Grzegorz Dubin

Summary

Affiliation: Jagiellonian University
Country: Poland

Publications

  1. ncbi request reprint Bacterial proteases in disease - role in intracellular survival, evasion of coagulation/ fibrinolysis innate defenses, toxicoses and viral infections
    Grzegorz Dubin
    Departments of Microbiology, Jagiellonian University, Krakow, Poland
    Curr Pharm Des 19:1090-113. 2013
  2. doi request reprint Structural and functional characterization of SplA, an exclusively specific protease of Staphylococcus aureus
    Justyna Stec-Niemczyk
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biochem J 419:555-64. 2009
  3. pmc Biochemical and structural characterization of SplD protease from Staphylococcus aureus
    Michal Zdzalik
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    PLoS ONE 8:e76812. 2013
  4. doi request reprint Substrate specificity of Staphylococcus aureus cysteine proteases--Staphopains A, B and C
    Magdalena Kalińska
    Department of Microbiology, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    Biochimie 94:318-27. 2012
  5. doi request reprint α1-Antichymotrypsin inactivates staphylococcal cysteine protease in cross-class inhibition
    Benedykt Wladyka
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    Biochimie 93:948-53. 2011
  6. pmc Isolation, biochemical characterization, and cloning of a bacteriocin from the poultry-associated Staphylococcus aureus strain CH-91
    Benedykt Wladyka
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    Appl Microbiol Biotechnol 97:7229-39. 2013
  7. ncbi request reprint The staphostatin family of cysteine protease inhibitors in the genus Staphylococcus as an example of parallel evolution of protease and inhibitor specificity
    Grzegorz Dubin
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biol Chem 388:227-35. 2007
  8. pmc A novel matrix metalloprotease-like enzyme (karilysin) of the periodontal pathogen Tannerella forsythia ATCC 43037
    Abdulkarim Y Karim
    Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Krakow 30 387, Poland
    Biol Chem 391:105-17. 2010
  9. doi request reprint A regulatory role for Staphylococcus aureus toxin-antitoxin system PemIKSa
    Michal Bukowski
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Nat Commun 4:2012. 2013
  10. pmc Exfoliative toxins of Staphylococcus aureus
    Michal Bukowski
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Toxins (Basel) 2:1148-65. 2010

