Research Topics
| Andrew R CollinsSummaryAffiliation: University of Oslo Country: Norway Publications
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Detail Information
Publications
Age-related increases in DNA repair and antioxidant protection: a comparison of the Boyd Orr Cohort of elderly subjects with a younger population sampleVikki Humphreys
The Robert Gordon University, St Andrew Street, Aberdeen AB25 1HG, UK
Age Ageing 36:521-6. 2007..Accumulation of oxidative DNA damage with age will occur if there is an increase in reactive oxygen species in the body, or a decline in antioxidant defences, or a reduced efficiency of DNA repair...- Oxidation of cellular DNA measured with the comet assayAndrew R Collins
Rowett Research Institute, Aberdeen, UK
Methods Mol Biol 186:147-59. 2002 - Oxidative stress in humans: validation of biomarkers of DNA damageCatherine M Gedik
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK
Carcinogenesis 23:1441-6. 2002..85, P < 0.05) or by the comet assay (r = 0.86, P < 0.05), although individual values from HPLC and the comet assay did not correlate with each other. It is reasonable to assess oxidative stress by any of these methods... - The comet assay for DNA damage and repair: principles, applications, and limitationsAndrew R Collins
Department of Nutrition, University of Oslo, PO Box 1046 Blindern, N 0316 Oslo, Norway
Mol Biotechnol 26:249-61. 2004..Alternatively, the repair activity in a cell extract can be measured by incubating it with nucleoids containing specific damage... - Assays for oxidative stress and antioxidant status: applications to research into the biological effectiveness of polyphenolsAndrew R Collins
Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Norway
Am J Clin Nutr 81:261S-267S. 2005..An increase in the resistance of lymphocyte DNA to oxidation in vitro is commonly seen, however, and a decrease in endogenous oxidation of DNA may be detected, especially after prolonged supplementation... - Nutritional modulation of DNA repair in a human intervention studyAndrew R Collins
Institute for Nutrition Research, University of Oslo, POB 1046 Blindern, N 0316 Oslo, Norway
Carcinogenesis 24:511-5. 2003..Kiwifruit provides a dual protection against oxidative DNA damage, enhancing antioxidant levels and stimulating DNA repair. It is probable that together these effects would decrease the risk of mutagenic changes leading to cancer... - Are we sure we know how to measure 8-oxo-7,8-dihydroguanine in DNA from human cells?Andrew R Collins
Institute for Nutrition Research, University of Oslo, Norway
Arch Biochem Biophys 423:57-65. 2004..The background level of DNA oxidation in normal human cells is likely to be around 0.3-4.2 8-oxoGua per 10(6) Gua. An effort should be made to develop alternative, validated methods for estimating oxidative DNA damage... 
Polyphenolic compounds from Salvia species protect cellular DNA from oxidation and stimulate DNA repair in cultured human cellsAlice A Ramos
Department of Nutrition, University of Oslo, Oslo, Norway
J Agric Food Chem 58:7465-71. 2010..In conclusion, SO, SF, and the isolated compounds (RA and L-7-G) demonstrated chemopreventive activity by protecting cells against oxidative DNA damage and stimulating DNA repair (SO, SF, and L-7-G)...- The carotenoid beta-cryptoxanthin stimulates the repair of DNA oxidation damage in addition to acting as an antioxidant in human cellsYolanda Lorenzo
Department of Nutrition, Faculty of Medicine, University of Oslo, Oslo, Norway
Carcinogenesis 30:308-14. 2009..The apparent cancer-preventive effects of dietary carotenoids may depend on the enhancement of DNA repair as well as antioxidant protection against damage... - Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisationSergey Shaposhnikov
Department of Nutrition, University of Oslo, Blindern, Oslo, Norway
Toxicol Lett 195:31-4. 2010.... - DNA base excision repair as a biomarker in molecular epidemiology studiesAndrew R Collins
Department of Nutrition, Faculty of Medicine, University of Oslo, 0316 Oslo, Norway
Mol Aspects Med 28:307-22. 2007..Activity of 8-oxoguanine DNA glycosylase is significantly affected by the Ser326Cys polymorphism in hOGG1. In cancer case-control studies, low repair activity is consistently associated with occurrence of the disease... - Towards a more reliable comet assay: optimising agarose concentration, unwinding time and electrophoresis conditionsAmaya Azqueta
Department of Nutrition, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway
Mutat Res 724:41-5. 2011..human peripheral blood lymphocytes and the lymphoblastoid cell line TK-6. All these variables have marked effects on assay performance and, therefore, on the determination of DNA damage. Here we identify factors of particular importance... - DNA oxidation: investigating its key role in environmental mutagenesis with the comet assayAmaya Azqueta
Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway
Mutat Res 674:101-8. 2009..Finally, the potential use of this technique to monitor effects of contaminants in the natural environment has yet to be fully exploited... - DNA repair as a biomarker in human biomonitoring studies; further applications of the comet assayAndrew R Collins
Department of Nutrition, University of Oslo, Oslo, Norway
