Research Topics
Genomes and Genes
| Philippe CollasSummaryAffiliation: University of Oslo Country: Norway Publications
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Detail Information
Publications
Oslo Epigenetics Symposium 2012. Oslo, Norway, 8-9 November 2012Philippe Collas
Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
Epigenomics 5:29-32. 2013....- A chromatin immunoprecipitation protocol for small cell numbersPhilippe Collas
University of Oslo, Institute of Basic Medical Sciences and Norwegian Center for Stem Cell Research, Oslo, Norway
Methods Mol Biol 791:179-93. 2011..This chapter describes a micro (μ)ChIP procedure for multiple parallel ChIPs from a single chromatin batch from 1,000 cells... 
On the way to reprogramming cells to pluripotency using cell-free extractsPhilippe Collas
Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
Reprod Biomed Online 12:762-70. 2006..These approaches include the fusion of differentiated cells with embryonic stem cells and the use of extract from pluripotent cells to reprogramme differentiated cells into multipotent or pluripotent cells...- Epigenetic reprogramming of nuclei using cell extractsPhilippe Collas
Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, Blindern, Oslo 0317, Norway
Stem Cell Rev 2:309-17. 2006..Limitations of current extract-based reprogramming approaches are also addressed... - Programming differentiation potential in mesenchymal stem cellsPhilippe Collas
Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway
Epigenetics 5:476-82. 2010..Additional regulatory layers need to be examined to be able to explain cell fate commitment and ultimately predict cell fate... - The current state of chromatin immunoprecipitationPhilippe Collas
Institute of Basic Medical Sciences, Faculty of Medicine, Norwegian Center for Stem Cell Research, University of Oslo, Oslo, Norway
Mol Biotechnol 45:87-100. 2010..This review highlights the variations on the theme of the ChIP assay, the various detection methods applied downstream of ChIP, and examples of their application... - Epigenetic states in stem cellsPhilippe Collas
Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, Norway
Biochim Biophys Acta 1790:900-5. 2009..We summarize here the current view of how specific combinations of epigenetic marks may define the pluripotent state... - Programming the genome in embryonic and somatic stem cellsPhilippe Collas
Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway
J Cell Mol Med 11:602-20. 2007..The combinatorial association of epigenetic marks on developmentally regulated and lineage-specifying genes in undifferentiated cells seems to define a pluripotent state... - Chop it, ChIP it, check it: the current status of chromatin immunoprecipitationPhilippe Collas
Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
Front Biosci 13:929-43. 2008..Finally, we speculate on future perspectives opened by the combination of emerging ChIP-related technologies... 
Tiling histone H3 lysine 4 and 27 methylation in zebrafish using high-density microarraysLeif C Lindeman
Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, and Norwegian Center for Stem Cell Research, Oslo, Norway
PLoS ONE 5:e15651. 2010..Many studies have been carried out in mice and humans; however limited high-resolution information exists to date for non-mammalian vertebrate species...- Promoter DNA methylation patterns of differentiated cells are largely programmed at the progenitor stageAnita L Sørensen
Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, and Norwegian Center for Stem Cell Research, 0317 Oslo, Norway
Mol Biol Cell 21:2066-77. 2010..We infer that methylation state of lineage-specific promoters in MSCs is not a primary determinant of differentiation capacity. Our results support the view of a common origin of mesenchymal progenitors... - Histone H3 modifications associated with differentiation and long-term culture of mesenchymal adipose stem cellsAgate Noer
Department of Biochemistry, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway
Stem Cells Dev 18:725-36. 2009..This correlates with global cellular Ezh2 increase and H3K9 deacetylation. Promoter targeting by Ezh2 and Bmi1 in late passage ASCs suggests the establishment of a polycomb-mediated epigenetic program aiming at repressing transcription... - Chromatin states of developmentally-regulated genes revealed by DNA and histone methylation patterns in zebrafish embryosLeif C Lindeman
Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, and Norwegian Center for Stem Cell Research, Oslo, Norway
Int J Dev Biol 54:803-13. 2010..Our results suggest that differentially expressed embryonic genes are regulated by various patterns of histone modifications on unmethylated DNA, which create a developmentally permissive chromatin state... - Protein kinase A-anchoring protein AKAP95 interacts with MCM2, a regulator of DNA replicationTurid Eide
Department of Medical Biochemistry, University of Oslo, P O Box 1112 Blindern, 0317 Oslo, Norway
J Biol Chem 278:26750-6. 2003..Recombinant AKAP95 restores intranuclear MCM2 and replication in a dose-dependent manner. Our results suggest a role of AKAP95 in DNA replication by providing a scaffold for MCM2... - In vitro reprogramming of nuclei and cellsAnne Mari Håkelien
Institute of Medical Biochemistry, University of Oslo, Oslo, Norway
Methods Mol Biol 348:259-68. 2006..The system is likely to constitute a powerful tool to examine the processes of nuclear reprogramming, at least as they occur in vitro... - Long-term in vitro, cell-type-specific genome-wide reprogramming of gene expressionAnne Mari Håkelien
Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
Exp Cell Res 309:32-47. 2005..Therefore, target cell-type-specific and heritable changes in gene expression, and alterations in cell function, can be promoted by extracts derived from transformed cells as well as from adult primary cells... - Chromatin environment of histone variant H3.3 revealed by quantitative imaging and genome-scale chromatin and DNA immunoprecipitationErwan Delbarre
Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
Mol Biol Cell 21:1872-84. 2010..Our data suggest that in mesenchymal stem cells, H3.3 targets lineage-priming genes with a potential for activation facilitated by H3K4me3 in facultative association with H3K27me3... - The KH-Tudor domain of a-kinase anchoring protein 149 mediates RNA-dependent self-associationMarie Rogne
Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, P.O. Box 1112 Blindern, 0317 Oslo, Norway
Biochemistry 45:14980-9. 2006..AKAP149 emerges as a scaffolding protein involved in the integration of intracellular signals and possibly in RNA metabolism... - Induction of dedifferentiation, genomewide transcriptional programming, and epigenetic reprogramming by extracts of carcinoma and embryonic stem cellsChristel K Taranger
Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway
Mol Biol Cell 16:5719-35. 2005..The data provide a proof-of-concept that an extract of undifferentiated carcinoma cells can elicit differentiation plasticity in an otherwise more developmentally restricted cell type... - Nuclear reprogramming in cell-free extractsPhilippe Collas
Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
Philos Trans R Soc Lond B Biol Sci 358:1389-95. 2003..The system may also constitute a powerful tool to examine the mechanisms of nuclear reprogramming, at least as they occur in vitro... - Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell cultureAndrew C Boquest
Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway
Mol Biol Cell 16:1131-41. 2005.... - Modulation of cell fate using nuclear and cytoplasmic extractsAnne Mari Håkelien
Institute of Medical Biochemistry, University of Oslo, Norway
Methods Mol Biol 325:99-114. 2006.... - Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extractChristel T Freberg
Department of Biochemistry, Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway
Mol Biol Cell 18:1543-53. 2007..We conclude that a central epigenetic reprogramming event is relaxation of chromatin at loci associated with pluripotency to create a conformation compatible with transcriptional activation... - In vitro modulation of the interaction between HA95 and LAP2beta by cAMP signalingSandra B Martins
Institute of Medical Biochemistry, University of Oslo, P O Box 1112 Blindern, 0317 Oslo, Norway
Biochemistry 42:10456-61. 2003..This suggests an additional level of regulation of a chromatin-nuclear envelope interaction in dividing cells... - Isolation of stromal stem cells from human adipose tissueAndrew C Boquest
Institute of Medical Biochemistry, University of Oslo, Norway
Methods Mol Biol 325:35-46. 2006..This chapter describes procedures for isolating millions of highly purified stromal stem cells from human adipose tissue and methods of establishing polyclonal and monoclonal cultures of adipose tissue-derived stem cells... - Lineage-specific promoter DNA methylation patterns segregate adult progenitor cell typesAnita L Sørensen
