Sakorn Pornprasert

Summary

Publications

  1. ncbi request reprint Preventive Antiretroviral Therapy in Non-Thalassemia Carrier Infants Exposed to Mother-to-Child Transmission of HIV Decreases Cord and After Delivery Red Blood Production without Altering the Development of Hemoglobin
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, 110 Intawaroros Road, Chiang Mai, 50200, Thailand
    Curr HIV Res 12:227-31. 2014
  2. ncbi request reprint Criteria for detection of alpha-thalassemia-1 Thai type deletion in routine laboratory
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Clin Lab 59:1423-7. 2013
  3. ncbi request reprint Hematological characterization of compound heterozygous hemoglobin Hope/E patients with and without alpha-thalassemia-1 SEA type deletion
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Clin Lab 59:1183-5. 2013
  4. doi request reprint Detection of coinherited Hb H-Constant Spring/Paksé disease and Hb E by capillary electrophoresis and high performance liquid chromatography
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Hemoglobin 37:176-82. 2013
  5. ncbi request reprint HAART has no major impact on hematological and plasma bilirubin changes in HIV-infected patients with congenital G-6-PD deficiency
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Curr HIV Res 11:193-7. 2013
  6. ncbi request reprint Detection of beta-thalassemia/hemoglobin E disease in samples which initially were diagnosed as homozygous hemoglobin E
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Clin Lab 59:693-7. 2013
  7. doi request reprint Coinheritance of Hb S [β6(A3)Glu→Val, GAG>GTG] with β0-thalassemia codon 17 (A>T) in a Thai patient
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, 110 Intawaroros Road, Chiang Mai, Thailand
    Hemoglobin 36:265-9. 2012
  8. ncbi request reprint Unmasking Hb Paksé (codon 142, TAA>TAT, α2) and its combinations in patients also carrying Hb Constant Spring (codon 142, TAA>CAA, α2) in northern Thailand
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Hemoglobin 36:491-6. 2012
  9. ncbi request reprint Treatment of opportunistic infections prior to HAART initiation does not affect immune reconstitution in HIV-infected patients
    Sakorn Pornprasert
    Department of Medical Technology, Chiang Mai University, Chiang Mai, Thailand
    Curr HIV Res 10:592-6. 2012
  10. ncbi request reprint Rapid identification of heterozygous and homozygous hemoglobin constant spring by SYTO9 with a high resolution melting analysis
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Clin Lab 58:829-33. 2012

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Preventive Antiretroviral Therapy in Non-Thalassemia Carrier Infants Exposed to Mother-to-Child Transmission of HIV Decreases Cord and After Delivery Red Blood Production without Altering the Development of Hemoglobin
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, 110 Intawaroros Road, Chiang Mai, 50200, Thailand
    Curr HIV Res 12:227-31. 2014
    ..Therefore, ARV treatments for prevention of MTCT of HIV decreased RBC counts and hemoglobin but did not alter the development of Hb A, Hb F and Hb A2 of non-thalassemia carrier infants. ..
  2. ncbi request reprint Criteria for detection of alpha-thalassemia-1 Thai type deletion in routine laboratory
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Clin Lab 59:1423-7. 2013
    ....
  3. ncbi request reprint Hematological characterization of compound heterozygous hemoglobin Hope/E patients with and without alpha-thalassemia-1 SEA type deletion
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Clin Lab 59:1183-5. 2013
    ..There is no report of hematological data in patients with co-inheritance of compound heterozygous hemoglobin (Hb) Hope/E and alpha-thalassemia-1 Southeast Asian (SEA) type deletion...
  4. doi request reprint Detection of coinherited Hb H-Constant Spring/Paksé disease and Hb E by capillary electrophoresis and high performance liquid chromatography
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Hemoglobin 37:176-82. 2013
    ..0%) samples. Therefore, CE was proven to be superior to HPLC in the detection of Hb H-CS/Paksé-E disease, which will assist in diagnostic, counseling and prevention programs for these diseases...
