Hans Oberleithner

Summary

Publications

  1. pmc Hydroxyfasudil-mediated inhibition of ROCK1 and ROCK2 improves kidney function in rat renal acute ischemia-reperfusion injury
    Dominik Kentrup
    Department of Internal Medicine D, Experimental Nephrology, University of Munster, Munster, Germany
    PLoS ONE 6:e26419. 2011
  2. ncbi request reprint Pathogenesis of hypertension: interactions among sodium, potassium, and aldosterone
    Eckhart Büssemaker
    Medizinische Klinik und Poliklinik D, Universitatsklinikum Munster, Munster, Germany
    Am J Kidney Dis 55:1111-20. 2010
  3. ncbi request reprint Role of rho-kinase in the regulation of vascular tone in hypertensive renal transplant recipients
    E Büssemaker
    Medizinische Klinik und Poliklinik D, Universitatsklinikum Munster, Munster, Germany
    Atherosclerosis 207:567-72. 2009
  4. ncbi request reprint New imidazole-coordinated chemotherapeutics with low epithelial toxicity
    Thomas Ludwig
    Institute of Physiology II, University of Munster, Germany
    Cell Biochem Biophys 45:31-41. 2006
  5. ncbi request reprint A pathway separate from the central channel through the nuclear pore complex for inorganic ions and small macromolecules
    Armin Kramer
    Institute of Physiology II, University of Munster, Robert Koch Strasse 27b, D 48149 Munster, Germany
    J Biol Chem 282:31437-43. 2007
  6. ncbi request reprint Nebivolol decreases endothelial cell stiffness via the estrogen receptor beta: a nano-imaging study
    Uta Hillebrand
    Department of Internal Medicine D, University of Muenster, Muenster, Germany
    J Hypertens 27:517-26. 2009
  7. doi request reprint Membrane potential depolarization decreases the stiffness of vascular endothelial cells
    Chiara Callies
    Institute of Physiology II, University of Munster, Robert Koch Str 27b, 48149 Munster, Germany
    J Cell Sci 124:1936-42. 2011
  8. doi request reprint Ménage à trois: aldosterone, sodium and nitric oxide in vascular endothelium
    Johannes Fels
    Institute of Physiology II, University of Munster, Germany
    Biochim Biophys Acta 1802:1193-202. 2010
  9. pmc Plasma sodium stiffens vascular endothelium and reduces nitric oxide release
    Hans Oberleithner
    Institute of Physiology II and Department of Internal Medicine D, University of Munster, 48149 Munster, Germany
    Proc Natl Acad Sci U S A 104:16281-6. 2007
  10. pmc Nanomechanics and sodium permeability of endothelial surface layer modulated by hawthorn extract WS 1442
    Wladimir Peters
    Institute of Physiology II, University of Munster, Munster, Germany
    PLoS ONE 7:e29972. 2012

Collaborators

Detail Information

Publications54

  1. pmc Hydroxyfasudil-mediated inhibition of ROCK1 and ROCK2 improves kidney function in rat renal acute ischemia-reperfusion injury
    Dominik Kentrup
    Department of Internal Medicine D, Experimental Nephrology, University of Munster, Munster, Germany
    PLoS ONE 6:e26419. 2011
    ..We conclude from our study that ROCK-inhibition by hydroxyfasudil significantly improves kidney function in a rat model of acute renal IRI and is therefore a potential new therapeutic option in humans...
  2. ncbi request reprint Pathogenesis of hypertension: interactions among sodium, potassium, and aldosterone
    Eckhart Büssemaker
    Medizinische Klinik und Poliklinik D, Universitatsklinikum Munster, Munster, Germany
    Am J Kidney Dis 55:1111-20. 2010
    ..A restriction in sodium intake that is accompanied by increased intake of potassium can profoundly improve the prevalence of hypertension and cardiovascular disease...
