Miroslav Pohanka

Summary

Publications

  1. ncbi request reprint Biochemical insight into soman intoxication and treatment with atropine, HI-6, trimedoxime, and K203 in a rat model
    M Pohanka
    Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
    Bratisl Lek Listy 112:539-44. 2011
  2. doi request reprint Copper, aluminum, iron and calcium inhibit human acetylcholinesterase in vitro
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, CZ 500 01 Hradec Kralove, Czech Republic Karel English College in Brno, Sujanovo namesti 356 1, 60200 Brno, Czech Republic Electronic address
    Environ Toxicol Pharmacol 37:455-9. 2014
  3. ncbi request reprint Tacrine alters antibodies level in Francisella tularensis-infected mice
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Neuro Endocrinol Lett 34:134-7. 2013
  4. doi request reprint Voltammetric assay of butyrylcholinesterase in plasma samples and its comparison to the standard spectrophotometric test
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, CZ 500 01 Hradec Kralove, Czech Republic Karel English College in Brno, Sujanovo namesti 356 1, 60200 Brno, Czech Republic Electronic address
    Talanta 119:412-6. 2014
  5. ncbi request reprint Alzheimer´s disease and oxidative stress: a review
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 50001 Hradec Kralove, Czech Republic
    Curr Med Chem 21:356-64. 2013
  6. doi request reprint Role of oxidative stress in infectious diseases. A review
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 50001, Hradec Kralove, Czech Republic
    Folia Microbiol (Praha) 58:503-13. 2013
  7. doi request reprint HI-6 modulates immunization efficacy in a BALB/c mouse model
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 50001 Hradec Kralove, Czech Republic Karel Englis College in Brno, Sujanovo namesti 356 1, 60200 Brno, Czech Republic Electronic address
    Environ Toxicol Pharmacol 36:801-6. 2013
  8. pmc An acetylcholinesterase-based chronoamperometric biosensor for fast and reliable assay of nerve agents
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Sensors (Basel) 13:11498-506. 2013
  9. pmc Lead toxicosis of captive vultures: case description and responses to chelation therapy
    Jiri Pikula
    Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic
    BMC Vet Res 9:11. 2013
  10. doi request reprint Sulfur mustard causes oxidative stress and depletion of antioxidants in muscles, livers, and kidneys of Wistar rats
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Drug Chem Toxicol 36:270-6. 2013

Detail Information

Publications68

  1. ncbi request reprint Biochemical insight into soman intoxication and treatment with atropine, HI-6, trimedoxime, and K203 in a rat model
    M Pohanka
    Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
    Bratisl Lek Listy 112:539-44. 2011
    ..The present experiment is based on biochemical assessment of nerve agent soman intoxication and atropine, respectively atropine and HI-6, trimedoxime or K203 treatment in rats...
  2. doi request reprint Copper, aluminum, iron and calcium inhibit human acetylcholinesterase in vitro
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, CZ 500 01 Hradec Kralove, Czech Republic Karel English College in Brno, Sujanovo namesti 356 1, 60200 Brno, Czech Republic Electronic address
    Environ Toxicol Pharmacol 37:455-9. 2014
    ..Though the inhibition is much weaker when compared to e.g. drugs with noncompetitive mechanism of action, biological relevance of the findings can be anticipated. ..
  3. ncbi request reprint Tacrine alters antibodies level in Francisella tularensis-infected mice
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Neuro Endocrinol Lett 34:134-7. 2013
    ..It is hypothesized that the cholinergic anti-inflammatory pathway is a common mechanism how inhibitors of AChE can influence immunity. Here, tularemia is used as a model disease for experimental purposes...
  4. doi request reprint Voltammetric assay of butyrylcholinesterase in plasma samples and its comparison to the standard spectrophotometric test
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, CZ 500 01 Hradec Kralove, Czech Republic Karel English College in Brno, Sujanovo namesti 356 1, 60200 Brno, Czech Republic Electronic address
    Talanta 119:412-6. 2014
    ..In a conclusion, the voltammetric assay seems to be reliable and suitable for routine performance...