Department of Biochemistry, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo and Norwegian Center for Stem Cell Research, Oslo, Norway
Stem Cells Dev 19:1257-66. 2010.... - Transient alteration of cell fate using a nuclear and cytoplasmic extract of an insulinoma cell lineAnne Mari Håkelien
Institute of Medical Biochemistry, University of Oslo, Oslo 0317, Norway
Biochem Biophys Res Commun 316:834-41. 2004..Because they are easily accessible, cell extracts may represent a practical source of material for investigating the mechanisms of alteration of a nuclear and cellular program... - Epigenetic complexity during the zebrafish mid-blastula transitionIngrid S Andersen
Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway
Biochem Biophys Res Commun 417:1139-44. 2012..Thus enrichment in trimethylated H3K9 or H3K27 is associated with local remodeling of chromatin manifested by changes in H3K4me3 density. We propose that metagenes can provide information on the multivalency of chromatin sates... - Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytesKristine G Gaustad
Institute of Medical Biochemistry, University of Oslo, Oslo, Norway
Biochem Biophys Res Commun 314:420-7. 2004..Cell extracts may also prove useful for investigating the molecular mechanisms of stem cell differentiation... - Genetic and epigenetic instability of human bone marrow mesenchymal stem cells expanded in autologous serum or fetal bovine serumJohn Arne Dahl
Department of Biochemistry, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, and Rikshospitalet Medical Centre, Oslo, Norway
Int J Dev Biol 52:1033-42. 2008.... - Reprogramming fibroblasts to express T-cell functions using cell extractsAnne Mari Håkelien
Institute of Medical Biochemistry, P O Box 1112, Blindern, University of Oslo, Oslo 0317, Norway
Nat Biotechnol 20:460-6. 2002..In vitro reprogramming of differentiated somatic cells creates possibilities for producing isogenic replacement cells for therapeutic applications... - Mutually exclusive binding of PP1 and RNA to AKAP149 affects the mitochondrial networkMarie Rogne
1Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, Post Box 1112, Blindern, 0317 Oslo, Norway
Hum Mol Genet 18:978-87. 2009..A collapse of the mitochondrial network is observed upon introduction of RNA-binding deficient mutants of AKAP149, pointing to the importance of RNA tethering to the mitochondrial membrane by AKAP149 for mitochondrial distribution... - Expression of the myodystrophic R453W mutation of lamin A in C2C12 myoblasts causes promoter-specific and global epigenetic defectsAnne Mari Håkelien
Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
Exp Cell Res 314:1869-80. 2008..Our results argue that gene-specific and genome-wide chromatin rearrangements may constitute a molecular basis for laminopathies... - cAMP induces autophagy via a novel pathway involving ERK, cyclin E and Beclin 1Hege Ugland
Department of Biochemistry, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway
Autophagy 7:1199-211. 2011..Given the roles of deregulated autophagy in neurodegenerative disorders and cAMP as a neurogenic inducer, identification of this novel autophagocytic pathway may provide new targets for intervention against neurological disorders... - A rapid micro chromatin immunoprecipitation assay (microChIP)John Arne Dahl
Department of Biochemistry, Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo 0317, Norway
Nat Protoc 3:1032-45. 2008..From cell fixation to PCR-ready DNA, the procedure takes approximately 8 h for 16 ChIPs... - The protein phosphatase 1 regulator PNUTS is a new component of the DNA damage responseHelga B Landsverk
Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
EMBO Rep 11:868-75. 2010..We identify the PP1c regulatory subunit PNUTS as a new and integral component of the DNA damage response involved in DNA repair... - Persistence of collagen type II synthesis and secretion in rapidly proliferating human articular chondrocytes in vitroAboulghassem Shahdadfar
Institute of Immunology, Rikshospitalet University Hospital, Oslo, Norway
Tissue Eng Part A 14:1999-2007. 2008..Thus, the CA-ECM strategy allows AC to proliferate to clinically useful numbers while maintaining COL2 synthesis and secretion. This strategy may improve tissue engineering of joint surfaces... - Involvement of the catalytic subunit of protein kinase A and of HA95 in pre-mRNA splicingAnne Katrine Kvissel