  3. ncbi request reprint Role of rho-kinase in the regulation of vascular tone in hypertensive renal transplant recipients
    E Büssemaker
    Medizinische Klinik und Poliklinik D, Universitatsklinikum Munster, Munster, Germany
    Atherosclerosis 207:567-72. 2009
    ..The greater NO-insensitive fasudil-induced increase in FBF in NTX suggests an increased ROK-mediated VSMC constrictor tone in these patients. Basal NO-mediated tone is attenuated in hypertensive NTX...
  4. ncbi request reprint New imidazole-coordinated chemotherapeutics with low epithelial toxicity
    Thomas Ludwig
    Institute of Physiology II, University of Munster, Germany
    Cell Biochem Biophys 45:31-41. 2006
    ..NICE feature the first group of platinum-based cytostatics discovered by using this system for systematic screening of new chemotherapeutics with low renal epithelial toxicity...
  5. ncbi request reprint A pathway separate from the central channel through the nuclear pore complex for inorganic ions and small macromolecules
    Armin Kramer
    Institute of Physiology II, University of Munster, Robert Koch Strasse 27b, D 48149 Munster, Germany
    J Biol Chem 282:31437-43. 2007
    ..Our data indicate a spatial separation between the active transport of macromolecules through the central channel and the passive transport of ions and small macromolecules through the pore periphery...
  6. ncbi request reprint Nebivolol decreases endothelial cell stiffness via the estrogen receptor beta: a nano-imaging study
    Uta Hillebrand
    Department of Internal Medicine D, University of Muenster, Muenster, Germany
    J Hypertens 27:517-26. 2009
    ..Nebivolol (NEB) is a [beta]1-receptor blocker with nitric oxide-dependent vasodilating properties. NEB-induced nitric oxide release is mediated through the estrogen receptor...
  7. doi request reprint Membrane potential depolarization decreases the stiffness of vascular endothelial cells
    Chiara Callies
    Institute of Physiology II, University of Munster, Robert Koch Str 27b, 48149 Munster, Germany
    J Cell Sci 124:1936-42. 2011
    ..This depolarization-induced decrease in the stiffness of endothelial cells could play a role in flow-mediated nitric-oxide-dependent vasodilation...
  8. doi request reprint Ménage à trois: aldosterone, sodium and nitric oxide in vascular endothelium
    Johannes Fels
    Institute of Physiology II, University of Munster, Germany
    Biochim Biophys Acta 1802:1193-202. 2010
    ....
  9. pmc Plasma sodium stiffens vascular endothelium and reduces nitric oxide release
    Hans Oberleithner
    Institute of Physiology II and Department of Internal Medicine D, University of Munster, 48149 Munster, Germany
    Proc Natl Acad Sci U S A 104:16281-6. 2007
    ..The results suggest that changes in plasma sodium concentration per se may affect endothelial function and thus control vascular tone...
  10. pmc Nanomechanics and sodium permeability of endothelial surface layer modulated by hawthorn extract WS 1442
    Wladimir Peters
    Institute of Physiology II, University of Munster, Munster, Germany
    PLoS ONE 7:e29972. 2012
    ..This mechanism may explain some of the vasoprotective actions of this herbal therapeutic...
  11. doi request reprint Exceptional mechanical and structural stability of HSV-1 unveiled with fluid atomic force microscopy
    Ivan Liashkovich
    Institute of Physiology II, University of Munster, Munster, Germany
    J Cell Sci 121:2287-92. 2008
    ..This stability might be the key determinant for capsid survival over long distances in the axonal cytoplasm where it is exposed to mechanical forces by molecular motors before it reaches the nuclear pore for crucial genome uncoating...
  12. doi request reprint Long-term application of the aldosterone antagonist spironolactone prevents stiff endothelial cell syndrome
    Verena Drüppel
    Institute of Physiology II, University of Munster, Munster, Germany
    FASEB J 27:3652-9. 2013
    ..This emphasizes the key role of vascular endothelium as a therapeutical target in cardiovascular disorders and might explain blood pressure independent actions of MR antagonism...
