Andrzej Dziembowski

Summary

Affiliation: Institute of Biochemistry and Biophysics
Country: Poland

Publications

  1. ncbi request reprint A single subunit, Dis3, is essentially responsible for yeast exosome core activity
    Andrzej Dziembowski
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique UPR2167, associée à l Université Pierre et Marie Curie, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Nat Struct Mol Biol 14:15-22. 2007
  2. doi request reprint Structure of the active subunit of the yeast exosome core, Rrp44: diverse modes of substrate recruitment in the RNase II nuclease family
    Esben Lorentzen
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Mol Cell 29:717-28. 2008
  3. pmc Identification of a novel human mitochondrial endo-/exonuclease Ddk1/c20orf72 necessary for maintenance of proper 7S DNA levels
    Roman J Szczesny
    Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawinskiego 5A, 02 106 Warsaw, Poland
    Nucleic Acids Res 41:3144-61. 2013
  4. pmc The RNA exosome complex central channel controls both exonuclease and endonuclease Dis3 activities in vivo and in vitro
    Karolina Drazkowska
    Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    Nucleic Acids Res 41:3845-58. 2013
  5. pmc A new strategy for gene targeting and functional proteomics using the DT40 cell line
    Kinga P Orlowska
    Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawinskiego 5A, 02 106, Warsaw, Poland and Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    Nucleic Acids Res 41:e167. 2013
  6. doi request reprint Multiple myeloma-associated hDIS3 mutations cause perturbations in cellular RNA metabolism and suggest hDIS3 PIN domain as a potential drug target
    Rafal Tomecki
    Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland, Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawinskiego 5A, 02 106 Warsaw, Poland and International Institute of Molecular and Cell Biology, Trojdena 4, 02 109 Warsaw, Poland
    Nucleic Acids Res 42:1270-90. 2014
  7. ncbi request reprint The yeast THO complex forms a 5-subunit assembly that directly interacts with active chromatin
    Kamil Gewartowski
    Institute of Biochemistry and Biophysics Polish Academy of Sciences Warsaw, Poland Department of Genetics and Biotechnology Faculty of Biology University of Warsaw Warsaw, Poland
    Bioarchitecture 2:134-7. 2012
  8. pmc Human mitochondrial RNA decay mediated by PNPase-hSuv3 complex takes place in distinct foci
    Lukasz S Borowski
    Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, 02 106 Warsaw, Poland
    Nucleic Acids Res 41:1223-40. 2013
  9. pmc The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L
    Rafal Tomecki
    Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    EMBO J 29:2342-57. 2010
  10. pmc Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase
    Rafal Tomecki
    Department of Genetics, Warsaw University, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    Nucleic Acids Res 32:6001-14. 2004

