I Sá-Correia

Summary

Country: Portugal

Publications

  1. ncbi request reprint Comparative effects of Saccharomyces cerevisiae cultivation under copper stress on the activity and kinetic parameters of plasma-membrane-bound H(+)-ATPases PMA1 and PMA2
    A R Fernandes
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisboa, Portugal
    Arch Microbiol 171:273-8. 1999
  2. ncbi request reprint FLR1 gene (ORF YBR008c) is required for benomyl and methotrexate resistance in Saccharomyces cerevisiae and its benomyl-induced expression is dependent on pdr3 transcriptional regulator
    N Brôco
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Yeast 15:1595-608. 1999
  3. ncbi request reprint The activity of plasma membrane H(+)-ATPase is strongly stimulated during Saccharomyces cerevisiae adaptation to growth under high copper stress, accompanying intracellular acidification
    A R Fernandes
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, 1049 001 Lisboa, Portugal
    Yeast 18:511-21. 2001
  4. ncbi request reprint Expression of the AZR1 gene (ORF YGR224w), encoding a plasma membrane transporter of the major facilitator superfamily, is required for adaptation to acetic acid and resistance to azoles in Saccharomyces cerevisiae
    S Tenreiro
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Yeast 16:1469-81. 2000
  5. pmc Resistance and adaptation to quinidine in Saccharomyces cerevisiae: role of QDR1 (YIL120w), encoding a plasma membrane transporter of the major facilitator superfamily required for multidrug resistance
    P A Nunes
    Centro de Engenharia Biológia e Química, Instituto Superior Tecnico, 1049 001 Lisbon, Portugal
    Antimicrob Agents Chemother 45:1528-34. 2001
  6. ncbi request reprint Cloning and sequence analysis of the ces10 gene encoding a Sphingomonas paucimobilis esterase
    P A Videira
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Avenida Rovisco Pais, 1049 001 Lisboa, Portugal
    Appl Microbiol Biotechnol 61:517-22. 2003
  7. ncbi request reprint Characterization of the ugpG gene encoding a UDP-glucose pyrophosphorylase from the gellan gum producer Sphingomonas paucimobilis ATCC 31461
    A R Marques
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Mol Genet Genomics 268:816-24. 2003
  8. ncbi request reprint Saccharomyces cerevisiae adaptation to weak acids involves the transcription factor Haa1p and Haa1p-regulated genes
    A R Fernandes
    Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, 1049 001 Lisboa, Portugal
    Biochem Biophys Res Commun 337:95-103. 2005
  9. ncbi request reprint Activity of plasma membrane H+-ATPase and expression of PMA1 and PMA2 genes in Saccharomyces cerevisiae cells grown at optimal and low pH
    V Carmelo
    Laboratorio de Engenharia Bioquimica, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, P 1096 Lisboa Codex, Portugal
    Arch Microbiol 166:315-20. 1996
  10. ncbi request reprint Adaptative responses in yeast to the herbicide 2-methyl-4-chlorophenoxyacetic acid at the level of intracellular pH homeostasis
    M Guadalupe Cabral
    Biological Sciences Research Group, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Lisboa, Portugal
    J Appl Microbiol 96:603-12. 2004

Collaborators

  • A Sala
  • A R Fernandes
  • S Tenreiro
  • M C Teixeira
  • C A Viegas
  • A R Marques
  • M Guadalupe Cabral
  • A M Fialho
  • M G Cabral
  • P A Videira
  • P A Nunes
  • P J Dias
  • A T Freitas
  • P T Monteiro
  • A L Oliveira
  • N Brôco
  • N P Mira
  • R C Vargas
  • I Canelhas
  • P M Coutinho
  • P J Durão
  • P B Ferreira
  • P M Santos
  • V Carmelo
  • P C Rosa
  • P Bogaerts