  13. ncbi request reprint Glucocorticoids remodel nuclear envelope structure and permeability
    Victor Shahin
    Institute of Physiology II, University of Munster, Robert Koch Str 27b, 48149 Munster, Germany
    J Cell Sci 118:2881-9. 2005
    ..In conclusion, the NE is a barrier of high plasticity sensitive to hydrophobic molecules. Remodelling of NE structure and permeability is a prerequisite for mediating physiological actions of glucocorticoids...
  14. ncbi request reprint Evidence for importin alpha independent nuclear translocation of glucocorticoid receptors in Xenopus laevis oocytes
    Lars Albermann
    Institute of Physiology II, University of Munster, Germany
    Cell Physiol Biochem 14:343-50. 2004
    ..This represents a restriction of nuclear import downstream of import signals...
  15. ncbi request reprint Firewall function of the endothelial glycocalyx in the regulation of sodium homeostasis
    Stefanie Korte
    Institute of Physiology II, University of Muenster, Robert Koch Strasse 27 b, 48149, Munster, Germany
    Pflugers Arch 463:269-78. 2012
    ..After eGC removal, sodium access to the system is facilitated. Thus the pump-leak system could be regulated by ambient sodium and control vascular permeability in pathophysiological conditions...
  16. ncbi request reprint Dose-dependent endothelial cell growth and stiffening by aldosterone: endothelial protection by eplerenone
    Uta Hillebrand
    Department of Internal Medicine D, University of Muenster, Germany
    J Hypertens 25:639-47. 2007
    ..Moreover, we investigated whether endothelial cells of venous and arterial origin respond similarly to aldosterone and whether the new aldosterone antagonist eplerenone effectively prevents endothelial cell growth and stiffening...
  17. ncbi request reprint Ethanol alters access to the cell nucleus
    Claudia Schafer
    Institute of Physiology II, University of Munster, Munster, Germany
    Pflugers Arch 453:809-18. 2007
    ..Within hours, the NE becomes less permeable for diffusible ions and macromolecules. This could explain altered signaling to and communication with the cell nucleus in the pathophysiology of alcohol abuse...
  18. doi request reprint Endothelial cells as vascular salt sensors
    Hans Oberleithner
    Medical Faculty, Institute of Physiology II, University of Munster, 48149 Munster, Germany
    Kidney Int 77:490-4. 2010
    ..This sensitivity may serve as a physiological feedback mechanism to regulate local blood flow. It may also have pathophysiological relevance when sodium/potassium homeostasis is disturbed...
  19. ncbi request reprint Bradykinin does not induce gap formation between human endothelial cells
    Pia Jungmann
    Institute of Physiology II, University of Munster, Robert Koch Str 27b, 48149 Munster, Germany
    Pflugers Arch 455:1007-16. 2008
    ..The combined results of both permeability and morphometric studies lead to the conclusion that bradykinin promotes transcellular fluid passage rather than increasing paracellular diffusion...
  20. doi request reprint Role of cellular mechanics in the function and life span of vascular endothelium
    Katrin Kliche
    Institute of Physiology II, University of Munster, Robert Koch Strasse 27b, Munster, Germany
    Pflugers Arch 462:209-17. 2011
    ..This may explain the recently observed beneficial effects of spironolactone on the cardiovascular system. Taken together, the review highlights the importance of elasticity for normal endothelial function...
  21. ncbi request reprint Plugs in nuclear pores: transcripts in early oocyte development identified with nanotechniques
    Andrea Schlune
    Institute of Physiology II, University of Munster, Munster, Germany
    J Cell Biochem 98:567-76. 2006
    ....
  22. ncbi request reprint Steroids dilate nuclear pores imaged with atomic force microscopy
    Victor Shahin
    Nanolab, Institute of Physiology II, University of Munster, Munster, Germany
    J Cell Physiol 202:591-601. 2005
    ..The NPC dilation signal is most likely transmitted through NPC associated filaments or yet unknown structures in the NE outer membrane. NPC dilation could have significant impact on nuclear targeting of therapeutic macromolecules...
