Richard Owczarzy

Summary

Publications

  1. pmc Design of LNA probes that improve mismatch discrimination
    Yong You
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA
    Nucleic Acids Res 34:e60. 2006
  2. pmc Measuring thermodynamic details of DNA hybridization using fluorescence
    Yong You
    Department of Molecular Genetics and Biophysics, Integrated DNA Technologies, Coralville, IA, USA
    Biopolymers 95:472-86. 2011
  3. pmc IDT SciTools: a suite for analysis and design of nucleic acid oligomers
    Richard Owczarzy
    Department of Molecular Genetics and Biophysics, Integrated DNA Technologies, Inc, 1710 Commercial Park, Coralville, IA 52241, USA
    Nucleic Acids Res 36:W163-9. 2008
  4. doi Predicting stability of DNA duplexes in solutions containing magnesium and monovalent cations
    Richard Owczarzy
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, Iowa 52241, USA
    Biochemistry 47:5336-53. 2008
  5. pmc Stability and mismatch discrimination of locked nucleic acid-DNA duplexes
    Richard Owczarzy
    Department of Molecular Genetics and Biophysics, Integrated DNA Technologies, Coralville, Iowa 52241, United States
    Biochemistry 50:9352-67. 2011
  6. ncbi Melting temperatures of nucleic acids: discrepancies in analysis
    Richard Owczarzy
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA
    Biophys Chem 117:207-15. 2005
  7. ncbi Effects of sodium ions on DNA duplex oligomers: improved predictions of melting temperatures
    Richard Owczarzy
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, Iowa 52241, USA
    Biochemistry 43:3537-54. 2004
  8. pmc Thermodynamic treatment of oligonucleotide duplex-simplex equilibria
    Richard Owczarzy
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA
    Proc Natl Acad Sci U S A 100:14840-5. 2003
  9. ncbi Effects of fluorescent dyes, quenchers, and dangling ends on DNA duplex stability
    Bernardo G Moreira
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA
    Biochem Biophys Res Commun 327:473-84. 2005
  10. doi Predicting ultraviolet spectrum of single stranded and double stranded deoxyribonucleic acids
    Andrey V Tataurov
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA
    Biophys Chem 133:66-70. 2008

Collaborators

Detail Information

Publications11

  1. pmc Design of LNA probes that improve mismatch discrimination
    Yong You
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA
    Nucleic Acids Res 34:e60. 2006
    ..New guidelines are suggested for design of LNA probes, which significantly improve mismatch discrimination in comparison with unmodified DNA probes...
  2. pmc Measuring thermodynamic details of DNA hybridization using fluorescence
    Yong You
    Department of Molecular Genetics and Biophysics, Integrated DNA Technologies, Coralville, IA, USA
    Biopolymers 95:472-86. 2011
    ..These methodological improvements will help build predictive models of nucleic acid hybridization...
  3. pmc IDT SciTools: a suite for analysis and design of nucleic acid oligomers
    Richard Owczarzy
    Department of Molecular Genetics and Biophysics, Integrated DNA Technologies, Inc, 1710 Commercial Park, Coralville, IA 52241, USA
    Nucleic Acids Res 36:W163-9. 2008
    ..The latest, most accurate thermodynamic algorithms and models are implemented. This free software is available at http://www.idtdna.com/SciTools/SciTools.aspx...
  4. doi Predicting stability of DNA duplexes in solutions containing magnesium and monovalent cations
    Richard Owczarzy
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, Iowa 52241, USA
    Biochemistry 47:5336-53. 2008
    ..When duplexes denature, some of the Mg (2+) ions associated with the DNA are released. The number of released magnesium ions per phosphate charge is sequence dependent and decreases surprisingly with increasing oligonucleotide length...
  5. pmc Stability and mismatch discrimination of locked nucleic acid-DNA duplexes
    Richard Owczarzy
    Department of Molecular Genetics and Biophysics, Integrated DNA Technologies, Coralville, Iowa 52241, United States
    Biochemistry 50:9352-67. 2011
    ..We hypothesize that these discrimination changes are not unique features of LNAs but originate from the shift of the duplex conformation from B-form to A-form...
  6. ncbi Melting temperatures of nucleic acids: discrepancies in analysis
    Richard Owczarzy
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA
    Biophys Chem 117:207-15. 2005
    ..New algorithm for selection of linear sloping baselines, 2nd derivative method, is suggested, which can be used to automate melting curve analysis...
  7. ncbi Effects of sodium ions on DNA duplex oligomers: improved predictions of melting temperatures
    Richard Owczarzy
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, Iowa 52241, USA
    Biochemistry 43:3537-54. 2004
    ..The results are discussed in the context of counterion condensation theory...
  8. pmc Thermodynamic treatment of oligonucleotide duplex-simplex equilibria
    Richard Owczarzy
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA
    Proc Natl Acad Sci U S A 100:14840-5. 2003
    ....
  9. ncbi Effects of fluorescent dyes, quenchers, and dangling ends on DNA duplex stability
    Bernardo G Moreira
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA
    Biochem Biophys Res Commun 327:473-84. 2005
    ..Depending on the dye/quencher combination employed, it can be important to include thermodynamic contributions from fluorophore and quencher when designing oligonucleotide probe assays...
  10. doi Predicting ultraviolet spectrum of single stranded and double stranded deoxyribonucleic acids
    Andrey V Tataurov
    Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA
    Biophys Chem 133:66-70. 2008
    ..The spectrum of any DNA oligomer can be calculated using a Microsoft Excel application that is available in the Appendix A...
  11. ncbi Studies of DNA dumbbells VIII. Melting analysis of DNA dumbbells with dinucleotide repeat stem sequences
    Kathleen E Mandell
    Department of Chemistry, University of Illinois at Chicago, 845 West Taylor Street, Room 4500, 60607, USA
    Biopolymers 82:199-221. 2006
    ....