Zbigniew Szewczuk

Summary

Country: Poland

Publications

  1. pmc New method of peptide cleavage based on Edman degradation
    Remigiusz Bąchor
    Faculty of Chemistry, University of Wrocław, ul F Joliot Curie 14, 50 383, Wrocław, Poland
    Mol Divers 17:605-11. 2013
  2. pmc The hydrogen-deuterium exchange at α-carbon atom in N,N,N-trialkylglycine residue: ESI-MS studies
    Magdalena Rudowska
    Faculty of Chemistry, University of Wrocław, Wrocław, Poland
    J Am Soc Mass Spectrom 23:1024-8. 2012
  3. pmc The competition of charge remote and charge directed fragmentation mechanisms in quaternary ammonium salt derivatized peptides--an isotopic exchange study
    Marzena Cydzik
    Faculty of Chemistry, University of Wrocław, F Joliot Curie 14, 50 383 Wrocław, Poland
    J Am Soc Mass Spectrom 22:2103-7. 2011
  4. pmc Gas-phase fragmentation of oligoproline peptide ions lacking easily mobilizable protons
    Magdalena Rudowska
    Faculty of Chemistry, University of Wrocław, Wrocław, Poland
    J Am Soc Mass Spectrom 24:846-56. 2013
  5. doi request reprint Dimeric analogs of immunosuppressive decapeptide fragment of ubiquitin
    Alicja Kluczyk
    Faculty of Chemistry, University of Wrocław, Wrocław, Poland
    J Pept Sci 18:456-65. 2012
  6. doi request reprint Detection of glycation sites in proteins by high-resolution mass spectrometry combined with isotopic labeling
    Piotr Stefanowicz
    Faculty of Chemistry, University of Wrocław, 50 137 Wrocław, Poland
    Anal Biochem 400:237-43. 2010
  7. doi request reprint The isotopic exchange of oxygen as a tool for detection of the glycation sites in proteins
    Monika Kijewska
    University of Wrocław, 50383 Wrocław, Poland
    Anal Biochem 419:81-7. 2011
  8. doi request reprint Testing isotopic labeling with [¹³C₆]glucose as a method of advanced glycation sites identification
    Martyna Kielmas
    University of Wrocław, 50 383 Wrocław, Poland
    Anal Biochem 431:57-65. 2012
  9. pmc Electron capture dissociation mass spectrometric analysis of lysine-phosphorylated peptides
    Karolina Kowalewska
    Faculty of Chemistry, University of Wrocław, 50 383 Wrocław, Poland
    Biosci Rep 30:433-43. 2010
  10. doi request reprint Methods of the site-selective solid phase synthesis of peptide-derived Amadori products
    Piotr Stefanowicz
    Faculty of Chemistry, University of Wrocław, F Joliot Curie 14, 50 383 Wrocław, Poland
    Amino Acids 38:881-9. 2010

Collaborators

  • Alicja Kluczyk
  • Michał Zimecki
  • Mariusz Jaremko
  • Jolanta Artym
  • Elzbieta Jankowska
  • Tomasz Ruman
  • Ignacy Z Siemion
  • Przemysław J Boratyński
  • Jacek Otlewski
  • Aneta Szymanska
  • Łukasz Jaremko
  • Joanna Cieśla
  • Piotr Stefanowicz
  • Monika Kijewska
  • Remigiusz Bąchor
  • Magdalena Rudowska
  • Marzena Cydzik
  • Martyna Kielmas
  • Katarzyna Kapczynska
  • Lukasz Jaremko
  • Paweł Pasikowski
  • Teresa Kowalik-Jankowska
  • Karolina Kowalewska
  • Marek Lisowski
  • Monika Biernat
  • Maciej Modzel
  • Dominika Bystranowska
  • Marta Pietruszka
  • Marian Kochman
  • Anna Łegowska
  • Andrzej Ozyhar
  • Małgorzata Koprowska-Ratajska
  • Tomasz M Kapłon
  • Marcin Paduch
  • Aleksandra Man-Kupisinska
  • Mateusz Waliczek
  • Adam Kuc
  • Bartosz Setner
  • Jolanta Lukasiewicz
  • Robert Wieczorek
  • Ewa Sitkiewicz
  • Dominika Wojewska
  • Piotr Dobryszycki
  • Maria Smużyńska
  • Tomasz Gozdziewicz
  • Elzbieta Bulak
  • Adam Sieradzan
  • Inga Petry-Podgórska
  • Tomasz Fraczyk
  • Franciszek Kasprzykowski
  • Anna Jaśkiewicz
  • Wojciech Rode
  • Adam Lesner
  • Krzysztof Rolka
  • Marek Cebrat
  • Hubert Bartosz-Bechowski
  • Igor Zhukov
  • Małgorzata Nocula-Ługowska
  • Michał Jakób
  • Grzegorz Rymarczyk
  • Zygmunt S Derewenda
  • Anna Staszewska
  • Andrzej Wilczynski

