Davide Serruto

Summary

Affiliation: Novartis Institutes for BioMedical Research

Publications

  1. pmc Transcriptome analysis of Neisseria meningitidis in human whole blood and mutagenesis studies identify virulence factors involved in blood survival
    Hebert Echenique-Rivera
    Novartis Vaccines and Diagnostics, Siena, Italy
    PLoS Pathog 7:e1002027. 2011
  2. pmc The new multicomponent vaccine against meningococcal serogroup B, 4CMenB: immunological, functional and structural characterization of the antigens
    Davide Serruto
    Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy
    Vaccine 30:B87-97. 2012
  3. pmc Neisseria meningitidis NalP cleaves human complement C3, facilitating degradation of C3b and survival in human serum
    Elena Del Tordello
    Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy
    Proc Natl Acad Sci U S A 111:427-32. 2014
  4. pmc Neisseria meningitidis GNA2132, a heparin-binding protein that induces protective immunity in humans
    Davide Serruto
    Novartis Vaccines and Diagnostics, 53100 Siena, Italy
    Proc Natl Acad Sci U S A 107:3770-5. 2010
  5. doi request reprint Influence of sequence variability on bactericidal activity sera induced by Factor H binding protein variant 1.1
    Brunella Brunelli
    Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy
    Vaccine 29:1072-81. 2011
  6. ncbi request reprint Neisseria meningitidis NhhA is a multifunctional trimeric autotransporter adhesin
    Maria Scarselli
    Novartis Vaccines, Via Fiorentina 1, 53100 Siena, Italy
    Mol Microbiol 61:631-44. 2006
  7. ncbi request reprint Biotechnology and vaccines: application of functional genomics to Neisseria meningitidis and other bacterial pathogens
    Davide Serruto
    IRIS, Chiron Vaccines, Via Fiorentina 1, 53100 Siena, Italy
    J Biotechnol 113:15-32. 2004
  8. doi request reprint HadA is an atypical new multifunctional trimeric coiled-coil adhesin of Haemophilus influenzae biogroup aegyptius, which promotes entry into host cells
    Davide Serruto
    Research Center, Novartis Vaccines and Diagnostics, Siena, Italy
    Cell Microbiol 11:1044-63. 2009
  9. pmc A naturally occurring single-residue mutation in the translocator domain of Neisseria meningitidis NhhA affects trimerization, surface localization, and adhesive capabilities
    Hebert Echenique-Rivera
    Novartis Vaccines and Diagnostics, Via Fiorentina, 1, 53100 Siena, Italy
    Infect Immun 79:4308-21. 2011
  10. ncbi request reprint Measuring antigen-specific bactericidal responses to a multicomponent vaccine against serogroup B meningococcus
    Marzia M Giuliani
    Novartis Vaccines and Diagnostics, Via Fiorentina 1, Siena 53100, Italy
    Vaccine 28:5023-30. 2010

