Defining features and exploring chemical modifications to manipulate RNAa activityRobert F Place
Helen Diller Comprehensive Cancer Center, Department of Urology, University of California, PO Box 589001, San Francisco, CA 94158 9001, USA
Curr Pharm Biotechnol 11:518-26. 2010
..These findings reveal functional features of RNAa that may be utilized to augment saRNA function for mechanistic studies or the development of RNAa-based drugs...
- MicroRNA-373 induces expression of genes with complementary promoter sequences
Robert F Place
Department of Urology, Veterans Affairs Medical Center and University of California, San Francisco, CA 94121, USA
Proc Natl Acad Sci U S A 105:1608-13. 2008
..In conclusion, we have identified a miRNA that targets promoter sequences and induces gene expression. These findings reveal a new mode by which miRNAs may regulate gene expression...
- HDACs and the senescent phenotype of WI-38 cells
Robert F Place
Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, USA
BMC Cell Biol 6:37. 2005
..Here we examine the influence of HDAC inhibitors on the expression of senescent markers in pre- and post-senescent WI-38 cells...
Genistein induces the p21WAF1/CIP1 and p16INK4a tumor suppressor genes in prostate cancer cells by epigenetic mechanisms involving active chromatin modificationShahana Majid
Department of Urology, Veterans Affairs Medical Center, University of California, San Francisco, California 94121, USA
Cancer Res 68:2736-44. 2008
..Altogether, our data provide new insights into the epigenetic mechanism of the action of genistein that may contribute to the chemopreventive activity of this dietary isoflavone and have important implications for epigenetic therapy...
- Hsp70B' regulation and function
Emily J Noonan
Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269 3125, USA
Cell Stress Chaperones 12:393-402. 2007
..These findings are likely to be important in pathological conditions in which Hsp70B' contributes to cell survival...
- Double stranded-RNA-mediated activation of P21 gene induced apoptosis and cell cycle arrest in renal cell carcinoma
Jared M Whitson
University of California San Francisco, Department of Urology, San Francisco, CA 94121, USA
Int J Cancer 125:446-52. 2009
..This is the first report that demonstrates dsRNA mediated gene activation in renal cell carcinoma and suggests that forced over-expression of p21 may lead to an increase in apoptosis through a survivin dependent mechanism...
- Hsp70B' regulation and function
Emily J Noonan
Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269 3125, USA
Cell Stress Chaperones 12:219-29. 2007
..These findings are likely to be important in pathological conditions in which Hsp70B' contributes to cell survival...
- Upregulation of Cyclin B1 by miRNA and its implications in cancer
Vera Huang
Department of Urology and Helen Diller Comprehensive Cancer Center, University of California San Francisco, San Francisco, CA 94158, USA
Nucleic Acids Res 40:1695-707. 2012
..Such phenotypes were recapitulated by overexpression of Ccnb1. Our findings reveal an endogenous system by which miRNA functions to activate Ccnb1 expression in mouse cells and manipulate in vivo tumor development/growth...
- Prognostic value and function of KLF4 in prostate cancer: RNAa and vector-mediated overexpression identify KLF4 as an inhibitor of tumor cell growth and migration
Ji Wang
Department of Urology and Helen Diller Comprehensive Cancer Center, University of California, San Francisco, San Francisco, California, USA
Cancer Res 70:10182-91. 2010
..These results suggest that KLF4 functions as an inhibitor of tumor cell growth and migration in prostate cancer and decreased expression has prognostic value for predicting prostate cancer metastasis...
- Antitumor effect of dsRNA-induced p21(WAF1/CIP1) gene activation in human bladder cancer cells
Zhong Chen
Department of Urology, University of California San Francisco, San Francisco, CA 94143, USA
Mol Cancer Ther 7:698-703. 2008
..In conclusion, p21 activation by RNAa has antitumor activity in vitro in bladder cancer cells. These results suggest that RNAa could be used for cancer treatment by targeted activation of tumor suppressor genes...
