Shao En Ong

Summary

Publications

  1. doi request reprint Unbiased identification of protein-bait interactions using biochemical enrichment and quantitative proteomics
    Shao En Ong
    The Broad Institute of MIT and Harvard, Cambridge MA 02142, USA
    Cold Spring Harb Protoc 2010:pdb.prot5400. 2010
  2. doi request reprint Identifying cellular targets of small-molecule probes and drugs with biochemical enrichment and SILAC
    Shao En Ong
    Proteomics Platform, The Broad Institute of MIT and Harvard, Cambridge, MA, USA
    Methods Mol Biol 803:129-40. 2012
  3. pmc Whole proteomes as internal standards in quantitative proteomics
    Shao En Ong
    Proteomics and Biomarker Discovery Platform, The Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
    Genome Med 2:49. 2010
  4. pmc DNA damage activates a spatially distinct late cytoplasmic cell-cycle checkpoint network controlled by MK2-mediated RNA stabilization
    H Christian Reinhardt
    David H Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02132, USA
    Mol Cell 40:34-49. 2010
  5. pmc Empirical Bayes analysis of quantitative proteomics experiments
    Adam A Margolin
    Cancer Program, The Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA
    PLoS ONE 4:e7454. 2009
  6. pmc iTRAQ labeling is superior to mTRAQ for quantitative global proteomics and phosphoproteomics
    Philipp Mertins
    Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Mol Cell Proteomics 11:M111.014423. 2012
  7. pmc Identifying the proteins to which small-molecule probes and drugs bind in cells
    Shao En Ong
    Proteomics Platform, The Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 106:4617-22. 2009
  8. pmc A mitotic phosphorylation feedback network connects Cdk1, Plk1, 53BP1, and Chk2 to inactivate the G(2)/M DNA damage checkpoint
    Marcel A T M van Vugt
    David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Biol 8:e1000287. 2010
  9. pmc AAK1 identified as an inhibitor of neuregulin-1/ErbB4-dependent neurotrophic factor signaling using integrative chemical genomics and proteomics
    Letian Kuai
    Stanley Center for Psychiatric Research, 7 Cambridge Center, Cambridge, MA 02142, USA
    Chem Biol 18:891-906. 2011
  10. doi request reprint Identification of regulators of the innate immune response to cytosolic DNA and retroviral infection by an integrative approach
    Mark N Lee
    Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA
    Nat Immunol 14:179-85. 2013

Collaborators

Detail Information

Publications15

  1. doi request reprint Unbiased identification of protein-bait interactions using biochemical enrichment and quantitative proteomics
    Shao En Ong
    The Broad Institute of MIT and Harvard, Cambridge MA 02142, USA
    Cold Spring Harb Protoc 2010:pdb.prot5400. 2010
    ..The combination of biochemical enrichment and quantitative proteomics allows rapid characterization of molecular baits with their interacting proteins, providing tremendous insight into their biological mechanisms of action...
  2. doi request reprint Identifying cellular targets of small-molecule probes and drugs with biochemical enrichment and SILAC
    Shao En Ong
    Proteomics Platform, The Broad Institute of MIT and Harvard, Cambridge, MA, USA
    Methods Mol Biol 803:129-40. 2012
    ..We describe the use of SILAC in identifying proteins that bind small-molecule probes and drugs in a cellular context...
  3. pmc Whole proteomes as internal standards in quantitative proteomics
    Shao En Ong
    Proteomics and Biomarker Discovery Platform, The Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
    Genome Med 2:49. 2010
    ....
  4. pmc DNA damage activates a spatially distinct late cytoplasmic cell-cycle checkpoint network controlled by MK2-mediated RNA stabilization
    H Christian Reinhardt
    David H Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02132, USA
    Mol Cell 40:34-49. 2010
    ..Our findings demonstrate a critical role for the MK2 pathway in the posttranscriptional regulation of gene expression as part of the DNA damage response in cancer cells...
  5. pmc Empirical Bayes analysis of quantitative proteomics experiments
    Adam A Margolin
    Cancer Program, The Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA
    PLoS ONE 4:e7454. 2009
    ....
  6. pmc iTRAQ labeling is superior to mTRAQ for quantitative global proteomics and phosphoproteomics
    Philipp Mertins
    Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Mol Cell Proteomics 11:M111.014423. 2012
    ..Spike-in experiments using peptides of defined ratios in a background of nonregulated peptides show that iTRAQ quantification is less accurate but not as variable as mTRAQ quantification...
  7. pmc Identifying the proteins to which small-molecule probes and drugs bind in cells
    Shao En Ong
    Proteomics Platform, The Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 106:4617-22. 2009
    ..Here, we describe in full detail the application of the method to identify targets of kinase inhibitors and immunophilin binders...
  8. pmc A mitotic phosphorylation feedback network connects Cdk1, Plk1, 53BP1, and Chk2 to inactivate the G(2)/M DNA damage checkpoint
    Marcel A T M van Vugt
    David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Biol 8:e1000287. 2010
    ....
  9. pmc AAK1 identified as an inhibitor of neuregulin-1/ErbB4-dependent neurotrophic factor signaling using integrative chemical genomics and proteomics
    Letian Kuai
    Stanley Center for Psychiatric Research, 7 Cambridge Center, Cambridge, MA 02142, USA
    Chem Biol 18:891-906. 2011
    ..Similar strategies should lead to the discovery of novel targets for therapeutic development...
  10. doi request reprint Identification of regulators of the innate immune response to cytosolic DNA and retroviral infection by an integrative approach
    Mark N Lee
    Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA
    Nat Immunol 14:179-85. 2013
    ....
  11. ncbi request reprint Stable isotope labeling by amino acids in cell culture for quantitative proteomics
    Shao En Ong
    Proteomics and Biomarker Discovery, The Broad Institute of MIT and Harvard, Cambridge, MA, USA
    Methods Mol Biol 359:37-52. 2007
    ..In this chapter, we provide detailed SILAC protocols and explain how to incorporate SILAC into any experiment...
  12. ncbi request reprint A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)
    Shao En Ong
    The Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Protoc 1:2650-60. 2006
    ..This procedure can be completed in 8 days...
  13. ncbi request reprint Mass spectrometry-based proteomics turns quantitative
    Shao En Ong
    The Broad Institute of MIT and Harvard, 320 Bent Street, Cambridge, Massachusetts 02141, USA
    Nat Chem Biol 1:252-62. 2005
    ..This isotopic handle facilitates direct quantification from the mass spectra. Using these quantitative approaches, precise functional information as well as temporal changes in the proteome can be captured by MS...
  14. pmc Proteomic screen defines the Polo-box domain interactome and identifies Rock2 as a Plk1 substrate
    Drew M Lowery
    Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    EMBO J 26:2262-73. 2007
    ....
  15. pmc CUB-domain-containing protein 1 (CDCP1) activates Src to promote melanoma metastasis
    Hui Liu
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 108:1379-84. 2011
    ..In addition, the Y734F mutation also eliminated enhanced Src activation. Thus, this work provides molecular mechanisms for the metastasis-enhancing functions of CDCP1...