Stephen J Polyak

Summary

Publications

  1. pmc Silibinin inhibits HIV-1 infection by reducing cellular activation and proliferation
    Janela McClure
    Department of Laboratory Medicine, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 7:e41832. 2012
  2. pmc Differential in vitro effects of intravenous versus oral formulations of silibinin on the HCV life cycle and inflammation
    Jessica Wagoner
    Department of Laboratory Medicine, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 6:e16464. 2011
  3. ncbi request reprint Silymarin for HCV infection
    Stephen J Polyak
    Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
    Antivir Ther 18:141-7. 2013
  4. pmc Identification of hepatoprotective flavonolignans from silymarin
    Stephen J Polyak
    Department of Laboratory Medicine, Microbiology and Global Health, University of Washington, Seattle, WA 98104, USA
    Proc Natl Acad Sci U S A 107:5995-9. 2010
  5. pmc Silymarin inhibits in vitro T-cell proliferation and cytokine production in hepatitis C virus infection
    Chihiro Morishima
    Division of Virology, Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, Washington 98104, USA
    Gastroenterology 138:671-81, 681.e1-2. 2010
  6. ncbi request reprint Inhibition of T-cell inflammatory cytokines, hepatocyte NF-kappaB signaling, and HCV infection by standardized Silymarin
    Stephen J Polyak
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98104 2499, USA
    Gastroenterology 132:1925-36. 2007
  7. ncbi request reprint Hepatitis C virus-specific immune responses and quasi-species variability at baseline are associated with nonresponse to antiviral therapy during advanced hepatitis C
    Chihiro Morishima
    Department of Laboratory Medicine and Pediatrics, University of Washington, Seattle, USA
    J Infect Dis 193:931-40. 2006
  8. ncbi request reprint Direct, Interferon-Independent Activation of the CXCL10 Promoter by NF-κB and Interferon Regulatory Factor 3 during Hepatitis C Virus Infection
    Jessica Brownell
    Department of Laboratory Medicine, University of Washington, Harborview Medical Center, Seattle, Washington, USA
    J Virol 88:1582-90. 2014
  9. pmc Arbidol: a broad-spectrum antiviral that inhibits acute and chronic HCV infection
    Yury S Boriskin
    Departments of Laboratory Medicine, Microbiology and Pathobiology, University of Washington, Seattle, USA
    Virol J 3:56. 2006
  10. pmc Independent, parallel pathways to CXCL10 induction in HCV-infected hepatocytes
    Jessica Brownell
    Department of Global Health, Pathobiology Program, University of Washington, Seattle, WA, United States
    J Hepatol 59:701-8. 2013