  5. ncbi request reprint HAART has no major impact on hematological and plasma bilirubin changes in HIV-infected patients with congenital G-6-PD deficiency
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Curr HIV Res 11:193-7. 2013
    ..On the other hand, the effective use of HAART is associated with improvements in hemoglobin and MCH levels of these patients...
  6. ncbi request reprint Detection of beta-thalassemia/hemoglobin E disease in samples which initially were diagnosed as homozygous hemoglobin E
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Clin Lab 59:693-7. 2013
    ..The aim of this study is to observe the possibility of using Hb typing and hematological parameters to identify both disorders...
  7. doi request reprint Coinheritance of Hb S [β6(A3)Glu→Val, GAG>GTG] with β0-thalassemia codon 17 (A>T) in a Thai patient
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, 110 Intawaroros Road, Chiang Mai, Thailand
    Hemoglobin 36:265-9. 2012
    ..Therefore, to provide proper clinical management and genetic counseling of this rare case, DNA analysis should be performed in all cases when a peak at the S-window is detected by HPLC or CE...
  8. ncbi request reprint Unmasking Hb Paksé (codon 142, TAA>TAT, α2) and its combinations in patients also carrying Hb Constant Spring (codon 142, TAA>CAA, α2) in northern Thailand
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Hemoglobin 36:491-6. 2012
    ....
  9. ncbi request reprint Treatment of opportunistic infections prior to HAART initiation does not affect immune reconstitution in HIV-infected patients
    Sakorn Pornprasert
    Department of Medical Technology, Chiang Mai University, Chiang Mai, Thailand
    Curr HIV Res 10:592-6. 2012
    ..Our study indicates that treatment of OIs prior to HAART does not lead to impaired CD4 T-cells recovery and thymic outputs...
  10. ncbi request reprint Rapid identification of heterozygous and homozygous hemoglobin constant spring by SYTO9 with a high resolution melting analysis
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Clin Lab 58:829-33. 2012
    ..Gel-electrophoresis and ethidium bromide are not ideally suited to large scale analysis in clinical laboratories...
  11. doi request reprint Hb A2/E levels found in co-inheritance with the α-thalassemia-1 - -(SEA)/type deletion and either Hb E or β-thalassemia
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Hemoglobin 36:381-7. 2012
    ..0% of Hb E trait had Hb A(2)/E at higher levels. Accordingly, the Hb A(2)/E level at 21.54% is the best indicator for predicting co-inheritance of the α-thal-1 - -(SEA)/ deletion and Hb E trait...
  12. doi request reprint Quantification of hemoglobin Constant Spring in heterozygote and homozygote by a capillary electrophoresis method
    S Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Int J Lab Hematol 34:143-7. 2012
    ..Capillary electrophoresis (CE) is a high-resolution method for detection of hemoglobin Constant Spring (Hb CS)...
  13. pmc Detection of α-thalassemia-1 Southeast Asian and Thai type deletions and β-thalassemia 3.5-kb deletion by single-tube multiplex real-time PCR with SYBR Green1 and high-resolution melting analysis
    Sakorn Pornprasert
    Department of Medical Technology, Chiang Mai University, Chiang Mai, Thailand
    Korean J Lab Med 31:138-42. 2011
    ..Prevention and control of thalassemia requires simple, rapid, and accurate screening tests for carrier couples who are at risk of conceiving fetuses with severe thalassemia...
  14. ncbi request reprint Semi-nested Taqman real-time quantitative PCR for noninvasive prenatal diagnosis of Bart's hydrops fetalis
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    J Med Assoc Thai 95:6-9. 2012
    ..Non-invasive prenatal diagnosis based on detection of fetal cell-free DNA is limited when mother and father are both carriers for the same autosomal recessive mutation...
