Research Topics

Species

Steven A Porcelli

Summary

Publications

  1. Johndrow C, Goldberg M, Johnson A, Ng T, Kunnath Velayudhan S, Lauvau G, et al. Suppression of Th1 Priming by TLR2 Agonists during Cutaneous Immunization Is Mediated by Recruited CCR2+ Monocytes. J Immunol. 2018;201:3604-3616 pubmed publisher
    ..A more complete understanding of route-dependent TLR2 responses may be critical for informed design of novel subunit vaccines and for improvement of BCG and other vaccines based on live-attenuated organisms. ..
  2. Chennamadhavuni D, Saavedra Ávila N, Carreño L, Guberman Pfeffer M, Arora P, Yongqing T, et al. Dual Modifications of ?-Galactosylceramide Synergize to Promote Activation of Human Invariant Natural Killer T Cells and Stimulate Anti-tumor Immunity. Cell Chem Biol. 2018;25:571-584.e8 pubmed publisher
  3. Johnson A, Kennedy S, Ng T, Porcelli S. Identification of Novel Mycobacterial Targets for Murine CD4+ T-Cells by IFN? ELISPOT. Methods Mol Biol. 2018;1808:143-150 pubmed publisher
    ..Using this method, we identified novel mycobacterial antigens as vaccine candidates. ..
  4. Kennedy S, Johnson A, Bharrhan S, Lindestam Arlehamn C, Xu J, Garforth S, et al. Identification of Mycobacterial Ribosomal Proteins as Targets for CD4+ T Cells That Enhance Protective Immunity in Tuberculosis. Infect Immun. 2018;86: pubmed publisher
    ..tuberculosis. ..
  5. Kharkwal S, Arora P, Porcelli S. Glycolipid activators of invariant NKT cells as vaccine adjuvants. Immunogenetics. 2016;68:597-610 pubmed publisher
  6. Arora P, Kharkwal S, Ng T, Kunnath Velayudhan S, Saini N, Johndrow C, et al. Endocytic pH regulates cell surface localization of glycolipid antigen loaded CD1d complexes. Chem Phys Lipids. 2015;191:75-83 pubmed publisher
    ..These findings support a model in which low endosomal pH controls stability and lipid raft localization of CD1d-glycolipid complexes to regulate the outcome of iNKT cell mediated responses. ..
  7. Arora P, Kharkwal S, Ng T, Kunnath Velayudhan S, Saini N, Johndrow C, et al. "Endocytic pH regulates cell surface localization of glycolipid antigen loaded CD1d complexes". Chem Phys Lipids. 2016;194:49-57 pubmed publisher
    ..These findings support a model in which low endosomal pH controls stability and lipid raft localization of CD1d-glycolipid complexes to regulate the outcome of iNKT cell mediated responses. ..
  8. Arora P, Porcelli S. An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets. J Vis Exp. 2016;:e53824 pubmed publisher
    ..We have adapted this model to purify large numbers of functional dendritic cells for use in cell transfer experiments to compare in vivo proficiency of different DC subsets. ..
  9. Kunnath Velayudhan S, Porcelli S. Isolation of intact RNA from murine CD4+ T cells after intracellular cytokine staining and fluorescence-activated cell sorting. J Immunol Methods. 2018;456:77-80 pubmed publisher

More Information

Publications12

  1. Arora P, Baena A, Yu K, Saini N, Kharkwal S, Goldberg M, et al. A single subset of dendritic cells controls the cytokine bias of natural killer T cell responses to diverse glycolipid antigens. Immunity. 2014;40:105-16 pubmed publisher
    ..These findings show flexibility in the outcome of two-way communication between CD8?(+) dendritic cells and iNKT cells, providing a mechanism for biasing toward either proinflammatory or anti-inflammatory responses. ..
  2. Johnson A, Kennedy S, Lindestam Arlehamn C, Goldberg M, Saini N, Xu J, et al. Identification of Mycobacterial RplJ/L10 and RpsA/S1 Proteins as Novel Targets for CD4+ T Cells. Infect Immun. 2017;85: pubmed publisher
    ..tuberculosis and may help to inform the design of more effective vaccines against tuberculosis. ..
  3. Kunnath Velayudhan S, Goldberg M, Saini N, Johndrow C, Ng T, Johnson A, et al. Transcriptome Analysis of Mycobacteria-Specific CD4+ T Cells Identified by Activation-Induced Expression of CD154. J Immunol. 2017;199:2596-2606 pubmed publisher