Isabel Sá-Correia

Summary

Country: Portugal

Publications

  1. pmc Identification of candidate genes for yeast engineering to improve bioethanol production in very high gravity and lignocellulosic biomass industrial fermentations
    Francisco B Pereira
    IBB, Institute of Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biotechnol Biofuels 4:57. 2011
  2. doi request reprint Drug:H+ antiporters in chemical stress response in yeast
    Isabel Sá-Correia
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, 1049 001 Lisboa, Portugal
    Trends Microbiol 17:22-31. 2009
  3. doi request reprint 2D electrophoresis-based expression proteomics: a microbiologist's perspective
    Isabel Sá-Correia
    Institute for Biotechnology and Bioengineering, Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Technical University of Lisbon, Av Rovisco Pais, Lisbon, Portugal
    Expert Rev Proteomics 7:943-53. 2010
  4. pmc The YEASTRACT database: a tool for the analysis of transcription regulatory associations in Saccharomyces cerevisiae
    Miguel C Teixeira
    Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Avenida Rovisco Pais, 1049 001 Lisbon, Portugal
    Nucleic Acids Res 34:D446-51. 2006
  5. pmc Saccharomyces cerevisiae multidrug transporter Qdr2p (Yil121wp): localization and function as a quinidine resistance determinant
    Rita C Vargas
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Antimicrob Agents Chemother 48:2531-7. 2004
  6. ncbi request reprint Saccharomyces cerevisiae resistance to chlorinated phenoxyacetic acid herbicides involves Pdr1p-mediated transcriptional activation of TPO1 and PDR5 genes
    Miguel Cacho Teixeira
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Avenida Rovisco Pais, Lisbon, Portugal
    Biochem Biophys Res Commun 292:530-7. 2002
  7. ncbi request reprint Yeast adaptation to mancozeb involves the up-regulation of FLR1 under the coordinate control of Yap1, Rpn4, Pdr3, and Yrr1
    Miguel Cacho Teixeira
    IBB CEBQ, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Biochem Biophys Res Commun 367:249-55. 2008
  8. pmc Adaptation of Saccharomyces cerevisiae to the herbicide 2,4-dichlorophenoxyacetic acid, mediated by Msn2p- and Msn4p-regulated genes: important role of SPI1
    T Simoes
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Appl Environ Microbiol 69:4019-28. 2003
  9. ncbi request reprint Early transcriptional response of Saccharomyces cerevisiae to stress imposed by the herbicide 2,4-dichlorophenoxyacetic acid
    Miguel Cacho Teixeira
    Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisboa, Portugal
    FEMS Yeast Res 6:230-48. 2006
  10. doi request reprint Identification of genes required for maximal tolerance to high-glucose concentrations, as those present in industrial alcoholic fermentation media, through a chemogenomics approach
    Miguel C Teixeira
    IBB Institute for Biotechnology and Bioengineering, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisboa, Portugal
    OMICS 14:201-10. 2010

Collaborators

Detail Information

Publications73

  1. pmc Identification of candidate genes for yeast engineering to improve bioethanol production in very high gravity and lignocellulosic biomass industrial fermentations
    Francisco B Pereira
    IBB, Institute of Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biotechnol Biofuels 4:57. 2011
    ..abstract:..
  2. doi request reprint Drug:H+ antiporters in chemical stress response in yeast
    Isabel Sá-Correia
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, 1049 001 Lisboa, Portugal
    Trends Microbiol 17:22-31. 2009
    ..Finally, the extrapolation of the gathered knowledge on the MDR phenomenon in yeast to pathogenic fungi and higher eukaryotes is discussed...
  3. doi request reprint 2D electrophoresis-based expression proteomics: a microbiologist's perspective
    Isabel Sá-Correia
    Institute for Biotechnology and Bioengineering, Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Technical University of Lisbon, Av Rovisco Pais, Lisbon, Portugal
    Expert Rev Proteomics 7:943-53. 2010
    ....
