Expression, purification, crystallization and preliminary crystallographic analysis of laminin-binding protein (Lmb) from Streptococcus agalactiaePreethi Ragunathan
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Chennai, India
Acta Crystallogr Sect F Struct Biol Cryst Commun 65:492-4. 2009
..The crystals diffracted to a resolution of 2.5 A and belonged to the monoclinic space group P2(1), with unit-cell parameters a = 56.63, b = 70.60, c = 75.37 A, beta = 96.77 degrees ...
Collagen adhesin-nanoparticle interaction impairs adhesin's ligand binding mechanismAribam Swarmistha Devi
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India
Biochim Biophys Acta 1820:819-28. 2012
..With the rapid rise in antibiotic resistance, designing alternative strategies to reduce/eliminate bacterial colonization is absolutely essential...
- Crystal structure of the first plant urease from jack bean: 83 years of journey from its first crystal to molecular structure
Anuradha Balasubramanian
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India
J Mol Biol 400:274-83. 2010
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- Structure of laminin-binding adhesin (Lmb) from Streptococcus agalactiae
Preethi Ragunathan
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India
Acta Crystallogr D Biol Crystallogr 65:1262-9. 2009
..Being ubiquitous in all serotypes of group B streptococcus (GBS), the structure of Lmb may direct the development of an efficient vaccine...
- The structure of putative N-acetyl glutamate kinase from Thermus thermophilus reveals an intermediate active site conformation of the enzyme
Ramya Sundaresan
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India
Biochem Biophys Res Commun 420:692-7. 2012
..The structural comparison of TtNAGK with EcNAGK revealed the structural basis of thermostability of TtNAGK and this information could be very useful to generate mutants of NAGK with increased overall stability...
- Expression, purification and structural analysis of a fibrinogen receptor FbsA from Streptococcus agalactiae
Preethi Ragunathan
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600025, India
Protein J 30:159-66. 2011
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- Crystal structure of type I 3-dehydroquinate dehydratase of Aquifex aeolicus suggests closing of active site flap is not essential for enzyme action
Aribam Swarmistha Devi
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India
Biochem Biophys Res Commun 432:350-4. 2013
..This provides new insights into the catalytic mechanism of type I 3-DHQases...
- Crystallization, characterization and preliminary X-ray crystallographic analysis of GK2848, a putative carbonic anhydrase of Geobacillus kaustophilus
Preethi Ragunathan
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India
Acta Crystallogr Sect F Struct Biol Cryst Commun 69:162-4. 2013
..The structure and functional analysis of GK2848 might provide valuable information on a novel class of carbonic anhydrases, as none of its homologous structures have been characterized...
- Cloning, expression, purification and ligand binding studies of novel fibrinogen-binding protein FbsB of Streptococcus agalactiae
Aribam Swarmistha Devi
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, India
Protein Expr Purif 74:148-55. 2010
..FbsB(C) indeed is a novel protein and understanding the mechanism of its interaction with Fg would be useful in developing strategies to fight against infections by Streptococcus...
- Expression, purification, crystallization and preliminary X-ray diffraction studies of the human keratin 4-binding domain of serine-rich repeat protein 1 from Streptococcus agalactiae
Ramya Sundaresan
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Chennai, India
Acta Crystallogr Sect F Struct Biol Cryst Commun 67:1582-5. 2011
..The crystals diffracted to 3.8 Å resolution using synchrotron radiation and belonged to space group P2(1), with unit-cell parameters a = 47.56, b = 59.48, c = 94.71 Å, β = 93.95°...
- Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the collagen-binding region of RspB from Erysipelothrix rhusiopathiae
Aribam Swarmistha Devi
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India
Acta Crystallogr Sect F Struct Biol Cryst Commun 66:156-9. 2010
..2 A using synchrotron radiation. The crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 46.19, b = 66.65, c = 101.72 A, beta = 94.11 degrees ...
- Purification, crystallization and preliminary X-ray analysis of urease from pigeon pea (Cajanus cajan)
Anuradha Balasubramanian
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India
Acta Crystallogr Sect F Struct Biol Cryst Commun 64:662-4. 2008
..The pigeon pea urease was crystallized and the resulting crystals diffracted to 2.5 A resolution. The crystals belong to the rhombohedral space group R32, with unit-cell parameters a = b = 176.29, c = 346.44 A...
- Purification, crystallization and preliminary X-ray analysis of urease from jack bean (Canavalia ensiformis)
Anuradha Balasubramanian
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, India
Acta Crystallogr Sect F Struct Biol Cryst Commun 65:949-51. 2009
..05 A resolution using synchrotron radiation. The crystals belonged to the hexagonal space group P6(3)22, with unit-cell parameters a = b = 138.57, c = 198.36 A...
- Crystal structure of ST2348, a CBS domain protein, from hyperthermophilic archaeon Sulfolobus tokodaii
Preethi Ragunathan
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India
Biochem Biophys Res Commun 375:124-8. 2008
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