N F Azevedo

Summary

Affiliation: Faculdade de Engenharia
Country: Portugal

Publications

  1. pmc Hybridization-based detection of Helicobacter pylori at human body temperature using advanced locked nucleic acid (LNA) probes
    Silvia Fontenete
    LEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M, Denmark ICBAS, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal
    PLoS ONE 8:e81230. 2013
  2. pmc BiofOmics: a Web platform for the systematic and standardized collection of high-throughput biofilm data
    Anália Lourenço
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal
    PLoS ONE 7:e39960. 2012
  3. pmc Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp
    Antonio Machado
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga 4710 057, Portugal
    BMC Microbiol 13:82. 2013
  4. pmc Proposal for a method to estimate nutrient shock effects in bacteria
    Nuno F Azevedo
    LEPAE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Portugal
    BMC Res Notes 5:422. 2012
  5. pmc PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori
    Laura Cerqueira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    BMC Microbiol 11:101. 2011
  6. pmc Interaction of Legionella pneumophila and Helicobacter pylori with bacterial species isolated from drinking water biofilms
    Maria S Gião
    School of Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK
    BMC Microbiol 11:57. 2011
  7. doi request reprint Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples
    N F Azevedo
    IBB, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal
    Antonie Van Leeuwenhoek 100:463-70. 2011
  8. ncbi request reprint Incorporation of natural uncultivable Legionella pneumophila into potable water biofilms provides a protective niche against chlorination stress
    M S Gião
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Braga, Portugal
    Biofouling 25:335-41. 2009
  9. ncbi request reprint Comparison between standard culture and peptide nucleic acid 16S rRNA hybridization quantification to study the influence of physico-chemical parameters on Legionella pneumophila survival in drinking water biofilms
    M S Gião
    Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Braga, Portugal
    Biofouling 25:343-51. 2009
  10. pmc Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Salmonella spp. in a broad spectrum of samples
    C Almeida
    Centro de Engenharia Biólogica, Universidade do Minho, 4710 057 Braga, Portugal
    Appl Environ Microbiol 76:4476-85. 2010

Collaborators

  • C W Keevil
  • Carina Almeida
  • S M Braganra
  • Laura Cerqueira
  • Ceu Figueiredo
  • Maria J Vieira
  • M S Gião
  • S A Wilks
  • Antonio Machado
  • Silvia Fontenete
  • Anália Lourenço
  • Maria S Gião
  • S Wilks
  • N Guimaraes
  • Marina Leite
  • Jesper Wengel
  • Nuno Guimarães
  • Mario Vaneechoutte
  • Nuno Filipe Azevedo
  • Lígia Rodrigues
  • Freddy Haesebrouck
  • Ana Henriques
  • Nuno Cerca
  • Débora Salgueiro
  • Nuno Veiga
  • Andreia Ferreira
  • Maria Olivia Pereira
  • Idalina Machado
  • Sandra A Wilks

