Thomas Hanscheid

Summary

Affiliation: Faculdade de Medicina
Country: Portugal

Publications

  1. ncbi request reprint How useful is PCR in the diagnosis of malaria?
    Thomas Hanscheid
    Laboratorio de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Hospital de Santa Maria, 1600 Lisboa, Portugal
    Trends Parasitol 18:395-8. 2002
  2. pmc Simple flow cytometric detection of haemozoin containing leukocytes and erythrocytes for research on diagnosis, immunology and drug sensitivity testing
    Rosangela Frita
    Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Lisbon, Hospital Universitario de Santa Maria, Av, Prof, Egas Moniz, P 1649 028 Lisboa, Portugal
    Malar J 10:74. 2011
  3. pmc Automated haematology analysis to diagnose malaria
    Germán Campuzano-Zuluaga
    Grupo Malaria, Facultad de Medicina, Universidad de Antioquia, Calle 62 52 59, Lab 610, Medellin, Colombia
    Malar J 9:346. 2010
  4. doi request reprint The future looks bright: low-cost fluorescent microscopes for detection of Mycobacterium tuberculosis and Coccidiae
    Thomas Hanscheid
    Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Lisboa, Portugal
    Trans R Soc Trop Med Hyg 102:520-1. 2008
  5. pmc Full blood count and haemozoin-containing leukocytes in children with malaria: diagnostic value and association with disease severity
    Thomas Hanscheid
    Medical Research Unit, Albert Schweitzer Hospital, Lambarene, Gabon
    Malar J 7:109. 2008
  6. ncbi request reprint Screening of auramine-stained smears of all fecal samples is a rapid and inexpensive way to increase the detection of coccidial infections
    T Hanscheid
    Unidade de Microbiologia Molecular e Infecção, Instituto de Medicina Molecular, Faculdade de Medicina, Hospital Universitario de Santa Maria, Av Prof Egas Moniz, P 1649 028 Lisbon, Portugal
    Int J Infect Dis 12:47-50. 2008
  7. pmc Growth of Mycobacterium tuberculosis in conventional BacT/ALERT FA blood culture bottles allows reliable diagnosis of Mycobacteremia
    T Hanscheid
    Laboratório de Basteriologia, Hospital de Santa Maria, Lisbon, Portugal
    J Clin Microbiol 43:890-1. 2005
  8. ncbi request reprint Current strategies to avoid misdiagnosis of malaria
    T Hanscheid
    Laboratorio de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Hospital de Santa Maria, Lisboa, Portugal
    Clin Microbiol Infect 9:497-504. 2003
  9. ncbi request reprint Feasibility of introducing rejection criteria for stool cultures in a teaching hospital in Portugal
    T Hanscheid
    Laboratorio de Bacteriologia, Hospital Santa Maria, 1600 Lisboa, Portugal
    Clin Microbiol Infect 8:118-21. 2002
  10. ncbi request reprint Automated detection of malaria pigment in white blood cells for the diagnosis of malaria in Portugal
    T Hanscheid
    Department of Clinical Pathology, Hospital Santa Maria, Lisbon, Portugal
    Am J Trop Med Hyg 64:290-2. 2001