  5. ncbi request reprint Alzheimer´s disease and oxidative stress: a review
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 50001 Hradec Kralove, Czech Republic
    Curr Med Chem 21:356-64. 2013
    ..The disease progression is enhanced by oxidative stress. However, the role of oxidative stress is not universally accepted. The current review covers and discusses the basic evidence and role of oxidative stress in AD development. ..
  6. doi request reprint Role of oxidative stress in infectious diseases. A review
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 50001, Hradec Kralove, Czech Republic
    Folia Microbiol (Praha) 58:503-13. 2013
    ..This article reviews the basic pathways in which reactive species can accumulate during infectious diseases and discusses the related health consequences. ..
  7. doi request reprint HI-6 modulates immunization efficacy in a BALB/c mouse model
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 50001 Hradec Kralove, Czech Republic Karel Englis College in Brno, Sujanovo namesti 356 1, 60200 Brno, Czech Republic Electronic address
    Environ Toxicol Pharmacol 36:801-6. 2013
    ..The findings point at the suitability of HI-6 for improving vaccination efficacy at the level of immunity regulation by the nervous system. ..
  8. pmc An acetylcholinesterase-based chronoamperometric biosensor for fast and reliable assay of nerve agents
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Sensors (Basel) 13:11498-506. 2013
    ..The assay was reliable, with minor interferences caused by the organic solvents ethanol, methanol, isopropanol and acetonitrile. Isopropanol was chosen as suitable medium for processing lipophilic samples...
  9. pmc Lead toxicosis of captive vultures: case description and responses to chelation therapy
    Jiri Pikula
    Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic
    BMC Vet Res 9:11. 2013
    ..This study reports on experience with accidental lead intoxication and responses to chelation therapy in captive Cinereous (Aegypius monachus) and Egyptian (Neophron percnopterus) Vultures...
  10. doi request reprint Sulfur mustard causes oxidative stress and depletion of antioxidants in muscles, livers, and kidneys of Wistar rats
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Drug Chem Toxicol 36:270-6. 2013
    ..Significant OS, depletion of low-molecular-mass antioxidants, increase in caspase activity, and some other processes related to SM action were determined. Moreover, we infer a principal role of OS in the tested organs...
  11. pmc Caffeine inhibits acetylcholinesterase, but not butyrylcholinesterase
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 50001 Hradec Kralove, Czech Republic
    Int J Mol Sci 14:9873-82. 2013
    ..The biological relevance of the findings is discussed...
  12. doi request reprint Spectrophotometric methods based on 2,6-dichloroindophenol acetate and indoxylacetate for butyrylcholinesterase activity assay in plasma
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 50001 Hradec Kralove, Czech Republic
    Talanta 106:281-5. 2013
    ..All methods were verified using pooled human plasma samples and tested for potential interferents. 2,6-dichloroindophenol acetate is recommended as suitable substrate for BChE assay in clinical diagnostics...
  13. ncbi request reprint Melatonin influences antioxidant homeostasis and basal metabolism in the BALB/c mouse model
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Neuro Endocrinol Lett 33:183-9. 2012
    ..Though there is evidence of beneficial effects of melatonin, the substance was not investigated in greater details associated with specific regulation of oxidative stress in organs and tissues...
  14. pmc Galantamine effect on tularemia pathogenesis in a BALB/c mouse model
    Miroslav Pohanka
    University of Defence, Trebesska, Hradec Kralove, Czech Republic
    Iran Biomed J 16:156-61. 2012
    ..Owing to the significant role of cholinergic anti-inflammatory pathways in neuro-immunomodulation, we decided to examine the effect of galantamine on tularemia-infected BALB/c mice...
  15. doi request reprint Acetylcholinesterase inhibitors: a patent review (2008 - present)
    Miroslav Pohanka
    University of Defense, Faculty of Military Health Sciences, Trebesska 1575, 50001 Hradec Kralove, Czech Republic
    Expert Opin Ther Pat 22:871-86. 2012
    ..Beside toxins, drugs for treatment of Alzheimer's disease and myasthenia gravis, such as galantamine, donepezil, rivastigmine, tacrine, huperzine, pyridostigmine and neostigmine, are known...
  16. doi request reprint Tularemia progression accompanied with oxidative stress and antioxidant alteration in spleen and liver of BALB/c mice
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01, Hradec Kralove, Czech Republic
    J Microbiol 50:401-8. 2012
    ..The demonstrated effects of tularemia-induced pathology had a more extensive impact on the liver than on the spleen...