Department of Nutrition, University of Oslo, Oslo, Norway
Exp Cell Res 313:2795-809. 2007..Our findings demonstrate that the nuclear PKA C subunit co-locates with HA95 in SFCs and regulates pre-mRNA splicing, possibly through a cAMP-independent mechanism... - Dynamics of adipogenic promoter DNA methylation during clonal culture of human adipose stem cells to senescenceAgate Noer
Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway
BMC Cell Biol 8:18. 2007..We examined the adipogenic differentiation potential of clones of human ASCs in early passage culture and upon senescence, and determined whether senescence was associated with changes in adipogenic promoter DNA methylation... - Histone H3 lysine 27 methylation asymmetry on developmentally-regulated promoters distinguish the first two lineages in mouse preimplantation embryosJohn Arne Dahl
Institute of Basic Medical Sciences, University of Oslo and Norwegian Center for Stem Cell Research, Oslo, Norway
PLoS ONE 5:e9150. 2010..Our results show histone trimethylation asymmetry on promoters in the first two developmental lineages, and highlight an epigenetic skewing associated with embryonic stem cell derivation... - Prepatterning of developmental gene expression by modified histones before zygotic genome activationLeif C Lindeman
Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, and Norwegian Center for Stem Cell Research, 0317 Oslo, Norway
Dev Cell 21:993-1004. 2011..Our data suggest that histone modifications are instructive for the developmental gene expression program... - Chromatin states of core pluripotency-associated genes in pluripotent, multipotent and differentiated cellsSanna Barrand
Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway
Biochem Biophys Res Commun 391:762-7. 2010..Establishment of distinct repression mechanisms for pluripotency-associated genes may constitute a safeguard system to prevent promiscuous reactivation during development or differentiation... - Stable CpG hypomethylation of adipogenic promoters in freshly isolated, cultured, and differentiated mesenchymal stem cells from adipose tissueAgate Noer
Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway
Mol Biol Cell 17:3543-56. 2006..Therefore, mosaic hypomethylation of adipogenic promoters may constitute a molecular signature of ASCs, and DNA methylation does not seem to be a determinant of differentiation potential of these cells... - PNUTS enhances in vitro chromosome decondensation in a PP1-dependent mannerHelga B Landsverk
Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, P O Box 1112, Blindern, 0317 Oslo, Norway
Biochem J 390:709-17. 2005..We hypothesize that targeting of PNUTS to reforming nuclei in telophase may be a part of a signalling event promoting chromatin decondensation as cells re-enter interphase... - cAMP-mediated induction of cyclin E sensitizes growth-arrested adipose stem cells to DNA damage-induced apoptosisHege Ugland
Department of Biochemistry, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, N 0317 Oslo, Norway
Mol Biol Cell 19:5082-92. 2008..Our results suggest that cyclin E plays an important, cdk2-independent role in genotoxic stress-induced apoptosis in mesenchymal stem cells... - Epigenetic programming of mesenchymal stem cells from human adipose tissueAndrew C Boquest
Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, Blindern, 0317 Oslo, Norway
Stem Cell Rev 2:319-29. 2006..Novel attempts at reprogramming the epigenome of MSCs have been initiated to enhance the differentiation capacity of these cells... - High-resolution analysis of genetic stability of human adipose tissue stem cells cultured to senescenceLeonardo A Meza-Zepeda
Department of Tumor Biology, Rikshospitalet Radiumhospitalet Medical Center, Montebello, Oslo, Norway
J Cell Mol Med 12:553-63. 2008..Nonetheless, incidence of these aberrations seems to be negligible in the majority of long-term ASC cultures, at least under the culture conditions used here... - Novel approaches to transdifferentiationAnne Mari Håkelien
Institute of Medical Biochemistry, University of Oslo, Blindern, Oslo, Norway
Cloning Stem Cells 4:379-87. 2002..The system is also likely to constitute a powerful tool to examine the mechanisms of nuclear reprogramming as they occur in vitro... - Association of PP1 with its regulatory subunit AKAP149 is regulated by serine phosphorylation flanking the RVXF motif of AKAP149Thomas Küntziger
Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
Biochemistry 45:5868-77. 2006..AKAP149 emerges as a scaffolding protein for multiple protein kinases and phosphatases that may be involved in the integration of intracellular signals that converge at the nuclear envelope... - Fast genomic muChIP-chip from 1,000 cellsJohn Arne Dahl
Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway
Genome Biol 10:R13. 2009..muChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed... - Promoter-exon relationship of H3 lysine 9, 27, 36 and 79 methylation on pluripotency-associated genesSanna Barrand
Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway
Biochem Biophys Res Commun 401:611-7. 2010..mRNA levels are however affected, raising the hypothesis of a role of SetD2 on transcription elongation and/or termination... - Q2ChIP, a quick and quantitative chromatin immunoprecipitation assay, unravels epigenetic dynamics of developmentally regulated genes in human carcinoma cellsJohn Arne Dahl
Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, Oslo, Norway
Stem Cells 25:1037-46. 2007..The results demonstrate ordered chromatin rearrangement on developmentally regulated promoters upon differentiation... - MicroChIP: chromatin immunoprecipitation for small cell numbersJohn Arne Dahl
Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
Methods Mol Biol 567:59-74. 2009..We describe here a rapid micro (micro)ChIP assay suited for multiple parallel ChIPs from a single chromatin batch from 1,000 cells. The assay is also applicable to a single immunoprecipitation from 100 cells... - AKAP149 is a novel PP1 specifier required to maintain nuclear envelope integrity in G1 phaseRikke L Steen
Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
J Cell Sci 116:2237-46. 2003..This correlates with the induction of a G1 arrest and, ultimately, apoptosis. We propose that AKAP149-regulated PP1 activity at the NE during G1 is required to maintain nuclear integrity and cell survival... - A quick and quantitative chromatin immunoprecipitation assay for small cell samplesJohn Arne Dahl
Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, Blindern, Oslo, Norway
Front Biosci 12:4925-31. 2007..This communication describes all steps of the Q2ChIP procedure as it is carried out in our laboratory... - CpG methylation profiles of endothelial cell-specific gene promoter regions in adipose tissue stem cells suggest limited differentiation potential toward the endothelial cell lineageAndrew C Boquest
Institute of Basic Medical Sciences, Faculty of Medicine, Department of Biochemistry, University of Oslo, Oslo, Norway
Stem Cells 25:852-61. 2007..Analysis of CpG methylation at lineage-specific promoters provides a robust assessment of epigenetic commitment of stem cells to a specific lineage... - Epigenetic marking of the zebrafish developmental programIngrid S Andersen
Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway Norwegian Center for Stem Cell Research, University of Oslo, Oslo, Norway
Curr Top Dev Biol 104:85-112. 2013..From these observations, a concept of epigenetic prepatterning of the embryonic gene expression program prior to the onset of ZGA is emerging. The recent data collectively start shedding light on how ZGA may be programmed and regulated... - Protein phosphatase 1 regulators in DNA damage signalingThomas Küntziger
Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway
Cell Cycle 10:1356-62. 2011..Here we review recent progress regarding the involvement of PP1 and its binding partners in DNA damage signaling... - The state-of-the-art of chromatin immunoprecipitationPhilippe Collas
Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
Methods Mol Biol 567:1-25. 2009..This review highlights the variations on the theme of the ChIP assay, the various detection methods applied downstream of ChIP, and examples of their application... - MicroChIP--a rapid micro chromatin immunoprecipitation assay for small cell samples and biopsiesJohn Arne Dahl
Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway
Nucleic Acids Res 36:e15. 2008..muChIP creates possibilities for multiple parallel and rapid transcription factor binding and epigenetic analyses of rare cell and tissue samples... - Teaching cells new tricksPhilippe Collas
Institute of Medical Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo, Norway