Collaborators

Detail Information

Publications24

  1. ncbi request reprint How useful is PCR in the diagnosis of malaria?
    Thomas Hanscheid
    Laboratorio de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Hospital de Santa Maria, 1600 Lisboa, Portugal
    Trends Parasitol 18:395-8. 2002
    ..Even in affluent, non-endemic countries, PCR is not a suitable method for routine use. Nonetheless, PCR could be clinically useful in selected situations...
  2. pmc Simple flow cytometric detection of haemozoin containing leukocytes and erythrocytes for research on diagnosis, immunology and drug sensitivity testing
    Rosangela Frita
    Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Lisbon, Hospital Universitario de Santa Maria, Av, Prof, Egas Moniz, P 1649 028 Lisboa, Portugal
    Malar J 10:74. 2011
    ..These can range from the ex-vivo and in-vitro detection and functional analysis of Hz-containing leukocytes to the detection of parasitized Red-Blood-Cells (pRBCs) to assess antimalarial activity...
  3. pmc Automated haematology analysis to diagnose malaria
    Germán Campuzano-Zuluaga
    Grupo Malaria, Facultad de Medicina, Universidad de Antioquia, Calle 62 52 59, Lab 610, Medellin, Colombia
    Malar J 9:346. 2010
    ....
  4. doi request reprint The future looks bright: low-cost fluorescent microscopes for detection of Mycobacterium tuberculosis and Coccidiae
    Thomas Hanscheid
    Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Lisboa, Portugal
    Trans R Soc Trop Med Hyg 102:520-1. 2008
    ..Based on LEDs, rather inexpensive solutions for fluorescent microscopes are now available and thus auramine may be a cost-effective step to improve the diagnosis of tuberculosis in resource-poor countries...
  5. pmc Full blood count and haemozoin-containing leukocytes in children with malaria: diagnostic value and association with disease severity
    Thomas Hanscheid
    Medical Research Unit, Albert Schweitzer Hospital, Lambarene, Gabon
    Malar J 7:109. 2008
    ..Diligent and correct laboratory diagnosis and up-front identification of risk factors for progression to severe disease are the basis for optimal management of malaria...
  6. ncbi request reprint Screening of auramine-stained smears of all fecal samples is a rapid and inexpensive way to increase the detection of coccidial infections
    T Hanscheid
    Unidade de Microbiologia Molecular e Infecção, Instituto de Medicina Molecular, Faculdade de Medicina, Hospital Universitario de Santa Maria, Av Prof Egas Moniz, P 1649 028 Lisbon, Portugal
    Int J Infect Dis 12:47-50. 2008
    ..Reappraisal and understanding of the accurate staining characteristics of auramine O (AuO), which stains nucleic acids, may permit the inexpensive and reliable identification of coccidian oocysts at routine workup of all fecal samples...
  7. pmc Growth of Mycobacterium tuberculosis in conventional BacT/ALERT FA blood culture bottles allows reliable diagnosis of Mycobacteremia
    T Hanscheid
    Laboratório de Basteriologia, Hospital de Santa Maria, Lisbon, Portugal
    J Clin Microbiol 43:890-1. 2005
    ..tuberculosis from blood in HIV-infected patients. Overall, blood cultures were positive in 39% of patients with tuberculosis...
  8. ncbi request reprint Current strategies to avoid misdiagnosis of malaria
    T Hanscheid
    Laboratorio de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Hospital de Santa Maria, Lisboa, Portugal
    Clin Microbiol Infect 9:497-504. 2003
    ..The detection of intraleukocytic hemozoin during automated full blood counts is a promising new way to avoid misdiagnosis of clinically unsuspected malaria...
  9. ncbi request reprint Feasibility of introducing rejection criteria for stool cultures in a teaching hospital in Portugal
    T Hanscheid
    Laboratorio de Bacteriologia, Hospital Santa Maria, 1600 Lisboa, Portugal
    Clin Microbiol Infect 8:118-21. 2002
    ..Only in three cases would previously proposed exceptions to the rejection criteria have applied. A '5-day-rule' and <or= 2 specimen/patients seem to be more suitable, based on the different hospital conditions present here...
  10. ncbi request reprint Automated detection of malaria pigment in white blood cells for the diagnosis of malaria in Portugal
    T Hanscheid
    Department of Clinical Pathology, Hospital Santa Maria, Lisbon, Portugal
    Am J Trop Med Hyg 64:290-2. 2001
    ..This instrument appears to provide a promising method for the diagnosis of malaria, especially where automated full blood counts are routine in the work-up of febrile patients...
  11. pmc Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy?
    Thomas Hanscheid
    Medical Research Unit, Hopital Albert Schweitzer, Lambarene, Gabon
    Malar J 8:255. 2009
    ..Detection of malaria pigment (or haemozoin; Hz)-containing leukocytes may have prognostic relevance in malaria; however, studies reported conflicting results, with microscopic counts suggestive of being inaccurate and imprecise...
  12. ncbi request reprint Avoiding misdiagnosis of imported malaria: screening of emergency department samples with thrombocytopenia detects clinically unsuspected cases
    Thomas Hanscheid
    Instituto de Medicina Molecular, Faculdade de Medicina, and Patologia Clinica, Hospital Santa Maria, Lisboa, Portugal
    J Travel Med 10:155-9. 2003