Collaborators

Detail Information

Publications27

  1. ncbi request reprint A single subunit, Dis3, is essentially responsible for yeast exosome core activity
    Andrzej Dziembowski
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique UPR2167, associée à l Université Pierre et Marie Curie, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Nat Struct Mol Biol 14:15-22. 2007
    ..We observed that the yeast exosome ring mediates interactions with protein partners, providing an explanation for its essential function...
  2. doi request reprint Structure of the active subunit of the yeast exosome core, Rrp44: diverse modes of substrate recruitment in the RNase II nuclease family
    Esben Lorentzen
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Mol Cell 29:717-28. 2008
    ..The structure provides a molecular rationale for the observed biochemical properties of the RNase R family of nucleases...
  3. pmc Identification of a novel human mitochondrial endo-/exonuclease Ddk1/c20orf72 necessary for maintenance of proper 7S DNA levels
    Roman J Szczesny
    Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawinskiego 5A, 02 106 Warsaw, Poland
    Nucleic Acids Res 41:3144-61. 2013
    ..We propose a structural model of Ddk1 and discuss its similarity to other PD-(D/E)XK superfamily members...
  4. pmc The RNA exosome complex central channel controls both exonuclease and endonuclease Dis3 activities in vivo and in vitro
    Karolina Drazkowska
    Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    Nucleic Acids Res 41:3845-58. 2013
    ..Finally, we analysed the impact of a lethal mutation of conserved basic residues in Rrp4 cap subunit and found that it inhibits digestion of single-stranded and structured RNA substrates...
  5. pmc A new strategy for gene targeting and functional proteomics using the DT40 cell line
    Kinga P Orlowska
    Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawinskiego 5A, 02 106, Warsaw, Poland and Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    Nucleic Acids Res 41:e167. 2013
    ..We also report proof of principle results using the key proteins involved in RNA decay, namely EXOSC8, EXOSC9, CNOT7 and UPF1. ..
  6. doi request reprint Multiple myeloma-associated hDIS3 mutations cause perturbations in cellular RNA metabolism and suggest hDIS3 PIN domain as a potential drug target
    Rafal Tomecki
    Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland, Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawinskiego 5A, 02 106 Warsaw, Poland and International Institute of Molecular and Cell Biology, Trojdena 4, 02 109 Warsaw, Poland
    Nucleic Acids Res 42:1270-90. 2014
    ..It is an interesting example of intramolecular synthetic lethality with putative therapeutic potential in humans. ..
  7. ncbi request reprint The yeast THO complex forms a 5-subunit assembly that directly interacts with active chromatin
    Kamil Gewartowski
    Institute of Biochemistry and Biophysics Polish Academy of Sciences Warsaw, Poland Department of Genetics and Biotechnology Faculty of Biology University of Warsaw Warsaw, Poland
    Bioarchitecture 2:134-7. 2012
    ..Finally, we comment on THO recruitment to active chromatin, a role that is linked to mRNA biogenesis. ..
  8. pmc Human mitochondrial RNA decay mediated by PNPase-hSuv3 complex takes place in distinct foci
    Lukasz S Borowski
    Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, 02 106 Warsaw, Poland
    Nucleic Acids Res 41:1223-40. 2013
    ..Notably, interaction between PNPase and hSuv3 is essential for efficient mitochondrial RNA degradation. This provides indirect evidence that degradosome-dependent mitochondrial RNA decay takes place in foci...
  9. pmc The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L
    Rafal Tomecki
    Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    EMBO J 29:2342-57. 2010
    ..Both hDIS3 and hDIS3L are active exonucleases; however, only hDIS3 has retained endonucleolytic activity. Our data suggest that three different ribonucleases can serve as catalytic subunits for the exosome in human cells...
  10. pmc Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase
    Rafal Tomecki
    Department of Genetics, Warsaw University, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    Nucleic Acids Res 32:6001-14. 2004
    ....
  11. ncbi request reprint Human polynucleotide phosphorylase, hPNPase, is localized in mitochondria
    Jan Piwowarski
    Department of Genetics, Warsaw University and Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul Pawinskiego 5a, 02 106 Warsaw, Poland
    J Mol Biol 329:853-7. 2003
    ..Here, we demonstrate that the hPNPase is localized in mitochondria. Our finding suggests the involvement of mitochondrial RNA metabolism in cellular senescence...
  12. pmc Localisation of the human hSuv3p helicase in the mitochondrial matrix and its preferential unwinding of dsDNA
    Michal Minczuk
    Department of Genetics, University of Warsaw, Pawinskiego 5A, 02 106 Warsaw, Poland
    Nucleic Acids Res 30:5074-86. 2002
    ..The presented analysis of the hSuv3p NTPase/helicase suggests that new functions of the protein have been acquired in the course of evolution...
  13. pmc C16orf57, a gene mutated in poikiloderma with neutropenia, encodes a putative phosphodiesterase responsible for the U6 snRNA 3' end modification
    Seweryn Mroczek
    Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Poland
    Genes Dev 26:1911-25. 2012
    ..Our results predict an unexpected molecular basis for PN, DC, and RTS and provide insight into U6 snRNA 3' end formation...
  14. ncbi request reprint Catalytic properties of the eukaryotic exosome
    Aleksander Chlebowski
    Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, and Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    Adv Exp Med Biol 702:63-78. 2010
