Andrew R Collins

Summary

Affiliation: University of Oslo
Country: Norway

Publications

  1. ncbi Oxidation of cellular DNA measured with the comet assay
    Andrew R Collins
    Rowett Research Institute, Aberdeen, UK
    Methods Mol Biol 186:147-59. 2002
  2. ncbi Oxidative stress in humans: validation of biomarkers of DNA damage
    Catherine M Gedik
    Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK
    Carcinogenesis 23:1441-6. 2002
  3. ncbi The comet assay for DNA damage and repair: principles, applications, and limitations
    Andrew R Collins
    Department of Nutrition, University of Oslo, PO Box 1046 Blindern, N 0316 Oslo, Norway
    Mol Biotechnol 26:249-61. 2004
  4. ncbi Assays for oxidative stress and antioxidant status: applications to research into the biological effectiveness of polyphenols
    Andrew R Collins
    Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Norway
    Am J Clin Nutr 81:261S-267S. 2005
  5. ncbi DNA base excision repair as a biomarker in molecular epidemiology studies
    Andrew R Collins
    Department of Nutrition, Faculty of Medicine, University of Oslo, 0316 Oslo, Norway
    Mol Aspects Med 28:307-22. 2007
  6. ncbi Nutritional modulation of DNA repair in a human intervention study
    Andrew R Collins
    Institute for Nutrition Research, University of Oslo, POB 1046 Blindern, N 0316 Oslo, Norway
    Carcinogenesis 24:511-5. 2003
  7. ncbi Are we sure we know how to measure 8-oxo-7,8-dihydroguanine in DNA from human cells?
    Andrew R Collins
    Institute for Nutrition Research, University of Oslo, Norway
    Arch Biochem Biophys 423:57-65. 2004
  8. ncbi Polyphenolic compounds from Salvia species protect cellular DNA from oxidation and stimulate DNA repair in cultured human cells
    Alice A Ramos
    Department of Nutrition, University of Oslo, Oslo, Norway
    J Agric Food Chem 58:7465-71. 2010
  9. doi The comet assay, DNA damage, DNA repair and cytotoxicity: hedgehogs are not always dead
    Yolanda Lorenzo
    Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    Mutagenesis 28:427-32. 2013
  10. doi The carotenoid beta-cryptoxanthin stimulates the repair of DNA oxidation damage in addition to acting as an antioxidant in human cells
    Yolanda Lorenzo
    Department of Nutrition, Faculty of Medicine, University of Oslo, Oslo, Norway
    Carcinogenesis 30:308-14. 2009

Collaborators

Detail Information

Publications46

  1. ncbi Oxidation of cellular DNA measured with the comet assay
    Andrew R Collins
    Rowett Research Institute, Aberdeen, UK
    Methods Mol Biol 186:147-59. 2002
  2. ncbi Oxidative stress in humans: validation of biomarkers of DNA damage
    Catherine M Gedik
    Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK
    Carcinogenesis 23:1441-6. 2002
    ..85, P < 0.05) or by the comet assay (r = 0.86, P < 0.05), although individual values from HPLC and the comet assay did not correlate with each other. It is reasonable to assess oxidative stress by any of these methods...
  3. ncbi The comet assay for DNA damage and repair: principles, applications, and limitations
    Andrew R Collins
    Department of Nutrition, University of Oslo, PO Box 1046 Blindern, N 0316 Oslo, Norway
    Mol Biotechnol 26:249-61. 2004
    ..Alternatively, the repair activity in a cell extract can be measured by incubating it with nucleoids containing specific damage...
  4. ncbi Assays for oxidative stress and antioxidant status: applications to research into the biological effectiveness of polyphenols
    Andrew R Collins
    Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Norway
    Am J Clin Nutr 81:261S-267S. 2005
    ..An increase in the resistance of lymphocyte DNA to oxidation in vitro is commonly seen, however, and a decrease in endogenous oxidation of DNA may be detected, especially after prolonged supplementation...