  23. pmc Salt overload damages the glycocalyx sodium barrier of vascular endothelium
    Hans Oberleithner
    Institute of Physiology II, University of Munster, Robert Koch Str 27b, 48149, Munster, Germany
    Pflugers Arch 462:519-28. 2011
    ..Damaged eGC facilitates sodium entry into the endothelial cells. This could explain endothelial dysfunction and arterial hypertension observed in sodium abuse...
  24. pmc Translocation of aquaporin-containing vesicles to the plasma membrane is facilitated by actomyosin relaxation
    Christoph Riethmuller
    Institute of Physiology II, University of Munster, Munich, Germany
    Biophys J 94:671-8. 2008
    ..The data demonstrate that not the depolymerization of actin but a relaxation of actomyosin interaction facilitates vesicle translocation...
  25. doi request reprint Paracellular permeability of bronchial epithelium is controlled by CFTR
    Nelly Weiser
    Institute of Physiology II, University of Munster, Munster, Germany
    Cell Physiol Biochem 28:289-96. 2011
    ..Taken together, our results link CFTR dysfunction to an improper regulation of the paracellular transport route...
  26. doi request reprint Caspase-9-dependent decrease of nuclear pore channel hydrophobicity is accompanied by nuclear envelope leakiness
    Armin Kramer
    Institute of Physiology II, University of Munster, Munster, Germany
    Nanomedicine 6:605-11. 2010
    ..We conclude that caspase 9 gives rise to NE leakiness by perturbing the hydrophobicity-based barrier inside the NPC channel. This explains the high passive NE permeability in early apoptosis...
  27. ncbi request reprint The genome of HSV-1 translocates through the nuclear pore as a condensed rod-like structure
    Victor Shahin
    Institute of Physiology II, University of Munster, Robert Koch Str 27b, 48149 Munster, Germany
    J Cell Sci 119:23-30. 2006
    ..It is presumed that they are the HSV-1 genome, and that a change in NPC conformation allows translocation of this genome as a densely packaged, rodlike structure...
  28. pmc Real-time monitoring of cell elasticity reveals oscillating myosin activity
    Hermann Schillers
    Institute of Physiology II, University of Munster, Munster, Germany
    Biophys J 99:3639-46. 2010
    ..In conclusion, the collective activities of the myosin motor proteins define overall mechanical cell dynamics, reflecting specific changes of the chemical and mechanical environment...
  29. doi request reprint Nanotopography follows force in TGF-beta1 stimulated epithelium
    Gerold Thoelking
    Institute of Physiology II, University of Munster, Germany
    Nanotechnology 21:265102. 2010
    ..Since these nodular protrusions were sensitive to inhibitors of force generation, they can indicate intracellular tension. The results demonstrate a manifest impact of elevated tension on the cellular topography...
  30. pmc The soluble VEGF receptor sFlt1 contributes to endothelial dysfunction in CKD
    Giovana S Di Marco
    Department of Internal Medicine D, University Clinics Münster, Munster, Germany
    J Am Soc Nephrol 20:2235-45. 2009
    ..Our findings show that excess sFlt-1 associates with endothelial dysfunction in CKD and suggest that increased sFlt-1 may predict cardiovascular risk in CKD...
  31. doi request reprint Simultaneous mechanical stiffness and electrical potential measurements of living vascular endothelial cells using combined atomic force and epifluorescence microscopy
    Chiara Callies
    Institute of Physiology II, University of Munster, Germany
    Nanotechnology 20:175104. 2009
    ..The sustained membrane depolarization, however, is paralleled by a concomitant increase of cell stiffness. The described method is applicable for any fluorophore, which opens new perspectives in biomedical research...
  32. ncbi request reprint Nanoscale imaging and quantification of local proteolytic activity
    Stephan Kusick
    Institute of Physiology II, University of Munster, Germany
    J Cell Physiol 204:767-74. 2005
    ....
  33. doi request reprint High phosphate directly affects endothelial function by downregulating annexin II
    Giovana Seno Di Marco
    Department of Internal Medicine D, University of Munster, Munster, Germany
    Kidney Int 83:213-22. 2013
    ..In addition, high phosphate stiffened endothelial cells in vitro and in rats in vivo. Thus, our results link phosphate and adverse clinical outcomes involving the endothelium in both healthy individuals and patients with renal disease...
