The plurifunctional nucleolusT Pederson
Worcester Foundation for Biomedical Research and Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester Foundation Campus, 222 Maple Avenue, Shrewsbury, MA 01545, USA
Nucleic Acids Res 26:3871-6. 1998
..The plurifunctional nucleolus concept has a strong footing in contemporary data and adds a new perspective to our current picture of the spatial-functional design of the cell nucleus...
Thinking about a nuclear matrixT Pederson
Worcester Foundation for Biomedical Research, Shrewsbury, MA 01545, USA
J Mol Biol 277:147-59. 1998
..Important new clues are now coming in from promising contemporary lines of research that report on nuclear structure in living cells...
Crosslinking of hnRNP proteins to pre-mRNA requires U1 and U2 snRNPsS H Mayrand
Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545
Nucleic Acids Res 18:3307-18. 1990
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Human cell lines expressing hormone regulated T7 RNA polymerase localized at distinct intranuclear sitesS V Yarovoi
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 377 Plantation Street, Worcester, MA 01605, USA
Gene 275:73-81. 2001
..Both of these observed intranuclear localizations have relevance to the potential applications of this system...
Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cellsJ C Politz
Worcester Foundation for Biomedical Research, University of Massachusetts Medical Center, Worcester Foundation Campus, Shrewsbury, MA 01545, USA
Proc Natl Acad Sci U S A 95:6043-8. 1998
..These results demonstrate that oligos can move about within the nucleus at rates comparable to those in aqueous solution and further suggest that this is true for large ribonucleoprotein complexes as well...
Evidence for an association between U1 RNA and interspersed repeat single-copy RNAs in the cytoplasm of sea urchin eggsS Ruzdijic
Cell Bilogy Group, Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545
Development 101:107-16. 1987
..These results raise the possibility that maternal interspersed RNAs include unprocessed pre-messenger RNA molecules in arrested complexes with splicing cofactors...
Movement and localization of RNA in the cell nucleusT Pederson
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01605, USA
FASEB J 13:S238-42. 1999
..The methods we have developed and these initial results represent the first major step toward a comprehensive understanding of RNA traffic in the cell nucleus...
Half a century of "the nuclear matrix"T Pederson
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Mol Biol Cell 11:799-805. 2000
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Movement of nuclear poly(A) RNA throughout the interchromatin space in living cellsJ C Politz
Department of Biochemistry and Molecular Biology, Biomedical Imaging Center, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Curr Biol 9:285-91. 1999
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The Mr 70,000 protein of the U1 small nuclear ribonucleoprotein particle binds to the 5' stem-loop of U1 RNA and interacts with Sm domain proteinsJ R Patton
Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545
Proc Natl Acad Sci U S A 85:747-51. 1988
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Fluorescent RNA cytochemistry: tracking gene transcripts in living cellsT Pederson
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 377 Plantation Street, Worcester, MA 01605, USA
Nucleic Acids Res 29:1013-6. 2001
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Signal recognition particle components in the nucleolusJ C Politz
Department of Biochemistry, University of Massachusetts Medical School, 377 Plantation Street, Suite 337, Worcester, MA 01605, USA
Proc Natl Acad Sci U S A 97:55-60. 2000
..Our findings support the notion that the nucleolus is the site of assembly and/or interaction between the family of ribonucleoproteins involved in protein synthesis, in addition to ribosomes themselves...
Serine/threonine phosphorylation regulates binding of C hnRNP proteins to pre-mRNAS H Mayrand
Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545
Proc Natl Acad Sci U S A 90:7764-8. 1993
..These results indicate that the participation of C hnRNP proteins in pre-spliceosome assembly is coupled to a dynamic cycle of their phosphorylation and dephosphorylation...