Affiliation: University of Oslo
Ivanauskiene K, Delbarre E, McGhie J, Küntziger T, Wong L, Collas P. The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin and is important for telomere integrity. Genome Res. 2014;24:1584-94 pubmed publisher
..3 by dedicated chaperones. Our results also suggest that telomere stability relies on mechanisms ensuring proper histone supply and routing. ..
Collas P. A chromatin immunoprecipitation protocol for small cell numbers. Methods Mol Biol. 2011;791:179-93 pubmed publisher
..This chapter describes a micro (?)ChIP procedure for multiple parallel ChIPs from a single chromatin batch from 1,000 cells. ..
Shah A, Oldenburg A, Collas P. A hyper-dynamic nature of bivalent promoter states underlies coordinated developmental gene expression modules. BMC Genomics. 2014;15:1186 pubmed publisher
..Our results reveal the highly dynamic nature of bivalent promoter states within the adipogenic lineage. The data constitute a valuable resource enabling the assessment of possibilities to alter the adipogenic program. ..
Collas P. The current state of chromatin immunoprecipitation. Mol Biotechnol. 2010;45:87-100 pubmed publisher
..This review highlights the variations on the theme of the ChIP assay, the various detection methods applied downstream of ChIP, and examples of their application. ..
Collas P. Programming differentiation potential in mesenchymal stem cells. Epigenetics. 2010;5:476-82 pubmed publisher
..Additional regulatory layers need to be examined to be able to explain cell fate commitment and ultimately predict cell fate. ..
Aanes H, Østrup O, Andersen I, Moen L, Mathavan S, Collas P, et al
. Differential transcript isoform usage pre- and post-zygotic genome activation in zebrafish. BMC Genomics. 2013;14:331 pubmed publisher
..Our data display an array of isoform-related functional changes and represent a valuable resource complementary to existing early embryo transcriptomes. ..
Ostrup O, Reiner A, AlestrÃ¶m P, Collas P. The specific alteration of histone methylation profiles by DZNep during early zebrafish development. Biochim Biophys Acta. 2014;1839:1307-15 pubmed publisher
..Altered H3K9me3 profiles were associated with severe neuronal and cranial phenotypes at day 4-5 post-fertilization. Thus, DZNep was shown to affect enrichment patterns of H3K27me3 and H3K9me3 at promoters in a gene-specific manner. ..
Oldenburg A, Collas P. Mapping Nuclear Lamin-Genome Interactions by Chromatin Immunoprecipitation of Nuclear Lamins. Methods Mol Biol. 2016;1411:315-24 pubmed publisher
..The protocol is compatible with polymerase chain reaction and high-throughput sequencing analysis of the co-immunoprecipitated DNA. ..