Cecilia M P Rodrigues

Summary

Country: Portugal

Publications

  1. pmc miR-34a regulates mouse neural stem cell differentiation
    Marcia M Aranha
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    PLoS ONE 6:e21396. 2011
  2. pmc TAp63γ demethylation regulates protein stability and cellular distribution during neural stem cell differentiation
    Maria B Fonseca
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    PLoS ONE 7:e52417. 2012
  3. pmc miR-143 overexpression impairs growth of human colon carcinoma xenografts in mice with induction of apoptosis and inhibition of proliferation
    Pedro M Borralho
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    PLoS ONE 6:e23787. 2011
  4. pmc Human colon cancer profiles show differential microRNA expression depending on mismatch repair status and are characteristic of undifferentiated proliferative states
    Aaron L Sarver
    Biostatistics and Informatics, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA
    BMC Cancer 9:401. 2009
  5. pmc Bilirubin and amyloid-beta peptide induce cytochrome c release through mitochondrial membrane permeabilization
    C M Rodrigues
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, Portugal
    Mol Med 6:936-46. 2000
  6. ncbi request reprint Tauroursodeoxycholic acid prevents Bax-induced membrane perturbation and cytochrome C release in isolated mitochondria
    Cecilia M P Rodrigues
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Lisbon 1600 083, Portugal
    Biochemistry 42:3070-80. 2003
  7. ncbi request reprint Perturbation of membrane dynamics in nerve cells as an early event during bilirubin-induced apoptosis
    Cecilia M P Rodrigues
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, 1600 083 Lisbon, Portugal
    J Lipid Res 43:885-94. 2002
  8. ncbi request reprint Bilirubin induces apoptosis via the mitochondrial pathway in developing rat brain neurons
    Cecilia M P Rodrigues
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, Lisbon, Portugal
    Hepatology 35:1186-95. 2002
  9. ncbi request reprint Bilirubin directly disrupts membrane lipid polarity and fluidity, protein order, and redox status in rat mitochondria
    Cecilia M P Rodrigues
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, Av Forcas Armadas, 1600 083, Lisbon, Portugal
    J Hepatol 36:335-41. 2002
  10. ncbi request reprint The therapeutic effects of ursodeoxycholic acid as an anti-apoptotic agent
    C M Rodrigues
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, Av Forcas Armadas, 1600 083 Lisbon, Portugal
    Expert Opin Investig Drugs 10:1243-53. 2001

Collaborators

  • Susana Sola
  • Betsy T Kren
  • Rui Moreira
  • Helena Cortez-Pinto
  • Maria José U Ferreira
  • Maria M M Santos
  • Maria A Brito
  • Yan Zeng
  • Raquel B Dias
  • Fabio Fernandes
  • Saikat Dewanjee
  • Ana Coutinho
  • Rudi D'Hooge
  • Paul A Iaizzo
  • Aaron L Sarver
  • Kristen Jepsen
  • Claudio M Gomes
  • D Brites
  • Rui E Castro
  • Clifford J Steer
  • Joana D Amaral
  • Rita M Ramalho
  • Pedro M Borralho
  • Ricardo J S Viana
  • Tayyab A Mansoor
  • Marcia M Aranha
  • Ana F Nunes
  • Maria B Fonseca
  • Daniela M Santos
  • Duarte M S Ferreira
  • Joana M Xavier
  • Diane M Pereira
  • Walter C Low
  • Pedro M Rodrigues
  • Carlos J A Ribeiro
  • Adrian C Lo
  • André E S Simões
  • Silva Mulhovo
  • Adilia Costa
  • Isabel B Moreira da Silva
  • Maria E Camilo
  • Marta B Afonso
  • Maria João Nunes
  • João Mello-Vieira
  • Sofia E Gomes
  • Xuan Luo
  • Ana L Morgado
  • Mariana Verdelho Machado
  • Paulo S Ribeiro
  • Miguel C Moura
  • Rui F M Silva
  • André L Simão
  • Maria João Gama
  • Pedro A Dionísio
  • Ana M Sebastião
  • Elsa Rodrigues
  • Manuel Prieto
  • Stephen N Thibodeau
  • Susana D Lucas
  • Tânia Sousa
  • Miguel Moutinho
  • Aleksander Fedorov
  • Mariana V Machado
  • Zsuzsanna Callaerts-Vegh
  • R F Silva
  • Fátima Carepa
  • Teresinha Evangelista
  • Joao Coutinho
  • Ana Rita Silvestre
  • Cátia Ramalhete
  • Maria H Vasconcelos
  • Tânia Carvalho
  • Raquel T Lima
  • Paulo M C Glória
  • Xiaoxiao Zhang
  • Ana R Correia
  • Miguel Carneiro de Moura
  • Cristina Albuquerque
  • Carlos Nobre Leitão
  • João D Arrabaça
  • Ana Teresa Mendes
  • Ana R Matos
  • Vera Vaz Pinto
  • Xiaoming Ma
  • Amelia Baptista

Detail Information

Publications72

  1. pmc miR-34a regulates mouse neural stem cell differentiation
    Marcia M Aranha
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    PLoS ONE 6:e21396. 2011
    ..However, the function of miR-34a in the control of the differentiation program of specific neural cell types remains largely unknown. Here, we investigated the role of miR-34a in regulating mouse neural stem (NS) cell differentiation...
