Jorge Azevedo

Summary

Affiliation: ICBAS/IBMC-University of Porto
Location: Porto, Portugal
Summary:

Publications

  1. ncbi request reprint The import competence of a peroxisomal membrane protein is determined by Pex19p before the docking step
    Manuel P Pinto
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Biol Chem 281:34492-502. 2006
  2. ncbi request reprint Pex14p, more than just a docking protein
    Jorge E Azevedo
    Instituto de Biologia Molecular e Celular IBMC and Instituto de Ciências Biomédicas de Abel Salazar ICBAS, Univ do Porto, Portugal
    Biochim Biophys Acta 1763:1574-84. 2006
  3. doi request reprint High-yield expression in Escherichia coli and purification of mouse ubiquitin-activating enzyme E1
    Andreia F Carvalho
    Instituto de Biologia Molecular e Celular IBMC, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Mol Biotechnol 51:254-61. 2012
  4. ncbi request reprint The N-terminal half of the peroxisomal cycling receptor Pex5p is a natively unfolded domain
    Andreia F Carvalho
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Mol Biol 356:864-75. 2006
  5. ncbi request reprint AIP56, a novel plasmid-encoded virulence factor of Photobacterium damselae subsp. piscicida with apoptogenic activity against sea bass macrophages and neutrophils
    Ana do Vale
    Institute for Molecular and Cell Biology, Rua do Campo Alegre, 823 4150 180 Porto, Portugal
    Mol Microbiol 58:1025-38. 2005
  6. ncbi request reprint Pex5p, the peroxisomal cycling receptor, is a monomeric non-globular protein
    João Costa-Rodrigues
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Biol Chem 280:24404-11. 2005
  7. ncbi request reprint HFE cross-talks with the MHC class I antigen presentation pathway
    Sérgio F de Almeida
    Iron Genes and Immune System, IBMC, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Blood 106:971-7. 2005
  8. ncbi request reprint Probing substrate-induced conformational alterations in adrenoleukodystrophy protein by proteolysis
    Carla P Guimaraes
    UNILIPE, Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Hum Genet 50:99-105. 2005
  9. ncbi request reprint Protein translocation across the peroxisomal membrane
    Jorge E Azevedo
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Cell Biochem Biophys 41:451-68. 2004
  10. ncbi request reprint The N terminus of the peroxisomal cycling receptor, Pex5p, is required for redirecting the peroxisome-associated peroxin back to the cytosol
    João Costa-Rodrigues
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Instituto de Ciencias Biomedicas de Abel Salazar, Largo do Professor Abel Salazar, 2, 4099 003 Porto, Portugal
    J Biol Chem 279:46573-9. 2004