Detail Information

Publications37

  1. pmc New method of peptide cleavage based on Edman degradation
    Remigiusz Bąchor
    Faculty of Chemistry, University of Wrocław, ul F Joliot Curie 14, 50 383, Wrocław, Poland
    Mol Divers 17:605-11. 2013
    ..The applicability of the developed strategy in combinatorial chemistry was confirmed using a small training library of [Formula: see text]-chymotrypsin substrates. ..
  2. pmc The hydrogen-deuterium exchange at α-carbon atom in N,N,N-trialkylglycine residue: ESI-MS studies
    Magdalena Rudowska
    Faculty of Chemistry, University of Wrocław, Wrocław, Poland
    J Am Soc Mass Spectrom 23:1024-8. 2012
    ..Increased ionization efficiency, provided by the fixed positive charge on QAS group, as well as the deuterium labeling, enables the analysis of trace amounts of peptides...
  3. pmc The competition of charge remote and charge directed fragmentation mechanisms in quaternary ammonium salt derivatized peptides--an isotopic exchange study
    Marzena Cydzik
    Faculty of Chemistry, University of Wrocław, F Joliot Curie 14, 50 383 Wrocław, Poland
    J Am Soc Mass Spectrom 22:2103-7. 2011
    ..The isotopic labeling allows differentiation of fragments formed according to ChR and ChD mechanisms...
  4. pmc Gas-phase fragmentation of oligoproline peptide ions lacking easily mobilizable protons
    Magdalena Rudowska
    Faculty of Chemistry, University of Wrocław, Wrocław, Poland
    J Am Soc Mass Spectrom 24:846-56. 2013
    ..The study of the dissociation pattern highlights the unusual proline residue behavior during MS/MS experiments of peptides...
  5. doi request reprint Dimeric analogs of immunosuppressive decapeptide fragment of ubiquitin
    Alicja Kluczyk
    Faculty of Chemistry, University of Wrocław, Wrocław, Poland
    J Pept Sci 18:456-65. 2012
    ..Our results suggest that such orientation of peptide components may correspond to orientation of the hypothetic ubiquitin receptors responsible for the immunomodulatory activity...
  6. doi request reprint Detection of glycation sites in proteins by high-resolution mass spectrometry combined with isotopic labeling
    Piotr Stefanowicz
    Faculty of Chemistry, University of Wrocław, 50 137 Wrocław, Poland
    Anal Biochem 400:237-43. 2010
    ..The number of glycated peptides in the digest of HSA detected by our procedure was in agreement with the data recently reported in the literature...
  7. doi request reprint The isotopic exchange of oxygen as a tool for detection of the glycation sites in proteins
    Monika Kijewska
    University of Wrocław, 50383 Wrocław, Poland
    Anal Biochem 419:81-7. 2011
    ..The characteristic isotopic pattern of Amadori products treated with H(2)(18)O allows fast and convenient identification of this group of compounds, whereas nonglycated peptides are not susceptible to isotopic exchange...
  8. doi request reprint Testing isotopic labeling with [¹³C₆]glucose as a method of advanced glycation sites identification
    Martyna Kielmas
    University of Wrocław, 50 383 Wrocław, Poland
    Anal Biochem 431:57-65. 2012
    ..This isotopic labeling technique combined with LC-MS is a sensitive method for identification of advanced glycation end products even if their chemical structure is unknown...
  9. pmc Electron capture dissociation mass spectrometric analysis of lysine-phosphorylated peptides
    Karolina Kowalewska
    Faculty of Chemistry, University of Wrocław, 50 383 Wrocław, Poland
    Biosci Rep 30:433-43. 2010
    ..Moreover, the results indicate that the ECD-MS may be useful for analysis of regioselectivity of the N-phosphorylation reaction. Stabilities of the obtained lysine-phosphorylated peptides under various conditions were also tested...
  10. doi request reprint Methods of the site-selective solid phase synthesis of peptide-derived Amadori products
    Piotr Stefanowicz