  2. pmc TAp63γ demethylation regulates protein stability and cellular distribution during neural stem cell differentiation
    Maria B Fonseca
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    PLoS ONE 7:e52417. 2012
    ..These findings clarify the role of p63 in adult neural progenitor cells and reveal TAp63γ as a direct target for JMJD3-mediated neuronal commitment...
  3. pmc miR-143 overexpression impairs growth of human colon carcinoma xenografts in mice with induction of apoptosis and inhibition of proliferation
    Pedro M Borralho
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    PLoS ONE 6:e23787. 2011
    ..In the present study, we evaluated the role of miR-143 overexpression on the growth of human colon carcinoma cells xenografted in nude mice (immunodeficient mouse strain: N: NIH(s) II-nu/nu)...
  4. pmc Human colon cancer profiles show differential microRNA expression depending on mismatch repair status and are characteristic of undifferentiated proliferative states
    Aaron L Sarver
    Biostatistics and Informatics, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA
    BMC Cancer 9:401. 2009
    ..Differential miRNA expression in cancer versus normal tissue is a common event and may be pivotal for tumor onset and progression...
  5. pmc Bilirubin and amyloid-beta peptide induce cytochrome c release through mitochondrial membrane permeabilization
    C M Rodrigues
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, Portugal
    Mol Med 6:936-46. 2000
    ..In addition, we evaluate whether the anti-apoptotic drugs UDC and TUDC prevent any changes from occurring...
  6. ncbi request reprint Tauroursodeoxycholic acid prevents Bax-induced membrane perturbation and cytochrome C release in isolated mitochondria
    Cecilia M P Rodrigues
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Lisbon 1600 083, Portugal
    Biochemistry 42:3070-80. 2003
    ..TUDCA is a potent inhibitor of Bax association with mitochondria. Thus, TUDCA modulates apoptosis by suppressing mitochondrial membrane perturbation through pathways that are also independent of the mitochondrial permeability transition...
  7. ncbi request reprint Perturbation of membrane dynamics in nerve cells as an early event during bilirubin-induced apoptosis
    Cecilia M P Rodrigues
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, 1600 083 Lisbon, Portugal
    J Lipid Res 43:885-94. 2002
    ..Bilirubin directly interacts with membranes influencing lipid polarity and fluidity, protein order, and redox status. These data suggest that nerve cell membranes are primary targets of bilirubin toxicity...
  8. ncbi request reprint Bilirubin induces apoptosis via the mitochondrial pathway in developing rat brain neurons
    Cecilia M P Rodrigues
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, Lisbon, Portugal
    Hepatology 35:1186-95. 2002
    ..These results provide a novel insight into the mechanism of bilirubin-induced toxicity...
  9. ncbi request reprint Bilirubin directly disrupts membrane lipid polarity and fluidity, protein order, and redox status in rat mitochondria
    Cecilia M P Rodrigues
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, Av Forcas Armadas, 1600 083, Lisbon, Portugal
    J Hepatol 36:335-41. 2002
    ..Unconjugated bilirubin (UCB) impairs crucial aspects of cell function and induces apoptosis in primary cultured neurones. While mechanisms of cytotoxicity begin to unfold, mitochondria appear as potential primary targets...
  10. ncbi request reprint The therapeutic effects of ursodeoxycholic acid as an anti-apoptotic agent
    C M Rodrigues
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, Av Forcas Armadas, 1600 083 Lisbon, Portugal
    Expert Opin Investig Drugs 10:1243-53. 2001
    ....
