Philippe Collas

Summary

Affiliation: University of Oslo
Country: Norway

Publications

  1. pmc Histone H3 lysine 27 methylation asymmetry on developmentally-regulated promoters distinguish the first two lineages in mouse preimplantation embryos
    John Arne Dahl
    Institute of Basic Medical Sciences, University of Oslo and Norwegian Center for Stem Cell Research, Oslo, Norway
    PLoS ONE 5:e9150. 2010
  2. pmc MicroChIP--a rapid micro chromatin immunoprecipitation assay for small cell samples and biopsies
    John Arne Dahl
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway
    Nucleic Acids Res 36:e15. 2008
  3. pmc Tiling histone H3 lysine 4 and 27 methylation in zebrafish using high-density microarrays
    Leif C Lindeman
    Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, and Norwegian Center for Stem Cell Research, Oslo, Norway
    PLoS ONE 5:e15651. 2010
  4. pmc Genome-wide map of quantified epigenetic changes during in vitro chondrogenic differentiation of primary human mesenchymal stem cells
    Sarah R Herlofsen
    Institute of Immunology and Norwegian Center for Stem Cell Research, Oslo University Hospital Rikshospitalet, Oslo 0424, Norway
    BMC Genomics 14:105. 2013
  5. ncbi request reprint Oslo Epigenetics Symposium 2012. Oslo, Norway, 8-9 November 2012
    Philippe Collas
    Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    Epigenomics 5:29-32. 2013
  6. doi request reprint The current state of chromatin immunoprecipitation
    Philippe Collas
    Institute of Basic Medical Sciences, Faculty of Medicine, Norwegian Center for Stem Cell Research, University of Oslo, Oslo, Norway
    Mol Biotechnol 45:87-100. 2010
  7. doi request reprint Epigenetic states in stem cells
    Philippe Collas
    Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, Norway
    Biochim Biophys Acta 1790:900-5. 2009
  8. ncbi request reprint On the way to reprogramming cells to pluripotency using cell-free extracts
    Philippe Collas
    Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
    Reprod Biomed Online 12:762-70. 2006
  9. ncbi request reprint Chop it, ChIP it, check it: the current status of chromatin immunoprecipitation
    Philippe Collas
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
    Front Biosci 13:929-43. 2008
  10. ncbi request reprint Epigenetic reprogramming of nuclei using cell extracts
    Philippe Collas
    Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, Blindern, Oslo 0317, Norway
    Stem Cell Rev 2:309-17. 2006

Collaborators

Detail Information

Publications79

  1. pmc Histone H3 lysine 27 methylation asymmetry on developmentally-regulated promoters distinguish the first two lineages in mouse preimplantation embryos
    John Arne Dahl
    Institute of Basic Medical Sciences, University of Oslo and Norwegian Center for Stem Cell Research, Oslo, Norway
    PLoS ONE 5:e9150. 2010
    ..Our results show histone trimethylation asymmetry on promoters in the first two developmental lineages, and highlight an epigenetic skewing associated with embryonic stem cell derivation...
  2. pmc MicroChIP--a rapid micro chromatin immunoprecipitation assay for small cell samples and biopsies
    John Arne Dahl
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway
    Nucleic Acids Res 36:e15. 2008
    ..muChIP creates possibilities for multiple parallel and rapid transcription factor binding and epigenetic analyses of rare cell and tissue samples...
  3. pmc Tiling histone H3 lysine 4 and 27 methylation in zebrafish using high-density microarrays
    Leif C Lindeman
    Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, and Norwegian Center for Stem Cell Research, Oslo, Norway
    PLoS ONE 5:e15651. 2010
    ..Many studies have been carried out in mice and humans; however limited high-resolution information exists to date for non-mammalian vertebrate species...
  4. pmc Genome-wide map of quantified epigenetic changes during in vitro chondrogenic differentiation of primary human mesenchymal stem cells
    Sarah R Herlofsen
    Institute of Immunology and Norwegian Center for Stem Cell Research, Oslo University Hospital Rikshospitalet, Oslo 0424, Norway
    BMC Genomics 14:105. 2013
    ..To add to the clinical relevance of our observations, the study is based on primary bone marrow-derived MSCs from four donors, allowing us to investigate inter-individual variations...