  4. pmc The YEASTRACT database: a tool for the analysis of transcription regulatory associations in Saccharomyces cerevisiae
    Miguel C Teixeira
    Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Avenida Rovisco Pais, 1049 001 Lisbon, Portugal
    Nucleic Acids Res 34:D446-51. 2006
    ....
  5. pmc Saccharomyces cerevisiae multidrug transporter Qdr2p (Yil121wp): localization and function as a quinidine resistance determinant
    Rita C Vargas
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Antimicrob Agents Chemother 48:2531-7. 2004
    ..Although QDR2 transcription was not enhanced in response to quinidine, the results confirmed that Qdr2p is involved in the active export of quinidine out of the cell, thus conferring resistance to the drug...
  6. ncbi request reprint Saccharomyces cerevisiae resistance to chlorinated phenoxyacetic acid herbicides involves Pdr1p-mediated transcriptional activation of TPO1 and PDR5 genes
    Miguel Cacho Teixeira
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Avenida Rovisco Pais, Lisbon, Portugal
    Biochem Biophys Res Commun 292:530-7. 2002
    ....
  7. ncbi request reprint Yeast adaptation to mancozeb involves the up-regulation of FLR1 under the coordinate control of Yap1, Rpn4, Pdr3, and Yrr1
    Miguel Cacho Teixeira
    IBB CEBQ, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Biochem Biophys Res Commun 367:249-55. 2008
    ....
  8. pmc Adaptation of Saccharomyces cerevisiae to the herbicide 2,4-dichlorophenoxyacetic acid, mediated by Msn2p- and Msn4p-regulated genes: important role of SPI1
    T Simoes
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Appl Environ Microbiol 69:4019-28. 2003
    ..Consistent with this concept, the concentration of (14)C-labeled 2,4-D in 2,4-D-energized adapted Deltaspi1 mutant cells and the consequent intracellular acidification are higher than in wild-type cells...
  9. ncbi request reprint Early transcriptional response of Saccharomyces cerevisiae to stress imposed by the herbicide 2,4-dichlorophenoxyacetic acid
    Miguel Cacho Teixeira
    Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisboa, Portugal
    FEMS Yeast Res 6:230-48. 2006
    ..The increased expression of genes involved in alternative carbon and nitrogen source metabolism, fatty acid beta-oxidation and autophagy was also registered, suggesting that acute herbicide stress leads to nutrient limitation...
  10. doi request reprint Identification of genes required for maximal tolerance to high-glucose concentrations, as those present in industrial alcoholic fermentation media, through a chemogenomics approach
    Miguel C Teixeira
    IBB Institute for Biotechnology and Bioengineering, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisboa, Portugal
    OMICS 14:201-10. 2010
    ....
  11. ncbi request reprint Identification and physical organization of the gene cluster involved in the biosynthesis of Burkholderia cepacia complex exopolysaccharide
    Leonilde M Moreira
    Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Biochem Biophys Res Commun 312:323-33. 2003
    ....
  12. doi request reprint Yeast response and tolerance to polyamine toxicity involving the drug : H+ antiporter Qdr3 and the transcription factors Yap1 and Gcn4
    Miguel C Teixeira
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, 1049 001 Lisboa, Portugal
    Microbiology 157:945-56. 2011
    ....
  13. ncbi request reprint MUSA: a parameter free algorithm for the identification of biologically significant motifs
    Nuno D Mendes
    INESC ID, Instituto Superior Tecnico, Rua Alves Redol 9 1000 029 Lisboa, Portugal
    Bioinformatics 22:2996-3002. 2006
    ..However, one major problem with these methods is the large number of parameters that need to be specified...
  14. pmc Sphingomonas paucimobilis beta-glucosidase Bgl1: a member of a new bacterial subfamily in glycoside hydrolase family 1
    Ana Rita Marques
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Biochem J 370:793-804. 2003
    ....
  15. doi request reprint TFRank: network-based prioritization of regulatory associations underlying transcriptional responses
    Joana P Gonçalves
    Knowledge Discovery and Bioinformatics Group, INESC ID, Portugal
    Bioinformatics 27:3149-57. 2011
    ..A paradigm shift is thus emerging, where isolated and constrained analyses will likely be replaced by whole-network, systemic-aware strategies...