  11. ncbi request reprint Aging confers different sensitivity to the neurotoxic properties of unconjugated bilirubin
    Cecilia M P Rodrigues
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, 1600 083 Lisbon, Portugal
    Pediatr Res 51:112-8. 2002
    ..Thus, although playing a role, direct mitochondrial injury may not be the sole mechanism of UCB cytotoxicity...
  12. ncbi request reprint Modulation of nuclear steroid receptors by ursodeoxycholic acid inhibits TGF-beta1-induced E2F-1/p53-mediated apoptosis of rat hepatocytes
    Susana Sola
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Lisbon 1600 083, Portugal
    Biochemistry 43:8429-38. 2004
    ..Further, the E2F-1/Mdm-2/p53 apoptotic pathway appears to be a prime target for UDCA-induced steroid receptor activation...
  13. ncbi request reprint Tauroursodeoxycholic acid modulates p53-mediated apoptosis in Alzheimer's disease mutant neuroblastoma cells
    Rita M Ramalho
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Portugal
    J Neurochem 98:1610-8. 2006
    ..In conclusion, FAD mutations are associated with the activation of classical apoptotic pathways. TUDCA reduces p53-induced apoptosis and modulates expression of Bcl-2 family...
  14. ncbi request reprint Inhibition of the E2F-1/p53/Bax pathway by tauroursodeoxycholic acid in amyloid beta-peptide-induced apoptosis of PC12 cells
    Rita M Ramalho
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    J Neurochem 90:567-75. 2004
    ..In conclusion, the results demonstrate that Abeta-induced apoptosis of PC12 cells proceeds through an E2F-1/p53/Bax pathway, which, in turn, can be specifically inhibited by TUDCA, thus underscoring its potential therapeutic use...
  15. pmc Efficient recovery of proteins from multiple source samples after TRIzol(®) or TRIzol(®)LS RNA extraction and long-term storage
    André E S Simões
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    BMC Genomics 14:181. 2013
    ..These reagents are widely used for RNA and/or DNA isolation, while reports on their use for protein extraction are limited, attributable to technical difficulties in protein solubilisation...
  16. ncbi request reprint Membrane structural changes support the involvement of mitochondria in the bile salt-induced apoptosis of rat hepatocytes
    Susana Sola
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, Lisbon, Av das Forcas Armadas, Portugal
    Clin Sci (Lond) 103:475-85. 2002
    ..UDC and TUDC can directly suppress disruption of mitochondrial membrane structure, which may represent an important mechanism of hepatoprotection by these bile salts...
  17. ncbi request reprint Functional modulation of nuclear steroid receptors by tauroursodeoxycholic acid reduces amyloid beta-peptide-induced apoptosis
    Susana Sola
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Lisbon 1600 083, Portugal
    Mol Endocrinol 20:2292-303. 2006
    ..In conclusion, these results provide novel insights into the specific cellular mechanism of TUDCA antiapoptotic function against Abeta-induced apoptosis and suggest targets for potential therapeutic intervention...
  18. ncbi request reprint The bile acid tauroursodeoxycholic acid modulates phosphorylation and translocation of bad via phosphatidylinositol 3-kinase in glutamate-induced apoptosis of rat cortical neurons
    Rui E Castro
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Av das Forcas Armadas, 1600 083 Lisbon, Portugal
    J Pharmacol Exp Ther 311:845-52. 2004
    ..These data suggest that TUDCA may be beneficial in treating neurodegenerative disorders in which increased glutamate levels contribute to the pathogenesis of the disease...
  19. ncbi request reprint Hepatocyte apoptosis, expression of death receptors, and activation of NF-kappaB in the liver of nonalcoholic and alcoholic steatohepatitis patients
    Paulo S Ribeiro
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Lisbon 1600 083, Portugal
    Am J Gastroenterol 99:1708-17. 2004
    ..In this study, we identified mechanisms of apoptosis induction in human steatohepatitis, and evaluated potential associations between apoptosis, liver pathology, and clinical presentation in NASH and ASH...
  20. pmc Tauroursodeoxycholic acid prevents amyloid-beta peptide-induced neuronal death via a phosphatidylinositol 3-kinase-dependent signaling pathway
    Susana Sola
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Portugal
    Mol Med 9:226-34. 2003
    ..Together, these findings indicate that Abeta-induced apoptosis of cortical neurons proceeds through a Bax mitochondrial pathway. Further, the PI3K signaling cascade plays a role in regulating the anti-apoptotic effects of TUDCA...