Trends Biotechnol 21:354-61. 2003..Considerable efforts remain to unravel the molecular processes underlying nuclear reprogramming and evaluate stable of the changes in reprogrammed cells... - HA95 and LAP2 beta mediate a novel chromatin-nuclear envelope interaction implicated in initiation of DNA replicationSandra Martins
Institute of Medical Biochemistry, University of Oslo, Oslo 0317, Norway
J Cell Biol 160:177-88. 2003..Rescue of Cdc6 degradation with proteasome inhibitors restores replication. We propose that an interaction of LAP2beta, or LAP2 proteins, with HA95 is involved in the control of initiation of DNA replication... - Immunoprecipitation of methylated DNAAnita L Sørensen
Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
Methods Mol Biol 567:249-62. 2009..The genomic site of interest can be detected by PCR, hybridization to DNA arrays, or by direct sequencing. This chapter describes the MeDIP protocol and quality control tests that should be performed throughout the procedure... - Obtaining freshly isolated and cultured mesenchymal stem cells from human adipose tissueAndrew C Boquest
Institute of Basic Medical Sciences and Norwegian Center for Stem Cell Research, University of Oslo, Oslo, Norway
Methods Mol Biol 879:269-78. 2012..In this chapter, we outline procedures for isolating large numbers of highly purified MSCs from human adipose tissue in their native, uncultured form and methods for their subsequent expansion and differentiation in vitro... - Fish'n ChIPs: chromatin immunoprecipitation in the zebrafish embryoLeif C Lindeman
Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
Methods Mol Biol 567:75-86. 2009..We also incorporated key steps from a recently published ChIP assay for small cell numbers. We report here a protocol for immunoprecipitation of modified histones from mid-term blastula zebrafish embryos... - Novel approaches to epigenetic reprogramming of somatic cellsPhilippe Collas
Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, Oslo, Norway
Cloning Stem Cells 9:26-32. 2007 - Reprogrammed gene expression in a somatic cell-free extractHelga B Landsverk
Institute of Medical Biochemistry, PO Box 1112 Blindern, University of Oslo, Oslo 0317, Norway
EMBO Rep 3:384-9. 2002..In vitro reprogramming may be useful for investigating regulation of gene expression and for producing replacement cells for the treatment of a wide variety of diseases... - DNA-containing extracellular 50-nm particles in the ileal Peyer's patch of sheepPhilippe Collas
Institute of Medical Biochemistry, University of Oslo, Norway
Eur J Cell Biol 81:69-76. 2002..This raises the possibility of a novel form of communication between cells mediated by nucleic acids... - Cloned calves from chromatin remodeled in vitroEddie J Sullivan
Hematech, LLC, Sioux Falls, South Dakota 57106, USA
Biol Reprod 70:146-53. 2004..This procedure should be useful for investigating mechanisms of nuclear reprogramming and for making improvements in the efficiency of mammalian cloning... - Cell extract-derived differentiation of embryonic stem cellsMingde Qin
Tissue Engineering and Regenerative Medicine Centre, Imperial College Faculty of Medicine, Chelsea and Westminster Campus, Fulham Road, London SW10 9NH, UK
Stem Cells 23:712-8. 2005..Our findings establish that ESCs can be differentiated in vitro using cellular extracts. This model provides a tool for analysis of the key factors involved in the differentiation of ESCs to type II pneumocytes... - Differential regulation of maternal and paternal chromosome condensation in mitotic zygotesJacqueline Bomar
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst 01003, USA
J Cell Sci 115:2931-40. 2002..We propose a concept whereby condensation of chromosomes in gametes, zygotes and somatic cells involves related but distinct mechanisms... - Targeted gene delivery to differentiated skeletal muscle: a tool to study dedifferentiationJamie I Morrison
Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Dev Dyn 236:481-8. 2007..The combination of the transfection protocol with live imaging allows a time- and cost-effective screen of candidate genes in terminally differentiated muscle cells of both amphibian and mammalian origin... - Architectural defects in pronuclei of mouse nuclear transplant embryosPedro N Moreira
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA
J Cell Sci 116:3713-20. 2003..We propose that somatic nuclear reprogramming deficiencies by NT might emanate from, at least in part, failure to remodel the somatic nucleus morphologically into a functional embryonic nucleus...