  5. ncbi request reprint Oslo Epigenetics Symposium 2012. Oslo, Norway, 8-9 November 2012
    Philippe Collas
    Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    Epigenomics 5:29-32. 2013
    ....
  6. doi request reprint The current state of chromatin immunoprecipitation
    Philippe Collas
    Institute of Basic Medical Sciences, Faculty of Medicine, Norwegian Center for Stem Cell Research, University of Oslo, Oslo, Norway
    Mol Biotechnol 45:87-100. 2010
    ..This review highlights the variations on the theme of the ChIP assay, the various detection methods applied downstream of ChIP, and examples of their application...
  7. doi request reprint Epigenetic states in stem cells
    Philippe Collas
    Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, Norway
    Biochim Biophys Acta 1790:900-5. 2009
    ..We summarize here the current view of how specific combinations of epigenetic marks may define the pluripotent state...
  8. ncbi request reprint On the way to reprogramming cells to pluripotency using cell-free extracts
    Philippe Collas
    Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
    Reprod Biomed Online 12:762-70. 2006
    ..These approaches include the fusion of differentiated cells with embryonic stem cells and the use of extract from pluripotent cells to reprogramme differentiated cells into multipotent or pluripotent cells...
  9. ncbi request reprint Chop it, ChIP it, check it: the current status of chromatin immunoprecipitation
    Philippe Collas
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
    Front Biosci 13:929-43. 2008
    ..Finally, we speculate on future perspectives opened by the combination of emerging ChIP-related technologies...
  10. ncbi request reprint Epigenetic reprogramming of nuclei using cell extracts
    Philippe Collas
    Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, Blindern, Oslo 0317, Norway
    Stem Cell Rev 2:309-17. 2006
    ..Limitations of current extract-based reprogramming approaches are also addressed...
  11. ncbi request reprint Programming the genome in embryonic and somatic stem cells
    Philippe Collas
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway
    J Cell Mol Med 11:602-20. 2007
    ..The combinatorial association of epigenetic marks on developmentally regulated and lineage-specifying genes in undifferentiated cells seems to define a pluripotent state...
  12. doi request reprint A chromatin immunoprecipitation protocol for small cell numbers
    Philippe Collas
    University of Oslo, Institute of Basic Medical Sciences and Norwegian Center for Stem Cell Research, Oslo, Norway
    Methods Mol Biol 791:179-93. 2011
    ..This chapter describes a micro (μ)ChIP procedure for multiple parallel ChIPs from a single chromatin batch from 1,000 cells...
  13. doi request reprint Programming differentiation potential in mesenchymal stem cells
    Philippe Collas
    Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway
    Epigenetics 5:476-82. 2010
    ..Additional regulatory layers need to be examined to be able to explain cell fate commitment and ultimately predict cell fate...
  14. doi request reprint Chromatin states of developmentally-regulated genes revealed by DNA and histone methylation patterns in zebrafish embryos
    Leif C Lindeman
    Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, and Norwegian Center for Stem Cell Research, Oslo, Norway
    Int J Dev Biol 54:803-13. 2010
    ..Our results suggest that differentially expressed embryonic genes are regulated by various patterns of histone modifications on unmethylated DNA, which create a developmentally permissive chromatin state...
  15. pmc Promoter DNA methylation patterns of differentiated cells are largely programmed at the progenitor stage
    Anita L Sørensen
    Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, and Norwegian Center for Stem Cell Research, 0317 Oslo, Norway
    Mol Biol Cell 21:2066-77. 2010
    ..We infer that methylation state of lineage-specific promoters in MSCs is not a primary determinant of differentiation capacity. Our results support the view of a common origin of mesenchymal progenitors...