  5. ncbi DNA base excision repair as a biomarker in molecular epidemiology studies
    Andrew R Collins
    Department of Nutrition, Faculty of Medicine, University of Oslo, 0316 Oslo, Norway
    Mol Aspects Med 28:307-22. 2007
    ..Activity of 8-oxoguanine DNA glycosylase is significantly affected by the Ser326Cys polymorphism in hOGG1. In cancer case-control studies, low repair activity is consistently associated with occurrence of the disease...
  6. ncbi Nutritional modulation of DNA repair in a human intervention study
    Andrew R Collins
    Institute for Nutrition Research, University of Oslo, POB 1046 Blindern, N 0316 Oslo, Norway
    Carcinogenesis 24:511-5. 2003
    ..Kiwifruit provides a dual protection against oxidative DNA damage, enhancing antioxidant levels and stimulating DNA repair. It is probable that together these effects would decrease the risk of mutagenic changes leading to cancer...
  7. ncbi Are we sure we know how to measure 8-oxo-7,8-dihydroguanine in DNA from human cells?
    Andrew R Collins
    Institute for Nutrition Research, University of Oslo, Norway
    Arch Biochem Biophys 423:57-65. 2004
    ..The background level of DNA oxidation in normal human cells is likely to be around 0.3-4.2 8-oxoGua per 10(6) Gua. An effort should be made to develop alternative, validated methods for estimating oxidative DNA damage...
  8. ncbi Polyphenolic compounds from Salvia species protect cellular DNA from oxidation and stimulate DNA repair in cultured human cells
    Alice A Ramos
    Department of Nutrition, University of Oslo, Oslo, Norway
    J Agric Food Chem 58:7465-71. 2010
    ..In conclusion, SO, SF, and the isolated compounds (RA and L-7-G) demonstrated chemopreventive activity by protecting cells against oxidative DNA damage and stimulating DNA repair (SO, SF, and L-7-G)...
  9. doi The comet assay, DNA damage, DNA repair and cytotoxicity: hedgehogs are not always dead
    Yolanda Lorenzo
    Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    Mutagenesis 28:427-32. 2013
    ..It is clear that 'hedgehogs' can correspond to one level on a continuum of genotoxic damage, are not diagnostic of apoptosis and should not be regarded as an indicator of cytotoxicity...
  10. doi The carotenoid beta-cryptoxanthin stimulates the repair of DNA oxidation damage in addition to acting as an antioxidant in human cells
    Yolanda Lorenzo
    Department of Nutrition, Faculty of Medicine, University of Oslo, Oslo, Norway
    Carcinogenesis 30:308-14. 2009
    ..The apparent cancer-preventive effects of dietary carotenoids may depend on the enhancement of DNA repair as well as antioxidant protection against damage...
  11. doi Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisation
    Sergey Shaposhnikov
    Department of Nutrition, University of Oslo, Blindern, Oslo, Norway
    Toxicol Lett 195:31-4. 2010
    ....
  12. doi A comparative performance test of standard, medium- and high-throughput comet assays
    Amaya Azqueta
    Department of Nutrition, Faculty of Medicine, University of Oslo, P O Box 1046, Blindern, 0316 Oslo, Norway
    Toxicol In Vitro 27:768-73. 2013
    ....
  13. ncbi Towards a more reliable comet assay: optimising agarose concentration, unwinding time and electrophoresis conditions
    Amaya Azqueta
    Department of Nutrition, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway
    Mutat Res 724:41-5. 2011
    ..human peripheral blood lymphocytes and the lymphoblastoid cell line TK-6. All these variables have marked effects on assay performance and, therefore, on the determination of DNA damage. Here we identify factors of particular importance...
  14. doi DNA oxidation: investigating its key role in environmental mutagenesis with the comet assay
    Amaya Azqueta
    Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway
    Mutat Res 674:101-8. 2009
    ..Finally, the potential use of this technique to monitor effects of contaminants in the natural environment has yet to be fully exploited...
  15. doi DNA damage in lens epithelium of cataract patients in vivo and ex vivo
    Oyvind Øsnes-Ringen
    Department of Ophthalmology, Center for Eye Research, Oslo University Hospital, University of Oslo, Oslo, NorwayDepartment of Nutrition, Institute for Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Pathology, Oslo University Hospital, University of Oslo, Oslo, Norway
    Acta Ophthalmol 91:652-6. 2013
    ..In this study, the aim was to explore the quality and relative quantity of DNA damage in lens epithelium of cataract patients in vivo and after incubation in a cell culture system...