  15. ncbi request reprint Analysis of real-time PCR cycle threshold of alpha-thalassemia-1 Southeast Asian type deletion using fetal cell-free DNA in maternal plasma for noninvasive prenatal diagnosis of Bart's hydrops fetalis
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    J Med Assoc Thai 93:1243-8. 2010
    ..Noninvasive prenatal diagnosis based on detection of fetal cell-free DNA is hampered when mother and father are both carriers for the same autosomal recessive mutation...
  16. ncbi request reprint Hematological alterations and thymic function in newborns of HIV-infected mothers receiving antiretroviral drugs
    Rotjanee Wongnoi
    Department of Medical Technology, Faculty of Associated Medical Sciences, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai, Thailand
    Indian Pediatr 50:567-72. 2013
    ..To investigate the effects of antiretroviral (ARV) drugs on hematological parameters and thymic function in HIV-uninfected newborns of HIV-infected mothers...
  17. ncbi request reprint Effects of antiretroviral drugs for prevention of HIV-mother-to-child transmission on hematological parameters and hemoglobin synthesis in HIV-uninfected newborns with and without thalassemia carrier
    Rotjanee Wongnoi
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Curr HIV Res 11:187-92. 2013
    ..However, thalassemia and ARV drugs might have synergetic effect in inducing severe anemia...
  18. doi request reprint Evolution of hematological parameters in HIV-1-infected patients with and without thalassemia carriages during highly active antiretroviral therapy
    Sakorn Pornprasert
    Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    HIV Clin Trials 10:88-93. 2009
    ..To assess the effects of highly active antiretroviral therapy (HAART) on hematological parameters in HIV-1-infected patients with and without thalassemia carriages...
  19. doi request reprint Increased Hb A2 values in an HIV-1-infected patient receiving antiretroviral drugs: a pitfall for thalassemia antenatal diagnosis
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Hemoglobin 33:158-61. 2009
    ..However, if these tests are not available, the results of the red cell indices and osmotic fragility test should be considered as they may provide great value for beta-thal investigations...
  20. doi request reprint Comparison between capillary electrophoresis and high performance liquid chromatography for detection and quantification of Hb constant spring [Hb CS; α142, Term→Gln (TAA>CAA IN α2)]
    Jarurin Waneesorn
    Regional Medical Sciences Center, Chiang Mai, Thailand
    Hemoglobin 35:338-45. 2011
    ..Therefore, the CE method was superior to the HPLC method for detecting Hb CS. Furthermore, the level of Hb CS quantified by CE proved useful in screening heterozygotes and homozygotes with Hb CS as well as Hb H-CS disease...
  21. doi request reprint Diagnosis of thalassemia on dried blood spot samples by high performance liquid chromatography
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Hemoglobin 34:486-94. 2010
    ..Therefore, our results show that detection of thalassemia carriers using DBS is possible and is the alternative of choice in a low resource setting...
  22. doi request reprint Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the alpha-thalassemia-1 Southeast Asian type deletion: application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Hemoglobin 32:393-402. 2008
    ..Therefore, the two tubes real-time SYBR-PCR could represent a rapid, cost effective, high-throughput assay for screening of carriers and prenatal diagnosis of alpha-thalassemia-1 (alpha-thal-1) with the SEA type (--SEA) deletion...
  23. doi request reprint Comparison of real-time polymerase chain reaction SYBR Green1 with high resolution melting analysis and TaqMan MGB probes for detection of alpha-thalassemia-1 South-East Asian type on dried blood spots
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Eur J Haematol 81:405-7. 2008
  24. ncbi request reprint Unmasking tuberculosis-associated immune reconstitution inflammatory syndrome in HIV-1 infection after antiretroviral therapy
    Sakorn Pornprasert
    Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai
    Asian Pac J Allergy Immunol 28:206-9. 2010
    ..Therefore, the changing in these cytokine profiles may be an important marker of developing unmasking TB-IRIS...
