Siew Pheng Lim

Summary

Affiliation: Novartis Institutes for BioMedical Research

Publications

  1. ncbi A scintillation proximity assay for dengue virus NS5 2'-O-methyltransferase-kinetic and inhibition analyses
    Siew Pheng Lim
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore siew
    Antiviral Res 80:360-9. 2008
  2. ncbi Peptide inhibitors of dengue virus NS3 protease. Part 2: SAR study of tetrapeptide aldehyde inhibitors
    Zheng Yin
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, 138670 Singapore
    Bioorg Med Chem Lett 16:40-3. 2006
  3. ncbi Yellow fever virus NS3 protease: peptide-inhibition studies
    Kristina Löhr
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670
    J Gen Virol 88:2223-7. 2007
  4. ncbi Higher catalytic efficiency of N-7-methylation is responsible for processive N-7 and 2'-O methyltransferase activity in dengue virus
    Ka Yan Chung
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, 138670, Singapore
    Virology 402:52-60. 2010
  5. ncbi Peptide inhibitors of West Nile NS3 protease: SAR study of tetrapeptide aldehyde inhibitors
    John E Knox
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01 Chromos, 138670 Singapore
    J Med Chem 49:6585-90. 2006
  6. ncbi Small molecule inhibitors that selectively block dengue virus methyltransferase
    Siew Pheng Lim
    Novartis Institute for Tropical Diseases, 05 01 Chromos, Singapore
    J Biol Chem 286:6233-40. 2011
  7. ncbi Biochemical and genetic characterization of dengue virus methyltransferase
    Hongping Dong
    Novartis Institute for Tropical Diseases, Chromos, Singapore 138670, Singapore
    Virology 405:568-78. 2010
  8. ncbi Peptide inhibitors of Dengue virus NS3 protease. Part 1: Warhead
    Zheng Yin
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore
    Bioorg Med Chem Lett 16:36-9. 2006
  9. ncbi Strategies for development of Dengue virus inhibitors
    Christian G Noble
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore
    Antiviral Res 85:450-62. 2010

Collaborators

Detail Information

Publications9

  1. ncbi A scintillation proximity assay for dengue virus NS5 2'-O-methyltransferase-kinetic and inhibition analyses
    Siew Pheng Lim
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore siew
    Antiviral Res 80:360-9. 2008
    ..It is suitable for determination of enzymatic activities of a wide variety of RNA capping MTases...
  2. ncbi Peptide inhibitors of dengue virus NS3 protease. Part 2: SAR study of tetrapeptide aldehyde inhibitors
    Zheng Yin
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, 138670 Singapore
    Bioorg Med Chem Lett 16:40-3. 2006
    ..Tripeptide and dipeptide aldehyde inhibitors also show low micromolar activity. Additionally, an effective non-basic, uncharged replacement of P(1) Arg is identified...
  3. ncbi Yellow fever virus NS3 protease: peptide-inhibition studies
    Kristina Löhr
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670
    J Gen Virol 88:2223-7. 2007
    ..These findings indicate that the characteristics of YFV protease are very similar to those reported for dengue and West Nile virus proteases, and suggest that pan-flavivirus NS3 protease drugs may be developed for flaviviral diseases...
  4. ncbi Higher catalytic efficiency of N-7-methylation is responsible for processive N-7 and 2'-O methyltransferase activity in dengue virus
    Ka Yan Chung
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, 138670, Singapore
    Virology 402:52-60. 2010
    ..In the context of long viral RNA sequences, and compared to S-adenosyl-homocysteine, sinefungin was about 60- and 12-folds more potent against dengue N-7 and 2'-O MTase activity, exhibiting IC(50) values of 30 and 41nM, respectively...
  5. ncbi Peptide inhibitors of West Nile NS3 protease: SAR study of tetrapeptide aldehyde inhibitors
    John E Knox
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01 Chromos, 138670 Singapore
    J Med Chem 49:6585-90. 2006
    ....
  6. ncbi Small molecule inhibitors that selectively block dengue virus methyltransferase
    Siew Pheng Lim
    Novartis Institute for Tropical Diseases, 05 01 Chromos, Singapore
    J Biol Chem 286:6233-40. 2011
    ..These findings demonstrate that one of the major hurdles for the development of methyltransferase-based therapeutics, namely selectivity for disease-related methyltransferases, can be overcome...
  7. ncbi Biochemical and genetic characterization of dengue virus methyltransferase
    Hongping Dong
    Novartis Institute for Tropical Diseases, Chromos, Singapore 138670, Singapore
    Virology 405:568-78. 2010
    ....
  8. ncbi Peptide inhibitors of Dengue virus NS3 protease. Part 1: Warhead
    Zheng Yin
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore
    Bioorg Med Chem Lett 16:36-9. 2006
    ..Effective inhibition was achieved by peptide inhibitors with electrophilic warheads such as aldehyde, trifluoromethyl ketone, and boronic acid. A boronic acid has the highest affinity, exhibiting a K(i) of 43 nM...
  9. ncbi Strategies for development of Dengue virus inhibitors
    Christian G Noble
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos, Singapore 138670, Singapore
    Antiviral Res 85:450-62. 2010
    ..The strategies discussed in this report should be applicable to antiviral development of other viruses...