  16. pmc Quantitative- and phospho-proteomic analysis of the yeast response to the tyrosine kinase inhibitor imatinib to pharmacoproteomics-guided drug line extension
    Sandra C dos Santos
    Institute for Biotechnology and Bioengineering IBB, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Technical University of Lisbon, Lisbon, Portugal
    OMICS 16:537-51. 2012
    ....
  17. ncbi request reprint The yeast multidrug transporter Qdr3 (Ybr043c): localization and role as a determinant of resistance to quinidine, barban, cisplatin, and bleomycin
    Sandra Tenreiro
    Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Biochem Biophys Res Commun 327:952-9. 2005
    ....
  18. ncbi request reprint Transcription patterns of PMA1 and PMA2 genes and activity of plasma membrane H+-ATPase in Saccharomyces cerevisiae during diauxic growth and stationary phase
    Alexandra R Fernandes
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, 1049 001 Lisboa, Portugal
    Yeast 20:207-19. 2003
    ....
  19. ncbi request reprint Insights into Pseudomonas putida KT2440 response to phenol-induced stress by quantitative proteomics
    Pedro M Santos
    Biological Sciences Research Group, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico IST, Lisboa, Portugal
    Proteomics 4:2640-52. 2004
    ..putida KT2440, will allow more rationale strategies for developing bacteria with greater solvent tolerance with impact in bioremediation and whole-cell biotransformations in media with organic solvents...
  20. ncbi request reprint AQR1 gene (ORF YNL065w) encodes a plasma membrane transporter of the major facilitator superfamily that confers resistance to short-chain monocarboxylic acids and quinidine in Saccharomyces cerevisiae
    Sandra Tenreiro
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Avenida Rovisco Pais, 1049 001 Lisbon, Portugal
    Biochem Biophys Res Commun 292:741-8. 2002
    ..The encoded putative transporter was localized in the plasma membrane by fluorescence microscopy observation of the overproduced Aqr1-GFP fusion protein distribution...
  21. pmc Studies on the involvement of the exopolysaccharide produced by cystic fibrosis-associated isolates of the Burkholderia cepacia complex in biofilm formation and in persistence of respiratory infections
    Mónica V Cunha
    Grupo de Ciências Biológicas, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    J Clin Microbiol 42:3052-8. 2004
    ....
  22. pmc Candida glabrata drug:H+ antiporter CgQdr2 confers imidazole drug resistance, being activated by transcription factor CgPdr1
    Catarina Costa
    Department of Bioengineering, Instituto Superior Tecnico, Technical University of Lisbon, Lisbon, Portugal
    Antimicrob Agents Chemother 57:3159-67. 2013
    ..glabrata...
  23. pmc The SPI1 gene, encoding a glycosylphosphatidylinositol-anchored cell wall protein, plays a prominent role in the development of yeast resistance to lipophilic weak-acid food preservatives
    T Simoes
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Appl Environ Microbiol 72:7168-75. 2006
    ..Decreased cell wall porosity, in turn, reduces access to the plasma membrane, reducing membrane damage, intracellular acidification, and viability loss...
  24. ncbi request reprint A proteome analysis of the yeast response to the herbicide 2,4-dichlorophenoxyacetic acid
    Miguel C Teixeira
    Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisbon, Portugal
    Proteomics 5:1889-901. 2005
    ....
  25. pmc Increased expression of the yeast multidrug resistance ABC transporter Pdr18 leads to increased ethanol tolerance and ethanol production in high gravity alcoholic fermentation
    Miguel C Teixeira
    IBB Institute for Biotechnology and Bioengineering, Centro de Engenharia Biologica e Quimica, and Department of Bioengineering, Instituto Superior Tecnico, Technical University of Lisbon, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Microb Cell Fact 11:98. 2012
    ..A set of 21 genes encoding multidrug transporters from the ATP-Binding Cassette (ABC) Superfamily and Major Facilitator Superfamily (MFS) in S. cerevisiae were scrutinized for a role in ethanol stress resistance...