  16. doi request reprint Histone H3 modifications associated with differentiation and long-term culture of mesenchymal adipose stem cells
    Agate Noer
    Department of Biochemistry, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway
    Stem Cells Dev 18:725-36. 2009
    ..This correlates with global cellular Ezh2 increase and H3K9 deacetylation. Promoter targeting by Ezh2 and Bmi1 in late passage ASCs suggests the establishment of a polycomb-mediated epigenetic program aiming at repressing transcription...
  17. ncbi request reprint Protein kinase A-anchoring protein AKAP95 interacts with MCM2, a regulator of DNA replication
    Turid Eide
    Department of Medical Biochemistry, University of Oslo, P O Box 1112 Blindern, 0317 Oslo, Norway
    J Biol Chem 278:26750-6. 2003
    ..Recombinant AKAP95 restores intranuclear MCM2 and replication in a dose-dependent manner. Our results suggest a role of AKAP95 in DNA replication by providing a scaffold for MCM2...
  18. pmc Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract
    Christel T Freberg
    Department of Biochemistry, Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway
    Mol Biol Cell 18:1543-53. 2007
    ..We conclude that a central epigenetic reprogramming event is relaxation of chromatin at loci associated with pluripotency to create a conformation compatible with transcriptional activation...
  19. pmc Chromatin environment of histone variant H3.3 revealed by quantitative imaging and genome-scale chromatin and DNA immunoprecipitation
    Erwan Delbarre
    Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    Mol Biol Cell 21:1872-84. 2010
    ..Our data suggest that in mesenchymal stem cells, H3.3 targets lineage-priming genes with a potential for activation facilitated by H3K4me3 in facultative association with H3K27me3...
  20. ncbi request reprint Reprogramming fibroblasts to express T-cell functions using cell extracts
    Anne Mari Håkelien
    Institute of Medical Biochemistry, P O Box 1112, Blindern, University of Oslo, Oslo 0317, Norway
    Nat Biotechnol 20:460-6. 2002
    ..In vitro reprogramming of differentiated somatic cells creates possibilities for producing isogenic replacement cells for therapeutic applications...
  21. pmc Differential transcript isoform usage pre- and post-zygotic genome activation in zebrafish
    Håvard Aanes
    BasAM, Norwegian School of Veterinary Science, 0033 Dep, Oslo, Norway
    BMC Genomics 14:331. 2013
    ..Here, we perform isoform discovery and quantification on transcriptome sequences from before and after zebrafish zygotic genome activation (ZGA)...
  22. ncbi request reprint The KH-Tudor domain of a-kinase anchoring protein 149 mediates RNA-dependent self-association
    Marie Rogne
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, P O Box 1112 Blindern, 0317 Oslo, Norway
    Biochemistry 45:14980-9. 2006
    ..AKAP149 emerges as a scaffolding protein involved in the integration of intracellular signals and possibly in RNA metabolism...
  23. pmc Induction of dedifferentiation, genomewide transcriptional programming, and epigenetic reprogramming by extracts of carcinoma and embryonic stem cells
    Christel K Taranger
    Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway
    Mol Biol Cell 16:5719-35. 2005
    ..The data provide a proof-of-concept that an extract of undifferentiated carcinoma cells can elicit differentiation plasticity in an otherwise more developmentally restricted cell type...
  24. pmc Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture
    Andrew C Boquest
    Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway
    Mol Biol Cell 16:1131-41. 2005
    ....
  25. pmc Nuclear reprogramming in cell-free extracts
    Philippe Collas
    Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
    Philos Trans R Soc Lond B Biol Sci 358:1389-95. 2003
    ..The system may also constitute a powerful tool to examine the mechanisms of nuclear reprogramming, at least as they occur in vitro...
  26. ncbi request reprint Long-term in vitro, cell-type-specific genome-wide reprogramming of gene expression
    Anne Mari Håkelien
    Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
    Exp Cell Res 309:32-47. 2005
    ..Therefore, target cell-type-specific and heritable changes in gene expression, and alterations in cell function, can be promoted by extracts derived from transformed cells as well as from adult primary cells...
