Research Topics
| Margarida LimaSummaryCountry: Portugal Publications
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Publications
Laboratory diagnosis of large granular lymphocytic leukemiaMargarida Lima
Service of Hematology, , Porto Portugal
Lab Hematol 10:148-9. 2004
Reactive phenotypes after acute and chronic NK-cell activationM Lima
Service of Clinical Hematology, Laboratory of Cytometry, Hospital Geral de Santo Antonio, Porto, Portugal
J Biol Regul Homeost Agents 18:331-4. 2004..Here, we review the reactive phenotypes observed in vivo after acute and chronic NK-cell activation...
Immunophenotypic analysis of the TCR-Vbeta repertoire in 98 persistent expansions of CD3(+)/TCR-alphabeta(+) large granular lymphocytes: utility in assessing clonality and insights into the pathogenesis of the diseaseM Lima
Serviço de Hematologia Clínica, Unidade de Citometria, Hospital Geral de Santo Antonio, Porto, Portugal
Am J Pathol 159:1861-8. 2001..In summary, our results suggest that flow cytometry immunophenotypic analysis of the TCR-Vbeta repertoire is a powerful screening tool for the assessment of T-cell clonality in persistent expansions of TCR-alphabeta(+) LGLs...
Clinicobiological, immunophenotypic, and molecular characteristics of monoclonal CD56-/+dim chronic natural killer cell large granular lymphocytosisMargarida Lima
Servico de Hematologia, Unidade de Citometria, Hospital Geral de Santo Antonio, Rua D Manuel II, S N, 4099 001 Porto, Portugal
Am J Pathol 165:1117-27. 2004....
Utility of flow cytometry immunophenotyping and DNA ploidy studies for diagnosis and characterization of blood involvement in CD4+ Sézary's syndromeMargarida Lima
Servico de Hematologia, Unidade de Citometria, Hospital Geral de Santo Antonio, Rua D Manuel II, S N, 4099 001 Porto, Portugal
Haematologica 88:874-87. 2003..The exact immunophenotypic criteria for the identification of Sézary cells in the blood are still poorly defined...
Non-cytotoxic gamma-delta peripheral T-cell lymphoma affecting the mandibular and parotidal lymph nodes and the skinMargarida Lima
Servico de Hematologia, Unidade de Citometria, Hospital Geral de Santo Antonio, Rua D Manuel II, s n 4099 001 Porto, Portugal
Leuk Lymphoma 44:525-9. 2003..The disease had an aggressive course and was resistant to chemotherapy and the patient died 4 months after diagnosis...
Immunophenotype and TCR-Vbeta repertoire of peripheral blood T-cells in acute infectious mononucleosisMargarida Lima
Service of Clinical Haematology, Hospital Santo Antonio, Porto, Portugal
Blood Cells Mol Dis 30:1-12. 2003..The results presented here provide evidence for an extensive T-cell activation during acute viral infection and establish the immunophenotype patterns associated with this condition...
Guess what: Chronic 13q14.3+/CD5-/CD23+ lymphocytic leukemia in blood and t(11;14)(q13;q32)+/CD5+/CD23- mantle cell lymphoma in lymph nodes!Margarida Lima
Service of Clinical Hematology, Hospital Geral de Santo Antonio, Porto, Portugal
Cytometry B Clin Cytom 51:41-4. 2003..The B-cell lymphocytic leukemia clone predominated in the blood and bone marrow, whereas the mantle cell clone predominated in lymph nodes...
Atopic dermatitis-like non-erythrodermic leukemic variant of CD3(-/+dim) CD4(+) cutaneous T-cell lymphoma preceded by cutaneous papular xanthomatosisMargarida Lima
Service of Clinical Hematology, Hospital Geral de Santo Antonio, Porto, Portugal
Leuk Lymphoma 45:597-603. 2004....
Association of CD4+/CD56+/CD57+/CD8+(dim) large granular lymphocytic leukemia, splenic B-cell lymphoma with circulating villous lymphocytes, and idiopathic erythrocytosisM Lima
Service of Clinical Hematology, Hospital Geral Santo Antonio, Porto, Portugal
Ann Hematol 80:685-90. 2001..We also discuss the possible reasons for this particular association...