  26. pmc Impact of assimilable nitrogen availability in glucose uptake kinetics in Saccharomyces cerevisiae during alcoholic fermentation
    Margarida Palma
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Technical University of Lisbon, 1049 001 Lisbon, Portugal
    Microb Cell Fact 11:99. 2012
    ..cerevisiae PYCC 4072, of a synthetic grape juice basal medium with either a limiting or non-limiting initial nitrogen concentration and following nitrogen supplementation of the nitrogen-depleted sluggish fermentation...
  27. pmc Identification of a DNA-binding site for the transcription factor Haa1, required for Saccharomyces cerevisiae response to acetic acid stress
    Nuno P Mira
    IBB, Instituto Biotecnologia e Bioengenharia, Center for Biological and Chemical Engineering, Instituto Superior Tecnico, Avenida Rovisco Pais, 1049 001 Lisbon, Portugal
    Nucleic Acids Res 39:6896-907. 2011
    ..Altogether, the results obtained indicate that the minimal functional binding site of Haa1 is 5'-(G/C)(A/C)GG(G/C)G-3'. The Haa1-dependent transcriptional regulatory network active in yeast response to acetic acid stress is proposed...
  28. doi request reprint The RIM101 pathway has a role in Saccharomyces cerevisiae adaptive response and resistance to propionic acid and other weak acids
    Nuno P Mira
    IBB, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Lisboa, Portugal
    FEMS Yeast Res 9:202-16. 2009
    ....
  29. doi request reprint Insights into the mechanisms of toxicity and tolerance to the agricultural fungicide mancozeb in yeast, as suggested by a chemogenomic approach
    Paulo J Dias
    IBB Institute for Biotechnology and Bioengineering, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisboa, Portugal
    OMICS 14:211-27. 2010
    ..This toxicogenomics analysis may, thus, increase the understanding of mancozeb toxicity and adaptation mechanisms in humans...
  30. ncbi request reprint Yeast adaptation to 2,4-dichlorophenoxyacetic acid involves increased membrane fatty acid saturation degree and decreased OLE1 transcription
    Cristina A Viegas
    Biological Sciences Research Group, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Biochem Biophys Res Commun 330:271-8. 2005
    ....
  31. doi request reprint Heterologous expression of a Tpo1 homolog from Arabidopsis thaliana confers resistance to the herbicide 2,4-D and other chemical stresses in yeast
    Tânia R Cabrito
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Appl Microbiol Biotechnol 84:927-36. 2009
    ..Heterologous expression of At5g13750 in yeast also leads to increased resistance to indole-3-acetic acid (IAA), Al(3+) and Tl(3+). At5g13750 is the first plant putative MFS transporter to be suggested as possibly involved in MDR...
  32. pmc Proteins encoded by Sphingomonas elodea ATCC 31461 rmlA and ugpG genes, involved in gellan gum biosynthesis, exhibit both dTDP- and UDP-glucose pyrophosphorylase activities
    Elisabete Silva
    Biological Sciences Research Group, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Appl Environ Microbiol 71:4703-12. 2005
    ....
  33. ncbi request reprint The herbicide 2,4-dichlorophenoxyacetic acid induces the generation of free-radicals and associated oxidative stress responses in yeast
    Miguel C Teixeira
    Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, 1049 001 Lisbon, Portugal
    Biochem Biophys Res Commun 324:1101-7. 2004
    ..The variation pattern of the level of hydroxyl-radical adducts correlated with the one determined for the activity of Sod1p, cytosolic catalase Ctt1p, and the dithiol glutaredoxins Grx1p and Grx2p...
  34. pmc Transcriptomic profiling of the Saccharomyces cerevisiae response to quinine reveals a glucose limitation response attributable to drug-induced inhibition of glucose uptake
    Sandra C dos Santos
    Institute for Biotechnology and Bioengineering IBB, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Lisbon, Portugal
    Antimicrob Agents Chemother 53:5213-23. 2009
    ..We propose that PfHT1 is an interesting candidate quinine target possibly involved in quinine import in P. falciparum, an uptake mechanism postulated in recent studies to occur through a still-unidentified importer(s)...