  17. ncbi request reprint Antioxidants countermeasures against sulfur mustard
    M Pohanka
    Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 50001 Hradec Kralove, Czech Republic
    Mini Rev Med Chem 12:742-8. 2012
    ..The suitability of these antioxidants for therapy purposes is considered in a separate chapter. The review concludes with a view to the future and the studies needed on antioxidant therapy as a countermeasure to SM poisoning...
  18. pmc Alpha7 nicotinic acetylcholine receptor is a target in pharmacology and toxicology
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 50001 Hradec Kralove, Czech Republic E Mail Tel 420 973253091
    Int J Mol Sci 13:2219-38. 2012
    ..Pharmacological, toxicological, and medicinal significance of α7 nAChR are discussed throughout this paper...
  19. ncbi request reprint Huperzine induces alteration in oxidative balance and antioxidants in a guinea pig model
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Neuro Endocrinol Lett 32:95-100. 2011
    ..As huperzine is a promising compound for AD treatment, our study was aimed at evaluating its pertinent implications in oxidative stress...
  20. doi request reprint Toxicological scoring of Alzheimer's disease drug huperzine in a guinea pig model
    Miroslav Pohanka
    University of Defence, Hradec Kralove, Czech Republic
    Toxicol Mech Methods 22:231-5. 2012
    ..Effects on liver, kidney and spleen were milder. We discuss ambivalent action of huperzine in the body and judge the huperzine action owing to recently reported experiments...
  21. ncbi request reprint Ascorbic acid: an old player with a broad impact on body physiology including oxidative stress suppression and immunomodulation: a review
    M Pohanka
    University of Defence, Trebesska, Hradec Kralove, Czech Republic
    Mini Rev Med Chem 12:35-43. 2012
    ..The primary intention was to consider ascorbic acid not only as an antioxidant but also as a chemical compound affecting multiple pathways with a potential beneficial impact in many diseases and processes in human body...
  22. doi request reprint Cholinesterases, a target of pharmacology and toxicology
    Miroslav Pohanka
    University of Defense, Hradec Kralove, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 155:219-29. 2011
    ..Cholinesterase inhibitors are substances, either natural or man-made that interfere with the break-down of ACh and prolong its action. Hence their relevance to toxicology and pharmacology...
  23. pmc Mycoplasma gallisepticum infection in the grey partridge Perdix perdix: outbreak description, histopathology, biochemistry and antioxidant parameters
    Frantisek Vitula
    Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
    BMC Vet Res 7:34. 2011
    ..The objective of this study was to describe the outbreak, pathology, and blood and tissue biochemical responses in a flock of grey partridges naturally infected with Mycoplasma gallisepticum...
  24. ncbi request reprint Modulation of ionising radiation generated oxidative stress by HI-6 (asoxime) in a laboratory rat model
    Miroslav Pohanka
    Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Neuro Endocrinol Lett 31:62-8. 2010
    ..However, the present experiments were carried out in order to assess the antioxidant effect of HI-6 in irradiated animals...
  25. doi request reprint Oxidative stress after sulfur mustard intoxication and its reduction by melatonin: efficacy of antioxidant therapy during serious intoxication
    Miroslav Pohanka
    Center of Advanced Studies, University of Defence Trebesska 1575, Hradec Kralove, Czech Republic
    Drug Chem Toxicol 34:85-91. 2011
    ..The carbonyl levels were significantly increased due to SM, and the carbonylation was slowed due to melatonin intake. In conclusion, melatonin seems to be a prospective compound in reducing SM toxicity impact in the rat...
  26. doi request reprint Investigation of oxidative stress in blood, brain, kidney, and liver after oxime antidote HI-6 application in a mouse experimental model
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
    Drug Chem Toxicol 34:255-60. 2011
    ..HI-6 potential to produce nephropathy is hypothesized. The achieved conclusions were quite surprising and showed a complex impact of HI-6 on the body...
  27. doi request reprint Asoxime (HI-6) impact on dogs after one and tenfold therapeutic doses: assessment of adverse effects, distribution, and oxidative stress
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
    Environ Toxicol Pharmacol 32:75-81. 2011
    ..Blood samples were collected before HI-6 application and after 30, 60, and 120 min. The overall HI-6 impact on organism is discussed...