Detail Information

Publications26

  1. pmc Hybridization-based detection of Helicobacter pylori at human body temperature using advanced locked nucleic acid (LNA) probes
    Silvia Fontenete
    LEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M, Denmark ICBAS, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal
    PLoS ONE 8:e81230. 2013
    ..pylori detection and location, which could be used in the future for potential in vivo applications either for this microorganism or for others...
  2. pmc BiofOmics: a Web platform for the systematic and standardized collection of high-throughput biofilm data
    Anália Lourenço
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal
    PLoS ONE 7:e39960. 2012
    ..However, biofilm data is widespread across ad hoc, non-standardized individual files and, thus, data interchange among researchers, or any attempt of cross-laboratory experimentation or analysis, is hardly possible or even attempted...
  3. pmc Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp
    Antonio Machado
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga 4710 057, Portugal
    BMC Microbiol 13:82. 2013
    ..Our goal was to develop and optimize a novel Peptide Nucleic Acid (PNA) Fluorescence in situ Hybridization assay (PNA FISH) for the detection of Lactobacillus spp. and G. vaginalis in mixed samples...
  4. pmc Proposal for a method to estimate nutrient shock effects in bacteria
    Nuno F Azevedo
    LEPAE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Portugal
    BMC Res Notes 5:422. 2012
    ..A nutrient shock is one of the mechanisms proposed to explain this phenomenon. In this study, a tentative method to assess nutrient shock effects was tested...
  5. pmc PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori
    Laura Cerqueira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    BMC Microbiol 11:101. 2011
    ..In this study, a new genotypic method to detect clarithromycin resistance in clinical samples, based on fluorescent in situ hybridization (FISH) using a set of peptide nucleic acid probes (PNA), is proposed...
  6. pmc Interaction of Legionella pneumophila and Helicobacter pylori with bacterial species isolated from drinking water biofilms
    Maria S Gião
    School of Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK
    BMC Microbiol 11:57. 2011
    ..This work aims to study the influence of five microorganisms isolated from drinking water biofilms on the survival and integration of both of these pathogens into biofilms...
  7. doi request reprint Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples
    N F Azevedo
    IBB, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal
    Antonie Van Leeuwenhoek 100:463-70. 2011
    ..The method was subsequently successfully adapted for the detection of S. epidermidis in mixed-species blood cultures both by microscopy and flow cytometry...
  8. ncbi request reprint Incorporation of natural uncultivable Legionella pneumophila into potable water biofilms provides a protective niche against chlorination stress
    M S Gião
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Braga, Portugal
    Biofouling 25:335-41. 2009
    ..pneumophila from re-entering an infectious state by maintaining residual disinfectant levels through the entire DWDS network so that the resuscitation of cells via contact with amoebae is prevented...
  9. ncbi request reprint Comparison between standard culture and peptide nucleic acid 16S rRNA hybridization quantification to study the influence of physico-chemical parameters on Legionella pneumophila survival in drinking water biofilms
    M S Gião
    Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Braga, Portugal
    Biofouling 25:343-51. 2009
    ..pneumophila. This raises public health concerns since culture methods are still considered to be the gold standard for assessing the presence of this opportunistic pathogen in water...
  10. pmc Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Salmonella spp. in a broad spectrum of samples
    C Almeida
    Centro de Engenharia Biólogica, Universidade do Minho, 4710 057 Braga, Portugal
    Appl Environ Microbiol 76:4476-85. 2010
    ....
  11. pmc Survival of gastric and enterohepatic Helicobacter spp. in water: implications for transmission
    N F Azevedo
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Appl Environ Microbiol 74:1805-11. 2008
    ..felis, H. canadensis, H. pullorum, and H. canis, in water under several conditions and concludes that the roles of water in transmission between hosts are likely to be similar for all these species, whether enterohepatic or not...
  12. ncbi request reprint Adhesion of water stressed Helicobacter pylori to abiotic surfaces
    N F Azevedo
    Centro de Engenharia Biólogica, Universidade do Minho, Braga, Portugal
    J Appl Microbiol 101:718-24. 2006
    ....
  13. doi request reprint Incorporation of natural uncultivable Legionella pneumophila into potable water biofilms provides a protective niche against chlorination stress
    M S Gião
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    Biofouling 25:345-51. 2009
