabeda
  • J M Amorim

Detail Information

Publications18

  1. pmc Low prevalence of pneumococcal carriage and high serotype and genotype diversity among adults over 60 years of age living in Portugal
    Sónia T Almeida
    Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    PLoS ONE 9:e90974. 2014
    ..This study contributes to the establishment of a baseline that may be used to monitor how novel pneumococcal vaccines impact on colonization among the elderly. ..
  2. pmc Epidemiology and population structure of serotypes 1, 5 and 7f carried by children in Portugal from 1996-2010 before introduction of the 10-valent and 13-valent pneumococcal conjugate vaccines
    Sónia T Almeida
    Laboratory of Molecular Microbiology of Human Pathogens, Universidade Nova de Lisboa, Oeiras, Portugal Laboratory of Molecular Genetics, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    PLoS ONE 8:e75442. 2013
    ..These data will constitute an important baseline for upcoming surveillance studies aimed to establish the impact of novel PCVs targeting these serotypes in carriage. ..
  3. pmc Disease isolates of Streptococcus pseudopneumoniae and non-typeable S. pneumoniae presumptively identified as atypical S. pneumoniae in Spain
    Dora Rolo
    Institut d Investigacio Biomedica de Bellvitge, Hospital Universitari de Bellvitge, Microbiology Department, Universistat de Barcelona, Barcelona, Spain
    PLoS ONE 8:e57047. 2013
    ..pseudopneumoniae and one-fourth were other streptococci. We identified S. pseudopneumoniae and non-typeable pneumococci as cause of disease in Spain including invasive disease...
  4. pmc Decrease in pneumococcal co-colonization following vaccination with the seven-valent pneumococcal conjugate vaccine
    Carina Valente
    Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    PLoS ONE 7:e30235. 2012
    ..This might represent a novel potential benefit of conjugate vaccines...
  5. pmc Large screening of CA-MRSA among Staphylococcus aureus colonizing healthy young children living in two areas (urban and rural) of Portugal
    Débora A Tavares
    Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica ITQB, Universidade Nova de Lisboa UNL, Oeiras, Portugal
    BMC Infect Dis 10:110. 2010
    ..5%...
  6. pmc Analysis of invasiveness of pneumococcal serotypes and clones circulating in Portugal before widespread use of conjugate vaccines reveals heterogeneous behavior of clones expressing the same serotype
    Raquel Sá-Leão
    Instituto de Tecnologia Quimica e Biologica, Rua da Quinta Grande, 6 Oeiras, 2780 156 Oeiras, Portugal
    J Clin Microbiol 49:1369-75. 2011
    ..Continuous surveillance should be maintained, and current research should focus on uncovering the genetic determinants that contribute to the heterogeneity of invasive disease potential of clones sharing the same serotype...
  7. ncbi request reprint Changes in pneumococcal serotypes and antibiotypes carried by vaccinated and unvaccinated day-care centre attendees in Portugal, a country with widespread use of the seven-valent pneumococcal conjugate vaccine
    R Sá-Leão
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    Clin Microbiol Infect 15:1002-7. 2009
    ..Rates of antibiotic resistance remained unchanged due to a balance between reduction in VT and an increase in antimicrobial-resistant NVT...
  8. pmc High rates of transmission of and colonization by Streptococcus pneumoniae and Haemophilus influenzae within a day care center revealed in a longitudinal study
    Raquel Sá-Leão
    Laboratory of Molecular Genetics, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, 2780 156 Oeiras, Portugal
    J Clin Microbiol 46:225-34. 2008
    ..DCCs are units where permanent introduction of new clones occurs, and attendees, as a whole, provide a pool of hosts where the fittest clones find privileged opportunities to persist and expand...
  9. ncbi request reprint Identification, prevalence and population structure of non-typable Streptococcus pneumoniae in carriage samples isolated from preschoolers attending day-care centres
    Raquel Sá-Leão
    Molecular Genetics Laboratory, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa, Rua da Quinta Grande, 6, 2780 106 Oeiras, Portugal
    Microbiology 152:367-76. 2006
    ..These observations seem to indicate that NTPn have diverse genetic backgrounds and may have evolved as a distinct group of pneumococcal isolates...
