Research Topics
| D LechniakSummaryAffiliation: Polish Academy of Sciences Country: Poland Publications
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Detail Information
Publications
Sperm pre-incubation prior to insemination affects the sex ratio of bovine embryos produced in vitroD Lechniak
Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University of Poznan, Poznan, Poland
Reprod Domest Anim 38:224-7. 2003..It was concluded that prolonged sperm pre-incubation influences the rate of development and the sex ratio among hatched blastocysts...
IVM media, oocyte diameter and donor genotype at RYR1 locus in relation to the incidence of porcine diploid oocytes after maturation in vitroDorota Lechniak
Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University of Poznan, Wolynska 33, 60 637 Poznan, Poland
Theriogenology 64:202-12. 2005..4 microm. According to statistical analysis, diploidy was not related to the oocyte diameter. That trait, however, was influenced by the donor genotype at the RYR1 locus. The TT genotype was associated with a higher rate of diploidy...
Quantitative aspect of gene expression analysis in mammalian oocytes and embryosDorota Lechniak
Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University of Poznan, Poland
Reprod Biol 2:229-41. 2002..This paper deals with the description of the current methods commonly used for quantitative gene expression in mammalian oocytes and preimplantation embryos with a special attention paid to the real time PCR...
Gilts and sows produce similar rate of diploid oocytes in vitro whereas the incidence of aneuploidy differs significantlyDorota Lechniak
Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University of Poznan, Wolynska 33, 60 637, Poznan, Poland
Theriogenology 68:755-62. 2007..The significantly higher rate of aneuploidy among oocytes derived from gilt ovaries may contribute to the reduced developmental competence of gametes collected from nonmature female pigs...
The use of HOS test to evaluate membrane functionality of boar sperm capacitated in vitroD Lechniak
Department of Genetics and Animal Breeding, Agricultural University of Poznan, Poznan, Poland
Reprod Domest Anim 37:379-80. 2002..Thus the membrane integrity as well as functionality may be affected as shown by Harrison (1996). The aim of the present study was to analyse changes in sperm membrane integrity after in vitro capacitation by use of the HOS test...
Low incidence of chromosome aberrations in spermatozoa of fertile boarsMaciej Orsztynowicz
Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poland
Reprod Biol 11:224-35. 2011..Therefore, it can be assumed that the presence of unbalanced spermatozoa at the level observed in fertile males does not significantly affect their reproductive potential...
In vitro maturation and fertilisation of bovine oocytes in relation to GH gene polymorphism (Leu/Val)Dorota Lechniak
August Cieszkowski, Agricultural University of Poznan, Department of Genetics and Animal Breeding, Poland
Reprod Nutr Dev 42:275-80. 2002..It has to be mentioned, that due to the low frequency of the VV genotype (0.03), the results of the present study should be treated as preliminary and need further analysis...
Distribution of polymorphic forms at the porcine GH locus in a population of day-10 pig embryosDorota Lechniak
Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University of Poznan, ul Wołyńska 33, 60 637 Poznan, Poland
J Appl Genet 43:93-6. 2002..17, DD 0.83; and Hae II AA 0.33, AB 0.58; and BB 0.09. The Msp I CC genotype was not found among analysed embryos. To our knowledge, this is the first report on the genotype and allele distribution at the GH locus among early pig embryos...
The ploidy of in vitro matured bovine oocytes is related to the diameterDorota Lechniak
Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University of Poznan, Poland
Theriogenology 57:1303-8. 2002..01) than the haploid ones. We conclude that bigger oocytes underwent normal meiotic division, whereas their smaller counterparts tended to follow an abnormal path of maturation...
Timing of the first zygotic cleavage as a marker of developmental potential of mammalian embryosDorota Lechniak
Department of Genetics and Animal Breeding, Agricultural University of Poznan, Wolynska 33, 60 637 Poznan, Poland
Reprod Biol 8:23-42. 2008..In this paper we discuss some of the important aspects related to the timing of the first zygotic cleavage and its influence on the developmental competence of resulting embryos...