Collaborators

Detail Information

Publications25

  1. pmc Silibinin inhibits HIV-1 infection by reducing cellular activation and proliferation
    Janela McClure
    Department of Laboratory Medicine, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 7:e41832. 2012
    ..Silymarin-derived compounds provide cytoprotection by suppressing virus infection, immune activation, and inflammation, and as such may be relevant for both HIV mono-infected and HIV/HCV co-infected subjects...
  2. pmc Differential in vitro effects of intravenous versus oral formulations of silibinin on the HCV life cycle and inflammation
    Jessica Wagoner
    Department of Laboratory Medicine, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 6:e16464. 2011
    ..These data underscore the differences and similarities between the intravenous and oral formulations of silibinin, which could influence the clinical effects of this mixture on patients with chronic liver diseases...
  3. ncbi request reprint Silymarin for HCV infection
    Stephen J Polyak
    Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
    Antivir Ther 18:141-7. 2013
    ..This review will summarize the current state of knowledge on silymarin in the context of HCV infection. The information was collated from a recent workshop on silibinin in Germany...
  4. pmc Identification of hepatoprotective flavonolignans from silymarin
    Stephen J Polyak
    Department of Laboratory Medicine, Microbiology and Global Health, University of Washington, Seattle, WA 98104, USA
    Proc Natl Acad Sci U S A 107:5995-9. 2010
    ..The data suggest that silymarin- and silymarin-derived compounds may influence HCV disease course in some patients. Studies where standardized silymarin is dosed to identify specific clinical endpoints are urgently needed...
  5. pmc Silymarin inhibits in vitro T-cell proliferation and cytokine production in hepatitis C virus infection
    Chihiro Morishima
    Division of Virology, Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, Washington 98104, USA
    Gastroenterology 138:671-81, 681.e1-2. 2010
    ..The goal of this study was to determine whether silymarin has in vitro effects on immune function that might have implications for its potential effect on hepatitis C virus (HCV)-induced liver disease...
  6. ncbi request reprint Inhibition of T-cell inflammatory cytokines, hepatocyte NF-kappaB signaling, and HCV infection by standardized Silymarin
    Stephen J Polyak
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98104 2499, USA
    Gastroenterology 132:1925-36. 2007
    ..Milk thistle's clinical benefits for chronic hepatitis C are unsettled due to variability in standardization of the herbal product...
  7. ncbi request reprint Hepatitis C virus-specific immune responses and quasi-species variability at baseline are associated with nonresponse to antiviral therapy during advanced hepatitis C
    Chihiro Morishima
    Department of Laboratory Medicine and Pediatrics, University of Washington, Seattle, USA
    J Infect Dis 193:931-40. 2006
    ..Thus, in patients with advanced hepatic fibrosis due to HCV infection, pretreatment HCV-specific immune responses and increased QS variability appear to hinder viral clearance by pegylated IFN- alpha 2a and ribavirin combination therapy...
  8. ncbi request reprint Direct, Interferon-Independent Activation of the CXCL10 Promoter by NF-κB and Interferon Regulatory Factor 3 during Hepatitis C Virus Infection
    Jessica Brownell
    Department of Laboratory Medicine, University of Washington, Harborview Medical Center, Seattle, Washington, USA
    J Virol 88:1582-90. 2014
    ....
  9. pmc Arbidol: a broad-spectrum antiviral that inhibits acute and chronic HCV infection
    Yury S Boriskin
    Departments of Laboratory Medicine, Microbiology and Pathobiology, University of Washington, Seattle, USA
    Virol J 3:56. 2006
    ..ARB's affinity for membranes may inhibit several aspects of the HCV lifecycle that are membrane-dependent...
  10. pmc Independent, parallel pathways to CXCL10 induction in HCV-infected hepatocytes
    Jessica Brownell
    Department of Global Health, Pathobiology Program, University of Washington, Seattle, WA, United States
    J Hepatol 59:701-8. 2013
    ..We studied how hepatocyte sensing of early HCV infection via TLR3 (Toll-like receptor 3) and RIG-I (retinoic acid inducible gene I) led to expression of CXCL10...
  11. pmc Multiple effects of silymarin on the hepatitis C virus lifecycle
    Jessica Wagoner
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98104 2499, USA
    Hepatology 51:1912-21. 2010
    ..Silymarin inhibited microsomal triglyceride transfer protein activity, apolipoprotein B secretion, and infectious virion production into culture supernatants. Silymarin also blocked cell-to-cell spread of virus...
  12. pmc Regulation of CXCL-8 (interleukin-8) induction by double-stranded RNA signaling pathways during hepatitis C virus infection
    Jessica Wagoner
    Department of Laboratory Medicine, University of Washington, Virology 359690, 325 9th Avenue, Seattle, WA 98104 2499, USA