  35. pmc Saccharomyces cerevisiae multidrug resistance transporter Qdr2 is implicated in potassium uptake, providing a physiological advantage to quinidine-stressed cells
    Rita C Vargas
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Eukaryot Cell 6:134-42. 2007
    ..Second, the amino acid pool is higher in Deltaqdr2 cells than in wild-type cells, indicating that QDR2 expression is, directly or indirectly, involved in amino acid homeostasis...
  36. pmc Quantitative 1H-NMR-metabolomics reveals extensive metabolic reprogramming and the effect of the aquaglyceroporin FPS1 in ethanol-stressed yeast cells
    Artur B Lourenço
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Technical University of Lisbon, Lisboa, Portugal
    PLoS ONE 8:e55439. 2013
    ....
  37. pmc Genomic expression program involving the Haa1p-regulon in Saccharomyces cerevisiae response to acetic acid
    Nuno P Mira
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Technical University of Lisbon, Lisboa, Portugal
    OMICS 14:587-601. 2010
    ....
  38. pmc The yeast ABC transporter Pdr18 (ORF YNR070w) controls plasma membrane sterol composition, playing a role in multidrug resistance
    Tânia R Cabrito
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, 1049 001 Lisboa, Portugal
    Biochem J 440:195-202. 2011
    ..Taken together, the results of the present study suggest that Pdr18 plays a role in plasma membrane sterol incorporation, this physiological trait contributing to an MDR phenotype...
  39. pmc Burkholderia cenocepacia phenotypic clonal variation during a 3.5-year colonization in the lungs of a cystic fibrosis patient
    Carla P Coutinho
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Infect Immun 79:2950-60. 2011
    ..cenocepacia variants retrieved, along with the deterioration of pulmonary function and severe oxygen limitation...
  40. doi request reprint Response of Pseudomonas putida KT2440 to phenol at the level of membrane proteome
    Catarina Roma-Rodrigues
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Universidade Tecnica de Lisboa, Lisboa, Portugal
    J Proteomics 73:1461-78. 2010
    ..All these mechanistic insights may be extended to Pseudomonas adaptation to solvents, of possible impact in biodegradation, bioremediation and biocatalysis...
  41. ncbi request reprint The multidrug resistance transporters of the major facilitator superfamily, 6 years after disclosure of Saccharomyces cerevisiae genome sequence
    Isabel Sá-Correia
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    J Biotechnol 98:215-26. 2002
    ..Most of the present knowledge on these putative drug:H(+)-antiporters was driven by disclosure of S. cerevisiae genome sequence, in April 1996, being a paradigm of post-genomic research...
  42. doi request reprint Adaptive response to acetic acid in the highly resistant yeast species Zygosaccharomyces bailii revealed by quantitative proteomics
    Joana F Guerreiro
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Department of Bioengineering, Instituto Superior Tecnico, Technical University of Lisbon, Portugal
    Proteomics 12:2303-18. 2012
    ..When acetic acid is the sole carbon source, results suggest the activation of gluconeogenic and pentose phosphate pathways, based on the increased content of several proteins of these pathways after glucose exhaustion...
  43. pmc Genome-wide identification of Saccharomyces cerevisiae genes required for tolerance to acetic acid
    Nuno P Mira
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Technical University of Lisbon, Lisboa, Portugal
    Microb Cell Fact 9:79. 2010
    ..cerevisiae tolerance to acetic acid is essential for the rational selection of optimal fermentation conditions and the engineering of more robust industrial strains to be used in processes in which yeast is explored as cell factory...
  44. doi request reprint A genome-wide screen identifies yeast genes required for protection against or enhanced cytotoxicity of the antimalarial drug quinine
    Sandra C dos Santos
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001, Lisbon, Portugal
    Mol Genet Genomics 286:333-46. 2011
    ..falciparum context...