  27. ncbi request reprint In vitro reprogramming of nuclei and cells
    Anne Mari Håkelien
    Institute of Medical Biochemistry, University of Oslo, Oslo, Norway
    Methods Mol Biol 348:259-68. 2006
    ..The system is likely to constitute a powerful tool to examine the processes of nuclear reprogramming, at least as they occur in vitro...
  28. ncbi request reprint Isolation of stromal stem cells from human adipose tissue
    Andrew C Boquest
    Institute of Medical Biochemistry, University of Oslo, Norway
    Methods Mol Biol 325:35-46. 2006
    ..This chapter describes procedures for isolating millions of highly purified stromal stem cells from human adipose tissue and methods of establishing polyclonal and monoclonal cultures of adipose tissue-derived stem cells...
  29. doi request reprint Lineage-specific promoter DNA methylation patterns segregate adult progenitor cell types
    Anita L Sørensen
    Department of Biochemistry, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo and Norwegian Center for Stem Cell Research, Oslo, Norway
    Stem Cells Dev 19:1257-66. 2010
    ....
  30. ncbi request reprint In vitro modulation of the interaction between HA95 and LAP2beta by cAMP signaling
    Sandra B Martins
    Institute of Medical Biochemistry, University of Oslo, P O Box 1112 Blindern, 0317 Oslo, Norway
    Biochemistry 42:10456-61. 2003
    ..This suggests an additional level of regulation of a chromatin-nuclear envelope interaction in dividing cells...
  31. ncbi request reprint Modulation of cell fate using nuclear and cytoplasmic extracts
    Anne Mari Håkelien
    Institute of Medical Biochemistry, University of Oslo, Norway
    Methods Mol Biol 325:99-114. 2006
    ....
  32. doi request reprint Epigenetic complexity during the zebrafish mid-blastula transition
    Ingrid S Andersen
    Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway
    Biochem Biophys Res Commun 417:1139-44. 2012
    ..Thus enrichment in trimethylated H3K9 or H3K27 is associated with local remodeling of chromatin manifested by changes in H3K4me3 density. We propose that metagenes can provide information on the multivalency of chromatin sates...
  33. doi request reprint Mutually exclusive binding of PP1 and RNA to AKAP149 affects the mitochondrial network
    Marie Rogne
    1Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, Post Box 1112, Blindern, 0317 Oslo, Norway
    Hum Mol Genet 18:978-87. 2009
    ..A collapse of the mitochondrial network is observed upon introduction of RNA-binding deficient mutants of AKAP149, pointing to the importance of RNA tethering to the mitochondrial membrane by AKAP149 for mitochondrial distribution...
  34. doi request reprint Genetic and epigenetic instability of human bone marrow mesenchymal stem cells expanded in autologous serum or fetal bovine serum
    John Arne Dahl
    Department of Biochemistry, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, and Rikshospitalet Medical Centre, Oslo, Norway
    Int J Dev Biol 52:1033-42. 2008
    ....
  35. ncbi request reprint Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytes
    Kristine G Gaustad
    Institute of Medical Biochemistry, University of Oslo, Oslo, Norway
    Biochem Biophys Res Commun 314:420-7. 2004
    ..Cell extracts may also prove useful for investigating the molecular mechanisms of stem cell differentiation...
  36. pmc PNUTS enhances in vitro chromosome decondensation in a PP1-dependent manner
    Helga B Landsverk
    Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, P O Box 1112, Blindern, 0317 Oslo, Norway
    Biochem J 390:709-17. 2005
    ..We hypothesize that targeting of PNUTS to reforming nuclei in telophase may be a part of a signalling event promoting chromatin decondensation as cells re-enter interphase...
  37. ncbi request reprint Transient alteration of cell fate using a nuclear and cytoplasmic extract of an insulinoma cell line
    Anne Mari Håkelien
    Institute of Medical Biochemistry, University of Oslo, Oslo 0317, Norway
    Biochem Biophys Res Commun 316:834-41. 2004
    ..Because they are easily accessible, cell extracts may represent a practical source of material for investigating the mechanisms of alteration of a nuclear and cellular program...