Collaborators

Detail Information

Publications33

  1. pmc Transcriptome analysis of Neisseria meningitidis in human whole blood and mutagenesis studies identify virulence factors involved in blood survival
    Hebert Echenique-Rivera
    Novartis Vaccines and Diagnostics, Siena, Italy
    PLoS Pathog 7:e1002027. 2011
    ..This increased knowledge of how Nm responds to adaptation in blood could also be helpful to develop diagnostic and therapeutic strategies to control the devastating disease cause by this microorganism...
  2. pmc The new multicomponent vaccine against meningococcal serogroup B, 4CMenB: immunological, functional and structural characterization of the antigens
    Davide Serruto
    Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy
    Vaccine 30:B87-97. 2012
    ..This review describes the successful story of the development of the 4CMenB vaccine, with particular emphasis on the functional, immunological and structural characterization of the protein antigens included in the vaccine...
  3. pmc Neisseria meningitidis NalP cleaves human complement C3, facilitating degradation of C3b and survival in human serum
    Elena Del Tordello
    Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy
    Proc Natl Acad Sci U S A 111:427-32. 2014
    ..We conclude that NalP is an important factor to increase the survival of Nm in human serum. ..
  4. pmc Neisseria meningitidis GNA2132, a heparin-binding protein that induces protective immunity in humans
    Davide Serruto
    Novartis Vaccines and Diagnostics, 53100 Siena, Italy
    Proc Natl Acad Sci U S A 107:3770-5. 2010
    ..We conclude that GNA2132 is an important protective antigen of N. meningitidis and we propose to rename it, Neisserial Heparin Binding Antigen (NHBA)...
  5. doi request reprint Influence of sequence variability on bactericidal activity sera induced by Factor H binding protein variant 1.1
    Brunella Brunelli
    Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy
    Vaccine 29:1072-81. 2011
    ....
  6. ncbi request reprint Neisseria meningitidis NhhA is a multifunctional trimeric autotransporter adhesin
    Maria Scarselli
    Novartis Vaccines, Via Fiorentina 1, 53100 Siena, Italy
    Mol Microbiol 61:631-44. 2006
    ..Collectively, our results underline a putative role of NhhA in meningococcal pathogenesis and ascertain its structural and functional belonging to the emerging group of bacterial autotransporter adhesins with trimeric architecture...
  7. ncbi request reprint Biotechnology and vaccines: application of functional genomics to Neisseria meningitidis and other bacterial pathogens
    Davide Serruto
    IRIS, Chiron Vaccines, Via Fiorentina 1, 53100 Siena, Italy
    J Biotechnol 113:15-32. 2004
    ..In addition, we describe the potential of these powerful technologies in understanding the pathogenesis of various bacteria...
  8. doi request reprint HadA is an atypical new multifunctional trimeric coiled-coil adhesin of Haemophilus influenzae biogroup aegyptius, which promotes entry into host cells
    Davide Serruto
    Research Center, Novartis Vaccines and Diagnostics, Siena, Italy
    Cell Microbiol 11:1044-63. 2009
    ..These data give new insights on the structure-function relationship of oligomeric coiled-coil adhesins and suggest a potential role of this protein in the pathogenesis of BPF...
  9. pmc A naturally occurring single-residue mutation in the translocator domain of Neisseria meningitidis NhhA affects trimerization, surface localization, and adhesive capabilities
    Hebert Echenique-Rivera
    Novartis Vaccines and Diagnostics, Via Fiorentina, 1, 53100 Siena, Italy
    Infect Immun 79:4308-21. 2011
    ..Also, they might have important implications for the evaluation of NhhA as a vaccine candidate...
  10. ncbi request reprint Measuring antigen-specific bactericidal responses to a multicomponent vaccine against serogroup B meningococcus
    Marzia M Giuliani
    Novartis Vaccines and Diagnostics, Via Fiorentina 1, Siena 53100, Italy
    Vaccine 28:5023-30. 2010
    ....
  11. pmc A universal vaccine for serogroup B meningococcus
    Marzia M Giuliani
    Novartis Vaccines, Via Fiorentina 1, 53100 Siena, Italy
    Proc Natl Acad Sci U S A 103:10834-9. 2006
