Henrique Girao

Summary

Affiliation: Faculty of Medicine
Country: Portugal

Publications

  1. doi request reprint Eps15 interacts with ubiquitinated Cx43 and mediates its internalization
    Henrique Girao
    Centre of Ophthalmology and Visual Sciences, Biomedical Institute for Research in Light and Image IBILI, Faculty of Medicine, University of Coimbra, Azinhaga de Sta Comba 3000 354 Coimbra, Portugal
    Exp Cell Res 315:3587-97. 2009
  2. ncbi request reprint Phosphorylation of connexin 43 acts as a stimuli for proteasome-dependent degradation of the protein in lens epithelial cells
    Henrique Girao
    Center for Ophthalmology, Biomedical Institute for Research in Light and Image, Faculty of Medicine, University of Coimbra, Portugal
    Mol Vis 9:24-30. 2003
  3. pmc Subcellular redistribution of components of the ubiquitin-proteasome pathway during lens differentiation and maturation
    Henrique Girao
    Center of Ophthalmology, Biomedical Institute for Research in Light and Image, University of Coimbra, Portugal
    Invest Ophthalmol Vis Sci 46:1386-92. 2005
  4. doi request reprint Ubiquitin-mediated internalization of connexin43 is independent of the canonical endocytic tyrosine-sorting signal
    Steve Catarino
    Centre of Ophthalmology and Vision Sciences, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
    Biochem J 437:255-67. 2011
  5. ncbi request reprint The proteasome regulates the interaction between Cx43 and ZO-1
    Henrique Girao
    Centre of Ophthalmology, Biomedical Institute for Research in Light and Image IBILI, Faculty of Medicine, University of Coimbra, 3000 354 Coimbra, Portugal
    J Cell Biochem 102:719-28. 2007
  6. doi request reprint Heart ischemia results in connexin43 ubiquitination localized at the intercalated discs
    Tânia Martins-Marques
    Centre of Ophthalmology and Vision Sciences, Institute of Biomedical Imaging and Life Sciences IBILI, Faculty of Medicine, University of Coimbra, Portugal Electronic address
    Biochimie 112:196-201. 2015
  7. doi request reprint AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions
    Teresa M Ribeiro-Rodrigues
    Centre of Ophthalmology and Vision Sciences, Institute of Biomedical Imaging and Life Sciences IBILI, Faculty of Medicine, and Centro de Física Computacional CFC, Departamento de Fisica, University of Coimbra, Coimbra, Portugal
    FASEB J 28:4629-41. 2014
  8. ncbi request reprint 7-ketocholesterol stimulates differentiation of lens epithelial cells
    Henrique Girao
    Centre of Ophthalmology, Biomedical Institute for Research in Light and Image IBILI, Faculty of Medicine, University of Coimbra, Portugal
    Mol Vis 9:497-501. 2003
  9. doi request reprint Ischaemia-induced autophagy leads to degradation of gap junction protein connexin43 in cardiomyocytes
    Tânia Martins-Marques
    Centre of Ophthalmology and Vision Sciences, Institute of Biomedical Imaging and Life Sciences IBILI, Faculty of Medicine, University of Coimbra, Azinhaga de Sta Comba, 3000 354 Coimbra, Portugal
    Biochem J 467:231-45. 2015
  10. ncbi request reprint Cholesterol oxides mediated changes in cytoskeletal organisation involves Rho GTPases small star, filled
    Henrique Girao
    Centre of Ophthalmology, Biomedical Institute for Research in Light and Image IBILI, Faculty of Medicine, University of Coimbra, 3000 Coimbra, Portugal
    Exp Cell Res 291:502-13. 2003

Collaborators

  • Paulo Pereira
  • Fu Shang
  • Allen Taylor
  • Rosa Fernandes
  • Tânia Martins-Marques
  • Carla Marques
  • Steve Catarino
  • Paulo Matafome
  • Joana Balça-Silva
  • Teresa Ribeiro-Rodrigues
  • Maria Celeste Lopes
  • Joana Liberal
  • Teresa M Ribeiro-Rodrigues
  • Diana Matias
  • Patrice Codogno
  • Monica Zuzarte
  • Vivaldo Moura-Neto
  • Anália do Carmo
  • Ana Bela Sarmento-Ribeiro
  • Rui Baptista
  • Maria Teresa Cruz
  • João V Ferreira
  • Vera Francisco
  • Artur Figueirinha
  • Maria Teresa Amaral
  • Maria Teresa Batista
  • Gustavo Costa
  • Jose S Ramalho