Collaborators

Detail Information

Publications47

  1. ncbi request reprint The import competence of a peroxisomal membrane protein is determined by Pex19p before the docking step
    Manuel P Pinto
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Biol Chem 281:34492-502. 2006
    ..These results suggest that soluble Pex19p participates in the targeting of newly synthesized peroxisomal membrane proteins to the organelle membrane and support the existence of a cargo-induced peroxisomal targeting mechanism for Pex19p...
  2. ncbi request reprint Pex14p, more than just a docking protein
    Jorge E Azevedo
    Instituto de Biologia Molecular e Celular IBMC and Instituto de Ciências Biomédicas de Abel Salazar ICBAS, Univ do Porto, Portugal
    Biochim Biophys Acta 1763:1574-84. 2006
    ..The available evidence suggests that the key to understand how folded proteins are capable of passing an apparently impermeable membrane may largely reside in this pair of peroxins...
  3. doi request reprint High-yield expression in Escherichia coli and purification of mouse ubiquitin-activating enzyme E1
    Andreia F Carvalho
    Instituto de Biologia Molecular e Celular IBMC, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Mol Biotechnol 51:254-61. 2012
    ..In this report, we show that 5-10 mg of histidine-tagged mouse E1 can be easily obtained from a 1 l E. coli culture. A low temperature during the protein induction step was found to be critical to obtain an active enzyme...
  4. ncbi request reprint The N-terminal half of the peroxisomal cycling receptor Pex5p is a natively unfolded domain
    Andreia F Carvalho
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Mol Biol 356:864-75. 2006
    ..Our results indicate that the N-terminal half of Pex5p is best described as a natively unfolded pre-molten globule-like domain. The implications of these findings on the mechanism of protein import into the peroxisome are discussed...
  5. ncbi request reprint AIP56, a novel plasmid-encoded virulence factor of Photobacterium damselae subsp. piscicida with apoptogenic activity against sea bass macrophages and neutrophils
    Ana do Vale
    Institute for Molecular and Cell Biology, Rua do Campo Alegre, 823 4150 180 Porto, Portugal
    Mol Microbiol 58:1025-38. 2005
    ....
  6. ncbi request reprint Pex5p, the peroxisomal cycling receptor, is a monomeric non-globular protein
    João Costa-Rodrigues
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Biol Chem 280:24404-11. 2005
    ..Our results show that Pex5p is a monomeric protein with an abnormal shape. The implications of these findings on current models of protein translocation across the peroxisomal membrane are discussed...
  7. ncbi request reprint HFE cross-talks with the MHC class I antigen presentation pathway
    Sérgio F de Almeida
    Iron Genes and Immune System, IBMC, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Blood 106:971-7. 2005
    ..These findings constitute the first description of peptide presentation pathway abnormalities linked to HFE and provide additional evidence for the occurrence of immunologic defects in patients with HH...
  8. ncbi request reprint Probing substrate-induced conformational alterations in adrenoleukodystrophy protein by proteolysis
    Carla P Guimaraes
    UNILIPE, Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Hum Genet 50:99-105. 2005
    ..Our results suggest that ALDP is directly involved in the transport of long- and very-long-chain acyl-CoAs across the peroxisomal membrane...
  9. ncbi request reprint Protein translocation across the peroxisomal membrane
    Jorge E Azevedo
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Cell Biochem Biophys 41:451-68. 2004
    ..These results point to a model in which proteins en route to the peroxisomal matrix are translocated across the organelle membrane by their own receptor in a process that occurs through a large membrane protein assembly...
  10. ncbi request reprint The N terminus of the peroxisomal cycling receptor, Pex5p, is required for redirecting the peroxisome-associated peroxin back to the cytosol
    João Costa-Rodrigues
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Instituto de Ciencias Biomedicas de Abel Salazar, Largo do Professor Abel Salazar, 2, 4099 003 Porto, Portugal
    J Biol Chem 279:46573-9. 2004
    ..Our data indicate that the export step of Pex5p from the peroxisomal compartment (in contrast with its insertion into the organelle membrane) is highly dependent on the temperature...
  11. ncbi request reprint Stimulation of an unfolded protein response impairs MHC class I expression
    Sérgio F de Almeida
    Iron Genes and Immune System Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    J Immunol 178:3612-9. 2007
    ..The present results provide evidence to the finding that stimulation of an UPR affects MHC-I expression...
  12. ncbi request reprint Functional characterization of two missense mutations in Pex5p - C11S and N526K
    Andreia F Carvalho
    Instituto de Biologia Molecular e Celular IBMC, Porto, Portugal
    Biochim Biophys Acta 1773:1141-8. 2007
    ..The implications of these findings on the mechanism of protein translocation across the peroxisomal membrane are discussed...
  13. ncbi request reprint Chemical chaperones reduce endoplasmic reticulum stress and prevent mutant HFE aggregate formation
    Sérgio F de Almeida
    Iron Genes and the Immune System Laboratory, Instituto de Biologia, Molecular e Celular, Universidade do Porto and Instituto de Ciências Biomédicas Abel Salazar, 4150 180 Porto, Portugal