  45. ncbi request reprint Adaptive response to the antimalarial drug artesunate in yeast involves Pdr1p/Pdr3p-mediated transcriptional activation of the resistance determinants TPO1 and PDR5
    Marta Alenquer
    Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisbon, Portugal
    FEMS Yeast Res 6:1130-9. 2006
    ..Maximum TPO1 mRNA levels were rapidly reduced to basal values following adaptation of the yeast population to artesunate, while high PDR5 levels were maintained during drug-stressed exponential growth...
  46. ncbi request reprint Chromosomal organization and transcription analysis of genes in the vicinity of Pseudomonas aeruginosa glmM gene encoding phosphoglucosamine mutase
    Isabel M Tavares
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Biochem Biophys Res Commun 302:363-71. 2003
    ..Differently, transcription of genes in the glmM operon is more active in the early exponential phase, decreasing with the increase of cell density during exponential growth and reaching negligible values in stationary phase cells...
  47. pmc Functional and topological analysis of the Burkholderia cenocepacia priming glucosyltransferase BceB, involved in the biosynthesis of the cepacian exopolysaccharide
    Paula A Videira
    Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisbon, Portugal
    J Bacteriol 187:5013-8. 2005
    ..The results of protein topology analysis using PhoA and LacZ fusions supported in silico predictions that BceB has at least six transmembrane segments and two major cytoplasmic loops comprising the conserved regions described above...
  48. pmc The dual role of candida glabrata drug:H+ antiporter CgAqr1 (ORF CAGL0J09944g) in antifungal drug and acetic acid resistance
    Catarina Costa
    Department of Bioengineering, Instituto Superior Tecnico, Technical University of Lisbon Lisbon, Portugal Biological Sciences Research Group, Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Technical University of Lisbon Lisbon, Portugal
    Front Microbiol 4:170. 2013
    ..Results from this study show that CgAqr1 is an antifungal drug resistance determinant and raise the hypothesis that it may play a role in C. glabrata persistent colonization and multidrug resistance. ..
  49. pmc Long-term colonization of the cystic fibrosis lung by Burkholderia cepacia complex bacteria: epidemiology, clonal variation, and genome-wide expression alterations
    Carla P Coutinho
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Technical University of Lisbon, Lisbon, Portugal
    Front Cell Infect Microbiol 1:12. 2011
    ....
  50. doi request reprint Quantitative proteomics (2-D DIGE) reveals molecular strategies employed by Burkholderia cenocepacia to adapt to the airways of cystic fibrosis patients under antimicrobial therapy
    Andreia Madeira
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Department of Bioengineering, Instituto Superior Tecnico, Technical University of Lisbon, Lisbon, Portugal
    Proteomics 11:1313-28. 2011
    ..The quantitative comparison of the two proteomes suggests a genetic adaptation leading to increased antimicrobial resistance and bacterial persistence in the CF airways...
  51. doi request reprint Insights into yeast adaptive response to the agricultural fungicide mancozeb: a toxicoproteomics approach
    Pedro M Santos
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Lisboa, Portugal
    Proteomics 9:657-70. 2009
    ..Our results also suggest that this study provided powerful indications that may be useful to expand the knowledge obtained in yeast not only to the global response to mancozeb toxicity in phytopathogenic fungi but also to humans...
  52. pmc Adaptive response and tolerance to weak acids in Saccharomyces cerevisiae: a genome-wide view
    Nuno P Mira
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Technical University of Lisbon, Lisboa, Portugal
    OMICS 14:525-40. 2010
    ..The extrapolation of the knowledge gathered in yeast to other eukaryotes is also attempted...
  53. doi request reprint Adaptation to beta-myrcene catabolism in Pseudomonas sp. M1: an expression proteomics analysis
    Pedro M Santos
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, Lisbon, Portugal
    Proteomics 9:5101-11. 2009
    ....