  38. doi request reprint Expression of the myodystrophic R453W mutation of lamin A in C2C12 myoblasts causes promoter-specific and global epigenetic defects
    Anne Mari Håkelien
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
    Exp Cell Res 314:1869-80. 2008
    ..Our results argue that gene-specific and genome-wide chromatin rearrangements may constitute a molecular basis for laminopathies...
  39. doi request reprint cAMP induces autophagy via a novel pathway involving ERK, cyclin E and Beclin 1
    Hege Ugland
    Department of Biochemistry, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway
    Autophagy 7:1199-211. 2011
    ..Given the roles of deregulated autophagy in neurodegenerative disorders and cAMP as a neurogenic inducer, identification of this novel autophagocytic pathway may provide new targets for intervention against neurological disorders...
  40. doi request reprint Persistence of collagen type II synthesis and secretion in rapidly proliferating human articular chondrocytes in vitro
    Aboulghassem Shahdadfar
    Institute of Immunology, Rikshospitalet University Hospital, Oslo, Norway
    Tissue Eng Part A 14:1999-2007. 2008
    ..Thus, the CA-ECM strategy allows AC to proliferate to clinically useful numbers while maintaining COL2 synthesis and secretion. This strategy may improve tissue engineering of joint surfaces...
  41. pmc The protein phosphatase 1 regulator PNUTS is a new component of the DNA damage response
    Helga B Landsverk
    Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    EMBO Rep 11:868-75. 2010
    ..We identify the PP1c regulatory subunit PNUTS as a new and integral component of the DNA damage response involved in DNA repair...
  42. pmc Dynamics of adipogenic promoter DNA methylation during clonal culture of human adipose stem cells to senescence
    Agate Noer
    Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway
    BMC Cell Biol 8:18. 2007
    ..We examined the adipogenic differentiation potential of clones of human ASCs in early passage culture and upon senescence, and determined whether senescence was associated with changes in adipogenic promoter DNA methylation...
  43. doi request reprint A rapid micro chromatin immunoprecipitation assay (microChIP)
    John Arne Dahl
    Department of Biochemistry, Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo 0317, Norway
    Nat Protoc 3:1032-45. 2008
    ..From cell fixation to PCR-ready DNA, the procedure takes approximately 8 h for 16 ChIPs...
  44. ncbi request reprint Involvement of the catalytic subunit of protein kinase A and of HA95 in pre-mRNA splicing
    Anne Katrine Kvissel
    Department of Nutrition, University of Oslo, Oslo, Norway
    Exp Cell Res 313:2795-809. 2007
    ..Our findings demonstrate that the nuclear PKA C subunit co-locates with HA95 in SFCs and regulates pre-mRNA splicing, possibly through a cAMP-independent mechanism...
  45. pmc Lamin A/C-promoter interactions specify chromatin state-dependent transcription outcomes
    Eivind Lund
    Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, and Norwegian Center for Stem Cell Research, Blindern, 0317 Oslo, Norway
    Genome Res 23:1580-9. 2013
    ..We propose that lamin A/C acts as a modulator of local gene expression outcome through interaction with adjustable sites on promoters, and that these position-dependent transcriptional readouts may be reset upon differentiation. ..
  46. ncbi request reprint CpG methylation profiles of endothelial cell-specific gene promoter regions in adipose tissue stem cells suggest limited differentiation potential toward the endothelial cell lineage
    Andrew C Boquest
    Institute of Basic Medical Sciences, Faculty of Medicine, Department of Biochemistry, University of Oslo, Oslo, Norway
    Stem Cells 25:852-61. 2007
    ..Analysis of CpG methylation at lineage-specific promoters provides a robust assessment of epigenetic commitment of stem cells to a specific lineage...
  47. doi request reprint Epigenetic marking of the zebrafish developmental program
    Ingrid S Andersen
    Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway
    Curr Top Dev Biol 104:85-112. 2013
    ..From these observations, a concept of epigenetic prepatterning of the embryonic gene expression program prior to the onset of ZGA is emerging. The recent data collectively start shedding light on how ZGA may be programmed and regulated...