  10. ncbi request reprint Emergence of a serotype 1 Streptococcus pneumoniae lineage colonising healthy children in Portugal in the seven-valent conjugate vaccination era
    S Nunes
    Laboratory of Molecular Genetics, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    Clin Microbiol Infect 14:82-4. 2008
    ..4% in 2003, and 3.1% in 2006. These observations paralleled the introduction and increased use of the seven-valent pneumococcal conjugate vaccine in the study group, suggesting a direct relationship between these events...
  11. pmc Properties of novel international drug-resistant pneumococcal clones identified in day-care centers of Lisbon, Portugal
    Natacha G Sousa
    Laboratorio de Genetica Molecular, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal
    J Clin Microbiol 43:4696-703. 2005
    ..We propose the inclusion of these novel international clones in the PMEN...
  12. ncbi request reprint Clonal and antibiotic resistance profiles of methicillin-resistant Staphylococcus aureus (MRSA) from a Portuguese hospital over time
    M L Amorim
    Laboratorio de Genetica Molecular, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa ITQB UNL, Oeiras, Portugal
    Microb Drug Resist 8:301-9. 2002
    ..In addition to these two MRSA clones that are the most frequent in Portuguese hospitals since the early 1990s, sporadic MRSA clones (representing 14% of the total) were identified and characterized...
  13. pmc Optochin resistance among Streptococcus pneumoniae strains colonizing healthy children in Portugal
    Sónia Nunes
    Laboratory of Molecular Genetics, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, 2780 156 Oeiras, Portugal
    J Clin Microbiol 46:321-4. 2008
    ..Novel optochin-resistant lineages were detected over time, suggesting that there was a continuous, although sporadic, emergence of optochin resistance...
  14. ncbi request reprint Low prevalence of methicillin-resistant strains among Staphylococcus aureus colonizing young and healthy members of the community in Portugal
    R Sá-Leão
    Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa, Oeiras, Portugal
    Microb Drug Resist 7:237-45. 2001
    ..The eight BORSA were all members of clones previously identified in international samples. In spite of the extremely high prevalence of MRSA in Portuguese hospitals, the carriage rate of MRSA in healthy and young individuals remains low...
  15. pmc Pilot study of the genetic diversity of the pneumococcal nasopharyngeal flora among children attending day care centers
    Raquel Sá-Leão
    Laboratory of Microbiology, The Rockefeller University, 1230 York Avenue, New York, NY, USA
    J Clin Microbiol 40:3577-85. 2002
    ..Quantitative resolution of majority and minority components of the pneumococcal NP flora will be of importance for evaluation of the impact of intervention strategies such as vaccination or introduction of new antimicrobial agents...
  16. ncbi request reprint Diversity of penicillin-nonsusceptible Streptococcus pneumoniae circulating in Iceland after the introduction of penicillin-resistant clone Spain(6B)-2
    Raquel Sá-Leão
    Laboratory of Microbiology, Rockefeller University, New York, New York 10021, USA
    J Infect Dis 186:966-75. 2002
    ..The major mechanism of dissemination of penicillin resistance in Iceland appears to be the repeated introduction of multiple lineages, followed by clonal spread...
  17. pmc Clonal evolution leading to maintenance of antibiotic resistance rates among colonizing Pneumococci in the PCV7 era in Portugal
    Alexandra S Simões
    Laboratory of Molecular Microbiology, Instituto de Tecnologia Quimica e Biologica, Rua da Quinta Grande, 6, Oeiras, 2780 156 Oeiras, Portugal
    J Clin Microbiol 49:2810-7. 2011
    ..In the era of PCVs, antimicrobial resistance remains a problem among the carried pneumococci. Continuous surveillance is warranted to evaluate serotype and clonal shifts leading to maintenance of antimicrobial resistance...
  18. pmc Highly penicillin-resistant multidrug-resistant pneumococcus-like strains colonizing children in Oeiras, Portugal: genomic characteristics and implications for surveillance
    Alexandra S Simões
    Laboratory of Molecular Genetics, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras 2780 156, Portugal
    J Clin Microbiol 48:238-46. 2010
    ..In our study, after correct identification of the penicillin-resistant true pneumococci, we found that penicillin resistance levels among pneumococci remained stable from 2003 to 2006...