Lorenz M Mayr

Summary

Affiliation: Novartis Institutes for BioMedical Research

Publications

  1. ncbi request reprint Structural analysis of the mitotic regulator hPin1 in solution: insights into domain architecture and substrate binding
    Elena Bayer
    Max Planck Institute for Molecular Physiology, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    J Biol Chem 278:26183-93. 2003
  2. ncbi request reprint SpeedScreen: The "missing link" between genomics and lead discovery
    Hartmut Zehender
    Novartis Institutes for BioMedical Research, Discovery Technologies Basel Screening Operations, 4002 Basel, Switzerland
    J Biomol Screen 9:498-505. 2004
  3. ncbi request reprint Development and implementation of a highly miniaturized confocal 2D-FIDA-based high-throughput screening assay to search for active site modulators of the human heat shock protein 90beta
    Alain Schilb
    Novartis Institute for Biomolecular Research, Discovery Technologies, Basel, Switzerland
    J Biomol Screen 9:569-77. 2004
  4. doi request reprint The future of high-throughput screening
    Lorenz M Mayr
    Novartis Institutes of Biomedical Research, Center of Proteomic Chemistry, Basel, Switzerland
    J Biomol Screen 13:443-8. 2008
  5. doi request reprint Novel trends in high-throughput screening
    Lorenz M Mayr
    Novartis Institutes for BioMedical Research, Center of Proteomic Chemistry, Protease Platform, Novartis Campus, CH 4002 Basel, Switzerland
    Curr Opin Pharmacol 9:580-8. 2009
  6. pmc Single fluorescent protein-based Ca2+ sensors with increased dynamic range
    Ekaterina A Souslova
    Shemiakin Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho Maklaya 16 10, Moscow, Russia
    BMC Biotechnol 7:37. 2007
  7. ncbi request reprint Time reduction and process optimization of the baculovirus expression system for more efficient recombinant protein production in insect cells
    Bjorn Philipps
    Discovery Technologies, Novartis Institutes for BioMedical Research, Basel, Switzerland
    Protein Expr Purif 42:211-8. 2005
  8. doi request reprint Development of a novel Gateway-based vector system for efficient, multiparallel protein expression in Escherichia coli
    Felix Freuler
    Novartis Institutes for Biomedical Research NIBR, Center for Proteomic Chemistry CPC, Forum 1, Novartis Campus, CH 4056 Basel, Switzerland
    Protein Expr Purif 59:232-41. 2008
  9. ncbi request reprint Application of mass spectrometry technologies for the discovery of low-molecular weight modulators of enzymes and protein-protein interactions
    Hartmut Zehender
    Novartis Institutes for Biomedical Research NIBR, WSJ 350 1 14, 4002 Basel, Switzerland
    Curr Opin Chem Biol 11:511-7. 2007
  10. ncbi request reprint New methods for efficient protein production in drug discovery
    Lukas Leder
    Novartis Institutes for BioMedical Research, Center for Proteomic Chemistry, WSJ 88 6 02A, Lichtstrasse 35, CH 4056 Basel, Switzerland
    Curr Opin Drug Discov Devel 10:193-202. 2007