  21. ncbi request reprint Apoptosis and Bcl-2 expression in the livers of patients with steatohepatitis
    Rita M Ramalho
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon and Department of Gastroenterology, Hospital of Santa Maria, Lisbon, Portugal
    Eur J Gastroenterol Hepatol 18:21-9. 2006
    ..Apoptosis may play a role in the pathogenesis of alcoholic (ASH) and non-alcoholic steatohepatitis (NASH). In this study, we investigated the modulation of apoptosis-related liver proteins in steatohepatitis...
  22. pmc p53 interaction with JMJD3 results in its nuclear distribution during mouse neural stem cell differentiation
    Susana Sola
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    PLoS ONE 6:e18421. 2011
    ..This suggests that JMJD3 and p53 act in a common pathway during neurogenesis...
  23. doi request reprint miR-34a/SIRT1/p53 is suppressed by ursodeoxycholic acid in the rat liver and activated by disease severity in human non-alcoholic fatty liver disease
    Rui E Castro
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    J Hepatol 58:119-25. 2013
    ..Here, we aimed at evaluating the miR-34a/Sirtuin 1(SIRT1)/p53 pro-apoptotic pathway in human NAFLD, and to elucidate its function and modulation by UDCA in the rat liver and primary rat hepatocytes...
  24. ncbi request reprint p53 is a key molecular target of ursodeoxycholic acid in regulating apoptosis
    Joana D Amaral
    iMed UL, Faculty of Pharmacy, University of Lisbon, Avenida Prof Gama Pinto, Lisbon, Portugal
    J Biol Chem 282:34250-9. 2007
    ..In conclusion, these results further clarify the antiapoptotic mechanism of UDCA and suggest that modulation of Mdm-2/p53 interaction is a prime target for this bile acid...
  25. ncbi request reprint A distinct microarray gene expression profile in primary rat hepatocytes incubated with ursodeoxycholic acid
    Rui E Castro
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Lisbon 1600 083, Portugal
    J Hepatol 42:897-906. 2005
    ..However, precise pathways underlying UDCA's effects are not fully understood. The aim of this study was to identify specific cellular targets of UDCA...
  26. doi request reprint c-Jun regulates the stability of anti-apoptotic ΔNp63 in amyloid-β-induced apoptosis
    Maria B Fonseca
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    J Alzheimers Dis 28:685-94. 2012
    ....
  27. doi request reprint Tauroursodeoxycholic acid suppresses amyloid β-induced synaptic toxicity in vitro and in APP/PS1 mice
    Rita M Ramalho
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    Neurobiol Aging 34:551-61. 2013
    ..Taken together, these results expand the neuroprotective role of TUDCA to a synaptic level, further supporting the use of this molecule as a potential therapeutic strategy for the prevention and treatment of AD...
  28. doi request reprint MicroRNA-143 reduces viability and increases sensitivity to 5-fluorouracil in HCT116 human colorectal cancer cells
    Pedro M Borralho
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    FEBS J 276:6689-700. 2009
    ..Collectively, the data obtained in the present study suggest anti-proliferative, chemosensitizer and putative pro-apoptotic roles for miR-143 in colon cancer...
  29. ncbi request reprint Differential regulation of cyclin D1 and cell death by bile acids in primary rat hepatocytes
    Rui E Castro
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Av das Forcas Armadas, 1600 083 Lisbon, Portugal
    Am J Physiol Gastrointest Liver Physiol 293:G327-34. 2007
    ..In conclusion, cyclin D1 is a relevant player in modulating apoptosis by bile acids, in part through a p53-dependent mechanism...
  30. pmc Distinct regulatory functions of calpain 1 and 2 during neural stem cell self-renewal and differentiation
    Daniela M Santos
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    PLoS ONE 7:e33468. 2012
    ..Calpain 2 silencing elicited decreased levels of GFAP. These results support a role for calpain 1 in repressing differentiation, thus maintaining a proliferative NSC pool, and suggest that calpain 2 is involved in glial differentiation...
  31. pmc Apoptosis-associated microRNAs are modulated in mouse, rat and human neural differentiation
    Marcia M Aranha
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon 1649 003, Portugal
    BMC Genomics 11:514. 2010
    ..In addition, the expression of antiapoptotic miR-19a and 20a was also evaluated...