  34. doi request reprint Investigation of nuclear envelope structure and passive permeability
    Victor Shahin
    Institute of Physiology II, University of Munster, Munster, Germany
    Methods Mol Biol 464:161-80. 2009
    ..This chapter describes in detail the protocols for performing such investigations...
  35. doi request reprint Nuclear delivery mechanism of herpes simplex virus type 1 genome
    Ivan Liashkovich
    Institute of Physiology II, University of Munster, Robert Koch Str 27b, 48147 Münster, Germany
    J Mol Recognit 24:414-21. 2011
    ..We presume that the incoming HSV-1 capsid pinpoints the NPC by hydrophobic interactions and opens up upon binding to NPC proteins. Genome translocation is initially pressure-driven...
  36. doi request reprint Endothelial f-actin depolymerization enables leukocyte transmigration
    Laura Isac
    Institute of Physiology II, University of Munster, Germany
    Anal Bioanal Chem 399:2351-8. 2011
    ..Fluorescence microscopy confirmed a depolymerization of the f-actin strands at the invasion site. Leukocytes dramatically rearrange the endothelial cytoskeleton to form transmigratory channels...
  37. doi request reprint Sodium: a wolf in sheep's clothing
    Hans Oberleithner
    Institute of Physiology II, Medical Faculty, University Munster, Robert Koch Strasse 27b, Munster, Germany
    Blood Purif 31:82-5. 2011
    ..It becomes pathogenic, however, when the concentration of plasma sodium or potassium changes towards and beyond the upper limits of the normal range...
  38. pmc Two barriers for sodium in vascular endothelium?
    Hans Oberleithner
    Institute of Physiology II, University of Munster, Munster, Germany
    Ann Med 44:S143-8. 2012
    ..Gradually, the sodium overload damages the organism...
  39. ncbi request reprint Platinum complex cytotoxicity tested by the electrical resistance breakdown assay
    Thomas Ludwig
    Institute of Physiology II, University of Munster, Germany
    Cell Physiol Biochem 14:425-30. 2004
    ..In conclusion the electrical resistance breakdown assay provides a sensitive, continuous and cell-based assay system for the quantification of cancer cell invasiveness and evaluation of chemotherapeutics under physiological conditions...
  40. ncbi request reprint Is the vascular endothelium under the control of aldosterone? Facts and hypothesis
    Hans Oberleithner
    Institut fur Physiologie II, University of Munster, Robert Koch Strasse 27b, 48149 Munster, Germany
    Pflugers Arch 454:187-93. 2007
    ....
  41. ncbi request reprint Direct aldosterone action on mouse cardiomyocytes detected with atomic force microscopy
    Katrin Kliche
    Institute of Physiology II, University of Munster, Munster, Germany
    Cell Physiol Biochem 18:265-74. 2006
    ..These nanometer changes, detectable only with AFM in cells scanned in fluid after fixation under physiological conditions, indicate plasma membrane remodeling of cardiomyocytes by mineralocorticoids...
  42. ncbi request reprint Endothelial sodium channels trigger endothelial salt sensitivity with aging
    Moritz Paar
    From the Institute of Physiology II M P, K K V, V D, H O and Department of Internal Medicine D H P, K K, University Hospital of Munster, Munster, Germany
    Hypertension 64:391-6. 2014
    ..This mechanism might contribute to the development of age-related cardiovascular disease and suggests the usage of spironolactone and amiloride specifically in the elderly. ..
  43. pmc Nanomechanics of vascular endothelium
    Johannes Fels
    Institute of Cell Dynamics and Imaging, University of Munster, Munster, Germany
    Cell Tissue Res 355:727-37. 2014
    ..This review will focus on endothelial nanomechanics and its impact on vascular function. ..
  44. pmc Determination of erythrocyte sodium sensitivity in man
    Hans Oberleithner
    Institute of Physiology II, University of Munster, Robert Koch Str 27b, 48149, Munster, Germany
    Pflugers Arch 465:1459-66. 2013
    ..In conclusion, the SBT could possibly serve as an in vitro test system for the evaluation of erythrocyte/vascular salt sensitivity allowing follow-up measurements in the prevention and treatment of vascular dysfunctions...