    ..Rrp6 proteins are distributive exonucleases. This chapter will review the current knowledge about the catalytic properties of theses nucleases and their interplay within the exosome holocomplex...
  15. doi request reprint Endonucleolytic RNA cleavage by a eukaryotic exosome
    Alice Lebreton
    Equipe Labellisée La Ligue, Centre de Genetique Moleculaire, CNRS UPR 2167, 91198 Gif sur Yvette, France
    Nature 456:993-6. 2008
    ..The mode of action of eukaryotic exosome cores in RNA processing and degradation should be reconsidered, taking into account the cooperation between its multiple ribonucleolytic activities...
  16. pmc Comprehensive structural and substrate specificity classification of the Saccharomyces cerevisiae methyltransferome
    Tomasz Wlodarski
    Laboratory of Bioinformatics and Systems Biology, Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw, Warsaw, Poland
    PLoS ONE 6:e23168. 2011
    ..Finally, we show that, in S. cerevisiae, methylation is carried out by 34 RNA MTases, 32 protein MTases, eight small molecule MTases, three lipid MTases, and nine MTases with still unknown substrate specificity...
  17. pmc Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing
    Andrzej Dziembowski
    Centre de Genetique Moleculaire, Avenue de la Terrasse, Gif sur Yvette, France
    EMBO J 23:4847-56. 2004
    ..The corresponding complex was named pre-mRNA REtention and Splicing (RES). The presence of RES subunit homologues in numerous eukaryotes suggests that its function is evolutionarily conserved...
  18. pmc Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism
    Rafal Tomecki
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, University of Warsaw, 02 106 Warsaw, Poland
    RNA 16:1692-724. 2010
    ..The aim of this review is to discuss the impact of all of these discoveries on our current understanding of eukaryotic RNA metabolism...
  19. doi request reprint U6 RNA biogenesis and disease association
    Seweryn Mroczek
    Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    Wiley Interdiscip Rev RNA 4:581-92. 2013
    ..This maturation step is fundamental for U6 snRNP assembly and recycling. Usb1 represents the first example of a direct association between a spliceosomal U6 snRNA biogenesis factor and human genetic disease...
  20. doi request reprint Probabilistic approach to predicting substrate specificity of methyltransferases
    Teresa Szczepińska
    Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, United States of America Institute for Translational Sciences, University of Texas Medical Branch, Galveston, Texas, United States of America Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland
    PLoS Comput Biol 10:e1003514. 2014
    ..Our approach not only allows for highly accurate prediction of functional specificity of MTases, but also provides insight into general rules governing MTase substrate specificity. ..
  21. ncbi request reprint Recent developments in the analysis of protein complexes
    Andrzej Dziembowski
    Equipe labelisée La Ligue, Centre de Genetique Moleculaire, CNRS UPR 2167 Associated with University Paris 6, Avenue de la Terrasse, 91198 Cedex, Gif sur Yvette, France
    FEBS Lett 556:1-6. 2004
    ..We suggest that the next level of analysis in this area will be the definition of internal subunit arrangement in complexes as a first step toward more detailed structural analyses...
  22. doi request reprint RNA decay machines: the exosome
    Aleksander Chlebowski
    Institute of Genetics and Biotechnology, University of Warsaw, Warsaw, Poland
    Biochim Biophys Acta 1829:552-60. 2013
    ..Finally, Part Three presents an overview of the RNA metabolism pathways that involve the exosome. This article is part of a Special Issue entitled: RNA Decay mechanisms...
  23. doi request reprint Catalytic properties of the eukaryotic exosome
    Aleksander Chlebowski
    Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland
    Adv Exp Med Biol 702:63-78. 2011
    ..Rrp6 proteins are distributive exonucleases. This chapter will review the current knowledge about the catalytic properties of theses nucleases and their interplay within the exosome holocomplex...
  24. doi request reprint Identification of protein partners in mycobacteria using a single-step affinity purification method
    Przemysław Płociński
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
    PLoS ONE 9:e91380. 2014
    ..Dynamic changes in the composition of protein complexes under induction of UV damage were observed when UvrA-eGFP expressing cells treated with UV light were used to co-purify UvrA interaction partners. ..
  25. ncbi request reprint The yeast mitochondrial degradosome. Its composition, interplay between RNA helicase and RNase activities and the role in mitochondrial RNA metabolism
    Andrzej Dziembowski
    Department of Genetics, Warsaw University and Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    J Biol Chem 278:1603-11. 2003
    ..We suggest that the degradosome is a central part of a mitochondrial RNA surveillance system responsible for degradation of aberrant and unprocessed RNAs...
  26. pmc RNA channelling by the archaeal exosome
    Esben Lorentzen
    European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117, Heidelberg, Germany
    EMBO Rep 8:470-6. 2007
    ..Multiple substrate-binding sites and the entrapment of the substrate in the central channel provide a rationale for the processive degradation of extended RNAs and the stalling of structured RNAs...
  27. pmc Subunit architecture of multimeric complexes isolated directly from cells
    Helena Hernandez
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    EMBO Rep 7:605-10. 2006
    ....