Diogo S Castro
Raposo A, Vasconcelos F, Drechsel D, Marie C, Johnston C, Dolle D, et al
. Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis. Cell Rep. 2015;10:1544-1556 pubmed publisher
..Our results reveal a function of Ascl1 in promoting chromatin accessibility during neurogenesis, linking the chromatin landscape at Ascl1 target regions with the temporal progression of its transcriptional program. ..
Castro D, Guillemot F. Old and new functions of proneural factors revealed by the genome-wide characterization of their transcriptional targets. Cell Cycle. 2011;10:4026-31 pubmed publisher
..Here we discuss our recent findings and review them in light of efforts from other laboratories to characterize the transcriptional programs downstream various proneural factors. ..
Monteiro C, Costa M, Reguenga C, Lima D, Castro D, Monteiro F. Paired related homeobox protein-like 1 (Prrxl1) controls its own expression by a transcriptional autorepression mechanism. FEBS Lett. 2014;588:3475-82 pubmed publisher
..Altogether, our results suggest that Prrxl1 uses distinct regulatory regions to repress its own expression in DRG and dorsal SC. ..
Vasconcelos F, Sessa A, Laranjeira C, Raposo A, Teixeira V, Hagey D, et al
. MyT1 Counteracts the Neural Progenitor Program to Promote Vertebrate Neurogenesis. Cell Rep. 2016;17:469-483 pubmed publisher
..These include regulators of the neural progenitor program, such as Hes1, Sox2, Id3, and Olig1. Thus, Ascl1 suppresses Notch signaling cell-autonomously via MyT1, coupling neuronal differentiation with repression of the progenitor fate. ..
Castro D, Martynoga B, Parras C, Ramesh V, Pacary E, Johnston C, et al
. A novel function of the proneural factor Ascl1 in progenitor proliferation identified by genome-wide characterization of its targets. Genes Dev. 2011;25:930-45 pubmed publisher
Soares M, Castro D. Chromatin Immunoprecipitation from Mouse Embryonic Tissue or Adherent Cells in Culture, Followed by Next-Generation Sequencing. Methods Mol Biol. 2018;1689:53-63 pubmed publisher
..This chapter describes a protocol for performing ChIP and ChIP-seq of transcription factors, starting either from mouse embryonic tissue or adherent cells in culture. ..