Immunophenotypic characterization of normal blood CD56+lo versus CD56+hi NK-cell subsets and its impact on the understanding of their tissue distribution and functional propertiesM Lima
Service of Clinical Hematology, Unit of Cytometry, Hospital Geral de Santo Antonio, Porto, Portugal
Blood Cells Mol Dis 27:731-43. 2001....
Philadelphia-positive T-cell acute lymphoblastic leukemia with polymyositis, migratory polyarthritis and hypercalcemia following a chronic myeloid leukemiaM Lima
Department of Clinical Hematology, Hospital Geral de Santo Antonio, Rua D Manuel II, S N, 4099 001 Porto, Portugal
Ann Hematol 81:174-7. 2002..Polymyositis and polyarthritis preceded by 4 months, and symptomatic hypercalcemia occurred just before blastic transformation, probably representing paraneoplastic manifestations of the disease...
Aggressive natural-killer cell lymphoma presenting with skin lesions, breast nodule, suprarenal masses and life-threatening pericardial and pleural effusionsM Lima
Service of Clinical Hematology, Hospital Geral de Santo Antonio, Porto, Portugal
Leuk Lymphoma 42:1385-91. 2001..Death occurred 4 months after the first manifestations of the disease as a consequence of cardiac tamponade...
The "ex vivo" patterns of CD2/CD7, CD57/CD11c, CD38/CD11b, CD45RA/CD45RO, and CD11a/HLA-DR expression identify acute/early and chronic/late NK-cell activation statesMargarida Lima
Service of Clinical Hematology, Hospital Geral de Santo Antonio, Porto, Portugal
Blood Cells Mol Dis 28:181-90. 2002....
Intraclonal diversity in a Sezary syndrome with a differential response to 2-deoxycoformycin of the two lymphoma cell populationsElisa Granjo
Department of Clinical Haematology, Hospital Geral de São João, Porto, Portugal
Br J Haematol 119:629-33. 2002....
TCRalphabeta+/CD4+ large granular lymphocytosis: a new clonal T-cell lymphoproliferative disorderMargarida Lima
, Unidade de Citometria, Hospital Geral de Santo Antonio, Porto, Portugal
Am J Pathol 163:763-71. 2003..Longer follow-up periods are necessary to determine the exact significance of this clonal disorder...
Chemokine receptor repertoire reflects mature T-cell lymphoproliferative disorder clinical presentationJoão Moura
Laboratório de Citometria, Serviço de Hematologia Clínica, Centro Hospitalar do Porto CHP, Hospital Geral de Santo António HGSA, Porto, Portugal
Blood Cells Mol Dis 42:57-63. 2009..In addition, in the case of CD8 T-cell leukemia patients, an inverse correlation with neutropenia was found. In lymphoma patients, higher CCR4 and CCR7 expression is accompanied by lower to absent CCR5 expression...
Sezary syndrome presenting with leonine facies and treated with low-dose subcutaneous alemtuzumabAna Oliveira
Hospital de Santo Antonio, Centro Hospitalar do Porto, Porto, Portugal
Dermatol Online J 17:6. 2011..We present the case of a 54-year-old man with Sezary syndrome presenting with leonine facies, unresponsive to conventional therapies; he exhibited a promising response to subcutaneous low-dose alemtuzumab...
Granulomatous slack skinMarta Teixeira
Dept of Dermatology, Hospital Geral de Santo Antonio, Rua D Manuel II, Edificio ex Cicap, 4099 001 Porto, Portugal
Eur J Dermatol 17:435-8. 2007..Notwithstanding its indolent behavior, treatment is often disappointing. We describe an additional case of this rare disorder in a 44-year-old male patient...
Hematological remission and long term hematological control of acute myeloblastic leukemia induced and maintained by granulocyte-colony stimulating factor (G-CSF) therapyLuciana Xavier
Service of Clinical Hematology, Centro Hospitalar de Vila Nova de Gaia, Vila Nova de Gaia, Portugal
Leuk Lymphoma 44:2137-42. 2003....