  45. doi request reprint Vascular endothelium leaves fingerprints on the surface of erythrocytes
    Hans Oberleithner
    Institute of Physiology II, University of Munster, Robert Koch Str 27b, 48149, Munster, Germany
    Pflugers Arch 465:1451-8. 2013
    ..Apparently, the RBC glycocalyx reflects properties of the EC glycocalyx. This observation could have a significant impact on diagnosis and treatment of cardiovascular diseases...
  46. ncbi request reprint Vacuolar structures can be identified by AFM elasticity mapping
    Christoph Riethmuller
    Institute of Physiology II, University of Muenster, Munster, Germany
    Ultramicroscopy 107:895-901. 2007
    ..This AFM application opens up a mechanical dimension for the investigation of intracellular organelles...
  47. doi request reprint Dynamics of single potassium channel proteins in the plasma membrane of migrating cells
    Volodymyr Nechyporuk-Zloy
    Institute of Physiology II, University of Munster, Munster, Germany
    Am J Physiol Cell Physiol 294:C1096-102. 2008
    ..Thus tracking fluorescent particles within the glycocalyx opens up a possibility to characterize the pericellular nanoenvironment...
  48. ncbi request reprint Nephrotoxicity of platinum complexes is related to basolateral organic cation transport
    Thomas Ludwig
    Institut fur Physiologie II, Westfalische Wilhelms Universitat Munster, Munster, Germany
    Kidney Int 66:196-202. 2004
    ..By means of this assay system we addressed the question whether the side of application of renal epithelia influences platinum complex toxicity...
  49. ncbi request reprint The role of ENaC in vascular endothelium
    Kristina Kusche-Vihrog
    Institute of Physiology II, University of Munster, Robert Koch Str 27b, 48149, Munster, Germany
    Pflugers Arch 466:851-9. 2014
    ....
  50. ncbi request reprint Unorthodox sites and modes of aldosterone action
    Hans Oberleithner
    Nanolab, Institute of Physiology II, University of Munster, 48149 Muenster, Germany
    News Physiol Sci 19:51-4. 2004
    ..Endothelial cells gain sensitivity to diuretics when exposed to aldosterone. Atomic force microscopy detects such phenomena. It is speculated that endothelium and kidney join forces in the regulation of body fluids...
  51. ncbi request reprint Shape and volume of living aldosterone-sensitive cells imaged with the atomic force microscope
    Stefan W Schneider
    Department of Dermatology, Westfalische Wilhelms Universitat, Munster, Germany
    Methods Mol Biol 242:255-79. 2004
  52. ncbi request reprint Imaging CFTR: a tail to tail dimer with a central pore
    Hermann Schillers
    Institute of Physiology II, Nanolab, University of Munster, Germany
    Cell Physiol Biochem 14:1-10. 2004
    ..This molecular arrangement could represent the CFTR chloride channel configuration, operative in native plasma membrane...
  53. ncbi request reprint Fixed endothelial cells exhibit physiologically relevant nanomechanics of the cortical actin web
    Kai Bodo Grimm
    Institute of Physiology II, University of Munster, Robert Koch Str 27b, 48149 Munster, Germany
    Nanotechnology 25:215101. 2014
    ..We conclude that fixation interferes with motor proteins while maintaining a functional cortical actin web. Thus, fixation of cells opens up the prospect of differentially studying the actions of cellular myosin and actin. ..
  54. ncbi request reprint IADS, a decomposition product of DIDS activates a cation conductance in Xenopus oocytes and human erythrocytes: new compound for the diagnosis of cystic fibrosis
    Astrid Stumpf
    Institute of Physiology II, University of Munster, Munster, Germany
    Cell Physiol Biochem 18:243-52. 2006
    ..Thus, IADS stimulated activation of a cation conductance could form the basis for a novel diagnostic test of cystic fibrosis...