    J Biol Chem 282:27905-12. 2007
    ..Together, these data shed light on the molecular mechanisms involved in HFE C282Y-related HH and open new perspectives on the use of orally active chemical chaperones as a therapeutic approach for HH...
  14. pmc PEX5 protein binds monomeric catalase blocking its tetramerization and releases it upon binding the N-terminal domain of PEX14
    Marta O Freitas
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Biol Chem 286:40509-19. 2011
    ....
  15. ncbi request reprint The cytosolic domain of PEX3, a protein involved in the biogenesis of peroxisomes, binds membrane lipids
    Manuel P Pinto
    Instituto de Biologia Molecular e Celular IBMC, Universidade do Porto, 4150 180 Porto, Portugal
    Biochim Biophys Acta 1793:1669-75. 2009
    ..The implications of these findings are discussed...
  16. pmc Mapping the cargo protein membrane translocation step into the PEX5 cycling pathway
    Inês S Alencastre
    Instituto de Biologia Molecular e Celular IBMC, Universidade do Porto, 4150 180 Porto, Portugal
    J Biol Chem 284:27243-51. 2009
    ..The data obtained with it suggest that translocation of a cargo protein across the peroxisomal membrane, including its release into the organelle matrix, occurs prior to PEX5 ubiquitination...
  17. pmc Properties of the ubiquitin-pex5p thiol ester conjugate
    Cláudia P Grou
    Instituto de Biologia Molecular e Celular IBMC, Universidade do Porto, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Biol Chem 284:10504-13. 2009
    ..Additionally, we show that MG132, a proteasome inhibitor, blocks the import of a peroxisomal reporter protein in vivo...
  18. doi request reprint The peroxisomal protein import machinery--a case report of transient ubiquitination with a new flavor
    C P Grou
    Instituto de Biologia Molecular e Celular IBMC, Universidade do Porto, Rua do Campo Alegre, 823, 4150 180, Porto, Portugal
    Cell Mol Life Sci 66:254-62. 2009
    ..Here, we discuss these data trying to distill the principles by which this complex machinery operates...
  19. doi request reprint A nonsense mutation in the LIMP-2 gene associated with progressive myoclonic epilepsy and nephrotic syndrome
    Andrea Balreira
    Unidade de Biologia do Lisossoma e do Peroxissoma, Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal
    Hum Mol Genet 17:2238-43. 2008
    ..This is the first report of a mutation in the SCARB2 gene associated with a human disease, which, contrary to earlier proposals, shares no features with Charcot-Marie-Tooth disease both at the clinical and neurophysiological levels...
  20. doi request reprint Members of the E2D (UbcH5) family mediate the ubiquitination of the conserved cysteine of Pex5p, the peroxisomal import receptor
    Cláudia P Grou
    Instituto de Biologia Molecular e Celular IBMC, Universidade do Porto, Rua do Campo Alegre, Porto, Portugal
    J Biol Chem 283:14190-7. 2008
    ..Here, we provide evidence suggesting that E2D1/2/3 (UbcH5a/b/c) are the mammalian functional counterparts of yeast/plant Pex4p. The mechanistic implications of these findings are discussed...
  21. ncbi request reprint Proteomics characterization of mouse kidney peroxisomes by tandem mass spectrometry and protein correlation profiling
    Sebastian Wiese
    Medizinisches Proteom Center, Ruhr Universitaet Bochum, Universitaetsstrasse 150, 44780 Bochum, Germany
    Mol Cell Proteomics 6:2045-57. 2007
    ..As a result of this joint effort, we believe to have compiled the so far most comprehensive protein catalogue of mammalian peroxisomes...
  22. ncbi request reprint Ubiquitination of mammalian Pex5p, the peroxisomal import receptor
    Andreia F Carvalho
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    J Biol Chem 282:31267-72. 2007
    ..Together with mechanistic data recently described for yeast Pex5p, these findings provide strong evidence for the existence of Pex4p- and Pex22p-like proteins in mammals...
  23. ncbi request reprint Mouse liver PMP70 and ALDP: homomeric interactions prevail in vivo
    Carla P Guimaraes
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, Porto, Portugal
    Biochim Biophys Acta 1689:235-43. 2004
    ..Our data indicate that the majority (if not all) of mouse liver PMP70 and ALDP are homomeric proteins...
  24. ncbi request reprint Characterization of the peroxisomal cycling receptor Pex5p import pathway
    Alexandra M Gouveia
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    Adv Exp Med Biol 544:219-20. 2003
  25. ncbi request reprint The energetics of Pex5p-mediated peroxisomal protein import
    Marcia E Oliveira
    Instituto de Biologia Molecular e Celular, 4150 180 Porto, Portugal
    J Biol Chem 278:39483-8. 2003
    ..This observation raises the possibility that at the peroxisomal membrane ATP is needed predominantly (if not exclusively) downstream of the protein translocation step to reset the Pex5p-mediated transport system...
  26. pmc In organello assembly of respiratory-chain complex I: primary structure of the 14.8 kDa subunit of Neurospora crassa complex I
    J E Azevedo
    Institut fur Physiologische Chemie, Universitat Munchen, Federal Republic of Germany
    Biochem J 299:297-302. 1994
    ..Furthermore, a fraction of the in-vitro-imported subunit was found to assemble in complex I. This is the first time that assembly in complex I of an in-vitro-synthesized subunit is demonstrated...
  27. ncbi request reprint Complementary DNA sequences of the 24 kDa and 21 kDa subunits of complex I from Neurospora