    Faculty of Chemistry, University of Wrocław, F Joliot Curie 14, 50 383 Wrocław, Poland
    Amino Acids 38:881-9. 2010
    ..However, when the solvent was changed to trifluoroethanol, the glycated peptides preferred beta-turn conformation...
  11. doi request reprint A novel immunosuppressory peptide originating from the ubiquitin sequence
    Paweł Pasikowski
    Faculty of Chemistry, University of Wrocław, Wrocław, Poland
    Peptides 32:2418-27. 2011
    ..We also tested their immunosuppressory activity in in vitro experiments...
  12. doi request reprint A study on human serum albumin influence on glycation of fibrinogen
    Martyna Kielmas
    Faculty of Chemistry, University of Wrocław, 50 137 Wrocław, Poland
    Biochem Biophys Res Commun 439:78-83. 2013
    ..The obtained data indicate that the addition of HSA protects the fibrinogen from glycation. The level of glycation in presence of HSA is reduced by 30-60% and depends on the location of glycated residue in sequence of protein...
  13. ncbi request reprint Pressure as a denaturing agent in studies of single-point mutants of an amyloidogenic protein human cystatin c
    Elzbieta Jankowska
    Faculty of Chemistry, University of Gdansk, Gdansk, Poland
    Proteins 80:2417-25. 2012
    ..Our experiments also point out pressure denaturation as a valuable supplementary method in denaturation studies of mutated proteins...
  14. doi request reprint The efficient synthesis of isotopically labeled peptide-derived Amadori products and their characterization
    Katarzyna Kapczynska
    Faculty of Chemistry, University of Wrocław, F Joliot Curie 14, Wroclaw, Poland
    Amino Acids 40:923-32. 2011
    ..In this paper, we discussed various approaches to the synthesis of Amadori products labeled selectively with stable isotopes (2)H, (13)C and (18)O...
  15. ncbi request reprint Sensitive electrospray mass spectrometry analysis of one-bead-one-compound peptide libraries labeled by quaternary ammonium salts
    Remigiusz Bąchor
    University of Wrocław, ul F Joliot Curie 14, 50 383 Wrocław, Poland
    Mol Divers 16:613-8. 2012
    ..The developed strategy was applied to a small training library of α chymotrypsin substrates. The obtained results confirm the applicability of the proposed method in combinatorial chemistry...
  16. doi request reprint Electrospray ionization mass spectrometry as a method for studying the high-pressure denaturation of proteins
    Piotr Stefanowicz
    Faculty of Chemistry, University of Wroclaw, 50 383 Wroclaw, Poland
    Biosci Rep 30:91-9. 2010
    ....
  17. doi request reprint Electrospray ionization mass spectrometric analysis of complexes between peptide-derived Amadori products and borate ions
    Monika Kijewska
    Faculty of Chemistry, University of Wrocław, 50 383 Wrocław, Poland
    Rapid Commun Mass Spectrom 23:4038-46. 2009
    ..Moreover, the elimination of a whole hexose moiety was not detected in the examined peptides...
  18. doi request reprint The immunosuppressive activity and solution structures of ubiquitin fragments
    Lukasz Jaremko
    Faculty of Chemistry, University of Wrocław, Wrocław, Poland
    Biopolymers 91:423-31. 2009
    ..Although the peptide exhibited a well-defined conformation in methanol, its structure was distinct from the corresponding 50-59 fragment in the native ubiquitin molecule. (c) 2009 Wiley Periodicals, Inc. Biopolymers 91: 423-431, 2009...
  19. doi request reprint Inhibitory activity of double-sequence analogues of trypsin inhibitor SFTI-1 from sunflower seeds: an example of peptide splicing
    Anna Łegowska
    Faculty of Chemistry, University of Gdansk, Gdansk, Poland
    FEBS J 277:2351-9. 2010
    ..This process, analogous to that of the DNA and protein splicing, can be referred to as 'peptide splicing'...
  20. doi request reprint Hydrogen scrambling in non-covalent complexes of peptides
