Research Topics
Genomes and GenesSpecies | Sławomir MilewskiSummaryCountry: Poland Publications
| Collaborators
|
Detail Information
Publications
Enzymes of UDP-GlcNAc biosynthesis in yeastSławomir Milewski
Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, Gdansk, Poland
Yeast 23:1-14. 2006....
Structural analogues of reactive intermediates as inhibitors of glucosamine-6-phosphate synthase and phosphoglucose isomeraseSławomir Milewski
Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, 11 12 Narutowicza St, 80 952 Gdansk, Poland
Arch Biochem Biophys 450:39-49. 2006..The Arg272, Lys210, and Gly271 peptide bond nitrogen system, present in the corresponding space of rabbit PGI, creates an area of positive MEP, stabilizing cis-enolate intermediate and attracting its structural mimics, such as 5PA...
Rational design of N-alkyl derivatives of 2-amino-2-deoxy-d-glucitol-6P as antifungal agentsAnna Melcer
Department of Chemistry, University of Gdansk, 18 Sobieskiego Street, 80 952 Gdansk, Poland
Bioorg Med Chem Lett 17:6602-6. 2007....
Functional domains and interdomain communication in Candida albicans glucosamine-6-phosphate synthaseJarosław Olchowy
Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, 11 12 Narutowicza St, 80 952 Gdansk, Poland
Biochem J 404:121-30. 2007..The W97G and V711F mutations resulted in an almost complete elimination of the GlcN-6-P-synthetic activity, with the retention of the amidohydrolase and sugar phosphate-isomerizing activities...
Construction, purification, and functional characterization of His-tagged Candida albicans glucosamine-6-phosphate synthase expressed in Escherichia coliJarosław Olchowy
Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, 11 12 Narutowicza St, 80 952 Gdansk, Poland
Protein Expr Purif 46:309-15. 2006..5% of the synthetic activity...
Chemical reactivity and antimicrobial activity of N-substituted maleimidesNatalia Salewska
Department of Organic Chemistry, Gdansk University of Technology, Organic Chemistry, Gdansk, Poland
J Enzyme Inhib Med Chem 27:117-24. 2012..The membrane enzyme, β(1,3)glucan synthase has been proposed as a putative primary target of N-ethylmaleimide and some of its analogues in Candida albicans cells...
Long range molecular dynamics study of regulation of eukaryotic glucosamine-6-phosphate synthase activity by UDP-GlcNAcAleksandra Miszkiel
Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, ul Narutowicza 11 12, 80 233 Gdansk, Poland
J Mol Model 17:3103-15. 2011..The obtained results constitute an important step toward understanding of mechanism of GlcN-6-P synthase inhibition by UDP-GlcNAc molecule...
Glucosamine-6-phosphate synthase, a novel target for antifungal agents. Molecular modelling studies in drug designMarek Wojciechowski
Gdansk University of Technology, Department of Pharmaceutical Technology and Biochemistry, Gdansk, Poland
Acta Biochim Pol 52:647-53. 2005..The novel ligand was designed and synthesised. Biological experiments confirmed our predictions. The new compound named ADMP is a much better inhibitor of glucosamine-6-phosphate synthase than ADGP...
Voriconazole and multidrug resistance in Candida albicansRoland Wakiec
Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, Gdansk, Poland
Mycoses 50:109-15. 2007..Voriconazole effectively competed with rhodamine 6G for the active efflux mediated by CaCdr1p and CaCdr2p...
Inactivation of glucosamine-6-phosphate synthase by N3-oxoacyl derivatives of L-2,3-diaminopropanoic acidRobert Jedrzejczak
Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, Gdansk, Poland
Chembiochem 13:85-96. 2012..Molecular mechanisms of the reactions studied are proposed...
Disruption of homocitrate synthase genes in Candida albicans affects growth but not virulenceKrzysztof Kur
Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, 11 12 Narutowicza St, 80 233, Gdansk, Poland
Mycopathologia 170:397-402. 2010..These findings strongly question a possibility of exploitation of homocitrate synthase and possibly also other enzymes of the lysine biosynthetic pathway as targets in chemotherapy of disseminated fungal infections...
Homoisocitrate dehydrogenase from Candida albicans: properties, inhibition, and targeting by an antifungal pro-drugIwona Gabriel
Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, Gdansk, Poland
FEMS Yeast Res 13:143-55. 2013..TMCBMA is the first reported antifungal for which an enzyme of the AAP was identified as a molecular target...
Antifungal activity of homoaconitate and homoisocitrate analogsMaria J Milewska
Department of Organic Chemistry, Gdansk University of Technology, 11 12 Narutowicza Str, 80 233 Gdansk, Poland
Molecules 17:14022-36. 2012..The highest antifungal activity was found for trimethyl trans-homoaconitate, which inhibited growth of some human pathogenic yeasts with minimal inhibitory concentration (MIC) values of 16-32 mg/mL...
Antifungal action of the oxathiolone-fused chalcone derivativeIzabela Łacka
Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, Gdansk, Poland
Mycoses 54:e407-14. 2011..The chalcone derivative may be a useful lead compound for the development of novel antifungal agents...
Engineering Candida albicans glucosamine-6-phosphate synthase for efficient enzyme purificationJustyna Czarnecka
Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, Poland
J Mol Recognit 25:564-70. 2012..The construct containing the 655-660 hexaHis insert was found to be a homodimeric protein, which is the first reported example of such quaternary structure of glucosamine-6-phosphate synthase of eukaryotic origin...
Glucosamine-6-phosphate synthase--the multi-facets enzymeSławomir Milewski
Department of Pharmaceutical Technology and Biochemistry, Technical University of Gdansk, ul Narutowicza 11 12, 80 952 Gdansk, Poland
Biochim Biophys Acta 1597:173-92. 2002..Most of these issues, especially their molecular aspects, have been extensively studied in recent years. This article provides a comprehensive overview of the present knowledge on this multi-facets enzyme...
Biofilm production and presence of ica and bap genes in Staphylococcus aureus strains isolated from cows with mastitis in the eastern PolandPiotr Szweda
Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poland
Pol J Microbiol 61:65-9. 2012..6 %) were classified as biofilm producers depending on the value of absorbance in the microtiter plate test. All of the isolates tested were found to possess both icaA and icaD genes, while the bap gene was absent in all strains...