    ..However, blind screening of all thrombocytopenic samples might be a possible way to detect clinically unsuspected malaria cases in the accident and emergency department (AED)...
  13. doi request reprint Automated detection of haemozoin-containing monocytes for the diagnosis of malaria in microscopically negative cases during pregnancy
    Thomas Hanscheid
    Medical Research Unit, Albert Schweitzer Hospital, Lambarene, Gabon
    Acta Trop 109:245-6. 2009
    ..In a subset of 37 Cell-Dyn positive but microscopy negative samples, PCR detected five positive cases. This methodology may serve as an adjunct rapid diagnostic tool for malaria during pregnancy, even in microscopically negative cases...
  14. doi request reprint Haemozoin detection in infected erythrocytes for Plasmodium falciparum malaria diagnosis-prospects and limitations
    Maria Rebelo
    Instituto de Medicina Molecular, Faculdade de Medicina, Lisbon, Portugal
    Acta Trop 123:58-61. 2012
    ..Our results strongly suggest that, although it may be useful for monitoring maturation, detection of intra-erythrocytic Hz by flow cytometry will not provide an optimal method for diagnosis of P. falciparum malaria...
  15. ncbi request reprint Haemozoin: from melatonin pigment to drug target, diagnostic tool, and immune modulator
    Thomas Hanscheid
    Institute of Molecular Medicine, Lisbon Medical College, Lisbon, Portugal
    Lancet Infect Dis 7:675-85. 2007
    ..Finally, haemozoin has a substantial effect on the immune system. Further research is needed to clarify these aspects, many of which are important in clinical practice...
  16. pmc A novel flow cytometric hemozoin detection assay for real-time sensitivity testing of Plasmodium falciparum
    Maria Rebelo
    Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Lisbon, Portugal
    PLoS ONE 8:e61606. 2013
    ..These results indicate that the reagent-free, real-time Hz detection assay could become a novel assay for the detection of drug effects on Plasmodium falciparum...
  17. ncbi request reprint Macrolide resistance in Streptococcus pneumoniae isolated from patients with community-acquired lower respiratory tract infections in Portugal: results of a 3-year (1999-2001) multicenter surveillance study
    J Melo-Cristino
    Laboratory of Microbiology, Institute of Molecular Medicine, Faculty of Medicine, University of Lisbon, 1649 028 Lisbon, Portugal
    Microb Drug Resist 9:73-80. 2003
    ..Because of the specific situation concerning macrolide resistance described in S. pneumoniae, careful use of macrolide antibiotics in therapy and cautious monitoring of macrolide resistance should be continued in Portugal...
  18. doi request reprint Use of flow cytometry (Sysmex) UF-100) to screen for positive urine cultures: in search for the ideal cut-off
    Sara Brilha
    Serviço de Patologia Clínica, Centro Hospitalar Lisboa Norte, Lisbon, Portugal
    Clin Chem Lab Med 48:289-92. 2010
    ..Several automated methods are available to screen urines for UTI, one being the flow cytometry-based Sysmex((R)) UF-100...
  19. doi request reprint Cerebral malaria and the hemolysis/methemoglobin/heme hypothesis: shedding new light on an old disease
    Ana Pamplona
    Unidade de Malaria, Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649 028 Lisboa, Portugal
    Int J Biochem Cell Biol 41:711-6. 2009
    ..In this review, we discuss the role of hemolysis, methemoglobin and free heme in CM, brought to light by our recent studies in mice as well as by other studies in humans...
  20. pmc In Vivo Hemozoin Kinetics after Clearance of Plasmodium berghei Infection in Mice
    Rosangela Frita
    Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649 028 Lisbon, Portugal
    Malar Res Treat 2012:373086. 2012
    ..Here, using several independent methods to assess the presence of Hz, we report the long-term in vivo kinetics of Hz in diverse organs in a murine model of malaria infection...
  21. ncbi request reprint Automated malaria diagnosis using pigment detection
    T Hanscheid
    Laboratorio de Bacteriologia, Piso 4, Patologia Clinica, Hospital de Santa Maria, P 1600, Lisboa, Portugal
    Parasitol Today 16:549-51. 2000
    ..Thomas Hänscheid, Emilia Valadas and Martin Grobusch here describe this novel technique for malaria diagnosis and discuss its potential applications...
  22. pmc Torins are potent antimalarials that block replenishment of Plasmodium liver stage parasitophorous vacuole membrane proteins
    Kirsten K Hanson
    Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649 028 Lisboa, Portugal
    Proc Natl Acad Sci U S A 110:E2838-47. 2013
    ....
  23. ncbi request reprint HIV Infection and non-tuberculous mycobacteria: how important in southern European countries?
    Emilia Valadas
    Clínica Universitária de Doenças Infecciosas, Faculdade de Medicina, Lisboa, Portugal
    Scand J Infect Dis 36:685-6. 2004
    ..The importance of these infections in some areas, the usefulness of prophylaxis or routine use of mycobacterial blood cultures, may have to be re-considered...
  24. ncbi request reprint Smear microscopy to diagnose tuberculosis early and prevent further transmission in a population with a high prevalence of HIV infection
    E Valadas
    Serviço de Doenças Infecciosas, Hospital de Santa Maria, Av Prof Egas Moniz, P 1649 028 Lisboa, Portugal
    Clin Microbiol Infect 9:1045-7. 2003
    ..Appropriate isolation facilities for TB are much needed, in order to prevent nosocomial TB spread...