Mutat Res 736:122-9. 2012..Exposure to genotoxic agents has in general not been found to stimulate repair. Crucial questions concerning the factors regulating repair and the causes of individual variation are as yet unanswered... - Effects of micronutrients on DNA repairAndrew R Collins
Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Blindern, PB 1046, 0316, Oslo, Norway
Eur J Nutr 51:261-79. 2012..In addition, there are numerous investigations at the level of expression of DNA repair-related genes... - In vitro comet assay for DNA repair: a warning concerning application to cultured cellsAmaya Azqueta
Department of Nutrition, University of Oslo, Norway
Mutagenesis 24:379-81. 2009..Here, we describe a rigorous approach to testing for this possibility, which we recommend to anyone carrying out similar experiments... - Combining fluorescent in situ hybridization with the comet assay for targeted examination of DNA damage and repairSergey Shaposhnikov
Department of Nutrition, Faculty of Medicine, University of Oslo, Oslo, Norway
Methods Mol Biol 682:115-32. 2011..Here we provide protocols for the comet assay and the FISH modification, answer some technical questions, and give examples of applications of the technique... - Increasing the resolution of the comet assay using fluorescent in situ hybridization--a reviewSergey Shaposhnikov
Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway
Mutagenesis 24:383-9. 2009..Here, we provide a general review of the technique, answer some technical and theoretical questions and give examples of applications of the method... - The use of bacterial repair endonucleases in the comet assayAndrew R Collins
Department of Nutrition, Faculty of Medicine, University of Oslo, Oslo, Norway
Methods Mol Biol 691:137-47. 2011..The additional enzyme-sensitive sites, over and above the strand breaks detected in the standard comet assay, give a quantitative estimate of the number of specific lesions present in the cells... - Comet assay-based methods for measuring DNA repair in vitro; estimates of inter- and intra-individual variationIsabel Gaivão
Department of Nutrition, Faculty of Medicine, University of Oslo, PB 1046, Blindern, 0316 Oslo, Norway
Cell Biol Toxicol 25:45-52. 2009..There was no correlation between BER and NER rates. The BER and NER assays are simple to perform and can provide valuable information in molecular epidemiological studies in which DNA instability is an endpoint... - Investigating oxidative DNA damage and its repair using the comet assayAndrew R Collins
Department of Nutrition, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway
Mutat Res 681:24-32. 2009.... - Protective effects of ursolic acid and luteolin against oxidative DNA damage include enhancement of DNA repair in Caco-2 cellsAlice A Ramos
Department of Nutrition, University of Oslo, Norway
Mutat Res 692:6-11. 2010..These effects of ursolic acid and luteolin may contribute to their anti-carcinogenic effects... - A comparative performance test of standard, medium- and high-throughput comet assaysAmaya Azqueta
Department of Nutrition, Faculty of Medicine, University of Oslo, P O Box 1046, Blindern, 0316 Oslo, Norway
Toxicol In Vitro 27:768-73. 2013.... - Single-cell gel electrophoresis (the comet assay): loops or fragments?Sergey A Shaposhnikov
Department of Nutrition, Faculty of Medicine, University of Oslo, Oslo, Norway
Electrophoresis 29:3005-12. 2008..After alkaline electrophoresis, the appearance of the fluorescent probes suggests that linear DNA has coalesced into a granular form... - Antioxidant intervention as a route to cancer preventionAndrew R Collins
Department of Nutrition, University of Oslo, PO Box 1046 Blindern, 0316 Oslo, Norway
Eur J Cancer 41:1923-30. 2005..It is certainly true that we do not yet fully understand the role of phytochemicals as antioxidants, or as modulators of other processes related to carcinogenesis and its prevention... - The comet assay: topical issuesAndrew R Collins
Department of Nutrition, Faculty of Medicine, University of Oslo, Oslo, Norway
Mutagenesis 23:143-51. 2008..Arguments are presented against trying to apply the comet assay to the study of apoptosis. Finally, some of the advantages and disadvantages of using the comet assay on lymphocyte samples collected in human studies are rehearsed... - A map of nuclear matrix attachment regions within the breast cancer loss-of-heterozygosity region on human chromosome 16q22.1Sergey A Shaposhnikov
Department of Nutrition, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway
Genomics 89:354-61. 2007..The S/MAR resource constructed may contribute to an understanding of how the genes in the region are regulated and of how the structural architecture and functional organization of the DNA are related... - The comet assay in human biomonitoring: gene-environment interactionsMaria Dusinska
Centre for Ecological Economics, Norwegian Institute of Air Research NILU, Kjeller, Norway
Mutagenesis 23:191-205. 2008..The information obtained could lead to individual advice on lifestyle changes to promote health and especially on relative risks of genotoxic exposure to environmental pollution... - Occupational exposure to mineral fibres. Biomarkers of oxidative damage and antioxidant defence and associations with DNA damage and repairMarta Staruchova
Research Base of Slovak Medical University, Limbova 12, 833 03 Bratislava, Slovakia