  25. ncbi request reprint Comparison of capillary electrophoregram among heterozygous Hb Hope, Hb Hope/α-thalassemia-1 SEA type deletion and Hb Hope/β(0)-thalassemia
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Clin Chem Lab Med 50:1625-9. 2012
    ..However, there is no report of HbA(2) levels and capillary electrophoregrams for associations of heterozygote of Hb Hope with α-thalassemia nor β-thalassemia...
  26. doi request reprint SYTO9 and SYBR GREEN1 with a high-resolution melting analysis for prenatal diagnosis of β⁰-thalassemia/hemoglobin-E
    Sakorn Pornprasert
    Department of Medical Technology, Chiang Mai University, Thailand
    Eur J Haematol 85:424-9. 2010
    ..It is simple, flexible, non-destructive and has superb sensitivity and specificity. This approach might facilitate the laboratory diagnosis and genetic counseling for regions with a high prevalence of β⁰-thalassemia/Hb-E...
  27. ncbi request reprint Anemia in patients with coinherited thalassemia and glucose-6-phosphate dehydrogenase deficiency
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Hemoglobin 37:536-43. 2013
    ..02%). Mean levels of Hb, PCV, MCV and Hb A2/E were similar between the two groups. Thus, G-6-PD deficiency did not enhance red blood cell pathology or induce more anemic severity in thalassemia patients. ..
  28. ncbi request reprint Development of TaqMan real-time polymerase chain reaction for the detection and identification of Penicillium marneffei
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Mycoses 52:487-92. 2009
    ..Moreover, it can detect P. marneffei DNA in peripheral blood samples and blood-culture bottles. Therefore, the real-time PCR assay may represent a potential tool for early diagnosis of penicilliosis marneffei...
  29. ncbi request reprint Higher placental anti-inflammatory IL-10 cytokine expression in HIV-1 infected women receiving longer zidovudine prophylaxis associated with nevirapine
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Curr HIV Res 7:211-7. 2009
    ....
  30. ncbi request reprint SYTO9 and SYBR GREEN1 with high resolution melting analysis for molecular confirmatory testing of the common Southeast Asian beta(0)-thalassemia mutations
    Unthika Chamras
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand 50200
    Hemoglobin 33:539-45. 2009
    ..The SYTO9 and SYBR GREEN1 HRM analysis is simple and rapid. This approach will facilitate laboratory diagnosis and genetic counseling for regions with a high prevalence of beta-thal...
  31. ncbi request reprint Diagnosis of alpha-thalassemia-1 Southeast Asian type deletion and fetal gender by single-tube multiplex real-time PCR
    Sarinee Srithep
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Clin Lab 59:193-7. 2013
    ..This study is to develop a simple and cost-effective single tube multiplex real-time PCR for the diagnosis of alpha-thalassemia-1 SEA type deletion and detect fetal gender...
  32. ncbi request reprint Red Cell Indices and Formulas Used in Differentiation of β-Thalassemia Trait from Iron Deficiency in Thai School Children
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand and
    Hemoglobin 38:258-61. 2014
    ..4%), and the highest Youden's Index value (96.4%). Therefore, these formulas could be used in initial discrimination of the β-thal trait from iron deficiency in Thai school children. ..
  33. ncbi request reprint Blood Transfusion from a Hb E Trait Donor Can Affect β-Thalassemia Diagnosis
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand and
    Hemoglobin 38:295-8. 2014
    ..Moreover, CE has a high efficiency to prevent the misinterpretation of Hb analysis in patients who receive blood transfusions from a donor carrying Hb E. ..
  34. doi request reprint Detection of alpha-thalassemia-1 Southeast Asian type using real-time gap-PCR with SYBR Green1 and high resolution melting analysis
    Sakorn Pornprasert
    Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
    Eur J Haematol 80:510-4. 2008
    ..It is cost-effective and safe, does not require fluorescently labeled probe and hazardous chemicals. Moreover, it is accurate showing 100% concordance with conventional gap-PCR analysis...