  28. doi request reprint Metrifonate alters antioxidant levels and caspase activity in cerebral cortex of Wistar rats
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska, Hradec Kralove, Czech Republic
    Toxicol Mech Methods 21:585-90. 2011
    ..Cerebral cortex tissues had decreased AChE and increased caspase 3 activities as well as the FRAP level. Owing to the novel findings, suitability of metrifonate for AD therapy is discussed...
  29. doi request reprint Sulfur mustard induced oxidative stress and its alteration by epigallocatechin gallate
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 50001 Hradec Kralove, Czech Republic
    Toxicol Lett 201:105-9. 2011
    ..The data also appoints at difficulty in antioxidant therapy as prophylaxis to the oxidative stress related toxins exposure and ambivalent modulation of oxidative stress...
  30. ncbi request reprint Macrophage-assisted inflammation and pharmacological regulation of the cholinergic anti-inflammatory pathway
    M Pohanka
    Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 50001 Hradec Kralove, Czech Republic
    Curr Med Chem 18:539-51. 2011
    ....
  31. ncbi request reprint Novel acetylcholinesterase reactivator--oxime K048--reactivation activity in vitro
    Kamil Kuca
    Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Med Chem 6:1-5. 2010
    ..At a concentration of 10(-5) M, which is more common for human use, only methylchlorpyrifos-inhibited AChE was reactivated...
  32. doi request reprint Monooxime-monocarbamoyl Bispyridinium Xylene-Linked Reactivators of Acetylcholinesterase-Synthesis, In vitro and Toxicity Evaluation, and Docking Studies
    Kamil Musilek
    Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, Hradec Kralove, Czech Republic
    ChemMedChem 5:247-54. 2010
    ..The structure-activity relationship (SAR) study confirmed the apparent influence of the xylene linkage and carbamoyl moiety on the reactivation ability and toxicity of the agents...
  33. doi request reprint Effect of five acetylcholinesterase reactivators on tabun-intoxicated rats: induction of oxidative stress versus reactivation efficacy
    Miroslav Pohanka
    Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
    J Appl Toxicol 29:483-8. 2009
    ..The oxidative stress provoked by HI-6 and K628 was found to be significant on probability level P = 0.05. The others reactivators did not affect antioxidant levels...
  34. doi request reprint Changes of rat plasma total low molecular weight antioxidant level after tabun exposure and consequent treatment by acetylcholinesterase reactivators
    Miroslav Pohanka
    Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    J Enzyme Inhib Med Chem 26:93-7. 2011
    ..The principle of oxime reactivator function and a novel insight into AChE activity regulation and oxidative stress is discussed...
  35. doi request reprint The preparation, in vitro screening and molecular docking of symmetrical bisquaternary cholinesterase inhibitors containing a but-(2E)-en-1,4-diyl connecting linkage
    Kamil Musilek
    Department of Toxicology, University of Defence, Faculty of Military Health Sciences, Trebesska 1575, Hradec Kralove, Czech Republic
    J Enzyme Inhib Med Chem 26:245-53. 2011
    ..Consequently, the docking results confirmed the apparent π-π or π-cationic interactions with the key amino acid residues of hAChE and the binding of the chosen compound at the enzyme catalytic site...
  36. doi request reprint Preparation and in vitro screening of symmetrical bis-isoquinolinium cholinesterase inhibitors bearing various connecting linkage--implications for early Myasthenia gravis treatment
    Kamil Musilek
    University of Defence, Faculty of Military Health Sciences, Department of Toxicology, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
    Eur J Med Chem 46:811-8. 2011
    ..Two newly prepared compounds were also chosen for docking studies that confirmed apparent π-π or π-cationic interactions aside the cholinesterases catalytic sites. The SAR findings were discussed...
  37. doi request reprint Preparation, in vitro screening and molecular modelling of symmetrical 4-tert-butylpyridinium cholinesterase inhibitors--analogues of SAD-128
    Kamil Musilek
    University of Defence, Faculty of Military Health Sciences, Department of Toxicology, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
    Bioorg Med Chem Lett 21:150-4. 2011
    ..The SAR was stated from obtained data. The former receptor studies were reported and discussed. The further in vivo studies were recommended in the view of OP pre-exposure treatment...
