Research Topics
| José BragaSummaryAffiliation: Faculdade de Medicina Country: Portugal Publications
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Detail Information
Publications
Intracellular macromolecular mobility measured by fluorescence recovery after photobleaching with confocal laser scanning microscopesJosé Braga
Institute of Molecular Medicine, Faculty of Medicine, University of Lisbon, 1649 028 Lisbon, Portugal
Mol Biol Cell 15:4749-60. 2004..This suggests that spatial constraints to diffusion of dextrans inside the nucleus are insensitive to temperature...- A reaction-diffusion model to study RNA motion by quantitative fluorescence recovery after photobleachingJosé Braga
Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal
Biophys J 92:2694-703. 2007..The results show that interpreting FRAP data in light of the new model reconciles the discrepant mRNA diffusion-rate values previously reported... - Inactivation of cleavage factor I components Rna14p and Rna15p induces sequestration of small nucleolar ribonucleoproteins at discrete sites in the nucleusTiago Carneiro
Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649 028 Lisboa, Portugal
Mol Biol Cell 19:1499-508. 2008..We propose that the foci detected after CFIA inactivation correspond to quality control centers in the nucleoplasm... 
Photobleaching microscopy reveals the dynamics of mRNA-binding proteins inside live cell nucleiJosé Braga
Institute of Molecular Medicine, Faculty of Medicine, University of Lisbon, 1649 028, Lisbon, Portugal
Prog Mol Subcell Biol 35:119-34. 2004
Frontiers in fluorescence microscopyJosé Rino
Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal
Int J Dev Biol 53:1569-79. 2009..It all changed abruptly during the last two decades when the rapid development of novel imaging techniques revolutionized the way scientists look at the structures of life alive...- A stochastic view of spliceosome assembly and recycling in the nucleusJosé Rino
Instituto de Medicina Molecular, Universidade de Lisboa, Lisbon, Portugal
PLoS Comput Biol 3:2019-31. 2007..Rather, the results suggest that splicing proteins are constantly diffusing throughout the entire nucleus and collide randomly and transiently with pre-mRNAs... - Dynamic transitions in RNA polymerase II density profiles during transcription terminationAna Rita Grosso
Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649 028 Lisboa, Portugal
Genome Res 22:1447-56. 2012..Taken together, our results provide novel insight to transcription termination, a fundamental process that remains one of the least understood stages of the transcription cycle... - Depletion of the yeast nuclear exosome subunit Rrp6 results in accumulation of polyadenylated RNAs in a discrete domain within the nucleolusTiago Carneiro
Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Av Prof Egas Moniz, 1649 028 Lisbon, Portugal
Mol Cell Biol 27:4157-65. 2007..The accumulation of polyadenylated snoRNAs in a discrete nucleolar domain may promote their recognition as substrates for the exosome... - In vivo aggregation properties of the nuclear poly(A)-binding protein PABPN1João Paulo Tavanez
Institute of Molecular Medicine, Faculty of Medicine, Av Prof Egas Moniz, 1649 028 Lisboa, Portugal
RNA 11:752-62. 2005..These findings have important implications for the interpretation of OPMD model systems based on exogenous expression of PABPN1... - Human topoisomerase IIalpha: targeting to subchromosomal sites of activity during interphase and mitosisMarta Agostinho
Institute of Histology, Faculty of Medicine, University of Lisbon, 1649 028 Lisbon, Portugal
Mol Biol Cell 15:2388-400. 2004..Together, our data implicate several previously unsuspected activities in the partitioning of the enzyme between sites of activity and putative depots...