  32. ncbi request reprint Amyloid β peptides promote autophagy-dependent differentiation of mouse neural stem cells: Aβ-mediated neural differentiation
    Maria B Fonseca
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    Mol Neurobiol 48:829-40. 2013
    ..These results support distinct roles for different Aβ peptides in NSC fate decision and underline the importance of autophagy control of this process...
  33. pmc c-Jun N-terminal kinase 1/c-Jun activation of the p53/microRNA 34a/sirtuin 1 pathway contributes to apoptosis induced by deoxycholic acid in rat liver
    Duarte M S Ferreira
    Instituto de Investigação do Medicamento iMed ULisboa, Faculdade de Farmacia, Universidade de Lisboa, Lisbon, Portugal
    Mol Cell Biol 34:1100-20. 2014
    ..These results suggest that the JNK1/p53/miR-34a/SIRT1 pathway may represent an attractive pharmacological target for the development of new drugs to arrest metabolism- and apoptosis-related liver pathologies. ..
  34. doi request reprint Synthetic condensed 1,4-naphthoquinone derivative shifts neural stem cell differentiation by regulating redox state
    Daniela M Santos
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    Mol Neurobiol 47:313-24. 2013
    ..Collectively, these data support a new role for a specific naphthoquinone derivative in NSC fate decision and underline the importance of redox environment control...
  35. doi request reprint TUDCA, a bile acid, attenuates amyloid precursor protein processing and amyloid-β deposition in APP/PS1 mice
    Ana F Nunes
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, 1649 003, Portugal
    Mol Neurobiol 45:440-54. 2012
    ..These results highlight TUDCA as a potential therapeutic strategy for the prevention and treatment of AD...
  36. ncbi request reprint Inhibition of Fas expression by RNAi modulates 5-fluorouracil-induced apoptosis in HCT116 cells expressing wild-type p53
    Pedro M Borralho
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Portugal
    Biochim Biophys Acta 1772:40-7. 2007
    ..001). In conclusion, 5-FU exerts its cytotoxic effects, in part, through a p53/Fas-dependent apoptotic pathway that involves Bax translocation and mitochondrial permeabilization...
  37. ncbi request reprint Cell death targets and potential modulators in Alzheimer's disease
    Rui E Castro
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Av Prof Gama Pinto, 1649 003 Lisbon, Portugal
    Curr Pharm Des 16:2851-64. 2010
    ..This review focuses on molecular mechanisms of apoptosis in Alzheimer's disease and highlights the potential use of small molecule modulators to treat neurodegenerative disorders...
  38. doi request reprint Apoptosis inducing activity of benzophenanthridine-type alkaloids and 2-arylbenzofuran neolignans in HCT116 colon carcinoma cells
    Tayyab A Mansoor
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Av Prof Gama Pinto, 1649 003 Lisboa, Portugal
    Phytomedicine 20:923-9. 2013
    ..These results suggested that specific benzophenanthridine alkaloids and 2-arylbenzofuran neolignans isolated from Zanthoxylum capense show strong anticancer activity in HCT116 colon carcinoma cells...
  39. doi request reprint Monoterpene bisindole alkaloids, from the African medicinal plant Tabernaemontana elegans, induce apoptosis in HCT116 human colon carcinoma cells
    Tayyab A Mansoor
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisboa, Portugal
    J Ethnopharmacol 149:463-70. 2013
    ..The aims of the present study were to identify anti-cancer compounds, namely apoptosis inducers, from Tabernaemontana elegans, and hence to validate its usage in traditional medicine...
  40. doi request reprint Amyloid-β peptide-induced secretion of endoplasmic reticulum chaperone glycoprotein GRP94
    Ricardo J S Viana
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    J Alzheimers Dis 27:61-73. 2011
    ..Further characterization of this signaling pathway is likely to provide new perspectives for modulation of amyloid-induced apoptosis...
  41. pmc Identification of microRNAs during rat liver regeneration after partial hepatectomy and modulation by ursodeoxycholic acid
    Rui E Castro
    Research Institute for Medicines and Pharmaceutical Sciences, Univ of Lisbon, Portugal
    Am J Physiol Gastrointest Liver Physiol 299:G887-97. 2010
    ..miR-21 is additionally induced by UDCA in both regenerating rat liver and in vitro, which may represent a new mechanism behind UDCA biological functions...