    J Virol 81:309-18. 2007
    ....
  13. pmc Relationships between hepatitis C virus replication and CXCL-8 production in vitro
    Bon Chang A Koo
    Virology Division, Department of Laboratory Medicine, 359690, 325 9th Avenue, Seattle, WA 98104 2499, USA
    J Virol 80:7885-93. 2006
    ....
  14. pmc Functional characterization of core genes from patients with acute hepatitis C virus infection
    Xi Tang
    Departments of Laboratory Medicine, University of Washington, Seattle, WA 98104, USA
    J Infect Dis 201:912-22. 2010
    ..The hepatitis C virus (HCV) core protein is implicated in diverse aspects of HCV-induced pathogenesis. There is a paucity of information on core in acute hepatitis C infection...
  15. pmc Hepatitis C virus induces oxidative stress, DNA damage and modulates the DNA repair enzyme NEIL1
    Sampa Pal
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195 7110 USA
    J Gastroenterol Hepatol 25:627-34. 2010
    ..In the current study we examined the induction of reactive oxygen species (ROS) resulting from HCV infection and evaluated its effect on the host DNA damage and repair machinery...
  16. doi request reprint Inhibition of HIV by Legalon-SIL is independent of its effect on cellular metabolism
    Janela McClure
    Department of Laboratory Medicine, University of Washington, Seattle, WA, United States
    Virology 449:96-103. 2014
    ..However, the metabolic effects are not involved in SIL's unique ability to block HIV entry. ..
  17. pmc Molecular pathways: hepatitis C virus, CXCL10, and the inflammatory road to liver cancer
    Jessica Brownell
    Pathobiology Program, Department of Global Health, University of Washington, Seattle, Washington 98104, USA
    Clin Cancer Res 19:1347-52. 2013
    ..We will also discuss potential host-directed therapies to slow or reverse HCV-induced inflammation that leads to fibrosis, cirrhosis, and HCCs...
  18. pmc Effect of ethanol on innate antiviral pathways and HCV replication in human liver cells
    Courtney R Plumlee
    Department of Laboratory Medicine, University of Washington, Seattle, USA
    Virol J 2:89. 2005
    ..To begin to address these issues, we characterized the interaction of acute ethanol on Jak-Stat and MAPK pathways in Huh7 cells, HCV replicon cells lines, and primary human hepatocytes...
  19. pmc Comparison of amplification enzymes for Hepatitis C Virus quasispecies analysis
    Stephen J Polyak
    Virology Division, Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
    Virol J 2:41. 2005
    ..QS diversity and complexity were quantified using heteroduplex mobility assays (HMA)...
  20. ncbi request reprint Hepatitis C virus--cell interactions and their role in pathogenesis
    Stephen J Polyak
    Department of Laboratory Medicine, University of Washington, Box 359690, 325 9th Avenue, Seattle, WA 98104 2499, USA
    Clin Liver Dis 7:67-88. 2003
    ....
  21. ncbi request reprint Effects of the hepatitis C virus core protein on innate cellular defense pathways
    Kristen Miller
    Virology Division, Department of Laboratory Medicine, University of Washington, Seattle, WA 98104 2499, USA
    J Interferon Cytokine Res 24:391-402. 2004
    ..Core stimulated the human iNOS promoter and induced iNOS protein. The data indicate that HCV core can modulate IRF, Jak-Stat, and iNOS pathways and suggest mechanisms by which core could affect HCV persistence and pathogenesis...
  22. pmc In vitro toxicity assessment of amphiphillic polymer-coated CdSe/ZnS quantum dots in two human liver cell models
    Wesley E Smith
    Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington 98195, United States
    ACS Nano 6:9475-84. 2012
    ..Thus, further in vivo studies are warranted to ensure that TOPO-PMAT-coated Qdots used in biomedical applications do not induce inflammatory responses as a consequence of hepatic uptake...
  23. pmc Unique features of hepatitis C virus capsid formation revealed by de novo cell-free assembly
    Kevin C Klein
    Department of Pathobiology, University of Washington, Seattle, Washington 98195, USA
    J Virol 78:9257-69. 2004
    ..By allowing reproducible and quantitative assessment of viral and cellular requirements for capsid formation, these cell-free systems make a mechanistic dissection of HCV capsid assembly possible...
  24. ncbi request reprint Stability of CXCL-8 and related AU-rich mRNAs in the context of hepatitis C virus replication in vitro
    Jamison Green
    Department of Laboratory Medicine, University of Washington, Seattle, 98104 2499, USA
    J Infect Dis 193:802-11. 2006
    ..CXCL-8 mRNA stability was, therefore, investigated in the context of HCV replication in 4 replicon cell lines, Huh7 and Huh7.5 cells, and primary human fetal hepatocytes...
  25. pmc Subversion of cell signaling pathways by hepatitis C virus nonstructural 5A protein via interaction with Grb2 and P85 phosphatidylinositol 3-kinase
    Yupeng He
    Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Virol 76:9207-17. 2002
    ....