  38. doi request reprint Mono-oxime bisquaternary acetylcholinesterase reactivators with prop-1,3-diyl linkage-Preparation, in vitro screening and molecular docking
    Kamil Musilek
    University of Defence, Hradec Kralove, Czech Republic
    Bioorg Med Chem 19:754-62. 2011
    ..The SAR features concerning the non-oxime part of the reactivator molecule are also discussed...
  39. doi request reprint Synthesis and in vitro evaluation of N-alkyl-7-methoxytacrine hydrochlorides as potential cholinesterase inhibitors in Alzheimer disease
    Jan Korabecny
    Charles University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry and Drug Control, Hradec Kralove, Czech Republic
    Bioorg Med Chem Lett 20:6093-5. 2010
    ..Three novel compounds showed promising results towards hAChE better to THA or 7-MEOTA. Three compounds resulted as potent inhibitors of hBChE. The SAR findings highlighted the C(6)-C(7)N-alkyl chains for cholinesterase inhibition...
  40. doi request reprint Preparation and in vitro screening of symmetrical bispyridinium cholinesterase inhibitors bearing different connecting linkage-initial study for Myasthenia gravis implications
    Kamil Musilek
    Department of Toxicology, Faculty of Military Health Sciences, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
    Bioorg Med Chem Lett 20:1763-6. 2010
    ..The kinetics assay confirmed non-competitive inhibition of AChE by two best newly prepared compounds...
  41. ncbi request reprint The effect of HI-6 on cholinesterases and on the cholinergic system of the rat bladder
    Ondrej Soukup
    Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Neuro Endocrinol Lett 29:759-62. 2008
    ..Oximes can react - except their reactivating effect on cholinesterases - directly with cholinoreceptors. HI-6 is an oxime that may have an inhibitory effect on the muscarinic receptors, too...
  42. doi request reprint In vitro reactivation of trichlorfon-inhibited butyrylcholinesterase using HI-6, obidoxime, pralidoxime and K048
    Miroslav Pohanka
    Faculty of Military Health Sciences, Center of Advanced Studies, University of Defence, Hradec Kralove, Czech Republic
    J Enzyme Inhib Med Chem 24:680-3. 2009
    ..There was also an observed lowering of reactivation efficacy when butyrylcholinesterase was exposed to trichlorfon for a longer time interval...
  43. doi request reprint Oxime K027: novel low-toxic candidate for the universal reactivator of nerve agent- and pesticide-inhibited acetylcholinesterase
    Kamil Kuca
    Center of Advanced Studies, Faculty of Military Health Sciences, University of Defense, Trebesska, Hradec Kralove, Czech Republic
    J Enzyme Inhib Med Chem 25:509-12. 2010
    ..In the case of cyclosarin- and soman-inhibited AChE, oxime K027 did not reach sufficient reactivation potency...
  44. ncbi request reprint Tacrine is implicated in oxidative stress in the laboratory guinea pig model
    Alzbeta Kracmarova
    Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Neuro Endocrinol Lett 33:136-44. 2012
    ..The hepatotoxicity can be decreased by alternative ways of drug administration avoiding thus the first pass effect. The present study is aimed to investigate the influence of intramuscularly administrated tacrine on oxidative stress...
  45. doi request reprint Novel bisquaternary oximes--reactivation of acetylcholinesterase and butyrylcholinesterase inhibited by paraoxon
    Kamil Kuca
    Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Molecules 14:4915-21. 2009
    ..In case of BuChE reactivation, two compounds (K053 and K068) achieved similar results as obidoxime...
  46. doi request reprint Passive diffusion of acetylcholinesterase oxime reactivators through the blood-brain barrier: influence of molecular structure
    Jana Zdarova Karasova
    Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Toxicol In Vitro 24:1838-44. 2010
    ....
  47. doi request reprint Reactivation of human brain homogenate cholinesterases inhibited by Tabun using newly developed oximes K117 and K127
    Kamil Kuca
    Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, 500 01 Hradec Kralove, Czech Republic
    Basic Clin Pharmacol Toxicol 105:207-10. 2009
    ..Due to this, experiments on human tissue should be conducted after in vitro and in vivo tests on animals to eliminate such important failures of promising oximes...