  42. pmc Apoptosis in transgenic mice expressing the P301L mutated form of human tau
    Rita M Ramalho
    iMed UL, Faculty of Pharmacy, University of Lisbon, Av Prof Gama Pinto, Lisbon, Portugal
    Mol Med 14:309-17. 2008
    ..In conclusion, caspase-3-cleaved intermediate tau species occurred early in rTg54510 brains and preceded cell loss in Abeta-exposed cultured neurons. These results suggest a potential role of apoptosis in neurodegeneration...
  43. ncbi request reprint Nuclear translocation of UDCA by the glucocorticoid receptor is required to reduce TGF-beta1-induced apoptosis in rat hepatocytes
    Susana Sola
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    Hepatology 42:925-34. 2005
    ..In conclusion, these results further clarify the anti-apoptotic mechanism(s) of UDCA and suggest that GR is crucial for the nuclear translocation of this bile acid for reducing apoptosis...
  44. doi request reprint Ursodeoxycholic acid modulates the ubiquitin-proteasome degradation pathway of p53
    Joana D Amaral
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon 1649 003, Portugal
    Biochem Biophys Res Commun 400:649-54. 2010
    ..These findings suggest that UDCA protects cells from p53-mediated apoptosis by promoting its degradation via the Mdm-2-ubiquitin-proteasome pathway...
  45. ncbi request reprint Rat cultured neuronal and glial cells respond differently to toxicity of unconjugated bilirubin
    Rui F M Silva
    Centro de Patogenese Molecular, Faculdade de Farmacia, University of Lisbon, Lisbon, Portugal
    Pediatr Res 51:535-41. 2002
    ..These results suggest that UCB is toxic to both astrocytes and neurons, although through distinct pathways...
  46. ncbi request reprint Caspases and p53 modulate FOXO3A/Id1 signaling during mouse neural stem cell differentiation
    Marcia M Aranha
    iMed UL, University of Lisbon, Portugal
    J Cell Biochem 107:748-58. 2009
    ..In conclusion, apoptosis-associated factors such as caspases and p53 temporally modulate FOXO3A/Id1 signaling and differentiation of mouse NSCs...
  47. doi request reprint Dibenzylbutane- and butyrolactone-type lignans as apoptosis inducers in human hepatoma HuH-7 cells
    Tayyab A Mansoor
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Av Prof Gama Pinto, 1649 003, Lisbon, Portugal
    Phytother Res 26:692-6. 2012
    ..However, lignans 2 and 7 were the most promising compounds in this study, inducing 2.4- and 2.5-fold increases in apoptotic cells as compared to controls. Caspase-3-like activity assays confirmed the morphologic data...
  48. ncbi request reprint Plasma levels of ursodeoxycholic acid in black bears, Ursus americanus: seasonal changes
    Susana Sola
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Portugal
    Comp Biochem Physiol C Toxicol Pharmacol 143:204-8. 2006
    ..Further, the finding of seasonal variation in plasma bile acid composition provides evidence to support the possible role that ursodeoxycholic acid may play in cellular protection in hibernating black bears...
  49. doi request reprint Cytotoxic bile acids, but not cytoprotective species, inhibit the ordering effect of cholesterol in model membranes at physiologically active concentrations
    João Mello-Vieira
    Centro de Quimica Fisica Molecular and Institute of Nanoscience and Nanotechnology, IST, UTL, Lisbon, Portugal
    Biochim Biophys Acta 1828:2152-63. 2013
    ..g. modulation of lipid domains) or with an increase in mitochondrial membrane affinity for apoptotic proteins. ..
  50. pmc Liver and muscle in morbid obesity: the interplay of fatty liver and insulin resistance
    Mariana Verdelho Machado
    Departamento de Gastrenterologia, Unidade de Nutrição e Metabolismo, Hospital Santa Maria, Faculdade de Medicina de Lisboa, IMM, Lisbon, Portugal
    PLoS ONE 7:e31738. 2012
    ..We aimed to correlate muscle morphology, mitochondrial function and insulin signaling with NAFLD severity in morbid obese patients...
  51. ncbi request reprint No evidence of direct binding between ursodeoxycholic acid and the p53 DNA-binding domain
    Joana D Amaral
    Faculty of Pharmacy, Research Institute for Medicines and Pharmaceutical Sciences, University of Lisbon, Lisbon, Portugal
    Biosci Rep 30:359-64. 2010
    ..Our in vitro analysis did not provide any evidence for direct binding between the bile acid UDCA and the p53 core domain...