    ..The strain coverage could be increased to 90% and above by the addition of CpG oligonucleotides or by using MF59 as adjuvant. The vaccine has the potential to conquer one of the most devastating diseases of childhood...
  12. pmc The Hfq-dependent small noncoding RNA NrrF directly mediates Fur-dependent positive regulation of succinate dehydrogenase in Neisseria meningitidis
    Matteo M E Metruccio
    Novartis Vaccines, Via Fiorentina 1, 53100 Siena, Italy
    J Bacteriol 191:1330-42. 2009
    ..In addition, while the upregulation of sodB and fumB by Fur is dependent on the Hfq protein, it is unaffected in the nrrF knockout, which suggests that there is more than one sRNA regulator involved in iron homeostasis in meningococcus...
  13. doi request reprint Genome-based approaches to develop vaccines against bacterial pathogens
    Davide Serruto
    Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy
    Vaccine 27:3245-50. 2009
    ..This review describes the applications of genome-based approaches in the development of new vaccines against bacterial pathogens...
  14. pmc Qualitative and quantitative assessment of meningococcal antigens to evaluate the potential strain coverage of protein-based vaccines
    John Donnelly
    Novartis Vaccines and Diagnostics, 53100 Siena, Italy
    Proc Natl Acad Sci U S A 107:19490-5. 2010
    ..This assay allows typing of large panels of strains and prediction of coverage of protein-based meningococcal vaccines. Similar assays may be used for protein-based vaccines against other bacteria...
  15. doi request reprint A novel Hfq-dependent sRNA that is under FNR control and is synthesized in oxygen limitation in Neisseria meningitidis
    Laura Fantappiè
    Novartis Vaccines, Microbial Molecular Biology, Via Fiorentina 1, 53100 Siena, Italy
    Mol Microbiol 80:507-23. 2011
    ..Furthermore the presence of similar conserved regulatory sequences in all Neisseria spp. to date suggests that an analogous FNR-regulated sRNA, with a variable 5' sequence, may be ubiquitous to all commensals and pathogens of the Genus...
  16. ncbi request reprint The genome revolution in vaccine research
    Barbara Capecchi
    IRIS, Chiron s r l, Via Fiorentina 1, 53100 Siena, Italy
    Curr Issues Mol Biol 6:17-27. 2004
    ..The concept of "Reverse Vaccinology" can be easily applied to all the pathogens for which vaccines are not yet available and can be extended to parasites and viruses...
  17. pmc Neisseria meningitidis is structured in clades associated with restriction modification systems that modulate homologous recombination
    Sonia Budroni
    Research Center, Novartis Vaccines and Diagnostics, 53100 Siena, Italy
    Proc Natl Acad Sci U S A 108:4494-9. 2011
    ....
  18. doi request reprint Functional genomics studies of the human pathogen Neisseria meningitidis
    Elena Del Tordello
    Novartis Vaccines and Diagnostics, Siena, Italy
    Brief Funct Genomics 12:328-40. 2013
    ..meningitidis. ..
  19. pmc Analysis of the regulated transcriptome of Neisseria meningitidis in human blood using a tiling array
    Elena Del Tordello
    Novartis Vaccines and Diagnostics, Siena, Italy
    J Bacteriol 194:6217-32. 2012
    ..meningitidis, extensive antisense transcription activity. This analysis suggests the presence of a circuit of regulatory RNA elements used by N. meningitidis to adapt to proliferate in human blood that is worthy of further investigation...
  20. ncbi request reprint Post-genomic vaccine development
    Davide Serruto
    Novartis Vaccines, Via Fiorentina, 53100 Siena, Italy
    FEBS Lett 580:2985-92. 2006
    ..In the post-genomic era, the next challenge of the vaccine biologist will be the merging of the vaccinology with structural biology...
  21. doi request reprint Molecular mechanisms of complement evasion: learning from staphylococci and meningococci
    Davide Serruto
    Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy
    Nat Rev Microbiol 8:393-9. 2010
    ..This information is fundamental to our understanding of bacterial pathogenesis and may facilitate the design of better vaccines...
  22. ncbi request reprint Staphylococcus aureus FhuD2 is involved in the early phase of staphylococcal dissemination and generates protective immunity in mice
    Ravi P N Mishra
