Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometryXiangqing Pan
Department of Biology, University of Missouri, St Louis, MO, USA
Nat Protoc 5:986-92. 2010
..The method provides quantification of most major plant hormones in a single run from 50 mg of fresh plant tissue. Extraction and quantitative analysis of 40 samples takes 2-3 d...
Profiling of plant hormones by mass spectrometryXiangqing Pan
Department of Biology, University of Missouri, St Louis, MO 63121, United States
J Chromatogr B Analyt Technol Biomed Life Sci 877:2806-13. 2009
..The capacity to simultaneously quantify multiple classes of phytohormones will facilitate the study of hormone function and cross-talk...
Phospholipase Dalpha3 is involved in the hyperosmotic response in ArabidopsisYueyun Hong
Department of Biology, University of Missouri, St Louis, Missouri 63121, USA
Plant Cell 20:803-16. 2008
..These data suggest that PLDalpha3 positively mediates plant responses to hyperosmotic stresses and that increased PLDalpha3 expression and associated lipid changes promote root growth, flowering, and stress avoidance...
Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography-electrospray tandem mass spectrometryXiangqing Pan
Department of Biology, University of Missouri, St Louis, MO 63121, United States
Phytochemistry 69:1773-81. 2008
..Structurally diverse compounds from complicated biological matrices are determined with high selectivity and sensitivity...
Cytosolic glyceraldehyde-3-phosphate dehydrogenases interact with phospholipase Dδ to transduce hydrogen peroxide signals in the Arabidopsis response to stressLiang Guo
Department of Biology, University of Missouri, St Louis, Missouri 63121, USA
Plant Cell 24:2200-12. 2012
..The results indicate that the H(2)O(2)-promoted interaction of GAPC and PLDδ may provide a direct connection between membrane lipid-based signaling, energy metabolism and growth control in the plant response to ROS and water stress...
Enhancing seed quality and viability by suppressing phospholipase D in ArabidopsisShivakumar P Devaiah
Department of Biology, University of Missouri, St Louis, MO 63121, USA
Plant J 50:950-7. 2007
..A high level of PLDalpha1 is detrimental to seed quality, and attenuation of PLDalpha1 expression has the potential to improve oil stability, seed quality and seed longevity...
AtPLAI is an acyl hydrolase involved in basal jasmonic acid production and Arabidopsis resistance to Botrytis cinereaWenyu Yang
Department of Biology, University of Missouri, St Louis, Missouri 63121, USA
J Biol Chem 282:18116-28. 2007
..The study shows that AtPLAI is an acyl hydrolase, rather than a specific phospholipase A. AtPLAI is involved in basal JA production and Arabidopsis resistance to the necrotrophic fungus B. cinerea...