  48. ncbi request reprint New performance of biosensor technology for Alzheimer's disease drugs: in vitro comparison of tacrine and 7-methoxytacrine
    Miroslav Pohanka
    Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Neuro Endocrinol Lett 29:755-8. 2008
    ..Our study shows that biosensor technology could be used as a quick and cheap tool for testing of promising AChE inhibitors (AD drug candidates)...
  49. doi request reprint Preparation of the pyridinium salts differing in the length of the N-alkyl substituent
    Jan Marek
    Faculty of Military Health Science, University of Defence, Hradec Kralove, 50001, Czech Republic
    Molecules 15:1967-72. 2010
    ..Additionally, HPLC technique was established to distinguish each prepared pyridinium analogue. This study represents universal method for preparation and identification of quaternary pyridinium detergents...
  50. ncbi request reprint Assessment of low-molecular-weight antioxidants in Francisella tularensis infected hosts: comparison of two rodents with different susceptibility to tularemia
    Miroslav Pohanka
    Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Neuro Endocrinol Lett 30:186-91. 2009
    ..The pathogenesis of tularemia is still not fully understood. The main pathway is associated with proliferation in macrophages after activation by reactive oxygen species in phagosomes...
  51. ncbi request reprint Progress of biosensors based on cholinesterase inhibition
    Miroslav Pohanka
    Center of Advanced Studies, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, Hradec Kralove, Czech Republic
    Curr Med Chem 16:1790-8. 2009
    ..This review is focused on novel trends within such biosensors as a result of the known platform...
  52. ncbi request reprint Reactivation of VX-inhibited AChE by novel oximes having two oxygen atoms in the linker
    Kamil Kuca
    Center of Advanced Studies, Hradec Kralove, Czech Republic Department of Toxicology, Faculty of Military Health Sciences, Hradec Kralove, Czech Republic
    Environ Toxicol Pharmacol 30:85-7. 2010
    ..Hence, these oximes may be worthy of future development for the treatment of nerve agent intoxications, especially, with lipophilic agents such as soman and cyclosarin...
  53. ncbi request reprint Reactivation potency of the acetylcholinesterase reactivator obidoxime is limited
    Kamil Kuca
    Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 153:259-62. 2009
    ..It is also needed by the fact that clinical findings considering this oxime are controversial...
  54. doi request reprint Colorimetric dipstick for assay of organophosphate pesticides and nerve agents represented by paraoxon, sarin and VX
    Miroslav Pohanka
    Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
    Talanta 81:621-4. 2010
    ..The achieved limit of detection was 5 x 10(-8)M for paraoxon-ethyl and 5 x 10(-9)M for sarin and VX. Dipsticks were found stable for at least one month. Suitability of these dipsticks for routine assay is discussed...
  55. ncbi request reprint Evaluation of aflatoxin B1--acetylcholinesterase dissociation kinetic using the amperometric biosensor technology: prospect for toxicity mechanism
    Miroslav Pohanka
    Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 50001 Hradec Kralove, Czech Republic
    Protein Pept Lett 17:340-2. 2010
    ..0005 s(-1). The half time (t(1/2)) of complex dissociation was 146 s. The achieved data appoint at fact that AChE could allow to distribute aflatoxins in body instead acting as a scavenger. Analytical impact of study is discussed, too...
  56. pmc Effect of seven newly synthesized and currently available oxime cholinesterase reactivators on cyclosarin-intoxicated rats
    Jana Zdarova Karasova
    Department of Toxicology, Faculty of Military Health Sciences, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
    Int J Mol Sci 10:3065-75. 2009
    ..Values for newly synthesized oximes were less than 10% for K206, K269 and K203...
  57. ncbi request reprint Chemical warfare agents
    Kamil Kuca
    Center of Advanced Studies and Department of Toxicology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
    EXS 100:543-58. 2010
    ..This chapter briefly introduces the separate groups of chemical warfare agents together with their members and the potential therapy that should be applied in case someone is intoxicated by these agents...
  58. ncbi request reprint Prophylaxis and Post-exposure Treatment of Intoxications Caused by Nerve Agents and Organophosphorus Pesticides
    Kamil Kuca
    Center of Advanced Studies, Faculty of Military Health Sciences, Trebesska 1575, 50001 Hradec Kralove, Czech Republic
    Mini Rev Med Chem 13:2102-15. 2013
    ....