  48. doi request reprint Epigenetic regulation of nestin expression during neurogenic differentiation of adipose tissue stem cells
    Jean Luc Boulland
    Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    Stem Cells Dev 22:1042-52. 2013
    ..Our findings provide molecular evidence that the differentiation repertoire of ASCs may extend beyond mesodermal lineages...
  49. pmc DAXX-dependent supply of soluble (H3.3-H4) dimers to PML bodies pending deposition into chromatin
    Erwan Delbarre
    Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway
    Genome Res 23:440-51. 2013
    ..3-H4) dimer rather than as a tetramer. Our data support a model of DAXX-mediated recruitment of (H3.3-H4) dimers to PML bodies, which may function as triage centers for H3.3 deposition into chromatin by distinct chaperones...
  50. doi request reprint Prepatterning of developmental gene expression by modified histones before zygotic genome activation
    Leif C Lindeman
    Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, and Norwegian Center for Stem Cell Research, 0317 Oslo, Norway
    Dev Cell 21:993-1004. 2011
    ..Our data suggest that histone modifications are instructive for the developmental gene expression program...
  51. doi request reprint Chromatin states of core pluripotency-associated genes in pluripotent, multipotent and differentiated cells
    Sanna Barrand
    Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway
    Biochem Biophys Res Commun 391:762-7. 2010
    ..Establishment of distinct repression mechanisms for pluripotency-associated genes may constitute a safeguard system to prevent promiscuous reactivation during development or differentiation...
  52. pmc Stable CpG hypomethylation of adipogenic promoters in freshly isolated, cultured, and differentiated mesenchymal stem cells from adipose tissue
    Agate Noer
    Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway
    Mol Biol Cell 17:3543-56. 2006
    ..Therefore, mosaic hypomethylation of adipogenic promoters may constitute a molecular signature of ASCs, and DNA methylation does not seem to be a determinant of differentiation potential of these cells...
  53. pmc cAMP-mediated induction of cyclin E sensitizes growth-arrested adipose stem cells to DNA damage-induced apoptosis
    Hege Ugland
    Department of Biochemistry, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, N 0317 Oslo, Norway
    Mol Biol Cell 19:5082-92. 2008
    ..Our results suggest that cyclin E plays an important, cdk2-independent role in genotoxic stress-induced apoptosis in mesenchymal stem cells...
  54. doi request reprint High-resolution analysis of genetic stability of human adipose tissue stem cells cultured to senescence
    Leonardo A Meza-Zepeda
    Department of Tumor Biology, Rikshospitalet Radiumhospitalet Medical Center, Montebello, Oslo, Norway
    J Cell Mol Med 12:553-63. 2008
    ..Nonetheless, incidence of these aberrations seems to be negligible in the majority of long-term ASC cultures, at least under the culture conditions used here...
  55. ncbi request reprint Epigenetic programming of mesenchymal stem cells from human adipose tissue
    Andrew C Boquest
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, Blindern, 0317 Oslo, Norway
    Stem Cell Rev 2:319-29. 2006
    ..Novel attempts at reprogramming the epigenome of MSCs have been initiated to enhance the differentiation capacity of these cells...
  56. pmc Fast genomic muChIP-chip from 1,000 cells
    John Arne Dahl
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway
    Genome Biol 10:R13. 2009
    ..muChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed...
  57. ncbi request reprint Q2ChIP, a quick and quantitative chromatin immunoprecipitation assay, unravels epigenetic dynamics of developmentally regulated genes in human carcinoma cells
    John Arne Dahl
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, Oslo, Norway
    Stem Cells 25:1037-46. 2007
    ..The results demonstrate ordered chromatin rearrangement on developmentally regulated promoters upon differentiation...