Detail Information

Publications15

  1. doi request reprint Eps15 interacts with ubiquitinated Cx43 and mediates its internalization
    Henrique Girao
    Centre of Ophthalmology and Visual Sciences, Biomedical Institute for Research in Light and Image IBILI, Faculty of Medicine, University of Coimbra, Azinhaga de Sta Comba 3000 354 Coimbra, Portugal
    Exp Cell Res 315:3587-97. 2009
    ..This provides the basis for future studies aiming at identifying the molecular players and mechanisms involved in Cx43 internalization and degradation...
  2. ncbi request reprint Phosphorylation of connexin 43 acts as a stimuli for proteasome-dependent degradation of the protein in lens epithelial cells
    Henrique Girao
    Center for Ophthalmology, Biomedical Institute for Research in Light and Image, Faculty of Medicine, University of Coimbra, Portugal
    Mol Vis 9:24-30. 2003
    ..The objective of this report is to determine whether phosphorylation of Cx43 in lens epithelial cells alters its resistance to degradation by a proteasome dependent mechanism...
  3. pmc Subcellular redistribution of components of the ubiquitin-proteasome pathway during lens differentiation and maturation
    Henrique Girao
    Center of Ophthalmology, Biomedical Institute for Research in Light and Image, University of Coimbra, Portugal
    Invest Ophthalmol Vis Sci 46:1386-92. 2005
    ..The primary nuclear localization of the UPP components in the differentiating fibers supports the hypothesis that the UPP may play a role in elimination of nuclei and other organelles during differentiation and maturation of lens fibers...
  4. doi request reprint Ubiquitin-mediated internalization of connexin43 is independent of the canonical endocytic tyrosine-sorting signal
    Steve Catarino
    Centre of Ophthalmology and Vision Sciences, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
    Biochem J 437:255-67. 2011
    ..Furthermore, the internalization of Cx43 induced by Cx43-Ub was shown to depend on its interaction with epidermal growth factor receptor substrate 15...
  5. ncbi request reprint The proteasome regulates the interaction between Cx43 and ZO-1
    Henrique Girao
    Centre of Ophthalmology, Biomedical Institute for Research in Light and Image IBILI, Faculty of Medicine, University of Coimbra, 3000 354 Coimbra, Portugal
    J Cell Biochem 102:719-28. 2007
    ..Data presented in this article suggest a new pathway whereby alterations in proteasome activity may impact on GJIC as well as on non-junctional communication with extracellular environment, contributing to cell and tissue dysfunction...
  6. doi request reprint Heart ischemia results in connexin43 ubiquitination localized at the intercalated discs
    Tânia Martins-Marques
    Centre of Ophthalmology and Vision Sciences, Institute of Biomedical Imaging and Life Sciences IBILI, Faculty of Medicine, University of Coimbra, Portugal Electronic address
    Biochimie 112:196-201. 2015
    ..Altogether, these results suggest that ubiquitin is involved in the remodeling of GJ during myocardial ischemia, which requires the recruitment of Nedd4. ..
  7. doi request reprint AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions
    Teresa M Ribeiro-Rodrigues
    Centre of Ophthalmology and Vision Sciences, Institute of Biomedical Imaging and Life Sciences IBILI, Faculty of Medicine, and Centro de Física Computacional CFC, Departamento de Fisica, University of Coimbra, Coimbra, Portugal
    FASEB J 28:4629-41. 2014
    ..Overall, these data strongly suggest that AMSH-mediated deubiquitination of Cx43 protects GJs from degradation...
  8. ncbi request reprint 7-ketocholesterol stimulates differentiation of lens epithelial cells
    Henrique Girao
    Centre of Ophthalmology, Biomedical Institute for Research in Light and Image IBILI, Faculty of Medicine, University of Coimbra, Portugal
    Mol Vis 9:497-501. 2003
    ..To establish if oxysterols stimulate differentiation of lens epithelial cells (LEC)...
  9. doi request reprint Ischaemia-induced autophagy leads to degradation of gap junction protein connexin43 in cardiomyocytes
    Tânia Martins-Marques
    Centre of Ophthalmology and Vision Sciences, Institute of Biomedical Imaging and Life Sciences IBILI, Faculty of Medicine, University of Coimbra, Azinhaga de Sta Comba, 3000 354 Coimbra, Portugal
    Biochem J 467:231-45. 2015
    ....
  10. ncbi request reprint Cholesterol oxides mediated changes in cytoskeletal organisation involves Rho GTPases small star, filled
    Henrique Girao
    Centre of Ophthalmology, Biomedical Institute for Research in Light and Image IBILI, Faculty of Medicine, University of Coimbra, 3000 Coimbra, Portugal
    Exp Cell Res 291:502-13. 2003
    ....
  11. ncbi request reprint High glucose down-regulates intercellular communication in retinal endothelial cells by enhancing degradation of connexin 43 by a proteasome-dependent mechanism
    Rosa Fernandes
    Centre of Ophthalmology, Biomedical Institute for Research in Light and Image, Faculty of Medicine, University of Coimbra, 3000 354 Coimbra, Portugal
    J Biol Chem 279:27219-24. 2004
    ..Increased degradation of Cx43 and reduction of GJIC in high glucose may be of physiological importance by contributing to endothelial cell dysfunction associated with the breakdown of the blood-retinal barrier in diabetic retinopathy...
  12. pmc Autophagy and ubiquitination in cardiovascular diseases
    Tânia Martins-Marques
    1 Centre of Ophthalmology and Vision Sciences, Institute of Biomedical Imaging and Life Sciences IBILI, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
    DNA Cell Biol 34:243-51. 2015
    ..In this review, we present recent studies devoted to the involvement of autophagy in heart homeostasis, with a particular focus on ubiquitin and GJs. ..
  13. doi request reprint Bioactivity of Fragaria vesca leaves through inflammation, proteasome and autophagy modulation
    Joana Liberal
    Center for Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000 548 Coimbra, Portugal Center for Neurosciences and Cell Biology, University of Coimbra, Largo Marques de Pombal, 3004 517 Coimbra, Portugal Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000 548 Coimbra, Portugal
    J Ethnopharmacol 158:113-22. 2014
    ..This work aims to disclose the bioactivity and the underlying action mechanism of an extract from Fragaria vesca leaves in order to support its traditional uses...
  14. doi request reprint Tamoxifen in combination with temozolomide induce a synergistic inhibition of PKC-pan in GBM cell lines
    Joana Balça-Silva
    Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal Faculty of Medicine, University of Coimbra, Coimbra, Portugal Electronic address
    Biochim Biophys Acta 1850:722-32. 2015
    ..Here we describe the potential synergistic behavior of the therapeutic combination of tamoxifen (TMX), a known inhibitor of PKC, and TMZ in GBM...