Mutations in the beta3 gene giving rise to type I Glanzmann thrombasthenia in two families in PortugalLoida Corbillon Garcia
Hospital de Santo Antonio, , Porto, Portugal
Platelets 15:15-22. 2004..For both patients a single mutated allele was inherited from each parent. Evidence is accumulating that nonsense mutations leading to a truncated beta3 may be a frequent cause of type I Glanzmann thrombasthenia in the Iberian peninsula...
Letter: Primary cutaneous marginal zone B cell lymphoma of the face: A challenging diagnosisAna Oliveira
Department of Dermatology, Centro Hospitalar do Porto Hospital de Santo António, Porto, Portugal
Dermatol Online J 18:12. 2012..We present a case report of a man, 80 years of age, with a primary cutaneous marginal B cell lymphoma of the chin, an atypical location...
A nonsense porcn mutation in severe focal dermal hypoplasia with natal teethCristina Dias
Centro de Genética Médica Dr Jacito Magalhaes INSA, Genetica Medica, Porto, Portugal
Fetal Pediatr Pathol 29:305-13. 2010..Mutation analysis of PORCN revealed a nonsense mutation-Y359X. She presented natal teeth, an unexpected feature considering the role of the Wnt pathway in tooth development...
Abnormal NK cell lymphocytosis detected after splenectomy: association with repeated infections, relapsing neutropenia, and persistent polyclonal B-cell proliferationElisa Granjo
Department of Hematology, Hospital S Joao, Porto, Portugal
Int J Hematol 75:484-8. 2002....
Neonatal alloimmune neutropenia: still a diagnostic and therapeutical challengeSofia Agueda
Neonatal Intensive Care Unit, Hospital Sao Joao, EPE, Alameda Prof Hernani Monteiro, Porto, Portugal
J Pediatr Hematol Oncol 34:497-9. 2012..To describe a case of neonatal alloimmune neutropenia (NAN), a very rare disease of the newborn and the first ever reported in our neonatal intensive care unit, with emphasis in its management and outcome...
Neutrophil and monocyte activation in chronic kidney disease patients under hemodialysis and its relationship with resistance to recombinant human erythropoietin and to the hemodialysis procedureRui Pereira
Biochemistry Department, Faculty of Pharmacy, University of Porto, Porto, Portugal
Hemodial Int 14:295-301. 2010..Moreover, this activation is due, at least in part, to the HD procedure, although we should not exclude that it can also be due to the enhanced inflammatory process observed in nonresponder patients...
Inflammation, T-cell phenotype, and inflammatory cytokines in chronic kidney disease patients under hemodialysis and its relationship to resistance to recombinant human erythropoietin therapyElisio Costa
Serviço de Bioquímica, Faculdade de Farmacia da Universidade do Porto, Rua Anibal Cunha, 164, 4099 030 Porto, Portugal
J Clin Immunol 28:268-75. 2008....
Monoclonal TCR-Vbeta13.1+/CD4+/NKa+/CD8-/+dim T-LGL lymphocytosis: evidence for an antigen-driven chronic T-cell stimulation originPilar Garrido
Servicio de Hematologia, Hospital Universitario Virgen de las Nieves, Granada, Spain
Blood 109:4890-8. 2007....
Response to cyclosporine in a patient with disseminated granuloma annulare associated with CD4+/CD8+(dim)/CD56+ large granular lymphocytic leukemiaElisa Granjo
Arch Dermatol 138:274-6. 2002
Evaluation of functional single nucleotide polymorphisms of different genes coding for the immunoregulatory molecules in patients with monoclonal large granular lymphocyte lymphocytosisPilar Garrido
Servicio de Hematologia, Hospital Universitario Virgen de las Nieves, Granada, Spain
Hum Immunol 69:101-7. 2008..Our results suggest that the frequency of SNP of the genes coding for the studied immunoregulatory molecules are not associated with the development of CD4+/TCR alpha beta+ T-LGL...
Bilateral orbital masses in a patient with B-cell chronic lymphHenrique Coelho
Haematologica 88:ECR12. 2003