Collaborators

Detail Information

Publications19

  1. ncbi request reprint Structural analysis of the mitotic regulator hPin1 in solution: insights into domain architecture and substrate binding
    Elena Bayer
    Max Planck Institute for Molecular Physiology, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    J Biol Chem 278:26183-93. 2003
    ..In the absence of sulfate and/or reducing agent this residue seems to promote aggregation, as observed in hPin1 solutions in vitro...
  2. ncbi request reprint SpeedScreen: The "missing link" between genomics and lead discovery
    Hartmut Zehender
    Novartis Institutes for BioMedical Research, Discovery Technologies Basel Screening Operations, 4002 Basel, Switzerland
    J Biomol Screen 9:498-505. 2004
    ..Advantages and limitations of SpeedScreen compared to alternative screening technologies are discussed, and an example is given from a SpeedScreen campaign applying this innovative affinity selection concept in HTS...
  3. ncbi request reprint Development and implementation of a highly miniaturized confocal 2D-FIDA-based high-throughput screening assay to search for active site modulators of the human heat shock protein 90beta
    Alain Schilb
    Novartis Institute for Biomolecular Research, Discovery Technologies, Basel, Switzerland
    J Biomol Screen 9:569-77. 2004
    ..The assay is used to identify small molecular weight compounds displacing TAMRA-radicicol. Such compounds are believed to be important molecules in the discovery of novel anticancer drugs...
  4. doi request reprint The future of high-throughput screening
    Lorenz M Mayr
    Novartis Institutes of Biomedical Research, Center of Proteomic Chemistry, Basel, Switzerland
    J Biomol Screen 13:443-8. 2008
    ..The authors predict that, ultimately, each hit-finding strategy will be much more project related, tailor-made, and better integrated into the broader drug discovery efforts...
  5. doi request reprint Novel trends in high-throughput screening
    Lorenz M Mayr
    Novartis Institutes for BioMedical Research, Center of Proteomic Chemistry, Protease Platform, Novartis Campus, CH 4002 Basel, Switzerland
    Curr Opin Pharmacol 9:580-8. 2009
    ..We predict that, ultimately, each hit finding strategy will be much more project-related, tailor-made, and better integrated into the broader drug discovery efforts...
  6. pmc Single fluorescent protein-based Ca2+ sensors with increased dynamic range
    Ekaterina A Souslova
    Shemiakin Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho Maklaya 16 10, Moscow, Russia
    BMC Biotechnol 7:37. 2007
    ....
  7. ncbi request reprint Time reduction and process optimization of the baculovirus expression system for more efficient recombinant protein production in insect cells
    Bjorn Philipps
    Discovery Technologies, Novartis Institutes for BioMedical Research, Basel, Switzerland
    Protein Expr Purif 42:211-8. 2005
    ..This new expression system is a significant step forward towards industrialized protein production in both, industry and academia...
  8. doi request reprint Development of a novel Gateway-based vector system for efficient, multiparallel protein expression in Escherichia coli
    Felix Freuler
    Novartis Institutes for Biomedical Research NIBR, Center for Proteomic Chemistry CPC, Forum 1, Novartis Campus, CH 4056 Basel, Switzerland
    Protein Expr Purif 59:232-41. 2008
    ..In summary, this system has some benefits over the widely used and commercially available Gateway standard system, and it enables many different combinations for expression constructs from a single gene of interest...
  9. ncbi request reprint Application of mass spectrometry technologies for the discovery of low-molecular weight modulators of enzymes and protein-protein interactions
    Hartmut Zehender
    Novartis Institutes for Biomedical Research NIBR, WSJ 350 1 14, 4002 Basel, Switzerland
    Curr Opin Chem Biol 11:511-7. 2007
    ....
  10. ncbi request reprint New methods for efficient protein production in drug discovery
    Lukas Leder
    Novartis Institutes for BioMedical Research, Center for Proteomic Chemistry, WSJ 88 6 02A, Lichtstrasse 35, CH 4056 Basel, Switzerland
    Curr Opin Drug Discov Devel 10:193-202. 2007
    ..In addition, several techniques allow the attachment of small molecular labels to proteins in a site-specific manner, which can be highly useful for various important experimental techniques in current drug discovery...
  11. ncbi request reprint Optimization of protein expression systems for modern drug discovery
    Michael Forstner
    Protein Expression and Purification Novartis Institutes of BioMedical Research, Discovery Technologies Lead Discovery Center CH 4002 Basel, Switzerland
    Expert Rev Proteomics 4:67-78. 2007
    ..In addition, the big challenges of recombinant overexpression of membrane and secreted proteins are tackled, and some new methods are reviewed...
  12. ncbi request reprint Readout technologies for highly miniaturized kinase assays applicable to high-throughput screening in a 1536-well format
    Martin Klumpp
    Novartis Institute for Biomedical Research, Basel, Switzerland
    J Biomol Screen 11:617-33. 2006
    ..Therefore, the target-specific selection of the most appropriate readout technology is recommended to ensure maximal relevance of HTS campaigns...
  13. ncbi request reprint A chemoinformatics analysis of hit lists obtained from high-throughput affinity-selection screening
    Nathan Brown
    Novartis Institutes for BioMedical Research, Basel, Switzerland
    J Biomol Screen 11:123-30. 2006
    ....
  14. ncbi request reprint Baculovirus expression system for magnetic sorting of infected cells and enhanced titer determination
    Bjorn Philipps
    Novartis Pharma AG, Lead Discovery Center LDC, CH 4002 Basel, Switzerland
    Biotechniques 36:80-3. 2004
    ....
  15. ncbi request reprint A baculovirus expression vector system for simultaneous protein expression in insect and mammalian cells
    Bjorn Philipps
    Discovery Technologies, Novartis Institutes for BioMedical Research, CH 4002 Basel, Switzerland
    Biotechnol Prog 21:708-11. 2005
    ..This reduces time and effort for finding appropriate expression conditions in various hosts...
  16. doi request reprint Knowledge-based virtual screening: application to the MDM4/p53 protein-protein interaction
    Edgar Jacoby
    Novartis Institutes for BioMedical Research, Novartis Pharma AG, Basel, Switzerland
    Methods Mol Biol 575:173-94. 2009
    ..Novel, selective and dual hits are discovered for both systems. A hit rate analysis will be provided compared to the full HTS (High-throughput Screening)...
  17. ncbi request reprint Proteinase-activated receptors (PARs)--the PAR3 Neo-N-terminal peptide TFRGAP interacts with PAR1
    Roland Kaufmann
    Department of General and Visceral Surgery, Research Center Lobeda, Medical Faculty at the Friedrich Schiller University Jena, Erlanger Allee 101, 07747 Jena, Germany
    Regul Pept 125:61-6. 2005
    ..These studies provide evidence that analogues of the PAR3 tethered ligand can mediate cell signaling by interaction with PAR1-type thrombin receptors...
  18. ncbi request reprint A new high affinity binding site for suppressor of cytokine signaling-3 on the erythropoietin receptor
    Michael Hortner
    Bayer Pharma Research Center, Wuppertal, Germany
    Eur J Biochem 269:2516-26. 2002
    ....
  19. ncbi request reprint Solution structure of human GABA(A) receptor-associated protein GABARAP: implications for biolgoical funcrion and its regulation
    Thomas Stangler
    Institut fur Molekulare Biotechnologie, Beutenbergstr 11, 07745 Jena, Germany
    J Biol Chem 277:13363-6. 2002
    ..The possible biological relevance of these observations for the regulation of GABARAP interactions and functions is discussed...