    Maciej Modzel
    Faculty of Chemistry, University of Wrocław, Joliot Curie 14, Wroclaw, Poland
    Rapid Commun Mass Spectrom 26:2739-44. 2012
    ..It is therefore important to find out how hydrogen scrambling occurs--this study concentrates on the possibility of scrambling between amino acid residues spatially close together, but not connected by covalent bonds...
  21. ncbi request reprint A mechanistic study on the fragmentation of peptide-derived Amadori products
    Piotr Stefanowicz
    Faculty of Chemistry, University of Wroclaw, F Joliot Curie 14, 50 383 Wroclaw, Poland
    J Mass Spectrom 44:1500-8. 2009
    ..On the basis of these observations we proposed a hypothetical mechanism of Amadori products' fragmentation...
  22. ncbi request reprint Complexation abilities of neuropeptide gamma toward copper(II) ions and products of metal-catalyzed oxidation
    Marta Pietruszka
    Faculty of Chemistry, University of Wrocław, Joliot Curie 14, 50 383 Wroclaw, Poland
    Inorg Chem 50:7489-99. 2011
    ..Oxidations of three histidine residues to 2-oxohistidines and fragmentations of Ac-NPG near the His (H(4), H(9),H(12)) residues support participation of the histidyl-imidazole nitrogen atoms in coordination of the metal ions...
  23. ncbi request reprint Threonine at position 6 is not essential for the immunosuppressive activity of HLA-DQ(beta164-172)-hexapeptide
    Piotr Stefanowicz
    Faculty of Chemistry, University of Wrocław, ul F Joliot Curie 14, 50 383 Wrocław, Poland
    Mol Immunol 41:911-7. 2004
    ..Interestingly, a simple analogue, pentapeptide amide H-Arg-Gly-Asp-Val-Tyr-NH2 possessed high immunosuppressive potency, comparable to that of cyclosporine...
  24. doi request reprint Microwave-assisted TFA cleavage of peptides from Merrifield resin
    Alicja Kluczyk
    Faculty of Chemistry, University of Wroclaw, F Joliot Curie 14, 50 383 Wroclaw, Poland
    J Pept Sci 16:31-9. 2010
    ....
  25. ncbi request reprint Hydrogen-deuterium exchange of α-carbon protons and fragmentation pathways in N-methylated glycine and alanine-containing peptides derivatized by quaternary ammonium salts
    Remigiusz Bąchor
    Faculty of Chemistry, University of Wrocław, F Joliot Curie 14, Wroclaw, Poland
    J Mass Spectrom 49:529-36. 2014
    ..Copyright © 2014 John Wiley & Sons, Ltd. ..
  26. doi request reprint Intramolecular cross-linking in the native JHBP molecule
    Dominika Bystranowska
    Department of Biochemistry, Wrocław University of Technology, Wrocław, Poland
    Arch Biochem Biophys 517:12-9. 2012
    ..MS analyses of peptides generated after JHBP proteolysis indicated, that the dityrosine bridge occurs between the Y128 and Y130 residues...
  27. doi request reprint Thiophosphorylation of free amino acids and enzyme protein by thiophosphoramidate ions
    Tomasz Ruman
    Rzeszow University of Technology, Faculty of Chemistry, Department of Biochemistry and Biotechnology, 6 Powstańców Warszawy Ave, 35 959 Rzeszow, Poland
    Bioorg Chem 38:74-80. 2010
    ..805 which is in perfect agreement with the simulated isotopic pattern distributions for monothiophosphate of TVQQQVHLNQDEYK where thiophosphate moiety is attached to histidine (His(26)) or lysine (Lys(33)) side-chain...
  28. doi request reprint Sequencing of peptide-derived Amadori products by the electron capture dissociation method
    Piotr Stefanowicz
    University of Wrocław, F Joliot Curie 14, 50 383 Wrocław, Poland
    J Mass Spectrom 44:1047-52. 2009
    ..The ECD experiments were conducted on a series of synthetic peptides and unseparated digests of glycated ubiquitin...
  29. ncbi request reprint Bivalent peptides as models for multimeric targets of PDZ domains
    Marcin Paduch
    Faculty of Biotechnology, Department of Protein Engineering, University of Wrocław, Tamka 2, 54 137 Wrocław, Poland
    Chembiochem 8:443-52. 2007