  52. ncbi request reprint Ursodeoxycholic acid modulates E2F-1 and p53 expression through a caspase-independent mechanism in transforming growth factor beta1-induced apoptosis of rat hepatocytes
    Susana Sola
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, 1600 083 Lisbon, Portugal
    J Biol Chem 278:48831-8. 2003
    ..05). In conclusion, these results demonstrate that UDCA inhibits E2F-1 transcriptional activation of hepatocyte apoptosis, thus modulating p53 stabilization, NF-kappaB degradation, and expression of Bcl-2 family members...
  53. doi request reprint Naphtho[2,3-d]isoxazole-4,9-dione-3-carboxylates: potent, non-cytotoxic, antiapoptotic agents
    Daniela M Santos
    iMed UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    Chem Biol Interact 180:175-82. 2009
    ..Our results suggest that naphtho[2,3-d]isoxazole-4,9-dione-3-carboxylates 1a and 1b may act as potent, cytoprotective agents, through modulation of apoptotic pathways...
  54. doi request reprint Modulation of amyloid-β peptide-induced toxicity through inhibition of JNK nuclear localization and caspase-2 activation
    Ricardo J S Viana
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    J Alzheimers Dis 22:557-68. 2010
    ..Further characterization of this signaling pathway and exact targets of modulation are likely to provide new perspectives for modulation of amyloid-induced apoptosis by TUDCA...
  55. ncbi request reprint Organelle stress sensors and cell death mechanisms in neurodegenerative diseases
    Ricardo J S Viana
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Av Prof Gama Pinto, 1649 003 Lisbon, Portugal
    CNS Neurol Disord Drug Targets 9:679-92. 2010
    ..The challenge is to decode the complex signaling network of inter-organellar crosstalk leading to cell death and identify therapeutic approaches for delaying or preventing neurodegenerative diseases...
  56. doi request reprint Death receptors and mitochondria: two prime triggers of neural apoptosis and differentiation
    Susana Sola
    Research Institute for Medicines and Pharmaceutical Sciences, Lisbon, Portugal
    Biochim Biophys Acta 1830:2160-6. 2013
    ....
  57. doi request reprint Bile acids and apoptosis modulation: an emerging role in experimental Alzheimer's disease
    Rita M Ramalho
    iMed UL, Faculty of Pharmacy, University of Lisbon, Av Prof Gama Pinto, 1649 003 Lisbon, Portugal
    Trends Mol Med 14:54-62. 2008
    ..In addition, TUDCA is capable of inducing survival pathways. Here, we review the role of apoptosis in AD and discuss the therapeutic potential of TUDCA in treating this disease...
  58. ncbi request reprint Targeting the p53 pathway of apoptosis
    Joana D Amaral
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Av Prof Gama Pinto, 1649 003 Lisbon, Portugal
    Curr Pharm Des 16:2493-503. 2010
    ..We will also review recent therapeutic strategies and clinical applications of targeted agents, and their progress in drug lead discovery, with particular emphasis on the potential use of UDCA...
  59. pmc Bile acids: regulation of apoptosis by ursodeoxycholic acid
    Joana D Amaral
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    J Lipid Res 50:1721-34. 2009
    ....
  60. ncbi request reprint Game and players: mitochondrial apoptosis and the therapeutic potential of ursodeoxycholic acid
    Susana Sola
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    Curr Issues Mol Biol 9:123-38. 2007
    ..Further, we address multiple interesting targets as well as the potential application of UDCA as a therapeutic modality for apoptosis-related disorders...
  61. doi request reprint Driving apoptosis-relevant proteins toward neural differentiation
    Susana Sola
    Research Institute for Medicines and Pharmaceutical Sciences, University of Lisbon, Lisbon, Portugal
    Mol Neurobiol 46:316-31. 2012
    ..In addition, we discuss how apoptosis-regulatory proteins control the decision between differentiation, self-renewal, and cell death in neural stem cells, and how activity is restrained to prevent cell loss...
  62. doi request reprint p53 and the regulation of hepatocyte apoptosis: implications for disease pathogenesis
    Joana D Amaral
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    Trends Mol Med 15:531-41. 2009
    ..Finally, we review recent therapeutic strategies and clinical applications of targeted agents, with a particular emphasis on the potential use of UDCA...