Growth hormone exerts no effect on the timing of the first zygotic cleavage in cattleE Pers-Kamczyc
Poznan University of Life Sciences, Department of Genetics and Animal Breeding, Wolynska 33, 60 637 Poznan, Poland
Theriogenology 74:581-95. 2010..This is particularly relevant, since those blastocysts were very few and usually characterized by an impaired morphology (e.g., presence of fragmented blastomeres, smaller size)...
Cytogenetic analysis of horse oocytes matured in vitro for different periods of timeJ Sosnowski
Department of Genetics and Animal Breeding, Agricultural University of Poznan, Poland
Reprod Nutr Dev 37:63-8. 1997..2% of oocytes after 24 h, 70.4% of oocytes after 30 h, 75.0% of oocytes after 36 h and 74.4% of oocytes after 42 h of in vitro maturation...
Maturation medium supplements affect transcript level of apoptosis and cell survival related genes in bovine blastocysts produced in vitroE Warzych
Department of Animal Genetics and Breeding, Agricultural University of Poznan, Wolynska, Poznan, Poland
Mol Reprod Dev 74:280-9. 2007..Thus, under described conditions, fafBSA may be the optimal supplement to IVM medium due to higher transcript level of growth factor coding genes accompanied by a lower transcript level of Hsp70...
Supplements to in vitro maturation media affect the production of bovine blastocysts and their apoptotic index but not the proportions of matured and apoptotic oocytesE Warzych
Department of Animal Genetics and Breeding, Agricultural University of Poznan, Wolynska 33, 60 637 Poznan, Poland
Anim Reprod Sci 97:334-43. 2007....
Growth hormone gene expression in oocytes and zygotes produced by cows heterozygous (Leu127Val) at GH locusEwelina Warzych
Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University of Poznan, Poznan, Poland
Reprod Biol 4:259-69. 2004..These results indicate a possible influence of sub-optimal in vitro culture conditions on the distribution of genetic variants of GH gene transcripts in bovine oocytes and zygotes...
No single way to explain cytoplasmic maturation of oocytes from prepubertal and cyclic giltsP Pawlak
Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, Poland
Theriogenology 78:2020-30. 2012....
Disturbances of nuclear maturation in BCB positive oocytes collected from peri-pubertal giltsP Pawlak
Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Wolynska 33, 60 637 Poznan, Poland
Theriogenology 75:832-40. 2011..Significantly more diploid oocytes was derived from prepubertal ovaries, whereas the frequency of aneuploidy was similar in NCL and CL gilts...
Gene expression and protein distribution of leptin and its receptor in bovine oocytes and preattachment embryos produced in vitroZ E Madeja
1Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Wolynska 33, 60 637 Poznan, Poland
Animal 3:568-78. 2009..These results support previous findings on polar distribution of proteins within mammalian oocytes and embryos, as well as suggests leptin's potential role during early mammalian development and implantation...
Low incidence of diploidy among equine oocytes matured in vitroD Lechniak
August Cieszkowski Agricultural University of Poznan, Department of Genetics and Animal Breeding, Poznan, Poland
Equine Vet J 34:738-40. 2002
Early cleaved bovine embryos show reduced incidence of chromosomal aberrations and higher developmental potential on day 4.5 post-inseminationE Pers-Kamczyc
Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, Poland
Reprod Domest Anim 47:899-906. 2012..The present study also confirmed the usefulness of the FZC as a marker of embryo quality by demonstrating a significantly lower incidence of aberrations in early embryos...
A new SNP in the 3'-UTR of the hsp 70-1 gene in Bos taurus and Bos indicusT Adamowicz
Department of Genetics and Animal Breeding, Agricultural University of Poznan, Wolynska 33, Poznan, 60-637 Poland
Biochem Genet 43:623-7. 2005
The quality of porcine oocytes is affected by sexual maturity of the donor giltPiotr Pawlak
Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, Poland
Reprod Biol 11:1-18. 2011..In conclusion, high incidence of apoptosis and a big variation in the diameter of more competent BCB+ oocytes make the BCB test a less effective selection tool than previously reported...