Detail Information

Publications15

  1. ncbi request reprint Comparative effects of Saccharomyces cerevisiae cultivation under copper stress on the activity and kinetic parameters of plasma-membrane-bound H(+)-ATPases PMA1 and PMA2
    A R Fernandes
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisboa, Portugal
    Arch Microbiol 171:273-8. 1999
    ....
  2. ncbi request reprint FLR1 gene (ORF YBR008c) is required for benomyl and methotrexate resistance in Saccharomyces cerevisiae and its benomyl-induced expression is dependent on pdr3 transcriptional regulator
    N Brôco
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Yeast 15:1595-608. 1999
    ..Indeed, PDR3 deletion severely reduces benomyl-induced activation of FLR1 gene expression (by 85%), while the homologous Pdr1p transcription factor is apparently not involved in this activation...
  3. ncbi request reprint The activity of plasma membrane H(+)-ATPase is strongly stimulated during Saccharomyces cerevisiae adaptation to growth under high copper stress, accompanying intracellular acidification
    A R Fernandes
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, 1049 001 Lisboa, Portugal
    Yeast 18:511-21. 2001
    ....
  4. ncbi request reprint Expression of the AZR1 gene (ORF YGR224w), encoding a plasma membrane transporter of the major facilitator superfamily, is required for adaptation to acetic acid and resistance to azoles in Saccharomyces cerevisiae
    S Tenreiro
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Yeast 16:1469-81. 2000
    ....
  5. pmc Resistance and adaptation to quinidine in Saccharomyces cerevisiae: role of QDR1 (YIL120w), encoding a plasma membrane transporter of the major facilitator superfamily required for multidrug resistance
    P A Nunes
    Centro de Engenharia Biológia e Química, Instituto Superior Tecnico, 1049 001 Lisbon, Portugal
    Antimicrob Agents Chemother 45:1528-34. 2001
    ..However, we were not able to prove that Qdr1p is directly implicated in this export. Results clearly suggest that there are other unidentified quinidine resistance mechanisms that can be used in the absence of QDR1...
  6. ncbi request reprint Cloning and sequence analysis of the ces10 gene encoding a Sphingomonas paucimobilis esterase
    P A Videira
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Avenida Rovisco Pais, 1049 001 Lisboa, Portugal
    Appl Microbiol Biotechnol 61:517-22. 2003
    ..Ces10 exhibited a marked preference for short-chain fatty acids, yielding the highest activity with p-NP-propionate (optimal pH 7.4, optimal temperature 37 degrees C)...
  7. ncbi request reprint Characterization of the ugpG gene encoding a UDP-glucose pyrophosphorylase from the gellan gum producer Sphingomonas paucimobilis ATCC 31461
    A R Marques
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisbon, Portugal
    Mol Genet Genomics 268:816-24. 2003
    ..Kinetic analysis of UgpG in the reverse reaction (pyrophosphorolysis) showed a typical Michaelis-Menten substrate saturation pattern. The apparent K(m) and V(max) values estimated for UDP-glucose were 7.5 microM and 1275 micromol/min/g...
  8. ncbi request reprint Saccharomyces cerevisiae adaptation to weak acids involves the transcription factor Haa1p and Haa1p-regulated genes
    A R Fernandes
    Biological Sciences Research Group, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, 1049 001 Lisboa, Portugal
    Biochem Biophys Res Commun 337:95-103. 2005
    ..The acetic acid-induced prolongation of the lag phase of unadapted cell populations lacking HAA1 or TPO3, compared with wild-type population, was correlated with the level of the acid accumulated into the stressed cells...
  9. ncbi request reprint Activity of plasma membrane H+-ATPase and expression of PMA1 and PMA2 genes in Saccharomyces cerevisiae cells grown at optimal and low pH
    V Carmelo
    Laboratorio de Engenharia Bioquimica, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, P 1096 Lisboa Codex, Portugal
    Arch Microbiol 166:315-20. 1996
    ..As observed in response to other kinds of stress, the PMA2 promoter at the optimal pH was up to eightfold more efficient in cells grown at pH 2.5, although it remained several hundred times less efficient than that of the PMA1 gene...
  10. ncbi request reprint Adaptative responses in yeast to the herbicide 2-methyl-4-chlorophenoxyacetic acid at the level of intracellular pH homeostasis
    M Guadalupe Cabral
    Biological Sciences Research Group, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Lisboa, Portugal
    J Appl Microbiol 96:603-12. 2004
    ..The objective of this work was to examine adaptative responses occurring in Saccharomyces cerevisiae following exposure to the herbicide 2-methyl-4-chlorophenoxyacetic acid (MCPA)...
  11. ncbi request reprint Toxicity of chlorinated phenoxyacetic acid herbicides in the experimental eukaryotic model Saccharomyces cerevisiae: role of pH and of growth phase and size of the yeast cell population
    M G Cabral
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001, Lisboa, Portugal
    Chemosphere 51:47-54. 2003
    ....
  12. ncbi request reprint Transcriptional activation of FLR1 gene during Saccharomyces cerevisiae adaptation to growth with benomyl: role of Yap1p and Pdr3p
    S Tenreiro
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Avenida Rovisco Pais, 1049 001 Lisbon, Portugal
    Biochem Biophys Res Commun 280:216-22. 2001
    ..Although benomyl resistance mediated by Yap1p is reduced in a FLR1 deletion mutant, results also indicate that Yap1p may have other target genes that confer benomyl resistance in yeast...
  13. doi request reprint Refining current knowledge on the yeast FLR1 regulatory network by combined experimental and computational approaches
    M C Teixeira
    IBB Institute for Biotechnology and Bioengineering, Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Technical University of Lisbon, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Mol Biosyst 6:2471-81. 2010
    ....
  14. ncbi request reprint Gellan gum biosynthesis in Sphingomonas paucimobilis ATCC 31461: genes, enzymes and exopolysaccharide production engineering
    I Sá-Correia
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Lisbon 1049 001, Portugal
    J Ind Microbiol Biotechnol 29:170-6. 2002
    ....
  15. ncbi request reprint Activation and significance of vacuolar H+-ATPase in Saccharomyces cerevisiae adaptation and resistance to the herbicide 2,4-dichlorophenoxyacetic acid
    A R Fernandes
    Biological Sciences Research Group, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, 1049 001, Lisbon, Portugal
    Biochem Biophys Res Commun 312:1317-24. 2003
    ..Results suggest that a functional V-ATPase is required to counteract, more rapidly and efficiently, the dissipation of the physiological H(+)-gradient across vacuolar membrane registered during 2,4-D adaptation...