  54. doi request reprint Genome-wide identification of genes required for yeast growth under imatinib stress: vacuolar H+-ATPase function is an important target of this anticancer drug
    Sandra C dos Santos
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Lisboa, Portugal
    OMICS 13:185-98. 2009
    ..Results suggest that imatinib might act as an effective inhibitor of V-ATPase function in yeast, identifying V-ATPase activity and vacuolar function as novel imatinib targets...
  55. pmc Molecular analysis of Burkholderia cepacia complex isolates from a Portuguese cystic fibrosis center: a 7-year study
    Mónica V Cunha
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Hospital de Santa Maria, Lisbon, Portugal
    J Clin Microbiol 41:4113-20. 2003
    ..Altogether, our results indicate that the use of these markers or the genomovar status in identifying patients at higher risk for infection is uncertain...
  56. ncbi request reprint The gellan gum biosynthetic genes gelC and gelE encode two separate polypeptides homologous to the activator and the kinase domains of tyrosine autokinases
    Leonilde M Moreira
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, PT 1049 001 Lisboa, Portugal
    J Mol Microbiol Biotechnol 8:43-57. 2004
    ..The tyrosine residue at position 198 appears to be essential for the synthesis of high-molecular-weight gellan, although other tyrosine residues may additionally contribute to GelE biological function...
  57. ncbi request reprint Characterization of the unique organization and co-regulation of a gene cluster required for phenol and benzene catabolism in Pseudomonas sp. M1
    Pedro M Santos
    IBB, Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001, Lisboa, Portugal
    J Biotechnol 131:371-8. 2007
    ..Both promoters are phenol and benzene inducible and evidence supporting the unique sigma(54)-dependent co-regulation of the phenol/benzene inducible genes phcA and phcKLMNOP, mediated by PhcR, was obtained...
  58. pmc Functional analysis of Burkholderia cepacia genes bceD and bceF, encoding a phosphotyrosine phosphatase and a tyrosine autokinase, respectively: role in exopolysaccharide biosynthesis and biofilm formation
    Ana S Ferreira
    Institute for Biotechnology and Bioengineering, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Appl Environ Microbiol 73:524-34. 2007
    ....
  59. ncbi request reprint The Burkholderia cepacia bceA gene encodes a protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities
    Silvia A Sousa
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Biochem Biophys Res Commun 353:200-6. 2007
    ..However, the viscosity of aqueous solutions prepared with the EPS produced by the bceA mutant was significantly reduced compared with wild-type biopolymer and the mutant forms biofilms with a size reduced by 6-fold...
  60. pmc Genome-wide identification of Saccharomyces cerevisiae genes required for maximal tolerance to ethanol
    Miguel C Teixeira
    Institute for Biotechnology and Bioengineering, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Appl Environ Microbiol 75:5761-72. 2009
    ..The increased expression of FPS1 confirmed the important role of this gene in alcoholic fermentation, leading to increased final ethanol concentration under conditions that lead to high ethanol production...
  61. pmc Proteomic Profiling of Burkholderia cenocepacia Clonal Isolates with Different Virulence Potential Retrieved from a Cystic Fibrosis Patient during Chronic Lung Infection
    Andreia Madeira
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Department of Bioengineering, Instituto Superior Tecnico, Universidade de Lisboa, Lisbon, Portugal
    PLoS ONE 8:e83065. 2013
    ....
  62. pmc Genomic expression analysis reveals strategies of Burkholderia cenocepacia to adapt to cystic fibrosis patients' airways and antimicrobial therapy
    Nuno P Mira
    IBB, Institute for Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, Instituto Superior Tecnico, Technical University of Lisbon, Lisbon, Portugal
    PLoS ONE 6:e28831. 2011
    ....
  63. doi request reprint Characterization of complex regulatory networks and identification of promoter regulatory elements in yeast: "in silico" and "wet-lab" approaches
    Nuno P Mira
    Institute for Biotechnology and Bioengineering, Technical University of Lisbon, Lisbon, Portugal
    Methods Mol Biol 809:27-48. 2012
    ..A set of easily accessible experimental approaches that can be used to confirm the predictions of the bioinformatic analysis is also detailed...