  63. ncbi request reprint Synthesis and evaluation of spiroisoxazoline oxindoles as anticancer agents
    Carlos J A Ribeiro
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Av Prof Gama Pinto, 1649 003 Lisbon, Portugal
    Bioorg Med Chem 22:577-84. 2014
    ..Moreover, proof-of-concept was demonstrated by inhibition of the interaction between p53 and MDM2 in a live-cell bimolecular fluorescence complementation assay. ..
  64. pmc Histone deacetylase inhibition decreases cholesterol levels in neuronal cells by modulating key genes in cholesterol synthesis, uptake and efflux
    Maria João Nunes
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    PLoS ONE 8:e53394. 2013
    ....
  65. doi request reprint Endoplasmic reticulum enrollment in Alzheimer's disease
    Ricardo J S Viana
    Research Institute for Medicines and Pharmaceutical Sciences, University of Lisbon, Lisbon 1649 003, Portugal
    Mol Neurobiol 46:522-34. 2012
    ..Here, we detail the involvement of the ER and associated stress pathways in AD and discuss potential therapeutic strategies targeting ER stress...
  66. doi request reprint Isoflavones as apoptosis inducers in human hepatoma HuH-7 cells
    Tayyab A Mansoor
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    Phytother Res 25:1819-24. 2011
    ..The compounds tested showed higher apoptosis induction profiles in HuH-7 cells compared with the control. Caspase activity assays confirmed the apoptosis inducing activity of these flavonoids...
  67. ncbi request reprint Role of nuclear steroid receptors in apoptosis
    Joana D Amaral
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Av Prof Gama Pinto, 1649 003 Lisbon, Portugal
    Curr Med Chem 16:3886-902. 2009
    ..The next decade will almost certainly unveil the remarkable role of NSR in modulating cell fate in human health and disease...
  68. doi request reprint Bile acid levels are increased in the liver of patients with steatohepatitis
    Marcia M Aranha
    Centro de Patogenese Molecular, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    Eur J Gastroenterol Hepatol 20:519-25. 2008
    ..The aim of this study was to characterize bile acid profiles in liver tissue of patients with steatohepatitis...
  69. ncbi request reprint Dinitro-o-cresol induces apoptosis-like cell death but not alternative oxidase expression in soybean cells
    Marcia M Aranha
    Centro de Engenharia Biológica and Departamento de Biologia Vegetal, Faculty of Sciences, University of Lisbon, Campo Grande, 1749 016 Lisbon, Lisbon, Portugal
    J Plant Physiol 164:675-84. 2007
    ..Further, the role of AOX in cell death has not been confirmed, and may depend on the nature and intensity of stress conditions...
  70. ncbi request reprint Bilirubin-induced apoptosis in cultured rat neural cells is aggravated by chenodeoxycholic acid but prevented by ursodeoxycholic acid
    R F Silva
    , , University of Lisbon, Portugal
    J Hepatol 34:402-8. 2001
    ..CONCLUSIONS: UCB is toxic to both astrocytes and neurones, causing cell death through an apoptotic process. Moreover, UDCA inhibits UCB-induced apoptosis in neural cells and this could not be mimicked by other bile acids...
  71. doi request reprint Induction of apoptosis in HuH-7 cancer cells by monoterpene and beta-carboline indole alkaloids isolated from the leaves of Tabernaemontana elegans
    Tayyab A Mansoor
    iMed UL, Faculdade de Farmacia, Universidade de Lisboa, Av D Forças Armadas, 1600 083 Lisboa, Portugal
    Bioorg Med Chem Lett 19:4255-8. 2009
    ..The monoterpene indole alkaloids, tabernaemontanine (1) and vobasine (3) showed the most promising apoptosis induction profile in HuH-7 cells...
  72. doi request reprint Delivering the promise of miRNA cancer therapeutics
    Diane M Pereira
    Research Institute for Medicines and Pharmaceutical Sciences iMED UL, Faculty of Pharmacy, University of Lisbon, Av Prof Gama Pinto, 1649 003 Lisbon, Portugal
    Drug Discov Today 18:282-9. 2013
    ..We herein review in vivo strategies of miRNA modulator (mimic and/or inhibitor) delivery in cancer models, a subject that remains the key challenge to the establishment of this novel class of RNA therapeutics...