  58. doi request reprint Promoter-exon relationship of H3 lysine 9, 27, 36 and 79 methylation on pluripotency-associated genes
    Sanna Barrand
    Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway
    Biochem Biophys Res Commun 401:611-7. 2010
    ..mRNA levels are however affected, raising the hypothesis of a role of SetD2 on transcription elongation and/or termination...
  59. doi request reprint Fish'n ChIPs: chromatin immunoprecipitation in the zebrafish embryo
    Leif C Lindeman
    Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    Methods Mol Biol 567:75-86. 2009
    ..We also incorporated key steps from a recently published ChIP assay for small cell numbers. We report here a protocol for immunoprecipitation of modified histones from mid-term blastula zebrafish embryos...
  60. ncbi request reprint Novel approaches to transdifferentiation
    Anne Mari Håkelien
    Institute of Medical Biochemistry, University of Oslo, Blindern, Oslo, Norway
    Cloning Stem Cells 4:379-87. 2002
    ..The system is also likely to constitute a powerful tool to examine the mechanisms of nuclear reprogramming as they occur in vitro...
  61. ncbi request reprint Association of PP1 with its regulatory subunit AKAP149 is regulated by serine phosphorylation flanking the RVXF motif of AKAP149
    Thomas Küntziger
    Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
    Biochemistry 45:5868-77. 2006
    ..AKAP149 emerges as a scaffolding protein for multiple protein kinases and phosphatases that may be involved in the integration of intracellular signals that converge at the nuclear envelope...
  62. ncbi request reprint A quick and quantitative chromatin immunoprecipitation assay for small cell samples
    John Arne Dahl
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, Blindern, Oslo, Norway
    Front Biosci 12:4925-31. 2007
    ..This communication describes all steps of the Q2ChIP procedure as it is carried out in our laboratory...
  63. ncbi request reprint AKAP149 is a novel PP1 specifier required to maintain nuclear envelope integrity in G1 phase
    Rikke L Steen
    Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
    J Cell Sci 116:2237-46. 2003
    ..This correlates with the induction of a G1 arrest and, ultimately, apoptosis. We propose that AKAP149-regulated PP1 activity at the NE during G1 is required to maintain nuclear integrity and cell survival...
  64. doi request reprint MicroChIP: chromatin immunoprecipitation for small cell numbers
    John Arne Dahl
    Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    Methods Mol Biol 567:59-74. 2009
    ..We describe here a rapid micro (micro)ChIP assay suited for multiple parallel ChIPs from a single chromatin batch from 1,000 cells. The assay is also applicable to a single immunoprecipitation from 100 cells...
  65. pmc RNA profiles of porcine embryos during genome activation reveal complex metabolic switch sensitive to in vitro conditions
    Olga Østrup
    Institute for Basic Medical Sciences, Faculty of Medicine, University of Oslo and Norwegian Center for Stem Cell Research, Oslo, Norway
    PLoS ONE 8:e61547. 2013
    ..Moreover, comparison with mouse and human embryos showed striking overlap in functional annotation of transcripts during the EGA, suggesting conserved basic mechanisms regulating establishment of totipotency in mammalian development...
  66. ncbi request reprint Protein phosphatase 1 regulators in DNA damage signaling
    Thomas Küntziger
    Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway
    Cell Cycle 10:1356-62. 2011
    ..Here we review recent progress regarding the involvement of PP1 and its binding partners in DNA damage signaling...
  67. doi request reprint The state-of-the-art of chromatin immunoprecipitation
    Philippe Collas
    Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    Methods Mol Biol 567:1-25. 2009
    ..This review highlights the variations on the theme of the ChIP assay, the various detection methods applied downstream of ChIP, and examples of their application...
  68. pmc HA95 and LAP2 beta mediate a novel chromatin-nuclear envelope interaction implicated in initiation of DNA replication
    Sandra Martins
    Institute of Medical Biochemistry, University of Oslo, Oslo 0317, Norway
    J Cell Biol 160:177-88. 2003
    ..Rescue of Cdc6 degradation with proteasome inhibitors restores replication. We propose that an interaction of LAP2beta, or LAP2 proteins, with HA95 is involved in the control of initiation of DNA replication...