  64. doi request reprint Identification of regulatory modules in time series gene expression data using a linear time biclustering algorithm
    Sara C Madeira
    Universidade da Beira Interior, Covilha, KDBIO Group, INESC ID, Lisbon, Portugal
    IEEE/ACM Trans Comput Biol Bioinform 7:153-65. 2010
    ....
  65. doi request reprint A genome-wide perspective on the response and tolerance to food-relevant stresses in Saccharomyces cerevisiae
    Miguel C Teixeira
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Technical University of Lisbon, 1049 001 Lisboa, Portugal
    Curr Opin Biotechnol 22:150-6. 2011
    ..The extension of the data gathered in S. cerevisiae to food spoilage fungi is considered. The relevance of the different genome-wide approaches in this context is also discussed...
  66. ncbi request reprint Biochemical characterization and phylogenetic analysis of UDP-glucose dehydrogenase from the gellan gum producer Sphingomonas elodea ATCC 31461
    Ana Teresa Granja
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Appl Microbiol Biotechnol 76:1319-27. 2007
    ..DNA sequencing of chromosomal regions adjacent to ugdG gene and sequence similarity studies suggests that this gene maps together with others presumably involved in the biosynthesis of S. elodea cell wall polysaccharides...
  67. doi request reprint Occurrence, production, and applications of gellan: current state and perspectives
    Arsenio M Fialho
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Appl Microbiol Biotechnol 79:889-900. 2008
    ..In addition, a highlight of the importance of gellan in industrial and medicinal applications is given...
  68. pmc Yeast toxicogenomics: genome-wide responses to chemical stresses with impact in environmental health, pharmacology, and biotechnology
    Sandra C dos Santos
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Technical University of Lisbon Lisbon, Portugal
    Front Genet 3:63. 2012
    ....
  69. pmc Human mesenchymal stem cell expression program upon extended ex-vivo cultivation, as revealed by 2-DE-based quantitative proteomics
    Andreia Madeira
    Institute for Biotechnology and Bioengineering IBB, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Lisboa, Portugal
    PLoS ONE 7:e43523. 2012
    ....
  70. doi request reprint Teaching expression proteomics: From the wet-lab to the laptop
    Miguel C Teixeira
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Technical University of Lisbon, 1049 001 Lisboa, Portugal Department of Chemical and Biological Engineering, Instituto Superior Tecnico, Technical University of Lisbon, 1049 001 Lisboa, Portugal
    Biochem Mol Biol Educ 37:279-86. 2009
    ..The current role of quantitative proteomics in biological research, with emphasis on microbial stress response and on biomedical and biotechnological applications, is addressed, as a case-study, anchored on our group research activities...
  71. ncbi request reprint Mechanistic insights into the global response to phenol in the phenol-biodegrading strain Pseudomonas sp. M1 revealed by quantitative proteomics
    Pedro M Santos
    Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Lisboa, Portugal
    OMICS 11:233-51. 2007
    ..They were attributed to differences observed in the specific growth rate, in the expression of phenol catabolism, and in resistance to phenol of Pseudomonas sp. M1 grown under different conditions...
  72. ncbi request reprint [The clinical course of Burkholderia cepacia complex bacteria respiratory infection in cystic fibrosis patients]
    Susana Correia
    Departamento da Crianca e da Familia, Hospital de Santa Maria, Lisboa, Portugal
    Rev Port Pneumol 14:5-26. 2008
    ..Based on the available data, it is not currently possible to outline preventive measures through the molecular characterisation of Bcc isolates, reinforcing the notion that the recommended control measures must be followed...
  73. ncbi request reprint Environmental genomics: mechanistic insights into toxicity of and resistance to the herbicide 2,4-D
    Miguel Cacho Teixeira
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, 1049 001 Lisboa, Portugal
    Trends Biotechnol 25:363-70. 2007
    ..This review summarizes recent mechanistic insights into 2,4-D toxicity and the corresponding adaptive responses based on studies carried out using Saccharomyces cerevisiae and Arabidopsis thaliana as model organisms...