  16. doi DNA repair as a biomarker in human biomonitoring studies; further applications of the comet assay
    Andrew R Collins
    Department of Nutrition, University of Oslo, Oslo, Norway
    Mutat Res 736:122-9. 2012
    ..Exposure to genotoxic agents has in general not been found to stimulate repair. Crucial questions concerning the factors regulating repair and the causes of individual variation are as yet unanswered...
  17. doi Effects of micronutrients on DNA repair
    Andrew R Collins
    Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Blindern, PB 1046, 0316, Oslo, Norway
    Eur J Nutr 51:261-79. 2012
    ..In addition, there are numerous investigations at the level of expression of DNA repair-related genes...
  18. ncbi Comet assay-based methods for measuring DNA repair in vitro; estimates of inter- and intra-individual variation
    Isabel Gaivão
    Department of Nutrition, Faculty of Medicine, University of Oslo, PB 1046, Blindern, 0316 Oslo, Norway
    Cell Biol Toxicol 25:45-52. 2009
    ..There was no correlation between BER and NER rates. The BER and NER assays are simple to perform and can provide valuable information in molecular epidemiological studies in which DNA instability is an endpoint...
  19. ncbi Investigating oxidative DNA damage and its repair using the comet assay
    Andrew R Collins
    Department of Nutrition, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway
    Mutat Res 681:24-32. 2009
    ....
  20. ncbi In vitro comet assay for DNA repair: a warning concerning application to cultured cells
    Amaya Azqueta
    Department of Nutrition, University of Oslo, Norway
    Mutagenesis 24:379-81. 2009
    ..Here, we describe a rigorous approach to testing for this possibility, which we recommend to anyone carrying out similar experiments...
  21. doi Increasing the resolution of the comet assay using fluorescent in situ hybridization--a review
    Sergey Shaposhnikov
    Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway
    Mutagenesis 24:383-9. 2009
    ..Here, we provide a general review of the technique, answer some technical and theoretical questions and give examples of applications of the method...
  22. doi The use of bacterial repair endonucleases in the comet assay
    Andrew R Collins
    Department of Nutrition, Faculty of Medicine, University of Oslo, Oslo, Norway
    Methods Mol Biol 691:137-47. 2011
    ..The additional enzyme-sensitive sites, over and above the strand breaks detected in the standard comet assay, give a quantitative estimate of the number of specific lesions present in the cells...
  23. doi Combining fluorescent in situ hybridization with the comet assay for targeted examination of DNA damage and repair
    Sergey Shaposhnikov
    Department of Nutrition, Faculty of Medicine, University of Oslo, Oslo, Norway
    Methods Mol Biol 682:115-32. 2011
    ..Here we provide protocols for the comet assay and the FISH modification, answer some technical questions, and give examples of applications of the technique...
  24. doi Protective effects of ursolic acid and luteolin against oxidative DNA damage include enhancement of DNA repair in Caco-2 cells
    Alice A Ramos
    Department of Nutrition, University of Oslo, Norway
    Mutat Res 692:6-11. 2010
    ..These effects of ursolic acid and luteolin may contribute to their anti-carcinogenic effects...
  25. doi Measuring oxidative damage to DNA and its repair with the comet assay
    Andrew R Collins
    Department of Nutrition, University of Oslo, Norway Electronic address
    Biochim Biophys Acta 1840:794-800. 2014
    ..A simple modification, incorporating a digestion of DNA with a lesion-specific endonuclease, makes it possible to measure oxidised bases...
  26. doi Single-cell gel electrophoresis (the comet assay): loops or fragments?
    Sergey A Shaposhnikov
    Department of Nutrition, Faculty of Medicine, University of Oslo, Oslo, Norway
    Electrophoresis 29:3005-12. 2008
    ..After alkaline electrophoresis, the appearance of the fluorescent probes suggests that linear DNA has coalesced into a granular form...