    Novartis Vaccines, Research Center, Siena, Italy
    J Infect Dis 206:1041-9. 2012
    ..Herein we show that the staphylococcal iron-hydroxamate uptake system is important in invasive infection and functions as an efficacious vaccine target...
  23. ncbi request reprint Neisseria meningitidis App, a new adhesin with autocatalytic serine protease activity
    Davide Serruto
    IRIS, Chiron s r l, Via Fiorentina 1, 53100 Siena, Italy
    Mol Microbiol 48:323-34. 2003
    ..The serine protease activity is localized at the amino-terminal domain, whereas the binding domain is in the carboxy-terminal region. The role of App in adhesion was confirmed also in N. meningitidis...
  24. pmc Identification of a novel zinc metalloprotease through a global analysis of Clostridium difficile extracellular proteins
    Valeria Cafardi
    Novartis Vaccines and Diagnostics, Siena, Italy
    PLoS ONE 8:e81306. 2013
    ..Thus, by analyzing the exoproteome of C. difficile, we identified a novel extracellular metalloprotease that may be important in key steps of clostridial pathogenesis. ..
  25. ncbi request reprint NarE: a novel ADP-ribosyltransferase from Neisseria meningitidis
    Vega Masignani
    IRIS, Chiron s r l, Via Fiorentina 1, 53100 Siena, Italy
    Mol Microbiol 50:1055-67. 2003
    ..When present, the gene is 100% conserved in sequence and is inserted upstream of and co-transcribed with the lipoamide dehydrogenase E3 gene. Possible roles in the pathogenesis of N. meningitidis are discussed...
  26. pmc Multiple factors modulate biofilm formation by the anaerobic pathogen Clostridium difficile
    Tanja Ðapa
    Novartis Vaccines and Diagnostics, Siena, Italy
    J Bacteriol 195:545-55. 2013
    ..Our data suggest that biofilm formation by C. difficile is a complex multifactorial process and may be a crucial mechanism for clostridial persistence in the host...
  27. pmc Vaccination against Neisseria meningitidis using three variants of the lipoprotein GNA1870
    Vega Masignani
    IRIS, Chiron Srl, Via Fiorentina 1, 53100 Siena, Italy
    J Exp Med 197:789-99. 2003
    ..The novel antigen is a top candidate for the development of a new vaccine against meningococcus...
  28. ncbi request reprint Two years into reverse vaccinology
    Jeannette Adu-Bobie
    IRIS, Chiron SpA, Via Fiorentina 1, 53100, Siena, Italy
    Vaccine 21:605-10. 2003
    ..The Neisseria meningitidis serogroup B project, the first example of Reverse Vaccinology, as well as the application of this strategy to develop novel vaccines against other human pathogens are discussed...
  29. pmc Streptococcus pyogenes SpyCEP influences host-pathogen interactions during infection in a murine air pouch model
    Nico Chiappini
    Research Centre, Novartis Vaccines and Diagnostics, Siena, Italy
    PLoS ONE 7:e40411. 2012
    ..The animal model we employed shows promise for broad application in the study of bacterial pathogenesis...
  30. doi request reprint Recombinant bacterial vaccines
    Meera Unnikrishnan
    Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100, Siena, Italy
    Curr Opin Immunol 24:337-42. 2012
    ..Novel approaches in recombinant DNA technology, genomics and structural biology have revolutionized the way vaccine candidates are developed and will make a significant impact in the generation of safer and more effective vaccines...
  31. ncbi request reprint The signal peptide sequence of a lytic transglycosylase of Neisseria meningitidis is involved in regulation of gene expression
    Davide Serruto
    IRIS, Chiron s r l, Via Fiorentina 1, 53100 Siena, Italy
    Microbiology 150:1427-37. 2004
    ....
  32. doi request reprint Microbiology in the post-genomic era
    Duccio Medini
    Novartis Vaccines and Diagnostics, 53100 Siena, Italy
    Nat Rev Microbiol 6:419-30. 2008
    ....
  33. doi request reprint Future challenges in the elimination of bacterial meningitis
    Matthew J Bottomley
    Novartis Vaccines and Diagnostics Srl, Via Fiorentina 1, 53100 Siena, Italy
    Vaccine 30:B78-86. 2012
    ..We discuss how improved pathogen surveillance, implementation of current vaccines, and development of novel vaccines may be expected to further reduce bacterial meningitis and related diseases in the future...