Epigenetic modifications of RASSF1A gene through chromatin remodeling in prostate cancerKen Kawamoto
Department of Urology, Veterans Affairs Medical Center and University of California School of Medicine, San Francisco, California 94121, USA
Clin Cancer Res 13:2541-8. 2007
..The present study was designed to investigate the mechanisms of inactivation of the RASSF1A gene through the analysis of CpG methylation and histone acetylation and H3 methylation associated with the RASSF1A promoter region...
- Chromatin changes on the GSTP1 promoter associated with its inactivation in prostate cancer
Steven T Okino
Department of Urology, San Francisco Veterans Affairs Medical Center and the University of California San Francisco, San Francisco, California 94121, USA
Mol Carcinog 46:839-46. 2007
..We conclude that, in the process of prostate carcinogenesis, activating histone modifications on GSTP1 are lost and the DNA becomes methylated and inaccessible resulting in transcriptional silencing...
- Small dsRNAs induce transcriptional activation in human cells
Long Cheng Li
Department of Urology, Veterans Affairs Medical Center and University of California, San Francisco, CA 94121, USA
Proc Natl Acad Sci U S A 103:17337-42. 2006
..These findings reveal a more diverse role for small RNA molecules in the regulation of gene expression than previously recognized and identify a potential therapeutic use for dsRNA in targeted gene activation...
- Induction of NANOG expression by targeting promoter sequence with small activating RNA antagonizes retinoic acid-induced differentiation
Xiaoling Wang
Department of Urology, University of California San Francisco, San Francisco, CA 94158, USA
Biochem J 443:821-8. 2012
..Ectopic overexpression of NANOG via lentiviral transduction further recapitulates saRNA results, providing proof-of-concept that RNAa may be utilized to activate development-related genes and manipulate cell fate...
- HDAC inhibition prevents NF-kappa B activation by suppressing proteasome activity: down-regulation of proteasome subunit expression stabilizes I kappa B alpha
Robert F Place
Department of Molecular and Cellular Biology, University of Connecticut, 91 North Eagleville Road, U-3125, Storrs, CT 06269-3125, USA
Biochem Pharmacol 70:394-406. 2005
..This information may be useful for the further development and targeting of HDAC inhibitors as anti-neoplastic and anti-inflammatory agents...
- Cell number-dependent regulation of Hsp70B' expression: evidence of an extracellular regulator
Emily J Noonan
Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, USA
J Cell Physiol 210:201-11. 2007
....
- miR-449a causes Rb-dependent cell cycle arrest and senescence in prostate cancer cells
Emily J Noonan
Center for Molecular Biology in Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA
Oncotarget 1:349-58. 2010
..These data indicate that miR-449a is a miRNA component of the Rb pathway and its tumor suppressor-like effects, in part, depends on Rb status in prostate cancer cells...
- Intravesical delivery of small activating RNA formulated into lipid nanoparticles inhibits orthotopic bladder tumor growth
Moo Rim Kang
Department of Urology and Helen Diller Comprehensive Cancer Center, University of California, San Francisco, California, USA
Cancer Res 72:5069-79. 2012
..Our results provide preclinical proof-of-concept for a novel method to treat bladder cancer by intravesical administration of LNP-formulated RNA duplexes...
- RNAa is conserved in mammalian cells
Vera Huang
Helen Diller Comprehensive Cancer Center, University of California San Francisco, San Francisco, California, USA
PLoS ONE 5:e8848. 2010
..Thus far, RNAa has only been demonstrated in human cells and is unclear whether it is conserved in other mammals...
- Induced stabilization of IkappaBalpha can facilitate its re-synthesis and prevent sequential degradation
Robert F Place
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269, USA
J Cell Physiol 195:470-8. 2003
..We propose that cytokine stabilization of newly synthesized IkappaBalpha in some cell types is a critical homeostatic mechanism that limits inflammatory gene expression...