  69. doi request reprint Immunoprecipitation of methylated DNA
    Anita L Sørensen
    Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    Methods Mol Biol 567:249-62. 2009
    ..The genomic site of interest can be detected by PCR, hybridization to DNA arrays, or by direct sequencing. This chapter describes the MeDIP protocol and quality control tests that should be performed throughout the procedure...
  70. ncbi request reprint Teaching cells new tricks
    Philippe Collas
    Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
    Trends Biotechnol 21:354-61. 2003
    ..Considerable efforts remain to unravel the molecular processes underlying nuclear reprogramming and evaluate stable of the changes in reprogrammed cells...
  71. doi request reprint Obtaining freshly isolated and cultured mesenchymal stem cells from human adipose tissue
    Andrew C Boquest
    Institute of Basic Medical Sciences and Norwegian Center for Stem Cell Research, University of Oslo, Oslo, Norway
    Methods Mol Biol 879:269-78. 2012
    ..In this chapter, we outline procedures for isolating large numbers of highly purified MSCs from human adipose tissue in their native, uncultured form and methods for their subsequent expansion and differentiation in vitro...
  72. ncbi request reprint Novel approaches to epigenetic reprogramming of somatic cells
    Philippe Collas
    Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, Oslo, Norway
    Cloning Stem Cells 9:26-32. 2007
  73. pmc Reprogrammed gene expression in a somatic cell-free extract
    Helga B Landsverk
    Institute of Medical Biochemistry, PO Box 1112 Blindern, University of Oslo, Oslo 0317, Norway
    EMBO Rep 3:384-9. 2002
    ..In vitro reprogramming may be useful for investigating regulation of gene expression and for producing replacement cells for the treatment of a wide variety of diseases...
  74. ncbi request reprint DNA-containing extracellular 50-nm particles in the ileal Peyer's patch of sheep
    Philippe Collas
    Institute of Medical Biochemistry, University of Oslo, Norway
    Eur J Cell Biol 81:69-76. 2002
    ..This raises the possibility of a novel form of communication between cells mediated by nucleic acids...
  75. ncbi request reprint Cell extract-derived differentiation of embryonic stem cells
    Mingde Qin
    Tissue Engineering and Regenerative Medicine Centre, Imperial College Faculty of Medicine, Chelsea and Westminster Campus, Fulham Road, London SW10 9NH, UK
    Stem Cells 23:712-8. 2005
    ..Our findings establish that ESCs can be differentiated in vitro using cellular extracts. This model provides a tool for analysis of the key factors involved in the differentiation of ESCs to type II pneumocytes...
  76. ncbi request reprint Cloned calves from chromatin remodeled in vitro
    Eddie J Sullivan
    Hematech, LLC, Sioux Falls, South Dakota 57106, USA
    Biol Reprod 70:146-53. 2004
    ..This procedure should be useful for investigating mechanisms of nuclear reprogramming and for making improvements in the efficiency of mammalian cloning...
  77. ncbi request reprint Targeted gene delivery to differentiated skeletal muscle: a tool to study dedifferentiation
    Jamie I Morrison
    Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
    Dev Dyn 236:481-8. 2007
    ..The combination of the transfection protocol with live imaging allows a time- and cost-effective screen of candidate genes in terminally differentiated muscle cells of both amphibian and mammalian origin...
  78. ncbi request reprint Differential regulation of maternal and paternal chromosome condensation in mitotic zygotes
    Jacqueline Bomar
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst 01003, USA
    J Cell Sci 115:2931-40. 2002
    ..We propose a concept whereby condensation of chromosomes in gametes, zygotes and somatic cells involves related but distinct mechanisms...
  79. ncbi request reprint Architectural defects in pronuclei of mouse nuclear transplant embryos
    Pedro N Moreira
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA
    J Cell Sci 116:3713-20. 2003
    ..We propose that somatic nuclear reprogramming deficiencies by NT might emanate from, at least in part, failure to remodel the somatic nucleus morphologically into a functional embryonic nucleus...