  27. ncbi Antioxidant intervention as a route to cancer prevention
    Andrew R Collins
    Department of Nutrition, University of Oslo, PO Box 1046 Blindern, 0316 Oslo, Norway
    Eur J Cancer 41:1923-30. 2005
    ..It is certainly true that we do not yet fully understand the role of phytochemicals as antioxidants, or as modulators of other processes related to carcinogenesis and its prevention...
  28. doi Development of a new application of the comet assay to assess levels of O6-methylguanine in genomic DNA (CoMeth)
    Alice A Ramos
    Center of Molecular and Environmental Biology, Department of Biology, School of Sciences, University of Minho, 4710 057 Braga, Portugal
    Free Radic Biol Med 60:41-8. 2013
    ..This proliferating cell system complements other methodologies that look at effects on these parameters individually through analytical chemistry or in vitro assays with recombinant proteins...
  29. doi The comet assay: topical issues
    Andrew R Collins
    Department of Nutrition, Faculty of Medicine, University of Oslo, Oslo, Norway
    Mutagenesis 23:143-51. 2008
    ..Arguments are presented against trying to apply the comet assay to the study of apoptosis. Finally, some of the advantages and disadvantages of using the comet assay on lymphocyte samples collected in human studies are rehearsed...
  30. ncbi A map of nuclear matrix attachment regions within the breast cancer loss-of-heterozygosity region on human chromosome 16q22.1
    Sergey A Shaposhnikov
    Department of Nutrition, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway
    Genomics 89:354-61. 2007
    ..The S/MAR resource constructed may contribute to an understanding of how the genes in the region are regulated and of how the structural architecture and functional organization of the DNA are related...
  31. doi Occupational exposure to mineral fibres. Biomarkers of oxidative damage and antioxidant defence and associations with DNA damage and repair
    Marta Staruchova
    Research Base of Slovak Medical University, Limbova 12, 833 03 Bratislava, Slovakia
    Mutagenesis 23:249-60. 2008
    ..Our study supports the idea that there might also be other mechanisms by which antioxidant enzymes (especially GST) protect cells against oxidative DNA damage...
  32. ncbi Does a vegetarian diet influence genomic stability?
    Alena Kazimirova
    Institute of Preventive and Clinical Medicine, Limbova 14, 83301, Bratislava, Slovakia
    Eur J Nutr 43:32-8. 2004
    ..The vegetarian lifestyle is supposedly healthy, and differences between vegetarians and non-vegetarians in biomarkers related to diseases such as cancer might be expected...
  33. doi The comet assay in human biomonitoring: gene-environment interactions
    Maria Dusinska
    Centre for Ecological Economics, Norwegian Institute of Air Research NILU, Kjeller, Norway
    Mutagenesis 23:191-205. 2008
    ..The information obtained could lead to individual advice on lifestyle changes to promote health and especially on relative risks of genotoxic exposure to environmental pollution...
  34. ncbi Possible involvement of XPA in repair of oxidative DNA damage deduced from analysis of damage, repair and genotype in a human population study
    Maria Dusinska
    Department of Experimental and Applied Medicine, Institute of Preventive and Clinical Medicine, Research Base of Slovak Medical University Limbová 14, Bratislava, Slovakia
    Mutagenesis 21:205-11. 2006
    ..Although XPA is known as a protein involved in nucleotide excision repair of UV-induced damage and bulky DNA adducts, it may also have a role in the repair of oxidized bases...
  35. ncbi The comet assay. Principles, applications, and limitations
    Andrew R Collins
    DNA Instability Laboratory, Rowett Research Institute, Aberdeen, UK
    Methods Mol Biol 203:163-77. 2002
  36. ncbi Antioxidant enzyme activities, lipid peroxidation, and DNA oxidative damage: the effects of short-term voluntary wheel running
    Colin Selman
    Aberdeen Centre for Energy Expenditure and Obesity ACERO, Department of Zoology, Aberdeen University, Aberdeen AB24 2TZ, Scotland, UK
    Arch Biochem Biophys 401:255-61. 2002
    ..There was no evidence of any significant increase in any oxidative stress parameter in running individuals, despite having significantly elevated energy expenditures compared to sedentary controls...