    ..pneumophila from re-entering an infectious state by maintaining residual disinfectant levels through the entire DWDS network so that the resuscitation of cells via contact with amoebae is prevented...
  14. pmc Effect of chlorine on incorporation of Helicobacter pylori into drinking water biofilms
    M S Gião
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Appl Environ Microbiol 76:1669-73. 2010
    ..Coupled with data obtained using viability stains in pure culture, this result suggests that H. pylori can survive chlorination but remain undetectable by culture methods, which can be effectively replaced by PNA hybridization...
  15. pmc Detection of Escherichia coli O157 by peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) and comparison to a standard culture method
    C Almeida
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal
    Appl Environ Microbiol 79:6293-300. 2013
    ..22% (95% CI, 83.79 to 99.85%), and an accuracy of 98.33% (CI 95%, 83.41 to 99.91%). Results indicate that PNA-FISH performed as well as the traditional culture methods and can reduce the diagnosis time to 1 day. ..
  16. pmc Persistence of Helicobacter pylori in heterotrophic drinking-water biofilms
    M S Gião
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    Appl Environ Microbiol 74:5898-904. 2008
    ....
  17. ncbi request reprint Use of fluorescent in situ hybridisation for the visualisation of Helicobacter pylori in real drinking water biofilms
    S M Braganra
    Centro de Engenharia Biólogica, Universidade do Minho, 4700 057 Braga, Portugal
    Water Sci Technol 55:387-93. 2007
    ..The method requires, however, highly trained personnel for an accurate detection of the pathogen and will need simplification before being routinely used in standard water analysis laboratories...
  18. pmc Coccoid form of Helicobacter pylori as a morphological manifestation of cell adaptation to the environment
    N F Azevedo
    Centro de Engenharia Biólogica, Campus de Gualtar, Universidade do Minho, 4710 057 Braga, Portugal
    Appl Environ Microbiol 73:3423-7. 2007
    ..This strongly supports the view that transformation into the coccoid form is an active, biologically led process, switched on by the bacterium as a protection mechanism...
  19. ncbi request reprint A new model for the transmission of Helicobacter pylori: role of environmental reservoirs as gene pools to increase strain diversity
    N F Azevedo
    Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Braga, Portugal
    Crit Rev Microbiol 33:157-69. 2007
    ..pylori...
  20. pmc Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens
    N Guimaraes
    IBB Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    J Clin Microbiol 45:3089-94. 2007
    ..has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffin-embedded gastric biopsy specimens...
  21. doi request reprint Detection of Salmonella enterica serovar Enteritidis using real time PCR, immunocapture assay, PNA FISH and standard culture methods in different types of food samples
    C Almeida
    IBB, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
    Int J Food Microbiol 161:16-22. 2013
    ..In conclusion, the implementation of these molecular techniques, mainly the immunocapture and PNA-FISH methods, provides a reliable and less time-consuming alternative for the detection of Salmonella spp. in food samples...
  22. pmc Shear stress, temperature, and inoculation concentration influence the adhesion of water-stressed Helicobacter pylori to stainless steel 304 and polypropylene
    N F Azevedo
    Centro de Engenharia Biólogica, Universidade do Minho, 4710 057 Braga, Portugal
    Appl Environ Microbiol 72:2936-41. 2006
    ..It will also allow a more rational selection of locations to perform molecular or plate culture analysis for the detection of H. pylori in drinking water-associated biofilms...
  23. pmc Development and application of a novel peptide nucleic acid probe for the specific detection of Cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
    C Almeida
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, 4710 057 Braga, Portugal
    Appl Environ Microbiol 75:2925-30. 2009
    ..FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants...
  24. doi request reprint Validation of SYTO 9/propidium iodide uptake for rapid detection of viable but noncultivable Legionella pneumophila
    M S Gião
    Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Microb Ecol 58:56-62. 2009
    ..pneumophila cells in the environment...
  25. doi request reprint Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
    C Almeida
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057, Braga, Portugal
    Eur J Clin Microbiol Infect Dis 32:781-6. 2013
    ..Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h...
  26. pmc Nutrient shock and incubation atmosphere influence recovery of culturable Helicobacter pylori from water
    N F Azevedo
    Centro de Engenharia Biólogica, Universidade do Minho, 4700 057 Braga, Portugal
    Appl Environ Microbiol 70:490-3. 2004
    ..Besides highlighting and assessing the importance of several factors in the culturability of H. pylori, this paper demonstrates the potential ability to develop an optimized technique for recovery of this pathogen from water...