Collaborators

Detail Information

Publications21

  1. ncbi request reprint Bacterial proteases in disease - role in intracellular survival, evasion of coagulation/ fibrinolysis innate defenses, toxicoses and viral infections
    Grzegorz Dubin
    Departments of Microbiology, Jagiellonian University, Krakow, Poland
    Curr Pharm Des 19:1090-113. 2013
    ..As such, the potential benefits of pharmacological interference in relevant pathways for the struggle against bacterial pathogens are also discussed...
  2. doi request reprint Structural and functional characterization of SplA, an exclusively specific protease of Staphylococcus aureus
    Justyna Stec-Niemczyk
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biochem J 419:555-64. 2009
    ..Of interest, even though precise N-terminal trimming is a common regulatory mechanism among S1 family enzymes, the crystal structure of SplA reveals novel significantly different mechanistic details...
  3. pmc Biochemical and structural characterization of SplD protease from Staphylococcus aureus
    Michal Zdzalik
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    PLoS ONE 8:e76812. 2013
    ..56 Å and 2.1 Å. Molecular modeling and mutagenesis studies allowed us to define a consensus model of substrate binding, and illustrated the molecular mechanism of protease specificity. ..
  4. doi request reprint Substrate specificity of Staphylococcus aureus cysteine proteases--Staphopains A, B and C
    Magdalena Kalińska
    Department of Microbiology, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    Biochimie 94:318-27. 2012
    ..However, we show that at the same time the overall specificity of staphopains is relaxed, insofar as multiple substrates that diverge from the sequences described above are also efficiently hydrolyzed...
  5. doi request reprint α1-Antichymotrypsin inactivates staphylococcal cysteine protease in cross-class inhibition
    Benedykt Wladyka
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    Biochimie 93:948-53. 2011
    ..This evidence is consistent with the suicide-substrate-like mechanism of inhibition...
  6. pmc Isolation, biochemical characterization, and cloning of a bacteriocin from the poultry-associated Staphylococcus aureus strain CH-91
    Benedykt Wladyka
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    Appl Microbiol Biotechnol 97:7229-39. 2013
    ..aureus CH-91, dominated in S. aureus strains of poultry origin, although strains with variant II were also identified in this group. S. aureus strains of human origin were characterized exclusively by variant II. ..
  7. ncbi request reprint The staphostatin family of cysteine protease inhibitors in the genus Staphylococcus as an example of parallel evolution of protease and inhibitor specificity
    Grzegorz Dubin
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biol Chem 388:227-35. 2007
    ..The tight coevolution is likely the result of the known deleterious effects of uncontrolled staphopain action...
  8. pmc A novel matrix metalloprotease-like enzyme (karilysin) of the periodontal pathogen Tannerella forsythia ATCC 43037
    Abdulkarim Y Karim
    Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Krakow 30 387, Poland
    Biol Chem 391:105-17. 2010
    ..This specificity and the ability to degrade elastin, fibrinogen and fibronectin may contribute to the pathogenicity of periodontitis...
  9. doi request reprint A regulatory role for Staphylococcus aureus toxin-antitoxin system PemIKSa
    Michal Bukowski
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Nat Commun 4:2012. 2013
    ..Elucidation of this mechanism is particularly interesting because reversible activation is a prerequisite for the proposed general regulatory role of toxin-antitoxin systems...
  10. pmc Exfoliative toxins of Staphylococcus aureus
    Michal Bukowski
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Toxins (Basel) 2:1148-65. 2010
    ..The fascinating road leading to the discovery of ETs as the agents responsible for SSSS and the characterization of the molecular mechanism of their action, including recent advances in the field, are reviewed in this article...
  11. ncbi request reprint Characterisation of a highly specific, endogenous inhibitor of cysteine protease from Staphylococcus epidermidis, a new member of the staphostatin family
    Grzegorz Dubin
    Faculty of Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Biol Chem 385:543-6. 2004
    ..The difference in the susceptibility of individual inhibitors to proteolytic cleavage at the reactive site suggests subtle variations in the mechanism of interaction with cysteine proteases...
  12. doi request reprint Activation mechanism of thiol protease precursor from broiler chicken specific Staphylococcus aureus strain CH-91
    Benedykt Wladyka
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    Vet Microbiol 147:195-9. 2011
    ..Both activation pathways allow efficient production of mature extracellular thiol protease, a putative virulence factor specific for avian strains of S. aureus...
  13. doi request reprint Enzymatic activity of the Staphylococcus aureus SplB serine protease is induced by substrates containing the sequence Trp-Glu-Leu-Gln
    Grzegorz Dubin
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    J Mol Biol 379:343-56. 2008
    ..Finally, to guide future development, we attempt to point at likely physiological substrates and thus the role of SplB in staphylococcal physiology...
  14. doi request reprint Prevalence of genes encoding extracellular proteases in Staphylococcus aureus - important targets triggering immune response in vivo
    Michal Zdzalik
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    FEMS Immunol Med Microbiol 66:220-9. 2012
    ..Overall, the results presented in this study support the hypothesis that the secretory proteases as a group may contribute to the virulence of S. aureus...
  15. ncbi request reprint Interaction of regulators Mdm2 and Mdmx with transcription factors p53, p63 and p73
    Michal Zdzalik
    Department of Microbiology, Faculty of Biochemistry, Jagiellonian University, Krakow, Poland
    Cell Cycle 9:4584-91. 2010
    ..Finally, the contribution of these findings to our understanding of the physiological role of these interactions is discussed...
  16. doi request reprint The virulence of Staphylococcus aureus correlates with strain genotype in a chicken embryo model but not a nematode model
    Klaudia Polakowska
    Department of Microbiology, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    Microbes Infect 14:1352-62. 2012
    ..Our findings indicate the potential usefulness of embryo model for future identification of host-specific adaptations and virulence factors in S. aureus...
  17. doi request reprint Mdm2 and MdmX inhibitors for the treatment of cancer: a patent review (2011-present)
    Krzysztof Zak
    Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology, Krakow, Poland
    Expert Opin Ther Pat 23:425-48. 2013
    ..Pharmacological disruption of the Mdm2/X-p53 interaction to restore p53 activity is an attractive concept, aiming at a targeted and non-toxic cancer treatment...
  18. ncbi request reprint Evaluation of P1' substrate specificity of staphylococcal SplB protease
    Katarzyna Pustelny
    Department of Analytical Biochemistry and Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Acta Biochim Pol 61:149-52. 2014
    ..Kinetic data on hydrolysis of a panel of substrates substituted at the said position is provided. ..
  19. ncbi request reprint Staphylococcus aureus-derived staphopain B, a potent cysteine protease activator of plasma chemerin
    Paulina Kulig
    Department of Immunology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Ulica Gronostajowa 7, 30 387 Krakow, Poland
    J Immunol 178:3713-20. 2007
    ..SspB may help direct the recruitment of specialized host cells, including immunoregulatory pDC and/or macrophages, contributing to the ability of S. aureus to elicit and maintain a chronic inflammatory state...
  20. ncbi request reprint New generation of peptide antibiotics
    Adam Dubin
    Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
    Acta Biochim Pol 52:633-8. 2005
    ..The article summarizes recent advances in both fields of study and presents perspectives for further development and possible applications...
  21. ncbi request reprint A novel class of cysteine protease inhibitors: solution structure of staphostatin A from Staphylococcus aureus
    Grzegorz Dubin
    Faculty of Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Biochemistry 42:13449-56. 2003
    ....