  37. ncbi Age-related increases in DNA repair and antioxidant protection: a comparison of the Boyd Orr Cohort of elderly subjects with a younger population sample
    Vikki Humphreys
    The Robert Gordon University, St Andrew Street, Aberdeen AB25 1HG, UK
    Age Ageing 36:521-6. 2007
    ..Accumulation of oxidative DNA damage with age will occur if there is an increase in reactive oxygen species in the body, or a decline in antioxidant defences, or a reduced efficiency of DNA repair...
  38. ncbi DNA damage and repair measured in different genomic regions using the comet assay with fluorescent in situ hybridization
    Eva Horvathova
    Cancer Research Institute, Vlarska 7, 833 91 Bratislava, Slovakia, Institute of Preventive and Clinical Medicine, Slovak Medical University, Limbova 12, 833 03 Bratislava, Slovakia
    Mutagenesis 19:269-76. 2004
    ..Thus, fluorescent in situ hybridization in combination with the comet assay provides a powerful method for studying repair of specific genes in relation to chromatin structure...
  39. ncbi Nutrition and carcinogenesis
    Andrew R Collins
    Mutat Res 551:1-8. 2004
    ..The process is modulated by the immune system, and genetics plays a significant role. New monitoring methods provide much-needed tools for providing proof of involvement of various factors at the level of human populations...
  40. ncbi Are environmental electromagnetic fields genotoxic?
    Michael J Crumpton
    Scientific Advisory Committee, The EMF Biological Research Trust, P O Box 23, S Croydon CR2 7ZL, UK
    DNA Repair (Amst) 3:1385-7. 2004
    ..The implications of these findings are discussed...
  41. doi Lifelong alpha-tocopherol supplementation increases the median life span of C57BL/6 mice in the cold but has only minor effects on oxidative damage
    Colin Selman
    Integrative Physiology, School of Biological Sciences, University of Aberdeen, Aberdeen, United Kingdom
    Rejuvenation Res 11:83-96. 2008
    ..However, these signaling pathways now require further investigation to establish their exact role in life span extension following alpha-tocopherol supplementation...
  42. ncbi Life-long vitamin C supplementation in combination with cold exposure does not affect oxidative damage or lifespan in mice, but decreases expression of antioxidant protection genes
    Colin Selman
    Aberdeen Centre for Energy Regulation and Obesity ACERO, School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK
    Mech Ageing Dev 127:897-904. 2006
    ....
  43. ncbi Fourth International Workgroup on Genotoxicity testing: results of the in vivo Comet assay workgroup
    Brian Burlinson
    Huntingdon Life Sciences, Cellular and Molecular Toxicology, Woolley Road, Alconbury, Huntington, Cambs PE28 4HS, UK
    Mutat Res 627:31-5. 2007
    ..It was also agreed that a minimum reporting standard would be developed which would be consistent with OECD in vivo genotoxicity test method guidelines...
  44. pmc The impact of experimentally elevated energy expenditure on oxidative stress and lifespan in the short-tailed field vole Microtus agrestis
    Colin Selman
    Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK
    Proc Biol Sci 275:1907-16. 2008
    ..These data suggest that in captive animals physiologically mediated costs on life history do not result from increased energy expenditure and consequent elevations in oxidative stress and reduced survival...
  45. pmc Extent of genome-wide linkage disequilibrium in Australian Holstein-Friesian cattle based on a high-density SNP panel
    Mehar S Khatkar
    Centre for Advanced Technologies in Animal Genetics and Reproduction REPROGEN, University of Sydney, Camden, NSW 2570, Australia
    BMC Genomics 9:187. 2008
    ..Finally, relatively little information is available on comparative LD structures including other mammalian species such as human and mouse, and we compare LD structure in cattle with public-domain data from both human and mouse...
  46. ncbi Voluntary exercise has only limited effects on activity of antioxidant enzymes and does not cause oxidative damage in a small mammal
    Colin Selman
    Aberdeen Centre for Energy Expenditure and Obesity, Department of Zoology, Aberdeen University, Aberdeen, UK
    J Nutr 132:1784S-6S. 2002