Mutagenesis 23:249-60. 2008..Our study supports the idea that there might also be other mechanisms by which antioxidant enzymes (especially GST) protect cells against oxidative DNA damage... - Possible involvement of XPA in repair of oxidative DNA damage deduced from analysis of damage, repair and genotype in a human population studyMaria Dusinska
Department of Experimental and Applied Medicine, Institute of Preventive and Clinical Medicine, Research Base of Slovak Medical University Limbová 14, Bratislava, Slovakia
Mutagenesis 21:205-11. 2006..Although XPA is known as a protein involved in nucleotide excision repair of UV-induced damage and bulky DNA adducts, it may also have a role in the repair of oxidized bases... - Does a vegetarian diet influence genomic stability?Alena Kazimirova
Institute of Preventive and Clinical Medicine, Limbova 14, 83301, Bratislava, Slovakia
Eur J Nutr 43:32-8. 2004..The vegetarian lifestyle is supposedly healthy, and differences between vegetarians and non-vegetarians in biomarkers related to diseases such as cancer might be expected... - The comet assay. Principles, applications, and limitationsAndrew R Collins
DNA Instability Laboratory, Rowett Research Institute, Aberdeen, UK
Methods Mol Biol 203:163-77. 2002 - Antioxidant enzyme activities, lipid peroxidation, and DNA oxidative damage: the effects of short-term voluntary wheel runningColin Selman
Aberdeen Centre for Energy Expenditure and Obesity ACERO, Department of Zoology, Aberdeen University, Aberdeen AB24 2TZ, Scotland, UK
Arch Biochem Biophys 401:255-61. 2002..There was no evidence of any significant increase in any oxidative stress parameter in running individuals, despite having significantly elevated energy expenditures compared to sedentary controls... - DNA damage and repair measured in different genomic regions using the comet assay with fluorescent in situ hybridizationEva Horvathova
Cancer Research Institute, Vlarska 7, 833 91 Bratislava, Slovakia, Institute of Preventive and Clinical Medicine, Slovak Medical University, Limbova 12, 833 03 Bratislava, Slovakia
Mutagenesis 19:269-76. 2004..Thus, fluorescent in situ hybridization in combination with the comet assay provides a powerful method for studying repair of specific genes in relation to chromatin structure... - Fourth International Workgroup on Genotoxicity testing: results of the in vivo Comet assay workgroupBrian Burlinson
Huntingdon Life Sciences, Cellular and Molecular Toxicology, Woolley Road, Alconbury, Huntington, Cambs PE28 4HS, UK
Mutat Res 627:31-5. 2007..It was also agreed that a minimum reporting standard would be developed which would be consistent with OECD in vivo genotoxicity test method guidelines... - Nutrition and carcinogenesisAndrew R Collins
Mutat Res 551:1-8. 2004..The process is modulated by the immune system, and genetics plays a significant role. New monitoring methods provide much-needed tools for providing proof of involvement of various factors at the level of human populations... - Are environmental electromagnetic fields genotoxic?Michael J Crumpton
Scientific Advisory Committee, The EMF Biological Research Trust, P O Box 23, S Croydon CR2 7ZL, UK
DNA Repair (Amst) 3:1385-7. 2004..The implications of these findings are discussed... - Life-long vitamin C supplementation in combination with cold exposure does not affect oxidative damage or lifespan in mice, but decreases expression of antioxidant protection genesColin Selman
Aberdeen Centre for Energy Regulation and Obesity ACERO, School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK
Mech Ageing Dev 127:897-904. 2006.... - Lifelong alpha-tocopherol supplementation increases the median life span of C57BL/6 mice in the cold but has only minor effects on oxidative damageColin Selman
Integrative Physiology, School of Biological Sciences, University of Aberdeen, Aberdeen, United Kingdom
Rejuvenation Res 11:83-96. 2008..However, these signaling pathways now require further investigation to establish their exact role in life span extension following alpha-tocopherol supplementation... 
The impact of experimentally elevated energy expenditure on oxidative stress and lifespan in the short-tailed field vole Microtus agrestisColin Selman
Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK
Proc Biol Sci 275:1907-16. 2008..These data suggest that in captive animals physiologically mediated costs on life history do not result from increased energy expenditure and consequent elevations in oxidative stress and reduced survival...- Extent of genome-wide linkage disequilibrium in Australian Holstein-Friesian cattle based on a high-density SNP panelMehar S Khatkar
Centre for Advanced Technologies in Animal Genetics and Reproduction REPROGEN, University of Sydney, Camden, NSW 2570, Australia
BMC Genomics 9:187. 2008..Finally, relatively little information is available on comparative LD structures including other mammalian species such as human and mouse, and we compare LD structure in cattle with public-domain data from both human and mouse... - Voluntary exercise has only limited effects on activity of antioxidant enzymes and does not cause oxidative damage in a small mammalColin Selman
Aberdeen Centre for Energy Expenditure and Obesity, Department of Zoology, Aberdeen University, Aberdeen, UK
J Nutr 132:1784S-6S. 2002