    J E Azevedo
    Instituto de Ciencias Biomedicas de Abel Salazar, Universidade do Porto, Portugal
    Biochim Biophys Acta 1188:159-61. 1994
    ..The two polypeptides are synthesized as precursor proteins which are processed to mature forms with predicted molecular masses of 24331 and 20982 Da...
  28. ncbi request reprint Characterization of the 9.5-kDa ubiquinone-binding protein of NADH:ubiquinone oxidoreductase (complex I) from Neurospora crassa
    H Heinrich
    Institut fur Physiologische Chemie, Universitat Munchen, Germany
    Biochemistry 31:11420-4. 1992
    ..This nuclearly-encoded subunit lacks a typical cleavable presequence and is imported into isolated mitochondria by a membrane potential-dependent process...
  29. pmc Cloning, in vitro mitochondrial import and membrane assembly of the 17.8 kDa subunit of complex I from Neurospora crassa
    J E Azevedo
    Institut fur Physiologische Chemie, Universitat Munchen, Germany
    Biochem J 293:501-6. 1993
    ..However, a significant fraction of the imported polypeptide acquires the same membrane topology as the endogenous subunit, indicating that correct assembly in the mitochondrial inner membrane did occur...
  30. ncbi request reprint Identification of a 24 kDa intrinsic membrane protein from mammalian peroxisomes
    C Reguenga
    Unidade de Neurobiologia Genética do Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal
    Biochim Biophys Acta 1445:337-41. 1999
    ..Hydropathy analysis reveals the existence of two putative membrane-spanning domains in conserved regions of the three homologous proteins...
  31. ncbi request reprint Alkaline density gradient floatation of membranes: polypeptide composition of the mammalian peroxisomal membrane
    A M Gouveia
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal
    Anal Biochem 274:270-7. 1999
    ..The identity of several of these polypeptides was determined by N-terminal sequencing and Western blotting analysis...
  32. ncbi request reprint Selective detection of UCP 3 expression in skeletal muscle: effect of thyroid status and temperature acclimation
    O Cunningham
    Department of Biochemistry, Trinity College Dublin, Biotechnology Building, Room 0 16, Dublin 2, Ireland
    Biochim Biophys Acta 1604:170-9. 2003
    ..In addition, we show that UCP 3 expression is only coincident with the mitochondrial fraction of skeletal muscle homogenates and not peroxisomal, nuclear or cytosolic and microsomal fractions...
  33. ncbi request reprint Oligomerization capacity of two arylsulfatase A mutants: C300F and P425T
    Ana Marcao
    Unidade da Biologia do Lisossoma e do Peroxissoma do Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal
    Biochem Biophys Res Commun 306:293-7. 2003
    ..Our data support the notion that the reduced lysosomal half-life of some mutated forms of ARSA is related to deficient octamerization...
  34. ncbi request reprint Insertion of Pex5p into the peroxisomal membrane is cargo protein-dependent
    Alexandra M Gouveia
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Biol Chem 278:4389-92. 2003
    ..These results suggest that the cytosol/peroxisomal cycle in which Pex5p is involved is directly or indirectly regulated by Pex5p itself and not by the peroxisomal docking/translocation machinery...
  35. ncbi request reprint Mammalian Pex14p: membrane topology and characterisation of the Pex14p-Pex14p interaction
    Márcia E M Oliveira
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal
    Biochim Biophys Acta 1567:13-22. 2002
    ..Heterologous expressed Pex14p was found to be a homopolymer of variable stoichiometry. Finally, in vitro binding assays indicate that homopolymerisation of Pex14p involves a domain comprising amino acid residues 147-278 of this peroxin...
  36. ncbi request reprint Characterization of the peroxisomal cycling receptor, Pex5p, using a cell-free in vitro import system
    Alexandra M Gouveia
    Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150 180 Porto, Portugal
    J Biol Chem 278:226-32. 2003
    ....
  37. ncbi request reprint Molecular characterization of 21 X-ALD Portuguese families: identification of eight novel mutations in the ABCD1 gene
    Carla P Guimaraes
    Department of Genetic Neurobiology, IBMC, Porto University, Portugal
    Mol Genet Metab 76:62-7. 2002
    ..ALDP was found at normal levels in only three patients, absent in five (corresponding to a double missense, two nonsense, and two frameshift mutations), and decreased in all the others...
  38. ncbi request reprint Characterisation of two mutations in the ABCD1 gene leading to low levels of normal ALDP
    C P Guimarães
    Genetic Neurobiology Department, IBMC Oporto University, Rua do Campo Alegre, 823, 4150 180 Oporto, Portugal
    Hum Genet 109:616-22. 2001
    ..It is proposed that the quantification of ALDP levels in these patients could provide important insights concerning the correlation between clinical phenotype and amount of normal ALDP...
  39. ncbi request reprint Characterization of the mammalian peroxisomal import machinery: Pex2p, Pex5p, Pex12p, and Pex14p are subunits of the same protein assembly
    C Reguenga
    Instituto de Biologia Molecular e Celular and Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 4150 180 Porto, Portugal
    J Biol Chem 276:29935-42. 2001
    ..Taken together, our data indicate that Pex2p, Pex5p, Pex12p, and Pex14p, on one side, and Pex13p, on the other, are subunits of two stable protein complexes that probably interact with each other in the peroxisomal membrane...