Siew Pheng Lim

Summary

Affiliation: Novartis Institutes for BioMedical Research

Publications

  1. doi request reprint A scintillation proximity assay for dengue virus NS5 2'-O-methyltransferase-kinetic and inhibition analyses
    Siew Pheng Lim
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore siew
    Antiviral Res 80:360-9. 2008
  2. ncbi request reprint Peptide inhibitors of dengue virus NS3 protease. Part 2: SAR study of tetrapeptide aldehyde inhibitors
    Zheng Yin
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, 138670 Singapore
    Bioorg Med Chem Lett 16:40-3. 2006
  3. ncbi request reprint Yellow fever virus NS3 protease: peptide-inhibition studies
    Kristina Löhr
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670
    J Gen Virol 88:2223-7. 2007
  4. ncbi request reprint Peptide inhibitors of West Nile NS3 protease: SAR study of tetrapeptide aldehyde inhibitors
    John E Knox
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, 138670 Singapore
    J Med Chem 49:6585-90. 2006
  5. doi request reprint Higher catalytic efficiency of N-7-methylation is responsible for processive N-7 and 2'-O methyltransferase activity in dengue virus
    Ka Yan Chung
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, 138670, Singapore
    Virology 402:52-60. 2010
  6. doi request reprint Biochemical and genetic characterization of dengue virus methyltransferase
    Hongping Dong
    Novartis Institute for Tropical Diseases, Chromos, Singapore 138670, Singapore
    Virology 405:568-78. 2010
  7. pmc Small molecule inhibitors that selectively block dengue virus methyltransferase
    Siew Pheng Lim
    Novartis Institute for Tropical Diseases, 05 01 Chromos, Singapore
    J Biol Chem 286:6233-40. 2011
  8. ncbi request reprint Finding new medicines for flaviviral targets
    Thomas H Keller
    Novartis Institute for Tropical Diseases NITD, 10 Biopolis Road, 05 01 Chromos, 138670 Singapore
    Novartis Found Symp 277:102-14; discussion 114-9, 251-3. 2006
  9. doi request reprint Ten years of dengue drug discovery: progress and prospects
    Siew Pheng Lim
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore
    Antiviral Res 100:500-19. 2013
  10. doi request reprint Strategies for development of Dengue virus inhibitors
    Christian G Noble
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore
    Antiviral Res 85:450-62. 2010

Collaborators

Detail Information

Publications12

  1. doi request reprint A scintillation proximity assay for dengue virus NS5 2'-O-methyltransferase-kinetic and inhibition analyses
    Siew Pheng Lim
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore siew
    Antiviral Res 80:360-9. 2008
    ..It is suitable for determination of enzymatic activities of a wide variety of RNA capping MTases...
  2. ncbi request reprint Peptide inhibitors of dengue virus NS3 protease. Part 2: SAR study of tetrapeptide aldehyde inhibitors
    Zheng Yin
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, 138670 Singapore
    Bioorg Med Chem Lett 16:40-3. 2006
    ..Tripeptide and dipeptide aldehyde inhibitors also show low micromolar activity. Additionally, an effective non-basic, uncharged replacement of P(1) Arg is identified...
  3. ncbi request reprint Yellow fever virus NS3 protease: peptide-inhibition studies
    Kristina Löhr
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670
    J Gen Virol 88:2223-7. 2007
    ..These findings indicate that the characteristics of YFV protease are very similar to those reported for dengue and West Nile virus proteases, and suggest that pan-flavivirus NS3 protease drugs may be developed for flaviviral diseases...
  4. ncbi request reprint Peptide inhibitors of West Nile NS3 protease: SAR study of tetrapeptide aldehyde inhibitors
    John E Knox
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, 138670 Singapore
    J Med Chem 49:6585-90. 2006
    ....
  5. doi request reprint Higher catalytic efficiency of N-7-methylation is responsible for processive N-7 and 2'-O methyltransferase activity in dengue virus
    Ka Yan Chung
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, 138670, Singapore
    Virology 402:52-60. 2010
    ..In the context of long viral RNA sequences, and compared to S-adenosyl-homocysteine, sinefungin was about 60- and 12-folds more potent against dengue N-7 and 2'-O MTase activity, exhibiting IC(50) values of 30 and 41nM, respectively...
  6. doi request reprint Biochemical and genetic characterization of dengue virus methyltransferase
    Hongping Dong
    Novartis Institute for Tropical Diseases, Chromos, Singapore 138670, Singapore
    Virology 405:568-78. 2010
    ....
  7. pmc Small molecule inhibitors that selectively block dengue virus methyltransferase
    Siew Pheng Lim
    Novartis Institute for Tropical Diseases, 05 01 Chromos, Singapore
    J Biol Chem 286:6233-40. 2011
    ..These findings demonstrate that one of the major hurdles for the development of methyltransferase-based therapeutics, namely selectivity for disease-related methyltransferases, can be overcome...
  8. ncbi request reprint Finding new medicines for flaviviral targets
    Thomas H Keller
    Novartis Institute for Tropical Diseases NITD, 10 Biopolis Road, 05 01 Chromos, 138670 Singapore
    Novartis Found Symp 277:102-14; discussion 114-9, 251-3. 2006
    ..NS3 helicase can also be considered a viable drug target for flaviviral diseases. It has however proved to be a challenging for HCV and selectivity issues versus human helicases must be overcome...
  9. doi request reprint Ten years of dengue drug discovery: progress and prospects
    Siew Pheng Lim
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore
    Antiviral Res 100:500-19. 2013
    ..The knowledge accumulated during the past decade has provided a better rationale for ongoing dengue drug discovery. Though challenging, we are optimistic that this continuous, concerted effort will lead to an effective dengue therapy. ..
  10. doi request reprint Strategies for development of Dengue virus inhibitors
    Christian G Noble
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore
    Antiviral Res 85:450-62. 2010
    ..The strategies discussed in this report should be applicable to antiviral development of other viruses...
  11. ncbi request reprint Peptide inhibitors of Dengue virus NS3 protease. Part 1: Warhead
    Zheng Yin
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore
    Bioorg Med Chem Lett 16:36-9. 2006
    ..Effective inhibition was achieved by peptide inhibitors with electrophilic warheads such as aldehyde, trifluoromethyl ketone, and boronic acid. A boronic acid has the highest affinity, exhibiting a K(i) of 43 nM...
  12. doi request reprint West Nile virus drug discovery
    Siew Pheng Lim
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, Chromos 05 01, Singapore 138670, Singapore
    Viruses 5:2977-3006. 2013
    ..The knowledge accumulated during the past fifteen years has provided better rationale for the ongoing WNV and other flavivirus antiviral development. ..