    ..This is consistent with the notion that multimerization of both receptors and PDZ-containing proteins might constitute an important regulatory mechanism...
  30. doi request reprint Derivatization of peptides as quaternary ammonium salts for sensitive detection by ESI-MS
    Marzena Cydzik
    Faculty of Chemistry, University of Wrocław, Wrocław, Poland
    J Pept Sci 17:445-53. 2011
    ..2.2]octane (DABCO) by iodoacetylated peptides is relatively easy and compatible with standard solid-phase peptide synthesis. This methodology offers a novel sensitive approach to analyze peptides and other compounds...
  31. pmc Selective detection of carbohydrates and their peptide conjugates by ESI-MS using synthetic quaternary ammonium salt derivatives of phenylboronic acids
    Monika Kijewska
    Faculty of Chemistry, University of Wroclaw, Wroclaw, Poland
    J Am Soc Mass Spectrom 25:966-76. 2014
    ..The obtained results suggest that derivatization of phenylboronic acid as QAS is a promising method for sensitive ESI-MS detection of carbohydrates and their conjugates formed by non-enzymatic glycation or glycosylation. ..
  32. doi request reprint The unusual hydrogen-deuterium exchange of α-carbon protons in N-substituted glycine-containing peptides
    Remigiusz Bąchor
    Faculty of Chemistry, University of Wrocław, F Joliot Curie 14, Wroclaw, Poland
    J Mass Spectrom 49:43-9. 2014
    ..Although the observed HDX of α-C protons is only successful in N-acylglycine when the amide possesses a certain degree of alkylation, it offers a new approach to the analysis of sarcosine-containing peptides such as cyclosporine...
  33. doi request reprint Solid phase synthesis of peptides containing novel amino acids, substituted 3-benzimidazolealanines
    Małgorzata Koprowska-Ratajska
    Faculty of Chemistry, University of Wrocław, F Joliot Curie 14, 50 383 Wrocław, Poland
    Amino Acids 36:309-15. 2009
    ..The heterocycle conjugates were obtained by reaction between aldehydes and peptides containing beta-(3,4-diaminophenyl)alanine residue, immobilized on a polymeric solid support...
  34. ncbi request reprint Amino-terminal dimerization of peptides on the solid support. Synthesis and biological activity of the immunosuppressive HLA-DR fragments linked by poly(ethylene glycol)s
    Monika Biernat
    Faculty of Chemistry, University of Wrocław, Wrocław, Poland
    Bioconjug Chem 17:1116-24. 2006
    ..MS/MS analysis of the obtained dimeric peptides is also presented...
  35. doi request reprint Starmaker exhibits properties of an intrinsically disordered protein
    Tomasz M Kapłon
    Department of Biochemistry, Faculty of Chemistry, Wrocław University of Technology, Wrocław, Poland
    Biomacromolecules 9:2118-25. 2008
    ..The functional significance of Stm having intrinsically disordered protein properties and its possible role in controlling the formation of otoliths is discussed...
  36. ncbi request reprint Coordination abilities of neurokinin A and its derivative and products of metal-catalyzed oxidation
    Teresa Kowalik-Jankowska
    Faculty of Chemistry, University of Wrocław, Joliot Curie 14, 50 383 Wrocław, Poland
    J Inorg Biochem 104:831-42. 2010
    ..Oxidation target for both studied peptides is the histidine residue coordinated to the metal ions. Both peptides contain Met and His residues and are very susceptible on the copper(II)-catalyzed oxidation...
  37. ncbi request reprint Immunosuppressory activity of ubiquitin fragments containing retro-RGD sequence
    Zbigniew Szewczuk
    Faculty of Chemistry, University of Wrocław, Wrocław, Poland
    Biopolymers 74:352-62. 2004
    ..The cyclization product selectively but strongly suppresses the cellular immune response. The results indicate that the 48-59 loop may serve as an important functional epitope in the ubiquitin molecule for intermolecular binding...