  59. pmc Evaluation of cholinesterase activities during in vivo intoxication using an electrochemical sensor strip - correlation with intoxication symptoms
    Miroslav Pohanka
    Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic E Mail L N
    Sensors (Basel) 9:3627-34. 2009
    ..The minimal cholinesterase activity essential to survival is around 10%, when compared with the initial state. No changes in levels of low moleculary weight antioxidants were observed...
  60. doi request reprint Shift of oxidants and antioxidants levels in rats as a reaction to exposure to sulfur mustard
    Miroslav Pohanka
    Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    J Appl Toxicol 29:643-7. 2009
    ..The most surprising facts were found when the highest dose of 80 mg kg(-1) body weight of sulfur mustard was applied. While antioxidants were significantly increased, oxidants were decreased on an extensive scale...
  61. ncbi request reprint Cholinesterase biosensor construction - a review
    Miroslav Pohanka
    Center of Advanced Studies, Faculty of Military Medicine, University of Defense, Hradec Kralove, Czech Republic
    Protein Pept Lett 15:795-8. 2008
    ..In this review, some strategies convenient for biosensor construction are presented. Solutions for cholinesterase immobilization and output signal monitoring are presented as the basic presumptions for successful biosensor construction...
  62. pmc Ferric reducing antioxidant power and square wave voltammetry for assay of low molecular weight antioxidants in blood plasma: performance and comparison of methods
    Miroslav Pohanka
    Centre of Advanced Studies and Department of Toxicology, Faculty of Military Health Sciences, University of Defence Trebesska 1575, 50001 Hradec Kralove, Czech Republic
    Sensors (Basel) 9:9094-103. 2009
    ..The methods employed in the study were advantageous in minimal sample volume consumption and fast acquisition of results...
  63. pmc Effect of Several New and Currently Available Oxime Cholinesterase Reactivators on Tabun-intoxicated Rats
    Jana Zdarova Karasova
    Department of Toxicology, Faculty of Military Health Sciences, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic E Mails J K K K
    Int J Mol Sci 9:2243-52. 2008
    ..Partial protection of brain ChE against tabun inhibition was observed after administration of trimedoxime (acetylcholinesterase 20%; butyrylcholinesterase 30%) and obidoxime (acetylcholinesterase 12%; butyrylcholinesterase 16%)...
  64. pmc Reactivation of human acetylcholinesterase and butyrylcholinesterase inhibited by leptophos-oxon with different oxime reactivators in vitro
    Daniel Jun
    Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Int J Mol Sci 11:2856-63. 2010
    ..The most potent reactivators of inhibited BChE were K033, obidoxime, K117, bis-3-PA, K075, K074 and K127. The reactivation efficacy of tested oximes was lower in case of leptophos-oxon inhibited BChE...
  65. pmc Acetylcholine and an acetylcholinesterase inhibitor neostigmine can aggravate tularemia progress in BALB/c mice
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
    Interdiscip Toxicol 5:21-4. 2012
    ..Administration of AChE inhibitors to the individuals suffering from tularemia is contra-indicatory. Drugs based on AChE inhibition should be restricted when tularemia or disease with a similar pathogenesis is suspected...
  66. pmc Assessment of Acetylcholinesterase Activity Using Indoxylacetate and Comparison with the Standard Ellman's Method
    Miroslav Pohanka
    Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 50001 Hradec Kralove, Czech Republic
    Int J Mol Sci 12:2631-40. 2011
    ..Thus it can be advantageously used for accurate measurement of AChE activity. Suitability of assay for butyrylcholinesterase activity assessment is also discussed...
  67. doi request reprint Improvement of acetylcholinesterase-based assay for organophosphates in way of identification by reactivators
    Miroslav Pohanka
    Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
    Talanta 77:451-4. 2008
    ..Practical impact of described method is discussed...
  68. ncbi request reprint Biological warfare agents
    Miroslav Pohanka
    Center of Advanced Studies and Department of Toxicology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
    EXS 100:559-78. 2010
    ..The last section tries to estimate future trends in research on biological warfare agents...